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Protein

D-serine/D-alanine/glycine transporter

Gene

cycA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Permease that is involved in the transport across the cytoplasmic membrane of D-alanine, D-serine and glycine.

GO - Molecular functioni

  • antibiotic transporter activity Source: EcoCyc
  • antiporter activity Source: GO_Central
  • beta-alanine transmembrane transporter activity Source: EcoCyc
  • D-alanine transmembrane transporter activity Source: EcoCyc
  • D-serine transmembrane transporter activity Source: EcoCyc
  • glycine transmembrane transporter activity Source: EcoCyc
  • L-alanine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • antibiotic transport Source: EcoCyc
  • beta-alanine transport Source: EcoCyc
  • D-alanine transport Source: EcoCyc
  • D-serine transport Source: EcoCyc
  • glycine transport Source: EcoCyc
  • L-alanine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CYCA-MONOMER.
ECOL316407:JW4166-MONOMER.
MetaCyc:CYCA-MONOMER.

Protein family/group databases

TCDBi2.A.3.1.7. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
D-serine/D-alanine/glycine transporter
Gene namesi
Name:cycA
Synonyms:dagA, ytfD
Ordered Locus Names:b4208, JW4166
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12504. cycA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2929CytoplasmicSequence analysisAdd
BLAST
Transmembranei30 – 5021HelicalSequence analysisAdd
BLAST
Transmembranei51 – 7121HelicalSequence analysisAdd
BLAST
Topological domaini72 – 10130CytoplasmicSequence analysisAdd
BLAST
Transmembranei102 – 12221HelicalSequence analysisAdd
BLAST
Topological domaini123 – 13614PeriplasmicSequence analysisAdd
BLAST
Transmembranei137 – 15721HelicalSequence analysisAdd
BLAST
Topological domaini158 – 1614CytoplasmicSequence analysis
Transmembranei162 – 18221HelicalSequence analysisAdd
BLAST
Topological domaini183 – 21028PeriplasmicSequence analysisAdd
BLAST
Transmembranei211 – 23121HelicalSequence analysisAdd
BLAST
Topological domaini232 – 25524CytoplasmicSequence analysisAdd
BLAST
Transmembranei256 – 27621HelicalSequence analysisAdd
BLAST
Topological domaini277 – 2826PeriplasmicSequence analysis
Transmembranei283 – 30321HelicalSequence analysisAdd
BLAST
Topological domaini304 – 34946CytoplasmicSequence analysisAdd
BLAST
Transmembranei350 – 37021HelicalSequence analysisAdd
BLAST
Transmembranei371 – 39121HelicalSequence analysisAdd
BLAST
Topological domaini392 – 41221CytoplasmicSequence analysisAdd
BLAST
Transmembranei413 – 43321HelicalSequence analysisAdd
BLAST
Topological domaini434 – 4407PeriplasmicSequence analysis
Transmembranei441 – 46121HelicalSequence analysisAdd
BLAST
Topological domaini462 – 4709CytoplasmicSequence analysis

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 470470D-serine/D-alanine/glycine transporterPRO_0000054199Add
BLAST

Proteomic databases

EPDiP0AAE0.
PaxDbiP0AAE0.
PRIDEiP0AAE0.

Interactioni

Protein-protein interaction databases

BioGridi4262718. 170 interactions.
DIPiDIP-9359N.
IntActiP0AAE0. 1 interaction.
MINTiMINT-1286142.
STRINGi511145.b4208.

Structurei

3D structure databases

ProteinModelPortaliP0AAE0.
SMRiP0AAE0. Positions 28-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C85. Bacteria.
COG1113. LUCA.
HOGENOMiHOG000261849.
InParanoidiP0AAE0.
KOiK11737.
OMAiLCWIITG.
OrthoDBiEOG6HQSQM.
PhylomeDBiP0AAE0.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDQVKVVAD DQAPAEQSLR RNLTNRHIQL IAIGGAIGTG LFMGSGKTIS
60 70 80 90 100
LAGPSIIFVY MIIGFMLFFV MRAMGELLLS NLEYKSFSDF ASDLLGPWAG
110 120 130 140 150
YFTGWTYWFC WVVTGMADVV AITAYAQFWF PDLSDWVASL AVIVLLLTLN
160 170 180 190 200
LATVKMFGEM EFWFAMIKIV AIVSLIVVGL VMVAMHFQSP TGVEASFAHL
210 220 230 240 250
WNDGGWFPKG LSGFFAGFQI AVFAFVGIEL VGTTAAETKD PEKSLPRAIN
260 270 280 290 300
SIPIRIIMFY VFALIVIMSV TPWSSVVPEK SPFVELFVLV GLPAAASVIN
310 320 330 340 350
FVVLTSAASS ANSGVFSTSR MLFGLAQEGV APKAFAKLSK RAVPAKGLTF
360 370 380 390 400
SCICLLGGVV MLYVNPSVIG AFTMITTVSA ILFMFVWTII LCSYLVYRKQ
410 420 430 440 450
RPHLHEKSIY KMPLGKLMCW VCMAFFVFVV VLLTLEDDTR QALLVTPLWF
460 470
IALGLGWLFI GKKRAAELRK
Length:470
Mass (Da):51,660
Last modified:October 11, 2005 - v1
Checksum:iF9279E48D838EAF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97104.1.
U00096 Genomic DNA. Translation: AAC77165.1.
AP009048 Genomic DNA. Translation: BAE78209.1.
PIRiS56433.
RefSeqiNP_418629.1. NC_000913.3.
WP_000228346.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77165; AAC77165; b4208.
BAE78209; BAE78209; BAE78209.
GeneIDi948725.
KEGGiecj:JW4166.
eco:b4208.
PATRICi32123991. VBIEscCol129921_4340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97104.1.
U00096 Genomic DNA. Translation: AAC77165.1.
AP009048 Genomic DNA. Translation: BAE78209.1.
PIRiS56433.
RefSeqiNP_418629.1. NC_000913.3.
WP_000228346.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAE0.
SMRiP0AAE0. Positions 28-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262718. 170 interactions.
DIPiDIP-9359N.
IntActiP0AAE0. 1 interaction.
MINTiMINT-1286142.
STRINGi511145.b4208.

Protein family/group databases

TCDBi2.A.3.1.7. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

EPDiP0AAE0.
PaxDbiP0AAE0.
PRIDEiP0AAE0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77165; AAC77165; b4208.
BAE78209; BAE78209; BAE78209.
GeneIDi948725.
KEGGiecj:JW4166.
eco:b4208.
PATRICi32123991. VBIEscCol129921_4340.

Organism-specific databases

EchoBASEiEB2397.
EcoGeneiEG12504. cycA.

Phylogenomic databases

eggNOGiENOG4105C85. Bacteria.
COG1113. LUCA.
HOGENOMiHOG000261849.
InParanoidiP0AAE0.
KOiK11737.
OMAiLCWIITG.
OrthoDBiEOG6HQSQM.
PhylomeDBiP0AAE0.

Enzyme and pathway databases

BioCyciEcoCyc:CYCA-MONOMER.
ECOL316407:JW4166-MONOMER.
MetaCyc:CYCA-MONOMER.

Miscellaneous databases

PROiP0AAE0.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Berlyn M., Rudd K.E.
    Unpublished observations (JUL-1995)
    Cited for: IDENTIFICATION AS CYCA.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCYCA_ECOLI
AccessioniPrimary (citable) accession number: P0AAE0
Secondary accession number(s): P39312, Q2M697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: June 8, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.