Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Low molecular weight protein-tyrosine-phosphatase Wzb

Gene

wzb

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates Wzc (PubMed:10348860). Required for the extracellular polysaccharide colanic acid synthesis, probably involved in the export of colanic acid from the cell to medium (PubMed:11090276). Involved in protection of cells against contact-dependent growth inhibition (CDI), probably due to the loss of a physical impediment to cell-cell contact.3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Pathwayi: exopolysaccharide biosynthesis

This protein is involved in the pathway exopolysaccharide biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway exopolysaccharide biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei9NucleophileBy similarity1
Active sitei14By similarity1
Active sitei115Proton donorBy similarity1

GO - Molecular functioni

  • protein tyrosine phosphatase activity Source: EcoCyc

GO - Biological processi

  • colanic acid biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Exopolysaccharide synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7106-MONOMER.
ECOL316407:JW2046-MONOMER.
MetaCyc:G7106-MONOMER.
UniPathwayiUPA00631.

Names & Taxonomyi

Protein namesi
Recommended name:
Low molecular weight protein-tyrosine-phosphatase Wzb (EC:3.1.3.481 Publication)
Gene namesi
Name:wzb
Ordered Locus Names:b2061, JW2046
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13567. wzb.

Pathology & Biotechi

Disruption phenotypei

No phosphorylation of Wzc (PubMed:11090276). Loss of mucoid colony phenotype, greatly increased susceptibility to contact-dependent growth inhibition (CDI) (PubMed:18761695).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000465721 – 147Low molecular weight protein-tyrosine-phosphatase WzbAdd BLAST147

Proteomic databases

PaxDbiP0AAB2.
PRIDEiP0AAB2.

Interactioni

Protein-protein interaction databases

BioGridi4260957. 423 interactors.
IntActiP0AAB2. 17 interactors.
MINTiMINT-8293227.
STRINGi511145.b2061.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 14Combined sources11
Helixi15 – 26Combined sources12
Beta strandi31 – 36Combined sources6
Helixi47 – 55Combined sources9
Helixi70 – 75Combined sources6
Beta strandi76 – 82Combined sources7
Helixi84 – 93Combined sources10
Helixi95 – 100Combined sources6
Beta strandi101 – 103Combined sources3
Helixi104 – 107Combined sources4
Turni108 – 110Combined sources3
Helixi121 – 143Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FEKNMR-A1-147[»]
ProteinModelPortaliP0AAB2.
SMRiP0AAB2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AAB2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105KJF. Bacteria.
COG0394. LUCA.
HOGENOMiHOG000273091.
InParanoidiP0AAB2.
KOiK01104.
OMAiCEIAPEM.
PhylomeDBiP0AAB2.

Family and domain databases

CDDicd00115. LMWPc. 1 hit.
InterProiIPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF20. PTHR11717:SF20. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AAB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNNILVVCV GNICRSPTAE RLLQRYHPEL KVESAGLGAL VGKGADPTAI
60 70 80 90 100
SVAAEHQLSL EGHCARQISR RLCRNYDLIL TMEKRHIERL CEMAPEMRGK
110 120 130 140
VMLFGHWDNE CEIPDPYRKS RETFAAVYTL LERSARQWAQ ALNAEQV
Length:147
Mass (Da):16,709
Last modified:September 13, 2005 - v1
Checksum:i6E36D7CE1038133B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77834.1.
U00096 Genomic DNA. Translation: AAC75122.1.
AP009048 Genomic DNA. Translation: BAE76575.1.
PIRiD64972.
RefSeqiNP_416565.1. NC_000913.3.
WP_000482901.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75122; AAC75122; b2061.
BAE76575; BAE76575; BAE76575.
GeneIDi946564.
KEGGiecj:JW2046.
eco:b2061.
PATRICi32119455. VBIEscCol129921_2138.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77834.1.
U00096 Genomic DNA. Translation: AAC75122.1.
AP009048 Genomic DNA. Translation: BAE76575.1.
PIRiD64972.
RefSeqiNP_416565.1. NC_000913.3.
WP_000482901.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FEKNMR-A1-147[»]
ProteinModelPortaliP0AAB2.
SMRiP0AAB2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260957. 423 interactors.
IntActiP0AAB2. 17 interactors.
MINTiMINT-8293227.
STRINGi511145.b2061.

Proteomic databases

PaxDbiP0AAB2.
PRIDEiP0AAB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75122; AAC75122; b2061.
BAE76575; BAE76575; BAE76575.
GeneIDi946564.
KEGGiecj:JW2046.
eco:b2061.
PATRICi32119455. VBIEscCol129921_2138.

Organism-specific databases

EchoBASEiEB3337.
EcoGeneiEG13567. wzb.

Phylogenomic databases

eggNOGiENOG4105KJF. Bacteria.
COG0394. LUCA.
HOGENOMiHOG000273091.
InParanoidiP0AAB2.
KOiK01104.
OMAiCEIAPEM.
PhylomeDBiP0AAB2.

Enzyme and pathway databases

UniPathwayiUPA00631.
BioCyciEcoCyc:G7106-MONOMER.
ECOL316407:JW2046-MONOMER.
MetaCyc:G7106-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AAB2.
PROiP0AAB2.

Family and domain databases

CDDicd00115. LMWPc. 1 hit.
InterProiIPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF20. PTHR11717:SF20. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiWZB_ECOLI
AccessioniPrimary (citable) accession number: P0AAB2
Secondary accession number(s): P77153, Q2MAY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.