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P0AAB2

- WZB_ECOLI

UniProt

P0AAB2 - WZB_ECOLI

Protein

Low molecular weight protein-tyrosine-phosphatase wzb

Gene

wzb

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Dephosphorylates wzc. Required for the extracellular polysaccharide colanic acid synthesis. Probably involved in the export of colanic acid from the cell to medium.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei9 – 91NucleophileBy similarity
    Active sitei14 – 141By similarity
    Active sitei115 – 1151Proton donorBy similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: EcoCyc

    GO - Biological processi

    1. colanic acid biosynthetic process Source: EcoCyc
    2. peptidyl-tyrosine dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Exopolysaccharide synthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:G7106-MONOMER.
    ECOL316407:JW2046-MONOMER.
    MetaCyc:G7106-MONOMER.
    UniPathwayiUPA00631.

    Protein family/group databases

    PptaseDBiP3D0409135.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Low molecular weight protein-tyrosine-phosphatase wzb (EC:3.1.3.48)
    Gene namesi
    Name:wzb
    Ordered Locus Names:b2061, JW2046
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13567. wzb.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 147147Low molecular weight protein-tyrosine-phosphatase wzbPRO_0000046572Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP0AAB2.

    Interactioni

    Protein-protein interaction databases

    IntActiP0AAB2. 17 interactions.
    MINTiMINT-8293227.
    STRINGi511145.b2061.

    Structurei

    Secondary structure

    1
    147
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 1411
    Helixi15 – 2612
    Beta strandi31 – 366
    Helixi47 – 559
    Helixi70 – 756
    Beta strandi76 – 827
    Helixi84 – 9310
    Helixi95 – 1006
    Beta strandi101 – 1033
    Helixi104 – 1074
    Turni108 – 1103
    Helixi121 – 14323

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FEKNMR-A1-147[»]
    ProteinModelPortaliP0AAB2.
    SMRiP0AAB2. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AAB2.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0394.
    HOGENOMiHOG000273091.
    KOiK01104.
    OMAiCELAPEM.
    OrthoDBiEOG63VC26.
    PhylomeDBiP0AAB2.

    Family and domain databases

    InterProiIPR023485. Ptyr_pPase_SF.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view]
    PANTHERiPTHR11717. PTHR11717. 1 hit.
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    PRINTSiPR00719. LMWPTPASE.
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AAB2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFNNILVVCV GNICRSPTAE RLLQRYHPEL KVESAGLGAL VGKGADPTAI    50
    SVAAEHQLSL EGHCARQISR RLCRNYDLIL TMEKRHIERL CEMAPEMRGK 100
    VMLFGHWDNE CEIPDPYRKS RETFAAVYTL LERSARQWAQ ALNAEQV 147
    Length:147
    Mass (Da):16,709
    Last modified:September 13, 2005 - v1
    Checksum:i6E36D7CE1038133B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38473 Genomic DNA. Translation: AAC77834.1.
    U00096 Genomic DNA. Translation: AAC75122.1.
    AP009048 Genomic DNA. Translation: BAE76575.1.
    PIRiD64972.
    RefSeqiNP_416565.1. NC_000913.3.
    YP_490303.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75122; AAC75122; b2061.
    BAE76575; BAE76575; BAE76575.
    GeneIDi12931963.
    946564.
    KEGGiecj:Y75_p2024.
    eco:b2061.
    PATRICi32119455. VBIEscCol129921_2138.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38473 Genomic DNA. Translation: AAC77834.1 .
    U00096 Genomic DNA. Translation: AAC75122.1 .
    AP009048 Genomic DNA. Translation: BAE76575.1 .
    PIRi D64972.
    RefSeqi NP_416565.1. NC_000913.3.
    YP_490303.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FEK NMR - A 1-147 [» ]
    ProteinModelPortali P0AAB2.
    SMRi P0AAB2. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0AAB2. 17 interactions.
    MINTi MINT-8293227.
    STRINGi 511145.b2061.

    Protein family/group databases

    PptaseDBi P3D0409135.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75122 ; AAC75122 ; b2061 .
    BAE76575 ; BAE76575 ; BAE76575 .
    GeneIDi 12931963.
    946564.
    KEGGi ecj:Y75_p2024.
    eco:b2061.
    PATRICi 32119455. VBIEscCol129921_2138.

    Organism-specific databases

    EchoBASEi EB3337.
    EcoGenei EG13567. wzb.

    Phylogenomic databases

    eggNOGi COG0394.
    HOGENOMi HOG000273091.
    KOi K01104.
    OMAi CELAPEM.
    OrthoDBi EOG63VC26.
    PhylomeDBi P0AAB2.

    Enzyme and pathway databases

    UniPathwayi UPA00631 .
    BioCyci EcoCyc:G7106-MONOMER.
    ECOL316407:JW2046-MONOMER.
    MetaCyc:G7106-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AAB2.
    PROi P0AAB2.

    Gene expression databases

    Genevestigatori P0AAB2.

    Family and domain databases

    InterProi IPR023485. Ptyr_pPase_SF.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view ]
    PANTHERi PTHR11717. PTHR11717. 1 hit.
    Pfami PF01451. LMWPc. 1 hit.
    [Graphical view ]
    PRINTSi PR00719. LMWPTPASE.
    SMARTi SM00226. LMWPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52788. SSF52788. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid."
      Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.
      J. Bacteriol. 178:4885-4893(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Cells of Escherichia coli contain a protein-tyrosine kinase, Wzc, and a phosphotyrosine-protein phosphatase, Wzb."
      Vincent C., Doublet P., Grangeasse C., Vaganay E., Cozzone A.J., Duclos B.
      J. Bacteriol. 181:3472-3477(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12 / JM109 / ATCC 53323.
    5. "Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in Gram-negative bacteria."
      Vincent C., Duclos B., Grangeasse C., Vaganay E., Riberty M., Cozzone A.J., Doublet P.
      J. Mol. Biol. 304:311-321(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12 / JM109 / ATCC 53323.

    Entry informationi

    Entry nameiWZB_ECOLI
    AccessioniPrimary (citable) accession number: P0AAB2
    Secondary accession number(s): P77153, Q2MAY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3