Reviewed, UniProtKB/Swiss-Prot P0AAB6 (GALF_ECOLI)

Names and origin

Protein names

Recommended name:
    UTP--glucose-1-phosphate uridylyltransferase
    EC=2.7.7.9
Alternative name(s):
    UDP-glucose pyrophosphorylase
      Short name=UDPGP
    Alpha-D-glucosyl-1-phosphate uridylyltransferase
    Uridine diphosphoglucose pyrophosphorylase

Gene names

Name:	galF
Synonyms:	wcaN
Ordered Locus Names:	b2042, JW2027

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Pathway	Carbohydrate metabolism; nucleotide-sugar metabolism. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Sequence similarities	Belongs to the UDPGP type 2 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC enzyme regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	UTP--glucose-1-phosphate uridylyltransferase		PRO_0000201349

Sequences

	Sequence		Length	Mass (Da)
	P0AAB6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 6F85D6BB12C0C5A8	FASTA	297	32,829
	10 20 30 40 50 60 MTNLKAVIPV AGLGMHMLPA TKAIPKEMLP IVDKPMIQYI VDEIVAAGIK EILLVTHASK 70 80 90 100 110 120 NAVENHFDTS YELESLLEQR VKRQLLAEVQ SICPPGVTIM NVRQGEPLGL GHSILCARPA 130 140 150 160 170 180 IGDNPFVVVL PDVVIDDASA DPLRYNLAAM IARFNETGRS QVLAKRMPGD LSEYSVIQTK 190 200 210 220 230 240 EPLDREGKVS RIVEFIEKPD QPQTLDSDIM AVGRYVLSAD IWPELERTQP GAWGRIQLTD 250 260 270 280 290 AIAELAKKQS VDAMLMTGDS YDCGKKMGYM QAFVKYGLRN LKEGAKFRKG IEKLLSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The GalF protein of Escherichia coli is not a UDP-glucose pyrophosphorylase but interacts with the GalU protein possibly to regulate cellular levels of UDP-glucose." Marolda C.L., Valvano M.A. Mol. Microbiol. 22:827-840(1996) [PubMed: 8971705] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U38473 Genomic DNA. Translation: AAC77853.1. U00096 Genomic DNA. Translation: AAC75103.1. AP009048 Genomic DNA. Translation: BAA15896.1.
PIR	A64970.
RefSeq	AP_002642.1. NP_416546.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	946560.
GenomeReviews	Gene locus JW2027 in contig AP009048_GR. Gene locus b2042 in contig U00096_GR.
KEGG	ecj:JW2027. eco:b2042.
Organism-specific databases
EchoBASE	EB3348.
EcoGene	EG13578. galF.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AAB6.
OMA	P0AAB6. MDEIMTN.
Enzyme and pathway databases
BioCyc	EcoCyc:G7093-MON. MetaCyc:G7093-MON.
Family and domain databases
InterPro	IPR005774. GalF. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01105. galF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALF_ECOLI

Accession

Primary (citable) accession number: P0AAB6
Secondary accession number(s): P71245, P78083, P97192

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)