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Protein

Thiol:disulfide interchange protein DsbE

Gene

dsbE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme lyase. DsbE is maintained in a reduced state by DsbD.

GO - Molecular functioni

  • disulfide oxidoreductase activity Source: EcoCyc

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • cytochrome complex assembly Source: EcoCyc
  • oxidation-reduction process Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Cytochrome c-type biogenesis

Enzyme and pathway databases

BioCyciEcoCyc:EG12053-MONOMER.
ECOL316407:JW2183-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiol:disulfide interchange protein DsbE
Alternative name(s):
Cytochrome c biogenesis protein CcmG
Gene namesi
Name:dsbE
Synonyms:ccmG, yejQ
Ordered Locus Names:b2195, JW2183
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12053. dsbE.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 25HelicalSequence analysisAdd BLAST21
Topological domaini26 – 185PeriplasmicSequence analysisAdd BLAST160

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi80C → S: Drastic decrease in activity. 1 Publication1
Mutagenesisi83C → S: Drastic decrease in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002012931 – 185Thiol:disulfide interchange protein DsbEAdd BLAST185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi80 ↔ 83Redox-activeCurated

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0AA86.
PRIDEiP0AA86.

Interactioni

Protein-protein interaction databases

BioGridi4263405. 11 interactors.
DIPiDIP-48446N.
STRINGi511145.b2195.

Structurei

Secondary structure

1185
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 38Combined sources3
Turni39 – 42Combined sources4
Beta strandi49 – 55Combined sources7
Beta strandi59 – 61Combined sources3
Helixi62 – 65Combined sources4
Beta strandi67 – 69Combined sources3
Beta strandi71 – 76Combined sources6
Helixi81 – 95Combined sources15
Beta strandi100 – 106Combined sources7
Helixi109 – 119Combined sources11
Beta strandi124 – 129Combined sources6
Helixi133 – 138Combined sources6
Beta strandi142 – 149Combined sources8
Beta strandi153 – 161Combined sources9
Helixi165 – 170Combined sources6
Helixi173 – 183Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z5YX-ray1.94E43-185[»]
2B1KX-ray1.90A19-185[»]
2B1LX-ray1.90A/B58-185[»]
2G0FX-ray2.20A19-185[»]
3K8NX-ray2.30A1-185[»]
ProteinModelPortaliP0AA86.
SMRiP0AA86.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AA86.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 177ThioredoxinPROSITE-ProRule annotationAdd BLAST139

Sequence similaritiesi

Belongs to the thioredoxin family. DsbE subfamily.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107QX9. Bacteria.
COG0526. LUCA.
HOGENOMiHOG000097218.
InParanoidiP0AA86.
KOiK02199.
OMAiKWLAEFH.
PhylomeDBiP0AA86.

Family and domain databases

CDDicd03010. TlpA_like_DsbE. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR004799. Periplasmic_diS_OxRdtase_DsbE.
IPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF08534. Redoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00385. dsbE. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AA86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKVLLIPL IIFLAIAAAL LWQLARNAEG DDPTNLESAL IGKPVPKFRL
60 70 80 90 100
ESLDNPGQFY QADVLTQGKP VLLNVWATWC PTCRAEHQYL NQLSAQGIRV
110 120 130 140 150
VGMNYKDDRQ KAISWLKELG NPYALSLFDG DGMLGLDLGV YGAPETFLID
160 170 180
GNGIIRYRHA GDLNPRVWEE EIKPLWEKYS KEAAQ
Length:185
Mass (Da):20,809
Last modified:September 13, 2005 - v1
Checksum:iA599F7B77806A6B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA. Translation: AAA16387.1.
U00096 Genomic DNA. Translation: AAC75255.1.
AP009048 Genomic DNA. Translation: BAE76658.1.
PIRiA64989.
RefSeqiNP_416699.1. NC_000913.3.
WP_000824439.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75255; AAC75255; b2195.
BAE76658; BAE76658; BAE76658.
GeneIDi949073.
KEGGiecj:JW2183.
eco:b2195.
PATRICi32119749. VBIEscCol129921_2284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA. Translation: AAA16387.1.
U00096 Genomic DNA. Translation: AAC75255.1.
AP009048 Genomic DNA. Translation: BAE76658.1.
PIRiA64989.
RefSeqiNP_416699.1. NC_000913.3.
WP_000824439.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z5YX-ray1.94E43-185[»]
2B1KX-ray1.90A19-185[»]
2B1LX-ray1.90A/B58-185[»]
2G0FX-ray2.20A19-185[»]
3K8NX-ray2.30A1-185[»]
ProteinModelPortaliP0AA86.
SMRiP0AA86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263405. 11 interactors.
DIPiDIP-48446N.
STRINGi511145.b2195.

Proteomic databases

PaxDbiP0AA86.
PRIDEiP0AA86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75255; AAC75255; b2195.
BAE76658; BAE76658; BAE76658.
GeneIDi949073.
KEGGiecj:JW2183.
eco:b2195.
PATRICi32119749. VBIEscCol129921_2284.

Organism-specific databases

EchoBASEiEB1984.
EcoGeneiEG12053. dsbE.

Phylogenomic databases

eggNOGiENOG4107QX9. Bacteria.
COG0526. LUCA.
HOGENOMiHOG000097218.
InParanoidiP0AA86.
KOiK02199.
OMAiKWLAEFH.
PhylomeDBiP0AA86.

Enzyme and pathway databases

BioCyciEcoCyc:EG12053-MONOMER.
ECOL316407:JW2183-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AA86.
PROiP0AA86.

Family and domain databases

CDDicd03010. TlpA_like_DsbE. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR004799. Periplasmic_diS_OxRdtase_DsbE.
IPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF08534. Redoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00385. dsbE. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDSBE_ECOLI
AccessioniPrimary (citable) accession number: P0AA86
Secondary accession number(s): P33926, Q2MAP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.