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Protein

Lipid III flippase

Gene

wzxE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the transbilayer movement of Und-PP-GlcNAc-ManNAcA-Fuc4NAc (lipid III) from the inner to the outer leaflet of the cytoplasmic membrane during the assembly of enterobacterial common antigen (ECA). Required for the assembly of the phosphoglyceride-linked form of ECA (ECA(PG)) and the water-soluble cyclic form of ECA (ECA(CYC)). Could also mediate the translocation of Und-PP-GlcNAc.2 Publications

Pathwayi: enterobacterial common antigen biosynthesis

This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation2 Publications
View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11486-MONOMER.
ECOL316407:JW3766-MONOMER.
MetaCyc:EG11486-MONOMER.
UniPathwayiUPA00566.

Protein family/group databases

TCDBi2.A.66.2.3. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid III flippaseUniRule annotationCurated
Gene namesi
Name:wzxE1 PublicationUniRule annotation
Synonyms:wzx, yifJ
Ordered Locus Names:b3792, JW3766
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11486. wzxE.

Subcellular locationi

  • Cell inner membrane UniRule annotationCurated; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17CytoplasmicCuratedAdd BLAST17
Transmembranei18 – 38HelicalUniRule annotationAdd BLAST21
Topological domaini39 – 45PeriplasmicCurated7
Transmembranei46 – 66HelicalUniRule annotationAdd BLAST21
Topological domaini67 – 84CytoplasmicCuratedAdd BLAST18
Transmembranei85 – 105HelicalUniRule annotationAdd BLAST21
Topological domaini106 – 121PeriplasmicCuratedAdd BLAST16
Transmembranei122 – 142HelicalUniRule annotationAdd BLAST21
Topological domaini143 – 144CytoplasmicCurated2
Transmembranei145 – 165HelicalUniRule annotationAdd BLAST21
Topological domaini166 – 174PeriplasmicCurated9
Transmembranei175 – 195HelicalUniRule annotationAdd BLAST21
Topological domaini196 – 216CytoplasmicCuratedAdd BLAST21
Transmembranei217 – 237HelicalUniRule annotationAdd BLAST21
Topological domaini238 – 259PeriplasmicCuratedAdd BLAST22
Transmembranei260 – 280HelicalUniRule annotationAdd BLAST21
Topological domaini281 – 302CytoplasmicCuratedAdd BLAST22
Transmembranei303 – 323HelicalUniRule annotationAdd BLAST21
Topological domaini324 – 334PeriplasmicCuratedAdd BLAST11
Transmembranei335 – 355HelicalUniRule annotationAdd BLAST21
Topological domaini356 – 370CytoplasmicCuratedAdd BLAST15
Transmembranei371 – 391HelicalUniRule annotationAdd BLAST21
Transmembranei392 – 412HelicalUniRule annotationAdd BLAST21
Topological domaini413 – 416Cytoplasmic1 Publication4

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB-HAMAP
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Null mutation results in the accumulation of lipid III and cell death.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000659891 – 416Lipid III flippaseAdd BLAST416

Proteomic databases

PaxDbiP0AAA7.
PRIDEiP0AAA7.

Interactioni

Subunit structurei

Probably part of a complex composed of WzxE, WzyE and WzzE.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4262107. 228 interactors.
STRINGi511145.b3792.

Structurei

3D structure databases

ProteinModelPortaliP0AAA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide transport (PST) (TC 2.A.66.2) family. [View classification]UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EGZ. Bacteria.
COG2244. LUCA.
HOGENOMiHOG000126459.
InParanoidiP0AAA7.
KOiK16693.
OMAiYICFRIG.

Family and domain databases

HAMAPiMF_02024. WzxE. 1 hit.
InterProiIPR002797. Polysacc_synth.
IPR032896. WzxE.
[Graphical view]
PfamiPF01943. Polysacc_synt. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLAKASLWT AASTLVKIGA GLLVGKLLAV SFGPAGLGLA ANFRQLITVL
60 70 80 90 100
GVLAGAGIFN GVTKYVAQYH DNPQQLRRVV GTSSAMVLGF STLMALVFVL
110 120 130 140 150
AAAPISQGLF GNTDYQGLVR LVALVQMGIA WGNLLLALMK GFRDAAGNAL
160 170 180 190 200
SLIVGSLIGV LAYYVSYRLG GYEGALLGLA LIPALVVIPA AIMLIKRGVI
210 220 230 240 250
PLSYLKPSWD NGLAGQLSKF TLMALITSVT LPVAYIMMRK LLAAQYSWDE
260 270 280 290 300
VGIWQGVSSI SDAYLQFITA SFSVYLLPTL SRLTEKRDIT REVVKSLKFV
310 320 330 340 350
LPAVAAASFT VWLLRDFAIW LLLSNKFTAM RDLFAWQLVG DVLKVGAYVF
360 370 380 390 400
GYLVIAKASL RFYILAEVSQ FTLLMVFAHW LIPAHGALGA AQAYMATYIV
410
YFSLCCGVFL LWRRRA
Length:416
Mass (Da):44,960
Last modified:September 13, 2005 - v1
Checksum:i001690A4E86CADF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67592.1.
U00096 Genomic DNA. Translation: AAC76797.1.
AP009048 Genomic DNA. Translation: BAE77506.1.
PIRiC65183.
RefSeqiNP_418239.1. NC_000913.3.
WP_000050265.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76797; AAC76797; b3792.
BAE77506; BAE77506; BAE77506.
GeneIDi948294.
KEGGiecj:JW3766.
eco:b3792.
PATRICi32123077. VBIEscCol129921_3908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67592.1.
U00096 Genomic DNA. Translation: AAC76797.1.
AP009048 Genomic DNA. Translation: BAE77506.1.
PIRiC65183.
RefSeqiNP_418239.1. NC_000913.3.
WP_000050265.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAA7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262107. 228 interactors.
STRINGi511145.b3792.

Protein family/group databases

TCDBi2.A.66.2.3. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Proteomic databases

PaxDbiP0AAA7.
PRIDEiP0AAA7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76797; AAC76797; b3792.
BAE77506; BAE77506; BAE77506.
GeneIDi948294.
KEGGiecj:JW3766.
eco:b3792.
PATRICi32123077. VBIEscCol129921_3908.

Organism-specific databases

EchoBASEiEB1449.
EcoGeneiEG11486. wzxE.

Phylogenomic databases

eggNOGiENOG4105EGZ. Bacteria.
COG2244. LUCA.
HOGENOMiHOG000126459.
InParanoidiP0AAA7.
KOiK16693.
OMAiYICFRIG.

Enzyme and pathway databases

UniPathwayiUPA00566.
BioCyciEcoCyc:EG11486-MONOMER.
ECOL316407:JW3766-MONOMER.
MetaCyc:EG11486-MONOMER.

Miscellaneous databases

PROiP0AAA7.

Family and domain databases

HAMAPiMF_02024. WzxE. 1 hit.
InterProiIPR002797. Polysacc_synth.
IPR032896. WzxE.
[Graphical view]
PfamiPF01943. Polysacc_synt. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWZXE_ECOLI
AccessioniPrimary (citable) accession number: P0AAA7
Secondary accession number(s): P27834, Q2M8A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.