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Protein

Ribosomal small subunit pseudouridine synthase A

Gene

rsuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-516 in 16S ribosomal RNA.2 Publications

Catalytic activityi

16S rRNA uridine(516) = 16S rRNA pseudouridine(516).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102Nucleophile1 Publication1

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc

Keywordsi

Molecular functionIsomerase, RNA-binding
Biological processrRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG12044-MONOMER
MetaCyc:EG12044-MONOMER
BRENDAi5.4.99.19 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal small subunit pseudouridine synthase A (EC:5.4.99.19)
Alternative name(s):
16S pseudouridylate 516 synthase
16S rRNA pseudouridine(516) synthase
rRNA pseudouridylate synthase A
rRNA-uridine isomerase A
Gene namesi
Name:rsuA
Synonyms:yejD
Ordered Locus Names:b2183, JW2171
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12044 rsuA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi102D → N or T: Loss of activity. 1 Publication1

Chemistry databases

DrugBankiDB03419 Uracil
DB03685 Uridine-5'-Monophosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000999661 – 231Ribosomal small subunit pseudouridine synthase AAdd BLAST231

Proteomic databases

PaxDbiP0AA43
PRIDEiP0AA43

2D gel databases

SWISS-2DPAGEP0AA43

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
rpsJP0A7R53EBI-557810,EBI-544602

Protein-protein interaction databases

BioGridi4260474, 48 interactors
DIPiDIP-35902N
IntActiP0AA43, 40 interactors
STRINGi316385.ECDH10B_2341

Structurei

Secondary structure

1231
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 11Combined sources9
Helixi15 – 23Combined sources9
Turni24 – 26Combined sources3
Beta strandi27 – 29Combined sources3
Beta strandi47 – 49Combined sources3
Beta strandi52 – 54Combined sources3
Beta strandi62 – 67Combined sources6
Beta strandi72 – 76Combined sources5
Beta strandi78 – 80Combined sources3
Helixi83 – 86Combined sources4
Helixi92 – 94Combined sources3
Beta strandi96 – 99Combined sources4
Beta strandi106 – 113Combined sources8
Helixi115 – 122Combined sources8
Beta strandi130 – 138Combined sources9
Helixi144 – 150Combined sources7
Beta strandi165 – 170Combined sources6
Beta strandi173 – 179Combined sources7
Helixi185 – 192Combined sources8
Beta strandi197 – 205Combined sources9
Beta strandi219 – 221Combined sources3
Helixi224 – 227Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KSKX-ray2.00A1-231[»]
1KSLX-ray2.10A1-231[»]
1KSVX-ray2.65A1-231[»]
ProteinModelPortaliP0AA43
SMRiP0AA43
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AA43

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 68S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST68

Sequence similaritiesi

Belongs to the pseudouridine synthase RsuA family.Curated

Phylogenomic databases

eggNOGiENOG4105I08 Bacteria
COG1187 LUCA
HOGENOMiHOG000044954
InParanoidiP0AA43
KOiK06183
OMAiSQGFGTR
PhylomeDBiP0AA43

Family and domain databases

CDDicd00165 S4, 1 hit
Gene3Di3.10.290.10, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR000748 PsdUridine_synth_RsuA/RluB/E/F
IPR018496 PsdUridine_synth_RsuA/RluB_CS
IPR006145 PsdUridine_synth_RsuA/RluD
IPR002942 S4_RNA-bd
IPR036986 S4_RNA-bd_sf
PANTHERiPTHR21600 PTHR21600, 1 hit
PfamiView protein in Pfam
PF00849 PseudoU_synth_2, 1 hit
PF01479 S4, 1 hit
SMARTiView protein in SMART
SM00363 S4, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
TIGRFAMsiTIGR00093 TIGR00093, 1 hit
PROSITEiView protein in PROSITE
PS01149 PSI_RSU, 1 hit
PS50889 S4, 1 hit

Sequencei

Sequence statusi: Complete.

P0AA43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLDKFIAQQ LGVSRAIAGR EIRGNRVTVD GEIVRNAAFK LLPEHDVAYD
60 70 80 90 100
GNPLAQQHGP RYFMLNKPQG YVCSTDDPDH PTVLYFLDEP VAWKLHAAGR
110 120 130 140 150
LDIDTTGLVL MTDDGQWSHR ITSPRHHCEK TYLVTLESPV ADDTAEQFAK
160 170 180 190 200
GVQLHNEKDL TKPAVLEVIT PTQVRLTISE GRYHQVKRMF AAVGNHVVEL
210 220 230
HRERIGGITL DADLAPGEYR PLTEEEIASV V
Length:231
Mass (Da):25,865
Last modified:September 13, 2005 - v1
Checksum:i04EB7154931C58F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA Translation: AAA16377.1
U00096 Genomic DNA Translation: AAC75244.1
AP009048 Genomic DNA Translation: BAE76648.1
PIRiF64987
RefSeqiNP_416688.1, NC_000913.3
WP_001234850.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75244; AAC75244; b2183
BAE76648; BAE76648; BAE76648
GeneIDi945378
KEGGiecj:JW2171
eco:b2183
PATRICifig|1411691.4.peg.53

Similar proteinsi

Entry informationi

Entry nameiRSUA_ECOLI
AccessioniPrimary (citable) accession number: P0AA43
Secondary accession number(s): P33918, Q2MAQ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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