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Protein

Putrescine-ornithine antiporter

Gene

potE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable putrescine-ornithine antiporter.

GO - Molecular functioni

  • putrescine:ornithine antiporter activity Source: EcoCyc
  • symporter activity Source: EcoCyc

GO - Biological processi

  • putrescine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Antiport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:POTE-MONOMER.
ECOL316407:JW0679-MONOMER.
MetaCyc:POTE-MONOMER.

Protein family/group databases

TCDBi2.A.3.2.1. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine-ornithine antiporter
Alternative name(s):
Putrescine transport protein
Gene namesi
Name:potE
Ordered Locus Names:b0692, JW0679
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10753. potE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010CytoplasmicSequence analysis
Transmembranei11 – 3121HelicalSequence analysisAdd
BLAST
Topological domaini32 – 376PeriplasmicSequence analysis
Transmembranei38 – 5821HelicalSequence analysisAdd
BLAST
Topological domaini59 – 8931CytoplasmicSequence analysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence analysisAdd
BLAST
Topological domaini111 – 1166PeriplasmicSequence analysis
Transmembranei117 – 13721HelicalSequence analysisAdd
BLAST
Topological domaini138 – 15114CytoplasmicSequence analysisAdd
BLAST
Transmembranei152 – 17221HelicalSequence analysisAdd
BLAST
Topological domaini173 – 18513PeriplasmicSequence analysisAdd
BLAST
Transmembranei186 – 20621HelicalSequence analysisAdd
BLAST
Topological domaini207 – 22317CytoplasmicSequence analysisAdd
BLAST
Transmembranei224 – 24421HelicalSequence analysisAdd
BLAST
Topological domaini245 – 27632PeriplasmicSequence analysisAdd
BLAST
Transmembranei277 – 29721HelicalSequence analysisAdd
BLAST
Topological domaini298 – 32427CytoplasmicSequence analysisAdd
BLAST
Transmembranei325 – 34521HelicalSequence analysisAdd
BLAST
Topological domaini346 – 3516PeriplasmicSequence analysis
Transmembranei352 – 37221HelicalSequence analysisAdd
BLAST
Topological domaini373 – 38614CytoplasmicSequence analysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence analysisAdd
BLAST
Topological domaini408 – 4092PeriplasmicSequence analysis
Transmembranei410 – 43021HelicalSequence analysisAdd
BLAST
Topological domaini431 – 4399CytoplasmicSequence analysis

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Putrescine-ornithine antiporterPRO_0000054250Add
BLAST

Proteomic databases

PaxDbiP0AAF1.

Expressioni

Inductioni

By low environmental pH.

Interactioni

Protein-protein interaction databases

BioGridi4261131. 12 interactions.
IntActiP0AAF1. 1 interaction.
STRINGi511145.b0692.

Structurei

3D structure databases

ProteinModelPortaliP0AAF1.
SMRiP0AAF1. Positions 4-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D5Q. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164507.
InParanoidiP0AAF1.
KOiK03756.
OMAiKHEPLAN.
OrthoDBiEOG6N945X.
PhylomeDBiP0AAF1.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004754. Amino_acid_antiprt.
IPR027566. Antiport_PotE.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00905. 2A0302. 1 hit.
TIGR04299. antiport_PotE. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AAF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQAKSNKMG VVQLTILTMV NMMGSGIIML PTKLAEVGTI SIISWLVTAV
60 70 80 90 100
GSMALAWAFA KCGMFSRKSG GMGGYAEYAF GKSGNFMANY TYGVSLLIAN
110 120 130 140 150
VAIAISAVGY GTELLGASLS PVQIGLATIG VLWICTVANF GGARITGQIS
160 170 180 190 200
SITVWGVIIP VVGLCIIGWF WFSPTLYVDS WNPHHAPFFS AVGSSIAMTL
210 220 230 240 250
WAFLGLESAC ANTDVVENPE RNVPIAVLGG TLGAAVIYIV STNVIAGIVP
260 270 280 290 300
NMELANSTAP FGLAFAQMFT PEVGKVIMAL MVMSCCGSLL GWQFTIAQVF
310 320 330 340 350
KSSSDEGYFP KIFSRVTKVD APVQGMLTIV IIQSGLALMT ISPSLNSQFN
360 370 380 390 400
VLVNLAVVTN IIPYILSMAA LVIIQKVANV PPSKAKVANF VAFVGAMYSF
410 420 430
YALYSSGEEA MLYGSIVTFL GWTLYGLVSP RFELKNKHG
Length:439
Mass (Da):46,495
Last modified:October 11, 2005 - v1
Checksum:iC6F800284DA8C5C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64495 Genomic DNA. Translation: AAA62786.1.
U00096 Genomic DNA. Translation: AAC73786.1.
AP009048 Genomic DNA. Translation: BAA35348.1.
PIRiB40839.
RefSeqiNP_415219.1. NC_000913.3.
WP_000075845.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73786; AAC73786; b0692.
BAA35348; BAA35348; BAA35348.
GeneIDi945422.
KEGGiecj:JW0679.
eco:b0692.
PATRICi32116579. VBIEscCol129921_0722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64495 Genomic DNA. Translation: AAA62786.1.
U00096 Genomic DNA. Translation: AAC73786.1.
AP009048 Genomic DNA. Translation: BAA35348.1.
PIRiB40839.
RefSeqiNP_415219.1. NC_000913.3.
WP_000075845.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAF1.
SMRiP0AAF1. Positions 4-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261131. 12 interactions.
IntActiP0AAF1. 1 interaction.
STRINGi511145.b0692.

Protein family/group databases

TCDBi2.A.3.2.1. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbiP0AAF1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73786; AAC73786; b0692.
BAA35348; BAA35348; BAA35348.
GeneIDi945422.
KEGGiecj:JW0679.
eco:b0692.
PATRICi32116579. VBIEscCol129921_0722.

Organism-specific databases

EchoBASEiEB0746.
EcoGeneiEG10753. potE.

Phylogenomic databases

eggNOGiENOG4105D5Q. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164507.
InParanoidiP0AAF1.
KOiK03756.
OMAiKHEPLAN.
OrthoDBiEOG6N945X.
PhylomeDBiP0AAF1.

Enzyme and pathway databases

BioCyciEcoCyc:POTE-MONOMER.
ECOL316407:JW0679-MONOMER.
MetaCyc:POTE-MONOMER.

Miscellaneous databases

PROiP0AAF1.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004754. Amino_acid_antiprt.
IPR027566. Antiport_PotE.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00905. 2A0302. 1 hit.
TIGR04299. antiport_PotE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Coexistence of the genes for putrescine transport protein and ornithine decarboxylase at 16 min on Escherichia coli chromosome."
    Kashiwagi K., Suzuki T., Suzuki F., Furuchi T., Kobayashi H., Igarashi K.
    J. Biol. Chem. 266:20922-20927(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPOTE_ECOLI
AccessioniPrimary (citable) accession number: P0AAF1
Secondary accession number(s): P24170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: July 6, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.