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Protein

Low-affinity putrescine importer PlaP

Gene

plaP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putrescine importer. Required for induction of type 1 pili-driven surface motility.1 Publication

Kineticsi

  1. KM=155 µM for putrescine1 Publication
  1. Vmax=9.3 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

  • antiporter activity Source: GO_Central
  • L-amino acid transmembrane transporter activity Source: GO_Central
  • putrescine transmembrane transporter activity Source: EcoCyc
  • solute:proton symporter activity Source: EcoCyc

GO - Biological processi

  • cell motility Source: EcoCyc
  • putrescine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YEEF-MONOMER.
ECOL316407:JW5330-MONOMER.
MetaCyc:YEEF-MONOMER.

Protein family/group databases

TCDBi2.A.3.1.14. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity putrescine importer PlaP
Gene namesi
Name:plaP
Synonyms:yeeF
Ordered Locus Names:b2014, JW5330
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11896. plaP.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Topological domaini38 – 48PeriplasmicSequence analysisAdd BLAST11
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 95CytoplasmicSequence analysisAdd BLAST26
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 123PeriplasmicSequence analysis7
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 158CytoplasmicSequence analysisAdd BLAST14
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Topological domaini180 – 199PeriplasmicSequence analysisAdd BLAST20
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
Topological domaini221 – 237CytoplasmicSequence analysisAdd BLAST17
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 283PeriplasmicSequence analysisAdd BLAST25
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 339CytoplasmicSequence analysisAdd BLAST35
Transmembranei340 – 360HelicalSequence analysisAdd BLAST21
Transmembranei361 – 381HelicalSequence analysisAdd BLAST21
Topological domaini382 – 394CytoplasmicSequence analysisAdd BLAST13
Transmembranei395 – 415HelicalSequence analysisAdd BLAST21
Topological domaini416 – 417PeriplasmicSequence analysis2
Transmembranei418 – 438HelicalSequence analysisAdd BLAST21
Topological domaini439 – 452CytoplasmicSequence analysisAdd BLAST14

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542151 – 452Low-affinity putrescine importer PlaPAdd BLAST452

Proteomic databases

PaxDbiP0AA47.
PRIDEiP0AA47.

Interactioni

Protein-protein interaction databases

BioGridi4260417. 152 interactors.
IntActiP0AA47. 1 interactor.
STRINGi511145.b2014.

Structurei

3D structure databases

ProteinModelPortaliP0AA47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CUB. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164060.
InParanoidiP0AA47.
OMAiPAVGFMV.
PhylomeDBiP0AA47.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AA47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHNVTPNTS RVELRKTLTL VPVVMMGLAY MQPMTLFDTF GIVSGLTDGH
60 70 80 90 100
VPTAYAFALI AILFTALSYG KLVRRYPSAG SAYTYAQKSI SPTVGFMVGW
110 120 130 140 150
SSLLDYLFAP MINILLAKIY FEALVPSIPS WMFVVALVAF MTAFNLRSLK
160 170 180 190 200
SVANFNTVIV VLQVVLIAVI LGMVVYGVFE GEGAGTLAST RPFWSGDAHV
210 220 230 240 250
IPMITGATIL CFSFTGFDGI SNLSEETKDA ERVIPRAIFL TALIGGMIFI
260 270 280 290 300
FATYFLQLYF PDISRFKDPD ASQPEIMLYV AGKAFQVGAL IFSTITVLAS
310 320 330 340 350
GMAAHAGVAR LMYVMGRDGV FPKSFFGYVH PKWRTPAMNI ILVGAIALLA
360 370 380 390 400
INFDLVMATA LINFGALVAF TFVNLSVISQ FWIREKRNKT LKDHFQYLFL
410 420 430 440 450
PMCGALTVGA LWVNLEESSM VLGLIWAAIG LIYLACVTKS FRNPVPQYED

VA
Length:452
Mass (Da):49,538
Last modified:September 13, 2005 - v1
Checksum:iF8B618C6BD3E762E
GO

Sequence cautioni

The sequence AAA16420 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00009 Genomic DNA. Translation: AAA16420.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75075.2.
AP009048 Genomic DNA. Translation: BAA15842.2.
PIRiE64966.
RefSeqiNP_416518.2. NC_000913.3.
WP_000019197.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75075; AAC75075; b2014.
BAA15842; BAA15842; BAA15842.
GeneIDi946533.
KEGGiecj:JW5330.
eco:b2014.
PATRICi32119363. VBIEscCol129921_2091.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00009 Genomic DNA. Translation: AAA16420.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75075.2.
AP009048 Genomic DNA. Translation: BAA15842.2.
PIRiE64966.
RefSeqiNP_416518.2. NC_000913.3.
WP_000019197.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AA47.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260417. 152 interactors.
IntActiP0AA47. 1 interactor.
STRINGi511145.b2014.

Protein family/group databases

TCDBi2.A.3.1.14. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbiP0AA47.
PRIDEiP0AA47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75075; AAC75075; b2014.
BAA15842; BAA15842; BAA15842.
GeneIDi946533.
KEGGiecj:JW5330.
eco:b2014.
PATRICi32119363. VBIEscCol129921_2091.

Organism-specific databases

EchoBASEiEB1842.
EcoGeneiEG11896. plaP.

Phylogenomic databases

eggNOGiENOG4105CUB. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164060.
InParanoidiP0AA47.
OMAiPAVGFMV.
PhylomeDBiP0AA47.

Enzyme and pathway databases

BioCyciEcoCyc:YEEF-MONOMER.
ECOL316407:JW5330-MONOMER.
MetaCyc:YEEF-MONOMER.

Miscellaneous databases

PROiP0AA47.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLAP_ECOLI
AccessioniPrimary (citable) accession number: P0AA47
Secondary accession number(s): P33016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.