P0AA46 (RSUA_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal small subunit pseudouridine synthase A
EC=5.4.99.19

Alternative name(s):
16S pseudouridylate 516 synthase
16S rRNA pseudouridine(516) synthase
rRNA pseudouridylate synthase A
rRNA-uridine isomerase A

Gene names

Name:	rsuA
Ordered Locus Names:	SF2270, S2399

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-516 in 16S ribosomal RNA By similarity.
Catalytic activity	16S rRNA uridine(516) = 16S rRNA pseudouridine(516).
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the pseudouridine synthase RsuA family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	RNA-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Ribosomal small subunit pseudouridine synthase A		PRO_0000099974
Regions
	Domain	1 – 68	68	S4 RNA-binding
Sites
	Active site	102	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AA46 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 04EB7154931C58F7	FASTA	231	25,865
	10 20 30 40 50 60 MRLDKFIAQQ LGVSRAIAGR EIRGNRVTVD GEIVRNAAFK LLPEHDVAYD GNPLAQQHGP 70 80 90 100 110 120 RYFMLNKPQG YVCSTDDPDH PTVLYFLDEP VAWKLHAAGR LDIDTTGLVL MTDDGQWSHR 130 140 150 160 170 180 ITSPRHHCEK TYLVTLESPV ADDTAEQFAK GVQLHNEKDL TKPAVLEVIT PTQVRLTISE 190 200 210 220 230 GRYHQVKRMF AAVGNHVVEL HRERIGGITL DADLAPGEYR PLTEEEIASV V

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN43789.1. AE014073 Genomic DNA. Translation: AAP17606.1.
RefSeq	NP_708082.1. NC_004337.2. NP_837797.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0AA46.
SMR	P0AA46. Positions 1-231.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF2270.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN43789; AAN43789; SF2270. AAP17606; AAP17606; S2399.
GeneID	1024633. 1078689.
KEGG	sfl:SF2270. sfx:S2399.
PATRIC	18706367. VBIShiFle31049_2660.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1187.
HOGENOM	HOG000044954.
KO	K06183.
OMA	IGPRFIM.
ProtClustDB	PRK10839.
Family and domain databases
Gene3D	3.10.290.10. 1 hit.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR000748. PsdUridine_synth_RsuA/RluB/E/F. IPR018496. PsdUridine_synth_RsuA/RluB_CS. IPR006145. PsdUridine_synth_RsuA/RluD. IPR002942. S4_RNA-bd. [Graphical view]
Pfam	PF00849. PseudoU_synth_2. 1 hit. PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00093. TIGR00093. 1 hit.
PROSITE	PS01149. PSI_RSU. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSUA_SHIFL

Accession

Primary (citable) accession number: P0AA46
Secondary accession number(s): P33918

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program