P0AAB2 (WZB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Low molecular weight protein-tyrosine-phosphatase wzb
EC=3.1.3.48

Gene names

Name:	wzb
Ordered Locus Names:	b2061, JW2046

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dephosphorylates wzc. Required for the extracellular polysaccharide colanic acid synthesis. Probably involved in the export of colanic acid from the cell to medium.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Pathway	Glycan metabolism; exopolysaccharide biosynthesis.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

Ontologies

Keywords
Biological process	Exopolysaccharide synthesis
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	colanic acid biosynthetic process Inferred from mutant phenotype Ref.5. Source: EcoCyc
Molecular_function	protein tyrosine phosphatase activity Inferred from direct assay Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 147

147

Low molecular weight protein-tyrosine-phosphatase wzb

PRO_0000046572

Sites

Active site

9

1

Nucleophile By similarity

Active site

14

1

By similarity

Active site

115

1

Proton donor By similarity

Secondary structure

1

.

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147

Helix Strand Turn

Details...

Beta strand

4 – 14

11

Helix

15 – 26

12

Beta strand

31 – 36

6

Helix

47 – 55

9

Helix

70 – 75

6

Beta strand

76 – 82

7

Helix

84 – 93

10

Helix

95 – 100

6

Beta strand

101 – 103

3

Helix

104 – 107

4

Turn

108 – 110

3

Helix

121 – 143

23

Sequences

	Sequence		Length	Mass (Da)
	P0AAB2 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 6E36D7CE1038133B	FASTA	147	16,709
	10 20 30 40 50 60 MFNNILVVCV GNICRSPTAE RLLQRYHPEL KVESAGLGAL VGKGADPTAI SVAAEHQLSL 70 80 90 100 110 120 EGHCARQISR RLCRNYDLIL TMEKRHIERL CEMAPEMRGK VMLFGHWDNE CEIPDPYRKS 130 140 RETFAAVYTL LERSARQWAQ ALNAEQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid." Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R. J. Bacteriol. 178:4885-4893(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Cells of Escherichia coli contain a protein-tyrosine kinase, Wzc, and a phosphotyrosine-protein phosphatase, Wzb." Vincent C., Doublet P., Grangeasse C., Vaganay E., Cozzone A.J., Duclos B. J. Bacteriol. 181:3472-3477(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / JM109 / ATCC 53323.
[5]	"Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in Gram-negative bacteria." Vincent C., Duclos B., Grangeasse C., Vaganay E., Riberty M., Cozzone A.J., Doublet P. J. Mol. Biol. 304:311-321(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / JM109 / ATCC 53323.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U38473 Genomic DNA. Translation: AAC77834.1.
U00096 Genomic DNA. Translation: AAC75122.1.
AP009048 Genomic DNA. Translation: BAE76575.1.

PIR

D64972.

RefSeq

NP_416565.1. NC_000913.2.
YP_490303.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FEK	NMR	-	A	1-147	[»]

ProteinModelPortal

P0AAB2.

SMR

P0AAB2. Positions 1-147.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0AAB2. 16 interactions.

STRING

511145.b2061.

Protein family/group databases

PptaseDB

P3D0409135.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC75122; AAC75122; b2061.
BAE76575; BAE76575; BAE76575.

GeneID

12931963.
946564.

KEGG

ecj:Y75_p2024.
eco:b2061.

PATRIC

32119455. VBIEscCol129921_2138.

Organism-specific databases

EchoBASE

EB3337.

EcoGene

EG13567. wzb.

Phylogenomic databases

eggNOG

COG0394.

HOGENOM

HOG000273091.

KO

K01104.

OMA

QISRSLC.

ProtClustDB

PRK10126.

Enzyme and pathway databases

BioCyc

EcoCyc:G7106-MONOMER.
ECOL316407:JW2046-MONOMER.
MetaCyc:G7106-MONOMER.

UniPathway

UPA00631.

Gene expression databases

Genevestigator

P0AAB2.

Family and domain databases

InterPro

IPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]

PANTHER

PTHR11717. PTHR11717. 1 hit.

Pfam

PF01451. LMWPc. 1 hit.
[Graphical view]

PRINTS

PR00719. LMWPTPASE.

SMART

SM00226. LMWPc. 1 hit.
[Graphical view]

SUPFAM

SSF52788. Tyr_Pase_low_mol_wt. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0AAB2.

Entry information

Entry name

WZB_ECOLI

Accession

Primary (citable) accession number: P0AAB2
Secondary accession number(s): P77153, Q2MAY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program