Reviewed, UniProtKB/Swiss-Prot P0AAF3 (ARAG_ECOLI)

Names and origin

Protein names

Recommended name:
    Arabinose import ATP-binding protein araG
    EC=3.6.3.17

Gene names

Name:	araG
Ordered Locus Names:	b1900, JW1888

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Part of the ABC transporter complex araFGH involved in arabinose import. Responsible for energy coupling to the transport system Probable.
Catalytic activity	ATP + H2O + monosaccharide(Out) = ADP + phosphate + monosaccharide(In). HAMAP MF_01721
Subunit structure	The complex is composed of two ATP-binding proteins (araG), two transmembrane proteins (araH) and a solute-binding protein (araF) Probable.
Subcellular location	Cell inner membrane; Peripheral membrane protein. Ref.5
Sequence similarities	Belongs to the ABC transporter family. Arabinose importer (TC 3.A.1.2.2) subfamily. [View classification] Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-arabinose transport Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW L-arabinose-importing ATPase activity Inferred from electronic annotation. Source: InterPro sugar:hydrogen symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Arabinose import ATP-binding protein araG HAMAP MF_01721		PRO_0000091936
Regions
	Domain	8 – 243	236	ABC transporter 1
	Domain	256 – 499	244	ABC transporter 2
	Nucleotide binding	40 – 47	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AAF3-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 23B401BCF34CFA3A	FASTA	504	55,018
	10 20 30 40 50 60 MQQSTPYLSF RGIGKTFPGV KALTDISFDC YAGQVHALMG ENGAGKSTLL KILSGNYAPT 70 80 90 100 110 120 TGSVVINGQE MSFSDTTAAL NAGVAIIYQE LHLVPEMTVA ENIYLGQLPH KGGIVNRSLL 130 140 150 160 170 180 NYEAGLQLKH LGMDIDPDTP LKYLSIGQWQ MVEIAKALAR NAKIIAFDEP TSSLSAREID 190 200 210 220 230 240 NLFRVIRELR KEGRVILYVS HRMEEIFALS DAITVFKDGR YVKTFTDMQQ VDHDALVQAM 250 260 270 280 290 300 VGRDIGDIYG WQPRSYGEER LRLDAVKAPG VRTPISLAVR SGEIVGLFGL VGAGRSELMK 310 320 330 340 350 360 GMFGGTQITA GQVYIDQQPI DIRKPSHAIA AGMMLCPEDR KAEGIIPVHS VRDNINISAR 370 380 390 400 410 420 RKHVLGGCVI NNGWEENNAD HHIRSLNIKT PGAEQLIMNL SGGNQQKAIL GRWLSEEMKV 430 440 450 460 470 480 ILLDEPTRGI DVGAKHEIYN VIYALAAQGV AVLFASSDLP EVLGVADRIV VMREGEIAGE 490 500 LLHEQADERQ ALSLAMPKVS QAVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"High-affinity L-arabinose transport operon. Nucleotide sequence and analysis of gene products." Scripture J.B., Voelker C., Miller S., O'Donnell R.T., Polgar L., Rade J., Horazdovsky B.F., Hogg R.W. J. Mol. Biol. 197:37-46(1987) [PubMed: 2445996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BEK 180.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"L-arabinose transport systems in Escherichia coli K-12." Kolodrubetz D., Schleif R. J. Bacteriol. 148:472-479(1981) [PubMed: 7028715] [Abstract] Cited for: FUNCTION IN ARABINOSE TRANSPORT, SUBCELLULAR LOCATION. Strain: K12.
[6]	"Genetic reconstitution of the high-affinity L-arabinose transport system." Horazdovsky B.F., Hogg R.W. J. Bacteriol. 171:3053-3059(1989) [PubMed: 2656640] [Abstract] Cited for: FUNCTION IN ARABINOSE TRANSPORT. Strain: BEK 180.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X06091 Genomic DNA. Translation: CAA29477.1. U00096 Genomic DNA. Translation: AAC74970.1. AP009048 Genomic DNA. Translation: BAA15720.1.
PIR	S01074.
RefSeq	AP_002518.1. NP_416413.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.A.1.2.2. ATP-binding cassette (ABC) superfamily.
Genome annotation databases
GeneID	946408.
GenomeReviews	Gene locus JW1888 in contig AP009048_GR. Gene locus b1900 in contig U00096_GR.
KEGG	ecj:JW1888. eco:b1900.
Organism-specific databases
EchoBASE	EB0056.
EcoGene	EG10058. araG.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AAF3.
OMA	P0AAF3. NADQHIR.
Enzyme and pathway databases
BioCyc	EcoCyc:ARAG-MON.
Family and domain databases
HAMAP	MF_01721. [Tree]
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017917. ABC_transptr_Ara_ATP-bd_AraG. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00005. ABC_tran. 2 hits. [Graphical view]
ProDom	PD000006. ABC_transporter. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 2 hits. PS51268. ARAG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARAG_ECOLI

Accession

Primary (citable) accession number: P0AAF3
Secondary accession number(s): P08531

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)