P0AAC0 (USPE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Universal stress protein E

Gene names

Name:	uspE
Synonyms:	ydaA
Ordered Locus Names:	b1333, JW1327

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for resistance to DNA-damaging agents. Ref.7
Subcellular location	Cytoplasm By similarity.
Induction	During growth inhibition caused by the exhaustion of any of a variety of nutrients (carbon, nitrogen, phosphate, sulfate, required amino acid) or by the presence of a variety of toxic agents. Positively regulated by guanosine 3',5'-bisphosphate (ppGpp) and by a RecA/FtsK-dependent regulatory pathway. Ref.7
Sequence similarities	Belongs to the universal stress protein A family.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular response to UV Inferred from mutant phenotype Ref.7. Source: EcoCyc cellular response to hydrogen peroxide Inferred from expression pattern. Source: EcoCyc flagellar cell motility Inferred from mutant phenotype. Source: EcoCyc single-species biofilm formation Inferred from mutant phenotype. Source: EcoCyc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 316	315	Universal stress protein E		PRO_0000147417
Experimental info
	Sequence conflict	55	1	Missing in AAA87982. Ref.4
	Sequence conflict	59 – 73	15	AMRQG…TAWIH → RYASGRHQPAYSLDP Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P0AAC0 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F5F416848D452378	FASTA	316	35,707
	10 20 30 40 50 60 MAMYQNMLVV IDPNQDDQPA LRRAVYLHQR IGGKIKAFLP IYDFSYEMTT LLSPDERTAM 70 80 90 100 110 120 RQGVISQRTA WIHEQAKYYL NAGVPIEIKV VWHNRPFEAI IQEVISGGHD LVLKMAHQHD 130 140 150 160 170 180 RLEAVIFTPT DWHLLRKCPS PVWMVKDQPW PEGGKALVAV NLASEEPYHN ALNEKLVKET 190 200 210 220 230 240 IELAEQVNHT EVHLVGAYPV TPINIAIELP EFDPSVYNDA IRGQHLLAMK ALRQKFGINE 250 260 270 280 290 300 NMTHVEKGLP EEVIPDLAEH LQAGIVVLGT VGRTGISAAF LGNTAEQVID HLRCDLLVIK 310 PDQYQTPVEL DDEEDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Nucleotide sequence of the fnr gene and primary structure of the Enr protein of Escherichia coli." Shaw D.J., Guest J.R. Nucleic Acids Res. 10:6119-6130(1982) [PubMed: 6292868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
[5]	"Protein identification with N and C-terminal sequence tags in proteome projects." Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C., Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L., Hochstrasser D.F. J. Mol. Biol. 278:599-608(1998) [PubMed: 9600841] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-5. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B. Electrophoresis 20:2181-2195(1999) [PubMed: 10493123] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: B / BL21.
[7]	"The universal stress protein paralogues of Escherichia coli are co-ordinately regulated and co-operate in the defence against DNA damage." Gustavsson N., Diez A., Nystroem T. Mol. Microbiol. 43:107-117(2002) [PubMed: 11849540] [Abstract] Cited for: FUNCTION, GENE NAME, TRANSCRIPTIONAL REGULATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74415.1. AP009048 Genomic DNA. Translation: BAA14926.1. J01608 Genomic DNA. Translation: AAA87982.1.
PIR	QQECX. H64882.
RefSeq	NP_415849.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AAC0.
SMR	P0AAC0. Positions 4-308.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48118N.
2D gel databases
SWISS-2DPAGE	P0AAC0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000050; EBESCP00000000050; EBESCG00000000039. EBESCT00000015655; EBESCP00000014946; EBESCG00000014715.
GeneID	945904.
GenomeReviews	Gene locus JW1327 in contig AP009048_GR. Gene locus b1333 in contig U00096_GR.
KEGG	ecj:JW1327. eco:b1333.
PATRIC	32117942. VBIEscCol129921_1391.
Organism-specific databases
EchoBASE	EB1227.
EcoGene	EG11246. uspE.
Phylogenomic databases
eggNOG	COG0589.
GeneTree	EBGT00050000011173.
HOGENOM	HBG638855.
OMA	IDEDHTH.
PhylomeDB	P0AAC0.
ProtClustDB	PRK11175.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11246-MONOMER.
Gene expression databases
Genevestigator	P0AAC0.
Family and domain databases
InterPro	IPR014729. Rossmann-like_a/b/a_fold. IPR006016. UspA. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K14055.
Pfam	PF00582. Usp. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

USPE_ECOLI

Accession

Primary (citable) accession number: P0AAC0
Secondary accession number(s): P03807, P77421

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program