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Protein

Putrescine transporter PotE

Gene

potE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes both the uptake and excretion of putrescine. The uptake of putrescine is dependent on the membrane potential and the excretion involves putrescine-ornithine antiporter activity.UniRule annotation2 Publications

Enzyme regulationi

Uptake activity, but not antiporter activity, is inhibited by CCCP and N-ethylmaleimide (NEM). Uptake of putrescine is inhibited by high concentrations of ornithine.2 Publications

Kineticsi

  1. KM=1.8 µM for putrescine (for uptake activity)1 Publication
  2. KM=73 µM for putrescine (for antiporter activity)2 Publications
  3. KM=108 µM for ornithine (for antiporter activity)1 Publication
  1. Vmax=0.82 nmol/min/mg enzyme for antiporter activity1 Publication

pH dependencei

Optimum pH is 6.5 for uptake activity. Optimum pH is 9.2 for antiporter activity.1 Publication

GO - Molecular functioni

  • putrescine:ornithine antiporter activity Source: EcoCyc
  • symporter activity Source: EcoCyc

GO - Biological processi

  • ornithine transport Source: InterPro
  • putrescine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Antiport, Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:POTE-MONOMER.
ECOL316407:JW0679-MONOMER.
MetaCyc:POTE-MONOMER.

Protein family/group databases

TCDBi2.A.3.2.1. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine transporter PotEUniRule annotationCurated
Alternative name(s):
Putrescine-proton symporter / putrescine-ornithine antiporterUniRule annotationCurated
Gene namesi
Name:potE1 PublicationUniRule annotation
Ordered Locus Names:b0692, JW0679
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10753. potE.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9Cytoplasmic1 Publication9
Transmembranei10 – 30HelicalUniRule annotationAdd BLAST21
Topological domaini31 – 39Periplasmic1 Publication9
Transmembranei40 – 60HelicalUniRule annotationAdd BLAST21
Topological domaini61 – 90Cytoplasmic1 PublicationAdd BLAST30
Transmembranei91 – 111HelicalUniRule annotationAdd BLAST21
Topological domaini112 – 113Periplasmic1 Publication2
Transmembranei114 – 134HelicalUniRule annotationAdd BLAST21
Topological domaini135 – 151Cytoplasmic1 PublicationAdd BLAST17
Transmembranei152 – 172HelicalUniRule annotationAdd BLAST21
Topological domaini173 – 185Periplasmic1 PublicationAdd BLAST13
Transmembranei186 – 206HelicalUniRule annotationAdd BLAST21
Topological domaini207 – 224Cytoplasmic1 PublicationAdd BLAST18
Transmembranei225 – 245HelicalUniRule annotationAdd BLAST21
Topological domaini246 – 275Periplasmic1 PublicationAdd BLAST30
Transmembranei276 – 296HelicalUniRule annotationAdd BLAST21
Topological domaini297 – 320Cytoplasmic1 PublicationAdd BLAST24
Transmembranei321 – 341HelicalUniRule annotationAdd BLAST21
Topological domaini342 – 353Periplasmic1 PublicationAdd BLAST12
Transmembranei354 – 374HelicalUniRule annotationAdd BLAST21
Topological domaini375 – 386Cytoplasmic1 PublicationAdd BLAST12
Transmembranei387 – 407HelicalUniRule annotationAdd BLAST21
Topological domaini408 – 409Periplasmic1 Publication2
Transmembranei410 – 430HelicalUniRule annotationAdd BLAST21
Topological domaini431 – 439Cytoplasmic2 Publications9

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi62C → A or T: Strong decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi62C → S: Moderate decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi68K → A: Slight decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi77E → A, D, N or Q: Strong decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi78Y → L: Uptake activity decreases more than excretion activity. 1 Publication1
Mutagenesisi82K → A: Slight decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi90Y → L: Uptake activity decreases more than excretion activity. 1 Publication1
Mutagenesisi92Y → L: Moderate decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi201W → F, L or Y: Strong decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi207E → A, D, N or Q: Lack of both uptake and excretion activities. 1 Publication1
Mutagenesisi210C → A: Moderate decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi285C → A: Moderate decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi286C → A: Moderate decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi292W → F, L or Y: Strong decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi301K → A: Excretion activity decreases more than uptake activity. 1 Publication1
Mutagenesisi308Y → L: Excretion activity decreases more than uptake activity. 1 Publication1
Mutagenesisi422W → L: Uptake activity decreases more than excretion activity. 1 Publication1
Mutagenesisi425Y → F: Moderate decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi425Y → L: Strong decrease in both uptake and excretion activities. 1 Publication1
Mutagenesisi433E → A, D, N or Q: Strong decrease in both uptake and excretion activities. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542501 – 439Putrescine transporter PotEAdd BLAST439

Proteomic databases

PaxDbiP0AAF1.
PRIDEiP0AAF1.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4261131. 12 interactors.
IntActiP0AAF1. 1 interactor.
STRINGi511145.b0692.

Structurei

3D structure databases

ProteinModelPortaliP0AAF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amino acid-polyamine-organocation (APC) superfamily. Basic amino acid/polyamine antiporter (APA) (TC 2.A.3.2) family. [View classification]UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D5Q. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164507.
InParanoidiP0AAF1.
KOiK03756.
OMAiKHEPLAN.
PhylomeDBiP0AAF1.

Family and domain databases

HAMAPiMF_02073. Putrescine_transp. 1 hit.
InterProiIPR002293. AA/rel_permease1.
IPR004754. Amino_acid_antiprt.
IPR027566. Antiport_PotE.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00905. 2A0302. 1 hit.
TIGR04299. antiport_PotE. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AAF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQAKSNKMG VVQLTILTMV NMMGSGIIML PTKLAEVGTI SIISWLVTAV
60 70 80 90 100
GSMALAWAFA KCGMFSRKSG GMGGYAEYAF GKSGNFMANY TYGVSLLIAN
110 120 130 140 150
VAIAISAVGY GTELLGASLS PVQIGLATIG VLWICTVANF GGARITGQIS
160 170 180 190 200
SITVWGVIIP VVGLCIIGWF WFSPTLYVDS WNPHHAPFFS AVGSSIAMTL
210 220 230 240 250
WAFLGLESAC ANTDVVENPE RNVPIAVLGG TLGAAVIYIV STNVIAGIVP
260 270 280 290 300
NMELANSTAP FGLAFAQMFT PEVGKVIMAL MVMSCCGSLL GWQFTIAQVF
310 320 330 340 350
KSSSDEGYFP KIFSRVTKVD APVQGMLTIV IIQSGLALMT ISPSLNSQFN
360 370 380 390 400
VLVNLAVVTN IIPYILSMAA LVIIQKVANV PPSKAKVANF VAFVGAMYSF
410 420 430
YALYSSGEEA MLYGSIVTFL GWTLYGLVSP RFELKNKHG
Length:439
Mass (Da):46,495
Last modified:October 11, 2005 - v1
Checksum:iC6F800284DA8C5C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64495 Genomic DNA. Translation: AAA62786.1.
U00096 Genomic DNA. Translation: AAC73786.1.
AP009048 Genomic DNA. Translation: BAA35348.1.
PIRiB40839.
RefSeqiNP_415219.1. NC_000913.3.
WP_000075845.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73786; AAC73786; b0692.
BAA35348; BAA35348; BAA35348.
GeneIDi945422.
KEGGiecj:JW0679.
eco:b0692.
PATRICi32116579. VBIEscCol129921_0722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64495 Genomic DNA. Translation: AAA62786.1.
U00096 Genomic DNA. Translation: AAC73786.1.
AP009048 Genomic DNA. Translation: BAA35348.1.
PIRiB40839.
RefSeqiNP_415219.1. NC_000913.3.
WP_000075845.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAF1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261131. 12 interactors.
IntActiP0AAF1. 1 interactor.
STRINGi511145.b0692.

Protein family/group databases

TCDBi2.A.3.2.1. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbiP0AAF1.
PRIDEiP0AAF1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73786; AAC73786; b0692.
BAA35348; BAA35348; BAA35348.
GeneIDi945422.
KEGGiecj:JW0679.
eco:b0692.
PATRICi32116579. VBIEscCol129921_0722.

Organism-specific databases

EchoBASEiEB0746.
EcoGeneiEG10753. potE.

Phylogenomic databases

eggNOGiENOG4105D5Q. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164507.
InParanoidiP0AAF1.
KOiK03756.
OMAiKHEPLAN.
PhylomeDBiP0AAF1.

Enzyme and pathway databases

BioCyciEcoCyc:POTE-MONOMER.
ECOL316407:JW0679-MONOMER.
MetaCyc:POTE-MONOMER.

Miscellaneous databases

PROiP0AAF1.

Family and domain databases

HAMAPiMF_02073. Putrescine_transp. 1 hit.
InterProiIPR002293. AA/rel_permease1.
IPR004754. Amino_acid_antiprt.
IPR027566. Antiport_PotE.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00905. 2A0302. 1 hit.
TIGR04299. antiport_PotE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPOTE_ECOLI
AccessioniPrimary (citable) accession number: P0AAF1
Secondary accession number(s): P24170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.