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Protein

Universal stress protein E

Gene

uspE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for resistance to DNA-damaging agents.1 Publication

GO - Biological processi

  • bacterial-type flagellum-dependent cell motility Source: EcoCyc
  • cellular response to hydrogen peroxide Source: EcoCyc
  • cellular response to UV Source: EcoCyc
  • single-species biofilm formation Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG11246-MONOMER.
ECOL316407:JW1327-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Universal stress protein E
Gene namesi
Name:uspE
Synonyms:ydaA
Ordered Locus Names:b1333, JW1327
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11246. uspE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 316315Universal stress protein EPRO_0000147417Add
BLAST

Proteomic databases

EPDiP0AAC0.
PaxDbiP0AAC0.
PRIDEiP0AAC0.

2D gel databases

SWISS-2DPAGEP0AAC0.

Expressioni

Inductioni

During growth inhibition caused by the exhaustion of any of a variety of nutrients (carbon, nitrogen, phosphate, sulfate, required amino acid) or by the presence of a variety of toxic agents. Positively regulated by guanosine 3',5'-bisphosphate (ppGpp) and by a RecA/FtsK-dependent regulatory pathway.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260143. 13 interactions.
DIPiDIP-48118N.
IntActiP0AAC0. 1 interaction.
MINTiMINT-7290918.
STRINGi511145.b1333.

Structurei

Secondary structure

1
316
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105Combined sources
Helixi19 – 2810Combined sources
Beta strandi34 – 418Combined sources
Helixi44 – 463Combined sources
Turni49 – 513Combined sources
Helixi54 – 8229Combined sources
Beta strandi86 – 927Combined sources
Helixi96 – 10712Combined sources
Beta strandi110 – 1178Combined sources
Helixi129 – 1379Combined sources
Beta strandi142 – 1487Combined sources
Beta strandi156 – 1605Combined sources
Helixi169 – 18416Combined sources
Turni185 – 1873Combined sources
Beta strandi192 – 1987Combined sources
Helixi215 – 23622Combined sources
Beta strandi243 – 2486Combined sources
Helixi250 – 26011Combined sources
Beta strandi264 – 2685Combined sources
Helixi283 – 2908Combined sources
Beta strandi294 – 2985Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5CB0X-ray3.21A/B1-316[»]
ProteinModelPortaliP0AAC0.
SMRiP0AAC0. Positions 1-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the universal stress protein A family.Curated

Phylogenomic databases

eggNOGiENOG4105Q9Y. Bacteria.
COG0589. LUCA.
HOGENOMiHOG000277987.
InParanoidiP0AAC0.
KOiK14055.
OMAiMAKYQNM.
OrthoDBiEOG6SBT15.
PhylomeDBiP0AAC0.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR006016. UspA.
[Graphical view]
PfamiPF00582. Usp. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMYQNMLVV IDPNQDDQPA LRRAVYLHQR IGGKIKAFLP IYDFSYEMTT
60 70 80 90 100
LLSPDERTAM RQGVISQRTA WIHEQAKYYL NAGVPIEIKV VWHNRPFEAI
110 120 130 140 150
IQEVISGGHD LVLKMAHQHD RLEAVIFTPT DWHLLRKCPS PVWMVKDQPW
160 170 180 190 200
PEGGKALVAV NLASEEPYHN ALNEKLVKET IELAEQVNHT EVHLVGAYPV
210 220 230 240 250
TPINIAIELP EFDPSVYNDA IRGQHLLAMK ALRQKFGINE NMTHVEKGLP
260 270 280 290 300
EEVIPDLAEH LQAGIVVLGT VGRTGISAAF LGNTAEQVID HLRCDLLVIK
310
PDQYQTPVEL DDEEDD
Length:316
Mass (Da):35,707
Last modified:January 23, 2007 - v2
Checksum:iF5F416848D452378
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551Missing in AAA87982 (PubMed:6292868).Curated
Sequence conflicti59 – 7315AMRQG…TAWIH → RYASGRHQPAYSLDP (PubMed:6292868).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74415.1.
AP009048 Genomic DNA. Translation: BAA14926.1.
J01608 Genomic DNA. Translation: AAA87982.1.
PIRiH64882. QQECX.
RefSeqiNP_415849.1. NC_000913.3.
WP_001262123.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74415; AAC74415; b1333.
BAA14926; BAA14926; BAA14926.
GeneIDi945904.
KEGGiecj:JW1327.
eco:b1333.
PATRICi32117942. VBIEscCol129921_1391.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74415.1.
AP009048 Genomic DNA. Translation: BAA14926.1.
J01608 Genomic DNA. Translation: AAA87982.1.
PIRiH64882. QQECX.
RefSeqiNP_415849.1. NC_000913.3.
WP_001262123.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5CB0X-ray3.21A/B1-316[»]
ProteinModelPortaliP0AAC0.
SMRiP0AAC0. Positions 1-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260143. 13 interactions.
DIPiDIP-48118N.
IntActiP0AAC0. 1 interaction.
MINTiMINT-7290918.
STRINGi511145.b1333.

2D gel databases

SWISS-2DPAGEP0AAC0.

Proteomic databases

EPDiP0AAC0.
PaxDbiP0AAC0.
PRIDEiP0AAC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74415; AAC74415; b1333.
BAA14926; BAA14926; BAA14926.
GeneIDi945904.
KEGGiecj:JW1327.
eco:b1333.
PATRICi32117942. VBIEscCol129921_1391.

Organism-specific databases

EchoBASEiEB1227.
EcoGeneiEG11246. uspE.

Phylogenomic databases

eggNOGiENOG4105Q9Y. Bacteria.
COG0589. LUCA.
HOGENOMiHOG000277987.
InParanoidiP0AAC0.
KOiK14055.
OMAiMAKYQNM.
OrthoDBiEOG6SBT15.
PhylomeDBiP0AAC0.

Enzyme and pathway databases

BioCyciEcoCyc:EG11246-MONOMER.
ECOL316407:JW1327-MONOMER.

Miscellaneous databases

PROiP0AAC0.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR006016. UspA.
[Graphical view]
PfamiPF00582. Usp. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Nucleotide sequence of the fnr gene and primary structure of the Enr protein of Escherichia coli."
    Shaw D.J., Guest J.R.
    Nucleic Acids Res. 10:6119-6130(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
  5. Cited for: PROTEIN SEQUENCE OF 2-5.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
    Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
    Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: B / BL21.
  7. "The universal stress protein paralogues of Escherichia coli are co-ordinately regulated and co-operate in the defence against DNA damage."
    Gustavsson N., Diez A., Nystroem T.
    Mol. Microbiol. 43:107-117(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENE NAME, TRANSCRIPTIONAL REGULATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiUSPE_ECOLI
AccessioniPrimary (citable) accession number: P0AAC0
Secondary accession number(s): P03807, P77421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.