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Protein

Low-affinity putrescine importer PlaP

Gene

plaP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putrescine importer. Required for induction of type 1 pili-driven surface motility.1 Publication

Kineticsi

  1. KM=155 µM for putrescine1 Publication
  1. Vmax=9.3 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

  • putrescine transmembrane transporter activity Source: EcoCyc
  • solute:proton symporter activity Source: EcoCyc

GO - Biological processi

  • amino acid transport Source: UniProtKB-KW
  • cell motility Source: EcoCyc
  • putrescine transport Source: EcoCyc

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YEEF-MONOMER
MetaCyc:YEEF-MONOMER

Protein family/group databases

TCDBi2.A.3.1.14 the amino acid-polyamine-organocation (apc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity putrescine importer PlaP
Gene namesi
Name:plaP
Synonyms:yeeF
Ordered Locus Names:b2014, JW5330
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11896 plaP

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Topological domaini38 – 48PeriplasmicSequence analysisAdd BLAST11
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 95CytoplasmicSequence analysisAdd BLAST26
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 123PeriplasmicSequence analysis7
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 158CytoplasmicSequence analysisAdd BLAST14
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Topological domaini180 – 199PeriplasmicSequence analysisAdd BLAST20
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
Topological domaini221 – 237CytoplasmicSequence analysisAdd BLAST17
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 283PeriplasmicSequence analysisAdd BLAST25
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 339CytoplasmicSequence analysisAdd BLAST35
Transmembranei340 – 360HelicalSequence analysisAdd BLAST21
Transmembranei361 – 381HelicalSequence analysisAdd BLAST21
Topological domaini382 – 394CytoplasmicSequence analysisAdd BLAST13
Transmembranei395 – 415HelicalSequence analysisAdd BLAST21
Topological domaini416 – 417PeriplasmicSequence analysis2
Transmembranei418 – 438HelicalSequence analysisAdd BLAST21
Topological domaini439 – 452CytoplasmicSequence analysisAdd BLAST14

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542151 – 452Low-affinity putrescine importer PlaPAdd BLAST452

Proteomic databases

PaxDbiP0AA47
PRIDEiP0AA47

Interactioni

Protein-protein interaction databases

BioGridi4260417, 173 interactors
IntActiP0AA47, 1 interactor
STRINGi316385.ECDH10B_2161

Structurei

3D structure databases

ProteinModelPortaliP0AA47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CUB Bacteria
COG0531 LUCA
HOGENOMiHOG000164060
InParanoidiP0AA47
OMAiTIKWIVT
PhylomeDBiP0AA47

Family and domain databases

InterProiView protein in InterPro
IPR002293 AA/rel_permease1
PfamiView protein in Pfam
PF13520 AA_permease_2, 1 hit
PIRSFiPIRSF006060 AA_transporter, 1 hit

Sequencei

Sequence statusi: Complete.

P0AA47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHNVTPNTS RVELRKTLTL VPVVMMGLAY MQPMTLFDTF GIVSGLTDGH
60 70 80 90 100
VPTAYAFALI AILFTALSYG KLVRRYPSAG SAYTYAQKSI SPTVGFMVGW
110 120 130 140 150
SSLLDYLFAP MINILLAKIY FEALVPSIPS WMFVVALVAF MTAFNLRSLK
160 170 180 190 200
SVANFNTVIV VLQVVLIAVI LGMVVYGVFE GEGAGTLAST RPFWSGDAHV
210 220 230 240 250
IPMITGATIL CFSFTGFDGI SNLSEETKDA ERVIPRAIFL TALIGGMIFI
260 270 280 290 300
FATYFLQLYF PDISRFKDPD ASQPEIMLYV AGKAFQVGAL IFSTITVLAS
310 320 330 340 350
GMAAHAGVAR LMYVMGRDGV FPKSFFGYVH PKWRTPAMNI ILVGAIALLA
360 370 380 390 400
INFDLVMATA LINFGALVAF TFVNLSVISQ FWIREKRNKT LKDHFQYLFL
410 420 430 440 450
PMCGALTVGA LWVNLEESSM VLGLIWAAIG LIYLACVTKS FRNPVPQYED

VA
Length:452
Mass (Da):49,538
Last modified:September 13, 2005 - v1
Checksum:iF8B618C6BD3E762E
GO

Sequence cautioni

The sequence AAA16420 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00009 Genomic DNA Translation: AAA16420.1 Different initiation.
U00096 Genomic DNA Translation: AAC75075.2
AP009048 Genomic DNA Translation: BAA15842.2
PIRiE64966
RefSeqiNP_416518.2, NC_000913.3
WP_000019197.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75075; AAC75075; b2014
BAA15842; BAA15842; BAA15842
GeneIDi946533
KEGGiecj:JW5330
eco:b2014
PATRICifig|1411691.4.peg.238

Entry informationi

Entry nameiPLAP_ECOLI
AccessioniPrimary (citable) accession number: P0AA47
Secondary accession number(s): P33016
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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