P0AAB7 (GALF_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UTP--glucose-1-phosphate uridylyltransferase
EC=2.7.7.9

Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
Short name=UDPGP
Uridine diphosphoglucose pyrophosphorylase

Gene names

Name:	galF
Synonyms:	wcaN
Ordered Locus Names:	Z3205, ECs2846

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Pathway	Carbohydrate metabolism; nucleotide-sugar metabolism. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Sequence similarities	Belongs to the UDPGP type 2 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC enzyme regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	UTP--glucose-1-phosphate uridylyltransferase		PRO_0000201350

Sequences

	Sequence		Length	Mass (Da)
	P0AAB7 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 6F85D6BB12C0C5A8	FASTA	297	32,829
	10 20 30 40 50 60 MTNLKAVIPV AGLGMHMLPA TKAIPKEMLP IVDKPMIQYI VDEIVAAGIK EILLVTHASK 70 80 90 100 110 120 NAVENHFDTS YELESLLEQR VKRQLLAEVQ SICPPGVTIM NVRQGEPLGL GHSILCARPA 130 140 150 160 170 180 IGDNPFVVVL PDVVIDDASA DPLRYNLAAM IARFNETGRS QVLAKRMPGD LSEYSVIQTK 190 200 210 220 230 240 EPLDREGKVS RIVEFIEKPD QPQTLDSDIM AVGRYVLSAD IWPELERTQP GAWGRIQLTD 250 260 270 280 290 AIAELAKKQS VDAMLMTGDS YDCGKKMGYM QAFVKYGLRN LKEGAKFRKG IEKLLSE

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG57101.1. BA000007 Genomic DNA. Translation: BAB36269.1.
PIR	A85830. F90984.
RefSeq	NP_288547.1. NC_002655.2. NP_310873.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0AAB7.
SMR	P0AAB7. Positions 1-293.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000027345; EBESCP00000026238; EBESCG00000026397. EBESCT00000059751; EBESCP00000057579; EBESCG00000058798.
GeneID	914113. 962100.
GenomeReviews	Gene locus Z3205 in contig AE005174_GR. Gene locus ECs2846 in contig BA000007_GR.
KEGG	ece:Z3205. ecs:ECs2846.
PATRIC	18355076. VBIEscCol44059_2739.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009639.
HOGENOM	HBG688195.
OMA	YVLPPRI.
ProtClustDB	PRK10122.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2846-MONOMER.
Family and domain databases
InterPro	IPR005774. GalF. IPR005835. NTP_transferase. [Graphical view]
KO	K00963.
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01105. GalF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALF_ECO57

Accession

Primary (citable) accession number: P0AAB7
Secondary accession number(s): P71245, P78083, P97192

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program