P0AA80 (GARP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable galactarate transporter

Alternative name(s):
D-galactarate permease

Gene names

Name:	garP
Synonyms:	yhaU
Ordered Locus Names:	b3127, JW3096

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Uptake of D-galactarate. Ref.3
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the major facilitator superfamily. Phthalate permease family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Probable galactarate transporter		PRO_0000121382
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Potential
	Topological domain	33 – 56	24	Periplasmic Potential
	Transmembrane	57 – 77	21	Helical; Potential
	Topological domain	78 – 89	12	Cytoplasmic Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Transmembrane	111 – 131	21	Helical; Potential
	Topological domain	132 – 157	26	Cytoplasmic Potential
	Transmembrane	158 – 178	21	Helical; Potential
	Transmembrane	179 – 199	21	Helical; Potential
	Topological domain	200 – 252	53	Cytoplasmic Potential
	Transmembrane	253 – 273	21	Helical; Potential
	Topological domain	274 – 288	15	Periplasmic Potential
	Transmembrane	289 – 309	21	Helical; Potential
	Topological domain	310 – 319	10	Cytoplasmic Potential
	Transmembrane	320 – 340	21	Helical; Potential
	Topological domain	341 – 350	10	Periplasmic Potential
	Transmembrane	351 – 371	21	Helical; Potential
	Topological domain	372 – 385	14	Cytoplasmic Potential
	Transmembrane	386 – 406	21	Helical; Potential
	Topological domain	407 – 413	7	Periplasmic Potential
	Transmembrane	414 – 434	21	Helical; Potential
	Topological domain	435 – 444	10	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AA80 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 48FA27023B0C9D30	FASTA	444	49,009
	10 20 30 40 50 60 MILDTVDEKK KGVHTRYLIL LIIFIVTAVN YADRATLSIA GTEVAKELQL SAVSMGYIFS 70 80 90 100 110 120 AFGWAYLLMQ IPGGWLLDKF GSKKVYTYSL FFWSLFTFLQ GFVDMFPLAW AGISMFFMRF 130 140 150 160 170 180 MLGFSEAPSF PANARIVAAW FPTKERGTAS AIFNSAQYFS LALFSPLLGW LTFAWGWEHV 190 200 210 220 230 240 FTVMGVIGFV LTALWIKLIH NPTDHPRMSA EELKFISENG AVVDMDHKKP GSAAASGPKL 250 260 270 280 290 300 HYIKQLLSNR MMLGVFFGQY FINTITWFFL TWFPIYLVQE KGMSILKVGL VASIPALCGF 310 320 330 340 350 360 AGGVLGGVFS DYLIKRGLSL TLARKLPIVL GMLLASTIIL CNYTNNTTLV VMLMALAFFG 370 380 390 400 410 420 KGFGALGWPV ISDTAPKEIV GLCGGVFNVF GNVASIVTPL VIGYLVSELH SFNAALVFVG 430 440 CSALMAMVCY LFVVGDIKRM ELQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli." Hubbard B.K., Koch M., Palmer D.R., Babbitt P.C., Gerlt J.A. Biochemistry 37:14369-14375(1998) [PubMed: 9772162] [Abstract] Cited for: POSSIBLE FUNCTION.
[4]	"A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli." Monterrubio R., Baldoma L., Obradors N., Aguilar J., Badia J. J. Bacteriol. 182:2672-2674(2000) [PubMed: 10762278] [Abstract] Cited for: GENE NAME.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA57930.1. U00096 Genomic DNA. Translation: AAC76161.1. AP009048 Genomic DNA. Translation: BAE77174.1.
PIR	C65102.
RefSeq	NP_417596.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AA80.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003327; EBESCP00000003327; EBESCG00000002727. EBESCT00000016082; EBESCP00000015373; EBESCG00000015142.
GeneID	947642.
GenomeReviews	Gene locus JW3096 in contig AP009048_GR. Gene locus b3127 in contig U00096_GR.
KEGG	ecj:JW3096. eco:b3127.
PATRIC	32121668. VBIEscCol129921_3220.
Organism-specific databases
EchoBASE	EB2613.
EcoGene	EG12760. garP.
Phylogenomic databases
eggNOG	COG0477.
GeneTree	EBGT00050000008818.
HOGENOM	HBG338043.
OMA	FPISKVG.
PhylomeDB	P0AA80.
ProtClustDB	CLSK880597.
Enzyme and pathway databases
BioCyc	EcoCyc:YHAU-MONOMER.
Gene expression databases
Genevestigator	P0AA80.
Family and domain databases
InterPro	IPR004744. Dgal_transporter. IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
KO	K12299.
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00893. 2A0114. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GARP_ECOLI

Accession

Primary (citable) accession number: P0AA80
Secondary accession number(s): P42613, Q2M982

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program