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Protein

Cardiolipin synthase B

Gene

clsB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. Can also catalyze phosphatidyl group transfer to water to form phosphatidate.UniRule annotation2 Publications

Miscellaneous

All three cardiolipin synthases (ClsA, ClsB and ClsC) contribute to CL synthesis in stationary phase. Only ClsA contributes to synthesis during logarithmic growth phase.1 Publication

Catalytic activityi

2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol.UniRule annotation1 Publication

Enzyme regulationi

Activated by phosphate. Inhibited by cardiolipin and phosphatidate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei113UniRule annotation1
Active sitei115UniRule annotation1
Active sitei120UniRule annotation1
Active sitei290UniRule annotation1
Active sitei292UniRule annotation1
Active sitei297UniRule annotation1

GO - Molecular functioni

  • cardiolipin synthase activity Source: EcoCyc
  • phospholipase D activity Source: EcoliWiki

GO - Biological processi

  • cardiolipin biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionTransferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:G6406-MONOMER
MetaCyc:G6406-MONOMER
BRENDAi2.7.8.B10 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Cardiolipin synthase BUniRule annotation (EC:2.7.8.-UniRule annotation1 Publication)
Short name:
CL synthaseUniRule annotation
Gene namesi
Name:clsB1 PublicationUniRule annotation
Synonyms:ybhO
Ordered Locus Names:b0789, JW0772
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13671 clsB

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  • membrane Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Triple deletion of clsA, clsB and clsC results in a complete lack of cardiolipin, regardless of growth phase or growth conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002012831 – 413Cardiolipin synthase BAdd BLAST413

Proteomic databases

PaxDbiP0AA84
PRIDEiP0AA84

Interactioni

Protein-protein interaction databases

BioGridi4259959, 389 interactors
IntActiP0AA84, 5 interactors
STRINGi316385.ECDH10B_0857

Structurei

3D structure databases

ProteinModelPortaliP0AA84
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 135PLD phosphodiesterase 1UniRule annotationAdd BLAST28
Domaini285 – 312PLD phosphodiesterase 2UniRule annotationAdd BLAST28

Sequence similaritiesi

Belongs to the phospholipase D family. Cardiolipin synthase subfamily. ClsB sub-subfamily.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DZQ Bacteria
COG1502 LUCA
HOGENOMiHOG000264393
InParanoidiP0AA84
KOiK06131
OMAiVLFLQMW
PhylomeDBiP0AA84

Family and domain databases

HAMAPiMF_01917 Cardiolipin_synth_ClsB, 1 hit
InterProiView protein in InterPro
IPR030872 Cardiolipin_synth_ClsB
IPR025202 PLD-like_dom
IPR001736 PLipase_D/transphosphatidylase
PfamiView protein in Pfam
PF13091 PLDc_2, 2 hits
SMARTiView protein in SMART
SM00155 PLDc, 2 hits
PROSITEiView protein in PROSITE
PS50035 PLD, 2 hits

Sequencei

Sequence statusi: Complete.

P0AA84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCSWREGNK IQLLENGEQY YPAVFKAIGE AQERIILETF IWFEDDVGKQ
60 70 80 90 100
LHAALLAAAQ RGVKAEVLLD GYGSPDLSDE FVNELTAAGV VFRYYDPRPR
110 120 130 140 150
LFGMRTNVFR RMHRKIVVID ARIAFIGGLN YSAEHMSSYG PEAKQDYAVR
160 170 180 190 200
LEGPIVEDIL QFELENLPGQ SAARRWWRRH HKAEENRQPG EAQVLLVWRD
210 220 230 240 250
NEEHRDDIER HYLKMLTQAR REVIIANAYF FPGYRFLHAL RKAARRGVRI
260 270 280 290 300
KLIIQGEPDM PIVRVGARLL YNYLVKGGVQ VFEYRRRPLH GKVALMDDHW
310 320 330 340 350
ATVGSSNLDP LSLSLNLEAN VIIHDRHFNQ TLRDNLNGII AADCQQVDET
360 370 380 390 400
MLPKRTWWNL TKSVLAFHFL RHFPALVGWL PAHTPRLAQV DPPAQPTMET
410
QDRVETENTG VKP
Length:413
Mass (Da):47,634
Last modified:September 13, 2005 - v1
Checksum:i74998B2A1AD24A11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73876.1
AP009048 Genomic DNA Translation: BAA35448.1
PIRiE64815
RefSeqiNP_415310.1, NC_000913.3
WP_000650337.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73876; AAC73876; b0789
BAA35448; BAA35448; BAA35448
GeneIDi945409
KEGGiecj:JW0772
eco:b0789
PATRICifig|1411691.4.peg.1489

Similar proteinsi

Entry informationi

Entry nameiCLSB_ECOLI
AccessioniPrimary (citable) accession number: P0AA84
Secondary accession number(s): P75771
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health