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Protein

Iron-binding protein IscA

Gene

iscA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is able to transfer iron-sulfur clusters to apo-ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that IscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transient iron-sulfur cluster in IscU in the presence of IscS, L-cysteine and the thioredoxin reductase system TrxA/TrxB.

Cofactori

Fe cationNote: Binds 2 iron ions per dimer. The dimer may bind additional iron ions.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi35 – 351IronCurated
Metal bindingi99 – 991IronCurated
Metal bindingi101 – 1011IronCurated

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: UniProtKB
  • 4 iron, 4 sulfur cluster binding Source: GO_Central
  • ferrous iron binding Source: EcoCyc
  • iron chaperone activity Source: EcoCyc
  • structural molecule activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12132-MONOMER.
ECOL316407:JW2512-MONOMER.
MetaCyc:EG12132-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-binding protein IscA
Alternative name(s):
Iron-sulfur cluster assembly protein
Gene namesi
Name:iscA
Synonyms:yfhF
Ordered Locus Names:b2528, JW2512
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12132. iscA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351C → S: Decrease of iron binding activity. 1 Publication
Mutagenesisi99 – 991C → S: Loss of iron binding activity. 1 Publication
Mutagenesisi101 – 1011C → S: Loss of iron binding activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 107107Iron-binding protein IscAPRO_0000076997Add
BLAST

Proteomic databases

EPDiP0AAC8.
PaxDbiP0AAC8.
PRIDEiP0AAC8.

Expressioni

Inductioni

Repressed by IscR.

Interactioni

Subunit structurei

Homodimer; may form tetramers and higher multimers.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
fdxP0A9R44EBI-767026,EBI-767037

Protein-protein interaction databases

BioGridi4260603. 23 interactions.
DIPiDIP-35854N.
IntActiP0AAC8. 5 interactions.
STRINGi511145.b2528.

Structurei

Secondary structure

1
107
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 2014Combined sources
Beta strandi24 – 329Combined sources
Beta strandi34 – 363Combined sources
Beta strandi38 – 4710Combined sources
Beta strandi52 – 576Combined sources
Beta strandi60 – 656Combined sources
Helixi66 – 683Combined sources
Helixi69 – 724Combined sources
Beta strandi76 – 827Combined sources
Beta strandi85 – 917Combined sources
Beta strandi93 – 953Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R94X-ray2.30A/B1-105[»]
1R95X-ray2.65A/B1-105[»]
1S98X-ray2.30A/B1-107[»]
ProteinModelPortaliP0AAC8.
SMRiP0AAC8. Positions 1-97.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AAC8.

Family & Domainsi

Sequence similaritiesi

Belongs to the HesB/IscA family.Curated

Phylogenomic databases

eggNOGiENOG4108Z4V. Bacteria.
COG0316. LUCA.
HOGENOMiHOG000228314.
InParanoidiP0AAC8.
KOiK13628.
OMAiEYTMEYV.
PhylomeDBiP0AAC8.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
HAMAPiMF_01429. Fe_S_insert_IscA. 1 hit.
InterProiIPR000361. FeS_biogenesis.
IPR016092. FeS_cluster_insertion.
IPR017870. FeS_cluster_insertion_CS.
IPR031108. ISCA-like.
IPR011302. IscA_proteobacteria.
[Graphical view]
PANTHERiPTHR10072:SF48. PTHR10072:SF48. 1 hit.
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
TIGRFAMsiTIGR02011. IscA. 1 hit.
TIGR00049. TIGR00049. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSITLSDSAA ARVNTFLANR GKGFGLRLGV RTSGCSGMAY VLEFVDEPTP
60 70 80 90 100
EDIVFEDKGV KVVVDGKSLQ FLDGTQLDFV KEGLNEGFKF TNPNVKDECG

CGESFHV
Length:107
Mass (Da):11,556
Last modified:September 13, 2005 - v1
Checksum:iCBA945AD547E77DD
GO

Sequence cautioni

The sequence U01827 differs from that shown. Reason: Frameshift at several positions. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01827 Unassigned DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75581.1.
AP009048 Genomic DNA. Translation: BAA16422.1.
PIRiG65029.
RefSeqiNP_417023.1. NC_000913.3.
WP_000028953.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75581; AAC75581; b2528.
BAA16422; BAA16422; BAA16422.
GeneIDi946999.
KEGGiecj:JW2512.
eco:b2528.
PATRICi32120453. VBIEscCol129921_2629.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01827 Unassigned DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75581.1.
AP009048 Genomic DNA. Translation: BAA16422.1.
PIRiG65029.
RefSeqiNP_417023.1. NC_000913.3.
WP_000028953.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R94X-ray2.30A/B1-105[»]
1R95X-ray2.65A/B1-105[»]
1S98X-ray2.30A/B1-107[»]
ProteinModelPortaliP0AAC8.
SMRiP0AAC8. Positions 1-97.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260603. 23 interactions.
DIPiDIP-35854N.
IntActiP0AAC8. 5 interactions.
STRINGi511145.b2528.

Proteomic databases

EPDiP0AAC8.
PaxDbiP0AAC8.
PRIDEiP0AAC8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75581; AAC75581; b2528.
BAA16422; BAA16422; BAA16422.
GeneIDi946999.
KEGGiecj:JW2512.
eco:b2528.
PATRICi32120453. VBIEscCol129921_2629.

Organism-specific databases

EchoBASEiEB2053.
EcoGeneiEG12132. iscA.

Phylogenomic databases

eggNOGiENOG4108Z4V. Bacteria.
COG0316. LUCA.
HOGENOMiHOG000228314.
InParanoidiP0AAC8.
KOiK13628.
OMAiEYTMEYV.
PhylomeDBiP0AAC8.

Enzyme and pathway databases

BioCyciEcoCyc:EG12132-MONOMER.
ECOL316407:JW2512-MONOMER.
MetaCyc:EG12132-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AAC8.
PROiP0AAC8.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
HAMAPiMF_01429. Fe_S_insert_IscA. 1 hit.
InterProiIPR000361. FeS_biogenesis.
IPR016092. FeS_cluster_insertion.
IPR017870. FeS_cluster_insertion_CS.
IPR031108. ISCA-like.
IPR011302. IscA_proteobacteria.
[Graphical view]
PANTHERiPTHR10072:SF48. PTHR10072:SF48. 1 hit.
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
TIGRFAMsiTIGR02011. IscA. 1 hit.
TIGR00049. TIGR00049. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISCA_ECOLI
AccessioniPrimary (citable) accession number: P0AAC8
Secondary accession number(s): P36539, P77691
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

2 iron atoms may bind between dimers.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.