Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Modulator of FtsH protease YccA

Gene

yccA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negatively modulates the activity of the FtsH protease for membrane substrates. Overexpression or stabilizing YccA counteracts the FtsH-mediated degradation of SecY when the SecYEG preprotein translocator is jammed.1 Publication

GO - Biological processi

  • negative regulation of apoptotic process Source: InterPro
  • regulation of proteolysis Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG11113-MONOMER.
ECOL316407:JW0953-MONOMER.

Protein family/group databases

TCDBi1.A.14.2.1. the testis-enhanced gene transfer (tegt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Modulator of FtsH protease YccA
Gene namesi
Name:yccA
Ordered Locus Names:b0970, JW0953
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11113. yccA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicCuratedAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 46ExtracellularCurated3
Transmembranei47 – 64HelicalSequence analysisAdd BLAST18
Topological domaini65 – 73CytoplasmicCurated9
Transmembranei74 – 94HelicalSequence analysisAdd BLAST21
Topological domaini95 – 104ExtracellularCurated10
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Topological domaini126 – 132CytoplasmicCurated7
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Topological domaini154 – 157ExtracellularCurated4
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Topological domaini179 – 195CytoplasmicCuratedAdd BLAST17
Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
Topological domaini217 – 219Extracellular3

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No effect on SecY degradation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 12MDRIV…SHDRT → MTMITPSLHDRI: Protein is as sensitive to FtsH degradation as the wild-type. 1 PublicationAdd BLAST12
Mutagenesisi5 – 12Missing in yccA11; increases the half-life of overexpressed SecY, F(0) ATP synthase subunit a and itself in an HflKC-dependent fashion, but has no effect on degradation of cytosolic substrates sigma-32 or the phage lambda cII protein. Partially dominant over the wild-type allele. This protein interacts with but is not degraded by FtsH. 1 Publication8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001791021 – 219Modulator of FtsH protease YccAAdd BLAST219

Post-translational modificationi

YccA is processively degraded by FtsH; degradation initiates via the approximately 20 residue N-terminal tail in a sequence non-specific manner. The deletion mutant yccA11 is resistant to FtsH-mediated degradation, probably because it is too short to be degraded by FtsH.

Proteomic databases

PaxDbiP0AAC6.
PRIDEiP0AAC6.

Expressioni

Inductioni

Regulated in a Cpx-dependent fashion.1 Publication

Interactioni

Subunit structurei

Both wild-type and the yccA11 mutant interact with the FtsH/HflKC complex.

Protein-protein interaction databases

BioGridi4260037. 11 interactors.
DIPiDIP-47918N.
IntActiP0AAC6. 1 interactor.
MINTiMINT-1274885.
STRINGi511145.b0970.

Structurei

3D structure databases

ProteinModelPortaliP0AAC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BI1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108MN8. Bacteria.
COG0670. LUCA.
HOGENOMiHOG000060391.
InParanoidiP0AAC6.
KOiK19416.
OMAiMAMNLPR.
PhylomeDBiP0AAC6.

Family and domain databases

InterProiIPR006213. Bax_inhbtr1_CS.
IPR006214. Bax_inhibitor_1-related.
[Graphical view]
PANTHERiPTHR23291. PTHR23291. 1 hit.
PROSITEiPS01243. BI1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRIVSSSHD RTSLLSTHKV LRNTYFLLSL TLAFSAITAT ASTVLMLPSP
60 70 80 90 100
GLILTLVGMY GLMFLTYKTA NKPTGIISAF AFTGFLGYIL GPILNTYLSA
110 120 130 140 150
GMGDVIAMAL GGTALVFFCC SAYVLTTRKD MSFLGGMLMA GIVVVLIGMV
160 170 180 190 200
ANIFLQLPAL HLAISAVFIL ISSGAILFET SNIIHGGETN YIRATVSLYV
210
SLYNIFVSLL SILGFASRD
Length:219
Mass (Da):23,363
Last modified:April 1, 1988 - v1
Checksum:i4F787B853042ACD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00547 Genomic DNA. Translation: CAA25218.1.
U00096 Genomic DNA. Translation: AAC74056.1.
AP009048 Genomic DNA. Translation: BAA35735.1.
PIRiS07180.
RefSeqiNP_415490.1. NC_000913.3.
WP_000375136.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74056; AAC74056; b0970.
BAA35735; BAA35735; BAA35735.
GeneIDi946016.
KEGGiecj:JW0953.
eco:b0970.
PATRICi32117161. VBIEscCol129921_1005.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00547 Genomic DNA. Translation: CAA25218.1.
U00096 Genomic DNA. Translation: AAC74056.1.
AP009048 Genomic DNA. Translation: BAA35735.1.
PIRiS07180.
RefSeqiNP_415490.1. NC_000913.3.
WP_000375136.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAC6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260037. 11 interactors.
DIPiDIP-47918N.
IntActiP0AAC6. 1 interactor.
MINTiMINT-1274885.
STRINGi511145.b0970.

Protein family/group databases

TCDBi1.A.14.2.1. the testis-enhanced gene transfer (tegt) family.

Proteomic databases

PaxDbiP0AAC6.
PRIDEiP0AAC6.

Protocols and materials databases

DNASUi946016.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74056; AAC74056; b0970.
BAA35735; BAA35735; BAA35735.
GeneIDi946016.
KEGGiecj:JW0953.
eco:b0970.
PATRICi32117161. VBIEscCol129921_1005.

Organism-specific databases

EchoBASEiEB1104.
EcoGeneiEG11113. yccA.

Phylogenomic databases

eggNOGiENOG4108MN8. Bacteria.
COG0670. LUCA.
HOGENOMiHOG000060391.
InParanoidiP0AAC6.
KOiK19416.
OMAiMAMNLPR.
PhylomeDBiP0AAC6.

Enzyme and pathway databases

BioCyciEcoCyc:EG11113-MONOMER.
ECOL316407:JW0953-MONOMER.

Miscellaneous databases

PROiP0AAC6.

Family and domain databases

InterProiIPR006213. Bax_inhbtr1_CS.
IPR006214. Bax_inhibitor_1-related.
[Graphical view]
PANTHERiPTHR23291. PTHR23291. 1 hit.
PROSITEiPS01243. BI1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYCCA_ECOLI
AccessioniPrimary (citable) accession number: P0AAC6
Secondary accession number(s): P06967
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.