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P0AAE0 (CYCA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-serine/D-alanine/glycine transporter
Gene names
Name:cycA
Synonyms:dagA, ytfD
Ordered Locus Names:b4208, JW4166
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Permease that is involved in the transport across the cytoplasmic membrane of D-alanine, D-serine and glycine.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family. [View classification]

Ontologies

Keywords
   Biological processAmino-acid transport
Transport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionamino acid transmembrane transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 470470D-serine/D-alanine/glycine transporter
PRO_0000054199

Regions

Topological domain1 – 2929Cytoplasmic Potential
Transmembrane30 – 5021Helical; Potential
Transmembrane51 – 7121Helical; Potential
Topological domain72 – 10130Cytoplasmic Potential
Transmembrane102 – 12221Helical; Potential
Topological domain123 – 13614Periplasmic Potential
Transmembrane137 – 15721Helical; Potential
Topological domain158 – 1614Cytoplasmic Potential
Transmembrane162 – 18221Helical; Potential
Topological domain183 – 21028Periplasmic Potential
Transmembrane211 – 23121Helical; Potential
Topological domain232 – 25524Cytoplasmic Potential
Transmembrane256 – 27621Helical; Potential
Topological domain277 – 2826Periplasmic Potential
Transmembrane283 – 30321Helical; Potential
Topological domain304 – 34946Cytoplasmic Potential
Transmembrane350 – 37021Helical; Potential
Transmembrane371 – 39121Helical; Potential
Topological domain392 – 41221Cytoplasmic Potential
Transmembrane413 – 43321Helical; Potential
Topological domain434 – 4407Periplasmic Potential
Transmembrane441 – 46121Helical; Potential
Topological domain462 – 4709Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P0AAE0 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: F9279E48D838EAF8

FASTA47051,660
        10         20         30         40         50         60 
MVDQVKVVAD DQAPAEQSLR RNLTNRHIQL IAIGGAIGTG LFMGSGKTIS LAGPSIIFVY 

        70         80         90        100        110        120 
MIIGFMLFFV MRAMGELLLS NLEYKSFSDF ASDLLGPWAG YFTGWTYWFC WVVTGMADVV 

       130        140        150        160        170        180 
AITAYAQFWF PDLSDWVASL AVIVLLLTLN LATVKMFGEM EFWFAMIKIV AIVSLIVVGL 

       190        200        210        220        230        240 
VMVAMHFQSP TGVEASFAHL WNDGGWFPKG LSGFFAGFQI AVFAFVGIEL VGTTAAETKD 

       250        260        270        280        290        300 
PEKSLPRAIN SIPIRIIMFY VFALIVIMSV TPWSSVVPEK SPFVELFVLV GLPAAASVIN 

       310        320        330        340        350        360 
FVVLTSAASS ANSGVFSTSR MLFGLAQEGV APKAFAKLSK RAVPAKGLTF SCICLLGGVV 

       370        380        390        400        410        420 
MLYVNPSVIG AFTMITTVSA ILFMFVWTII LCSYLVYRKQ RPHLHEKSIY KMPLGKLMCW 

       430        440        450        460        470 
VCMAFFVFVV VLLTLEDDTR QALLVTPLWF IALGLGWLFI GKKRAAELRK

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]Berlyn M., Rudd K.E.
Unpublished observations (JUL-1995)
Cited for: IDENTIFICATION AS CYCA.
[5]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U14003 Genomic DNA. Translation: AAA97104.1.
U00096 Genomic DNA. Translation: AAC77165.1.
AP009048 Genomic DNA. Translation: BAE78209.1.
PIRS56433.
RefSeqNP_418629.1. NC_000913.2.

3D structure databases

ProteinModelPortalP0AAE0.
ModBaseSearch...

Protein-protein interaction databases

IntActP0AAE0. 1 interaction.
MINTMINT-1286142.

Protein family/group databases

TCDB2.A.3.1.7. amino acid-polyamine-organocation (APC) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000003545; EBESCP00000003545; EBESCG00000002896.
EBESCT00000014964; EBESCP00000014255; EBESCG00000014024.
GeneID948725.
GenomeReviewsGene locus JW4166 in contig AP009048_GR.
Gene locus b4208 in contig U00096_GR.
KEGGecj:JW4166.
eco:b4208.
PATRIC32123991. VBIEscCol129921_4340.

Organism-specific databases

EchoBASEEB2397.
EcoGeneEG12504. cycA.

Phylogenomic databases

eggNOGCOG1113.
GeneTreeEBGT00050000009004.
HOGENOMHBG492579.
OMALMVTPLW.
PhylomeDBP0AAE0.
ProtClustDBPRK11049.

Enzyme and pathway databases

BioCycEcoCyc:CYCA-MONOMER.

Gene expression databases

GenevestigatorP0AAE0.

Family and domain databases

InterProIPR004841. AA-permease_dom.
IPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
KOK11737.
PANTHERPTHR11785. AA/rel_permease1. 1 hit.
PfamPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
PROSITEPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYCA_ECOLI
AccessionPrimary (citable) accession number: P0AAE0
Secondary accession number(s): P39312, Q2M697
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

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