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Protein

Inner membrane protein YghB

Gene

yghB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be a membrane transporter required for proton motive force (PMF)-dependent drug efflux. Required, with YqjA, for the proper export of certain periplasmic amidases and, possibly, other Tat substrates. May play a role in determining membrane lipid composition.3 Publications

GO - Molecular functioni

  • transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • FtsZ-dependent cytokinesis Source: EcoCyc
  • transmembrane transport Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG11824-MONOMER.
ECOL316407:JW2976-MONOMER.

Protein family/group databases

TCDBi9.B.27.2.1. the deda or ydjx-z (deda) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Inner membrane protein YghB
Gene namesi
Name:yghB
Ordered Locus Names:b3009, JW2976
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11824. yghB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1717CytoplasmicSequence analysisAdd
BLAST
Transmembranei18 – 3821HelicalSequence analysisAdd
BLAST
Topological domaini39 – 6729PeriplasmicSequence analysisAdd
BLAST
Transmembranei68 – 8821HelicalSequence analysisAdd
BLAST
Topological domaini89 – 16072CytoplasmicSequence analysisAdd
BLAST
Transmembranei161 – 18121HelicalSequence analysisAdd
BLAST
Topological domaini182 – 19110PeriplasmicSequence analysis
Transmembranei192 – 21221HelicalSequence analysisAdd
BLAST
Topological domaini213 – 2197CytoplasmicSequence analysis

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Double mutants lacking both yghB and yqjA show incomplete cell division, temperature sensitivity and altered phospholipid levels. They are also hypersensitive to several compounds known to be exported by other drug efflux proteins, including ethidium bromide, methyl viologen, acriflavine and beta-lactam antibiotics. Expression of either yghB or yqjA can restore the wild-type phenotype, suggesting that these proteins have redundant functions. Both individual null mutant strains grow normally at all temperatures.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi39 – 391E → A: Abolishes the ability to restore growth, cell division or drug resistance in double mutant. 1 Publication
Mutagenesisi51 – 511D → A: Abolishes the ability to restore growth, cell division or drug resistance in double mutant. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 219219Inner membrane protein YghBPRO_0000161411Add
BLAST

Proteomic databases

PaxDbiP0AA60.

Interactioni

Protein-protein interaction databases

BioGridi4262378. 169 interactions.
STRINGi511145.b3009.

Structurei

3D structure databases

ProteinModelPortaliP0AA60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DedA family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108K81. Bacteria.
COG0586. LUCA.
HOGENOMiHOG000105367.
InParanoidiP0AA60.
OMAiASLLMYW.
PhylomeDBiP0AA60.

Family and domain databases

InterProiIPR032818. DedA.
IPR032816. SNARE_assoc.
[Graphical view]
PANTHERiPTHR30353. PTHR30353. 1 hit.
PfamiPF09335. SNARE_assoc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AA60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVIQDIIAA LWQHDFAALA DPHIVSVVYF VMFATLFLEN GLLPASFLPG
60 70 80 90 100
DSLLILAGAL IAQGVMDFLP TIAILTAAAS LGCWLSYIQG RWLGNTKTVK
110 120 130 140 150
GWLAQLPAKY HQRATCMFDR HGLLALLAGR FLAFVRTLLP TMAGISGLPN
160 170 180 190 200
RRFQFFNWLS GLLWVSVVTS FGYALSMIPF VKRHEDQVMT FLMILPIALL
210
TAGLLGTLFV VIKKKYCNA
Length:219
Mass (Da):24,134
Last modified:September 13, 2005 - v1
Checksum:i726BDC4B7194B473
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69176.1.
U00096 Genomic DNA. Translation: AAC76045.1.
AP009048 Genomic DNA. Translation: BAE77066.1.
M12858 Genomic DNA. No translation available.
PIRiG65087.
RefSeqiNP_417482.1. NC_000913.3.
WP_000268419.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76045; AAC76045; b3009.
BAE77066; BAE77066; BAE77066.
GeneIDi947490.
KEGGiecj:JW2976.
eco:b3009.
PATRICi32121432. VBIEscCol129921_3103.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69176.1.
U00096 Genomic DNA. Translation: AAC76045.1.
AP009048 Genomic DNA. Translation: BAE77066.1.
M12858 Genomic DNA. No translation available.
PIRiG65087.
RefSeqiNP_417482.1. NC_000913.3.
WP_000268419.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AA60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262378. 169 interactions.
STRINGi511145.b3009.

Protein family/group databases

TCDBi9.B.27.2.1. the deda or ydjx-z (deda) family.

Proteomic databases

PaxDbiP0AA60.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76045; AAC76045; b3009.
BAE77066; BAE77066; BAE77066.
GeneIDi947490.
KEGGiecj:JW2976.
eco:b3009.
PATRICi32121432. VBIEscCol129921_3103.

Organism-specific databases

EchoBASEiEB1771.
EcoGeneiEG11824. yghB.

Phylogenomic databases

eggNOGiENOG4108K81. Bacteria.
COG0586. LUCA.
HOGENOMiHOG000105367.
InParanoidiP0AA60.
OMAiASLLMYW.
PhylomeDBiP0AA60.

Enzyme and pathway databases

BioCyciEcoCyc:EG11824-MONOMER.
ECOL316407:JW2976-MONOMER.

Miscellaneous databases

PROiP0AA60.

Family and domain databases

InterProiIPR032818. DedA.
IPR032816. SNARE_assoc.
[Graphical view]
PANTHERiPTHR30353. PTHR30353. 1 hit.
PfamiPF09335. SNARE_assoc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYGHB_ECOLI
AccessioniPrimary (citable) accession number: P0AA60
Secondary accession number(s): P33196, Q2M9J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.