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Protein

Sensor histidine kinase YpdA

Gene

ypdA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system YpdA/YpdB, which is part of a nutrient-sensing regulatory network composed of YpdA/YpdB, the high-affinity pyruvate signaling system BtsS/BtsR and their respective target proteins, YhjX and BtsT. YpdA activates YpdB by phosphorylation in response to high concentrations of extracellular pyruvate. Activation of the YpdA/YpdB signaling cascade also promotes BtsS/BtsR-mediated btsT expression.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.Curated

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • cellular response to nutrient Source: EcoCyc
  • cell wall organization Source: InterPro
  • phosphorelay signal transduction system Source: EcoCyc
  • signal transduction by protein phosphorylation Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7243-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase YpdACurated (EC:2.7.13.3)
Gene namesi
Name:ypdA
Ordered Locus Names:b2380, JW5388
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14148 ypdA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3Cytoplasmic1 Publication3
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Topological domaini25 – 45Periplasmic1 PublicationAdd BLAST21
Transmembranei46 – 66HelicalSequence analysisAdd BLAST21
Topological domaini67 – 74Cytoplasmic1 Publication8
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Topological domaini96 – 107Periplasmic1 PublicationAdd BLAST12
Transmembranei108 – 128HelicalSequence analysisAdd BLAST21
Topological domaini129 – 139Cytoplasmic1 PublicationAdd BLAST11
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 172Periplasmic1 PublicationAdd BLAST12
Transmembranei173 – 193HelicalSequence analysisAdd BLAST21
Topological domaini194 – 565Cytoplasmic1 PublicationAdd BLAST372

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi371H → Q: Lack of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002013381 – 565Sensor histidine kinase YpdAAdd BLAST565

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei371Phosphohistidine; by autocatalysisBy similarity1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AA93
PRIDEiP0AA93

Interactioni

Subunit structurei

Interacts with BtsT and YhjX.1 Publication

Protein-protein interaction databases

BioGridi4260545, 186 interactors
STRINGi316385.ECDH10B_2545

Structurei

3D structure databases

ProteinModelPortaliP0AA93
SMRiP0AA93
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini223 – 342GAFAdd BLAST120
Domaini343 – 554Histidine kinaseAdd BLAST212

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108HRK Bacteria
COG3275 LUCA
HOGENOMiHOG000242218
InParanoidiP0AA93
KOiK02478
OMAiWEKGEVT
PhylomeDBiP0AA93

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di3.30.450.40, 1 hit
3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR029016 GAF-like_dom_sf
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR010559 Sig_transdc_His_kin_internal
IPR011620 Sig_transdc_His_kinase_LytS_TM
PfamiView protein in Pfam
PF07694 5TM-5TMR_LYT, 1 hit
PF02518 HATPase_c, 1 hit
PF06580 His_kinase, 1 hit
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF55874 SSF55874, 1 hit

Sequencei

Sequence statusi: Complete.

P0AA93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHEIFNMLLA VFDRAALMLI CLFFLIRIRL FRELLHKSAH SPKELLAVTA
60 70 80 90 100
IFSLFALFST WSGVPVEGSL VNVRIIAVMS GGILFGPWVG IITGVIAGIH
110 120 130 140 150
RYLIDIGGVT AIPCFITSIL AGCISGWINL KIPKAQRWRV GILGGMLCET
160 170 180 190 200
LTMILVIVWA PTTALGIDIV SKIGIPMILG SVCIGFIVLL VQSVEGEKEA
210 220 230 240 250
SAARQAKLAL DIANKTLPLF RHVNSESLRK VCEIIRDDIH ADAVAITNTD
260 270 280 290 300
HVLAYVGVGE HNYQNGDDFI SPTTRQAMNY GKIIIKNNDE AHRTPEIHSM
310 320 330 340 350
LVIPLWEKGV VTGTLKIYYC HAHQITSSLQ EMAVGLSQII STQLEVSRAE
360 370 380 390 400
QLREMANKAE LRALQSKINP HFLFNALNAI SSSIRLNPDT ARQLIFNLSR
410 420 430 440 450
YLRYNIELKD DEQIDIKKEL YQIKDYIAIE QARFGDKLTV IYDIDEEVNC
460 470 480 490 500
CIPSLLIQPL VENAIVHGIQ PCKGKGVVTI SVAECGNRVR IAVRDTGHGI
510 520 530 540 550
DPKVIERVEA NEMPGNKIGL LNVHHRVKLL YGEGLHIRRL EPGTEIAFYI
560
PNQRTPVASQ ATLLL
Length:565
Mass (Da):62,657
Last modified:September 13, 2005 - v1
Checksum:i1CD1B604BA5E48B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75439.1
AP009048 Genomic DNA Translation: BAA16250.2
PIRiA65012
RefSeqiNP_416881.1, NC_000913.3
WP_000544359.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75439; AAC75439; b2380
BAA16250; BAA16250; BAA16250
GeneIDi946723
KEGGiecj:JW5388
eco:b2380
PATRICifig|1411691.4.peg.4348

Similar proteinsi

Entry informationi

Entry nameiYPDA_ECOLI
AccessioniPrimary (citable) accession number: P0AA93
Secondary accession number(s): P76523, P76950
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health