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Protein

Threonine/homoserine exporter RhtA

Gene

rhtA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the efflux of threonine and homoserine. Can also export other amino acids such as proline, serine, histidine and cysteine.1 Publication

GO - Molecular functioni

  • threonine efflux transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • threonine transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG12134-MONOMER.
ECOL316407:JW0798-MONOMER.
MetaCyc:EG12134-MONOMER.
RETL1328306-WGS:GSTH-257-MONOMER.

Protein family/group databases

TCDBi2.A.7.3.6. the drug/metabolite transporter (dmt) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine/homoserine exporter RhtA
Gene namesi
Name:rhtA
Synonyms:ybiF
Ordered Locus Names:b0813, JW0798
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12134. rhtA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Topological domaini31 – 388PeriplasmicSequence analysis
Transmembranei39 – 5921HelicalSequence analysisAdd
BLAST
Topological domaini60 – 7112CytoplasmicSequence analysisAdd
BLAST
Transmembranei72 – 9221HelicalSequence analysisAdd
BLAST
Topological domaini93 – 931PeriplasmicSequence analysis
Transmembranei94 – 11421HelicalSequence analysisAdd
BLAST
Topological domaini115 – 1184CytoplasmicSequence analysis
Transmembranei119 – 13921HelicalSequence analysisAdd
BLAST
Topological domaini140 – 1467PeriplasmicSequence analysis
Transmembranei147 – 16721HelicalSequence analysisAdd
BLAST
Topological domaini168 – 1758CytoplasmicSequence analysis
Transmembranei176 – 19621HelicalSequence analysisAdd
BLAST
Topological domaini197 – 2004PeriplasmicSequence analysis
Transmembranei201 – 22121HelicalSequence analysisAdd
BLAST
Topological domaini222 – 23716CytoplasmicSequence analysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Topological domaini259 – 2624PeriplasmicSequence analysis
Transmembranei263 – 28321HelicalSequence analysisAdd
BLAST
Topological domaini284 – 29512CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Threonine/homoserine exporter RhtAPRO_0000108162Add
BLAST

Proteomic databases

PaxDbiP0AA67.

Interactioni

Protein-protein interaction databases

BioGridi4259985. 13 interactions.
STRINGi511145.b0813.

Structurei

3D structure databases

ProteinModelPortaliP0AA67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 135106EamA 1Add
BLAST
Domaini159 – 278120EamA 2Add
BLAST

Sequence similaritiesi

Contains 2 EamA domains.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107AQZ. Bacteria.
COG5006. LUCA.
HOGENOMiHOG000283695.
InParanoidiP0AA67.
KOiK11939.
OMAiMALGGMN.
OrthoDBiEOG67T5NC.
PhylomeDBiP0AA67.

Family and domain databases

InterProiIPR000620. EamA_dom.
[Graphical view]
PfamiPF00892. EamA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AA67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGSLRKMPV WLPIVILLVA MASIQGGASL AKSLFPLVGA PGVTALRLAL
60 70 80 90 100
GTLILIAFFK PWRLRFAKEQ RLPLLFYGVS LGGMNYLFYL SIQTVPLGIA
110 120 130 140 150
VALEFTGPLA VALFSSRRPV DFVWVVLAVL GLWFLLPLGQ DVSHVDLTGC
160 170 180 190 200
ALALGAGACW AIYILSGQRA GAEHGPATVA IGSLIAALIF VPIGALQAGE
210 220 230 240 250
ALWHWSVIPL GLAVAILSTA LPYSLEMIAL TRLPTRTFGT LMSMEPALAA
260 270 280 290
VSGMIFLGET LTPIQLLALG AIIAASMGST LTVRKESKIK ELDIN
Length:295
Mass (Da):31,168
Last modified:September 13, 2005 - v1
Checksum:iEC830029E71BAD5F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti268 – 2681A → R in AAA21854 (PubMed:8021175).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04242 Genomic DNA. Translation: AAA21854.1.
U00096 Genomic DNA. Translation: AAC73900.1.
AP009048 Genomic DNA. Translation: BAA35485.1.
PIRiE64818.
RefSeqiNP_415334.1. NC_000913.3.
WP_001295297.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73900; AAC73900; b0813.
BAA35485; BAA35485; BAA35485.
GeneIDi947045.
KEGGiecj:JW0798.
eco:b0813.
PATRICi32116829. VBIEscCol129921_0840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04242 Genomic DNA. Translation: AAA21854.1.
U00096 Genomic DNA. Translation: AAC73900.1.
AP009048 Genomic DNA. Translation: BAA35485.1.
PIRiE64818.
RefSeqiNP_415334.1. NC_000913.3.
WP_001295297.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AA67.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259985. 13 interactions.
STRINGi511145.b0813.

Protein family/group databases

TCDBi2.A.7.3.6. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PaxDbiP0AA67.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73900; AAC73900; b0813.
BAA35485; BAA35485; BAA35485.
GeneIDi947045.
KEGGiecj:JW0798.
eco:b0813.
PATRICi32116829. VBIEscCol129921_0840.

Organism-specific databases

EchoBASEiEB2055.
EcoGeneiEG12134. rhtA.

Phylogenomic databases

eggNOGiENOG4107AQZ. Bacteria.
COG5006. LUCA.
HOGENOMiHOG000283695.
InParanoidiP0AA67.
KOiK11939.
OMAiMALGGMN.
OrthoDBiEOG67T5NC.
PhylomeDBiP0AA67.

Enzyme and pathway databases

BioCyciEcoCyc:EG12134-MONOMER.
ECOL316407:JW0798-MONOMER.
MetaCyc:EG12134-MONOMER.
RETL1328306-WGS:GSTH-257-MONOMER.

Miscellaneous databases

PROiP0AA67.

Family and domain databases

InterProiIPR000620. EamA_dom.
[Graphical view]
PfamiPF00892. EamA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the sigma 38-dependent expression of a core Escherichia coli starvation gene, pexB."
    Lomovskaya O.L., Kidwell J.P., Matin A.
    J. Bacteriol. 176:3928-3935(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification and characterization of the new gene rhtA involved in threonine and homoserine efflux in Escherichia coli."
    Livshits V.A., Zakataeva N.P., Aleshin V.V., Vitushkina M.V.
    Res. Microbiol. 154:123-135(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiRHTA_ECOLI
AccessioniPrimary (citable) accession number: P0AA67
Secondary accession number(s): P36545, P75784
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: January 20, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.