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Protein

Low-affinity putrescine importer PlaP

Gene

plaP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putrescine importer. Required for induction of type 1 pili-driven surface motility.1 Publication

Kineticsi

  1. KM=155 µM for putrescine1 Publication
  1. Vmax=9.3 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

  • antiporter activity Source: GO_Central
  • L-amino acid transmembrane transporter activity Source: GO_Central
  • putrescine transmembrane transporter activity Source: EcoCyc
  • solute:proton symporter activity Source: EcoCyc

GO - Biological processi

  • cell motility Source: EcoCyc
  • putrescine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YEEF-MONOMER.
ECOL316407:JW5330-MONOMER.
MetaCyc:YEEF-MONOMER.

Protein family/group databases

TCDBi2.A.3.1.14. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity putrescine importer PlaP
Gene namesi
Name:plaP
Synonyms:yeeF
Ordered Locus Names:b2014, JW5330
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11896. plaP.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence analysisAdd
BLAST
Transmembranei17 – 3721HelicalSequence analysisAdd
BLAST
Topological domaini38 – 4811PeriplasmicSequence analysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence analysisAdd
BLAST
Topological domaini70 – 9526CytoplasmicSequence analysisAdd
BLAST
Transmembranei96 – 11621HelicalSequence analysisAdd
BLAST
Topological domaini117 – 1237PeriplasmicSequence analysis
Transmembranei124 – 14421HelicalSequence analysisAdd
BLAST
Topological domaini145 – 15814CytoplasmicSequence analysisAdd
BLAST
Transmembranei159 – 17921HelicalSequence analysisAdd
BLAST
Topological domaini180 – 19920PeriplasmicSequence analysisAdd
BLAST
Transmembranei200 – 22021HelicalSequence analysisAdd
BLAST
Topological domaini221 – 23717CytoplasmicSequence analysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Topological domaini259 – 28325PeriplasmicSequence analysisAdd
BLAST
Transmembranei284 – 30421HelicalSequence analysisAdd
BLAST
Topological domaini305 – 33935CytoplasmicSequence analysisAdd
BLAST
Transmembranei340 – 36021HelicalSequence analysisAdd
BLAST
Transmembranei361 – 38121HelicalSequence analysisAdd
BLAST
Topological domaini382 – 39413CytoplasmicSequence analysisAdd
BLAST
Transmembranei395 – 41521HelicalSequence analysisAdd
BLAST
Topological domaini416 – 4172PeriplasmicSequence analysis
Transmembranei418 – 43821HelicalSequence analysisAdd
BLAST
Topological domaini439 – 45214CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Low-affinity putrescine importer PlaPPRO_0000054215Add
BLAST

Proteomic databases

PaxDbiP0AA47.

Interactioni

Protein-protein interaction databases

BioGridi4260417. 152 interactions.
IntActiP0AA47. 1 interaction.
STRINGi511145.b2014.

Structurei

3D structure databases

ProteinModelPortaliP0AA47.
SMRiP0AA47. Positions 16-376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CUB. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164060.
InParanoidiP0AA47.
OMAiPAVGFMV.
PhylomeDBiP0AA47.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AA47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHNVTPNTS RVELRKTLTL VPVVMMGLAY MQPMTLFDTF GIVSGLTDGH
60 70 80 90 100
VPTAYAFALI AILFTALSYG KLVRRYPSAG SAYTYAQKSI SPTVGFMVGW
110 120 130 140 150
SSLLDYLFAP MINILLAKIY FEALVPSIPS WMFVVALVAF MTAFNLRSLK
160 170 180 190 200
SVANFNTVIV VLQVVLIAVI LGMVVYGVFE GEGAGTLAST RPFWSGDAHV
210 220 230 240 250
IPMITGATIL CFSFTGFDGI SNLSEETKDA ERVIPRAIFL TALIGGMIFI
260 270 280 290 300
FATYFLQLYF PDISRFKDPD ASQPEIMLYV AGKAFQVGAL IFSTITVLAS
310 320 330 340 350
GMAAHAGVAR LMYVMGRDGV FPKSFFGYVH PKWRTPAMNI ILVGAIALLA
360 370 380 390 400
INFDLVMATA LINFGALVAF TFVNLSVISQ FWIREKRNKT LKDHFQYLFL
410 420 430 440 450
PMCGALTVGA LWVNLEESSM VLGLIWAAIG LIYLACVTKS FRNPVPQYED

VA
Length:452
Mass (Da):49,538
Last modified:September 13, 2005 - v1
Checksum:iF8B618C6BD3E762E
GO

Sequence cautioni

The sequence AAA16420 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00009 Genomic DNA. Translation: AAA16420.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75075.2.
AP009048 Genomic DNA. Translation: BAA15842.2.
PIRiE64966.
RefSeqiNP_416518.2. NC_000913.3.
WP_000019197.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75075; AAC75075; b2014.
BAA15842; BAA15842; BAA15842.
GeneIDi946533.
KEGGiecj:JW5330.
eco:b2014.
PATRICi32119363. VBIEscCol129921_2091.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00009 Genomic DNA. Translation: AAA16420.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75075.2.
AP009048 Genomic DNA. Translation: BAA15842.2.
PIRiE64966.
RefSeqiNP_416518.2. NC_000913.3.
WP_000019197.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AA47.
SMRiP0AA47. Positions 16-376.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260417. 152 interactions.
IntActiP0AA47. 1 interaction.
STRINGi511145.b2014.

Protein family/group databases

TCDBi2.A.3.1.14. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbiP0AA47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75075; AAC75075; b2014.
BAA15842; BAA15842; BAA15842.
GeneIDi946533.
KEGGiecj:JW5330.
eco:b2014.
PATRICi32119363. VBIEscCol129921_2091.

Organism-specific databases

EchoBASEiEB1842.
EcoGeneiEG11896. plaP.

Phylogenomic databases

eggNOGiENOG4105CUB. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164060.
InParanoidiP0AA47.
OMAiPAVGFMV.
PhylomeDBiP0AA47.

Enzyme and pathway databases

BioCyciEcoCyc:YEEF-MONOMER.
ECOL316407:JW5330-MONOMER.
MetaCyc:YEEF-MONOMER.

Miscellaneous databases

PROiP0AA47.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLAP_ECOLI
AccessioniPrimary (citable) accession number: P0AA47
Secondary accession number(s): P33016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.