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Protein

Low molecular weight protein-tyrosine-phosphatase Wzb

Gene

wzb

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dephosphorylates Wzc (PubMed:10348860). Required for the extracellular polysaccharide colanic acid synthesis, probably involved in the export of colanic acid from the cell to medium (PubMed:11090276). Involved in protection of cells against contact-dependent growth inhibition (CDI), probably due to the loss of a physical impediment to cell-cell contact.3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Pathwayi: exopolysaccharide biosynthesis

This protein is involved in the pathway exopolysaccharide biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway exopolysaccharide biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei9NucleophileBy similarity1
Active sitei14By similarity1
Active sitei115Proton donorBy similarity1

GO - Molecular functioni

  • protein tyrosine phosphatase activity Source: EcoCyc

GO - Biological processi

  • colanic acid biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processExopolysaccharide synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7106-MONOMER
MetaCyc:G7106-MONOMER
UniPathwayiUPA00631

Names & Taxonomyi

Protein namesi
Recommended name:
Low molecular weight protein-tyrosine-phosphatase Wzb (EC:3.1.3.481 Publication)
Gene namesi
Name:wzb
Ordered Locus Names:b2061, JW2046
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13567 wzb

Pathology & Biotechi

Disruption phenotypei

No phosphorylation of Wzc (PubMed:11090276). Loss of mucoid colony phenotype, greatly increased susceptibility to contact-dependent growth inhibition (CDI) (PubMed:18761695).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000465721 – 147Low molecular weight protein-tyrosine-phosphatase WzbAdd BLAST147

Proteomic databases

PaxDbiP0AAB2
PRIDEiP0AAB2

Interactioni

Protein-protein interaction databases

BioGridi4260957, 423 interactors
IntActiP0AAB2, 17 interactors
MINTiP0AAB2
STRINGi316385.ECDH10B_2211

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 14Combined sources11
Helixi15 – 26Combined sources12
Beta strandi31 – 36Combined sources6
Helixi47 – 55Combined sources9
Helixi70 – 75Combined sources6
Beta strandi76 – 82Combined sources7
Helixi84 – 93Combined sources10
Helixi95 – 100Combined sources6
Beta strandi101 – 103Combined sources3
Helixi104 – 107Combined sources4
Turni108 – 110Combined sources3
Helixi121 – 143Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FEKNMR-A1-147[»]
ProteinModelPortaliP0AAB2
SMRiP0AAB2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AAB2

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105KJF Bacteria
COG0394 LUCA
HOGENOMiHOG000273091
InParanoidiP0AAB2
KOiK01104
OMAiLQGEWHV
PhylomeDBiP0AAB2

Family and domain databases

InterProiView protein in InterPro
IPR023485 Ptyr_pPase
IPR036196 Ptyr_pPase_sf
IPR017867 Tyr_phospatase_low_mol_wt
PfamiView protein in Pfam
PF01451 LMWPc, 1 hit
PRINTSiPR00719 LMWPTPASE
SMARTiView protein in SMART
SM00226 LMWPc, 1 hit
SUPFAMiSSF52788 SSF52788, 1 hit

Sequencei

Sequence statusi: Complete.

P0AAB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNNILVVCV GNICRSPTAE RLLQRYHPEL KVESAGLGAL VGKGADPTAI
60 70 80 90 100
SVAAEHQLSL EGHCARQISR RLCRNYDLIL TMEKRHIERL CEMAPEMRGK
110 120 130 140
VMLFGHWDNE CEIPDPYRKS RETFAAVYTL LERSARQWAQ ALNAEQV
Length:147
Mass (Da):16,709
Last modified:September 13, 2005 - v1
Checksum:i6E36D7CE1038133B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA Translation: AAC77834.1
U00096 Genomic DNA Translation: AAC75122.1
AP009048 Genomic DNA Translation: BAE76575.1
PIRiD64972
RefSeqiNP_416565.1, NC_000913.3
WP_000482901.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75122; AAC75122; b2061
BAE76575; BAE76575; BAE76575
GeneIDi946564
KEGGiecj:JW2046
eco:b2061
PATRICifig|511145.12.peg.2138

Similar proteinsi

Entry informationi

Entry nameiWZB_ECOLI
AccessioniPrimary (citable) accession number: P0AAB2
Secondary accession number(s): P77153, Q2MAY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health