Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosomal large subunit pseudouridine synthase C

Gene

rluC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil at positions 955, 2504 and 2580 in 23S ribosomal RNA.

Catalytic activityi

23S rRNA uridine(955)/uridine(2504)/uridine(2580) = 23S rRNA pseudouridine(955)/pseudouridine(2504)/pseudouridine(2580).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei144 – 1441By similarity

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11118-MONOMER.
ECOL316407:JW1072-MONOMER.
MetaCyc:EG11118-MONOMER.
BRENDAi5.4.99.24. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal large subunit pseudouridine synthase C (EC:5.4.99.24)
Alternative name(s):
23S rRNA pseudouridine(955/2504/2580) synthase
rRNA pseudouridylate synthase C
rRNA-uridine isomerase C
Gene namesi
Name:rluC
Synonyms:yceC
Ordered Locus Names:b1086, JW1072
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11118. rluC.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Ribosomal large subunit pseudouridine synthase CPRO_0000162667Add
BLAST

Proteomic databases

PaxDbiP0AA39.
PRIDEiP0AA39.

Interactioni

Protein-protein interaction databases

BioGridi4261025. 9 interactions.
850009. 1 interaction.
DIPiDIP-47832N.
IntActiP0AA39. 70 interactions.
STRINGi511145.b1086.

Structurei

Secondary structure

1
319
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi91 – 944Combined sources
Beta strandi95 – 984Combined sources
Beta strandi100 – 1078Combined sources
Beta strandi117 – 1204Combined sources
Helixi123 – 1308Combined sources
Beta strandi138 – 1414Combined sources
Beta strandi148 – 1569Combined sources
Helixi157 – 16812Combined sources
Beta strandi172 – 18211Combined sources
Beta strandi195 – 1995Combined sources
Beta strandi205 – 2095Combined sources
Beta strandi218 – 2258Combined sources
Beta strandi227 – 23812Combined sources
Helixi243 – 2508Combined sources
Turni259 – 2613Combined sources
Helixi264 – 2729Combined sources
Beta strandi282 – 2909Combined sources
Turni292 – 2943Combined sources
Beta strandi297 – 3015Combined sources
Helixi306 – 31712Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V9KX-ray2.00A/B92-319[»]
1XPIX-ray2.20A/B89-319[»]
ProteinModelPortaliP0AA39.
SMRiP0AA39. Positions 13-317.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AA39.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 8364S4 RNA-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pseudouridine synthase RluA family.Curated
Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105EI2. Bacteria.
COG0564. LUCA.
HOGENOMiHOG000275914.
InParanoidiP0AA39.
KOiK06179.
OMAiEAPMDEQ.
OrthoDBiEOG6P070X.
PhylomeDBiP0AA39.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR006225. PsdUridine_synth_RluC/D.
IPR006224. PsdUridine_synth_RluC/D_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00005. rluA_subfam. 1 hit.
PROSITEiPS01129. PSI_RLU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AA39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTETPSVKI VAITADEAGQ RIDNFLRTQL KGVPKSMIYR ILRKGEVRVN
60 70 80 90 100
KKRIKPEYKL EAGDEVRIPP VRVAEREEEA VSPHLQKVAA LADVILYEDD
110 120 130 140 150
HILVLNKPSG TAVHGGSGLS FGVIEGLRAL RPEARFLELV HRLDRDTSGV
160 170 180 190 200
LLVAKKRSAL RSLHEQLREK GMQKDYLALV RGQWQSHVKS VQAPLLKNIL
210 220 230 240 250
QSGERIVRVS QEGKPSETRF KVEERYAFAT LVRCSPVTGR THQIRVHTQY
260 270 280 290 300
AGHPIAFDDR YGDREFDRQL TEAGTGLNRL FLHAAALKFT HPGTGEVMRI
310
EAPMDEGLKR CLQKLRNAR
Length:319
Mass (Da):36,027
Last modified:September 13, 2005 - v1
Checksum:i19C8D05E426F0344
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74170.1.
AP009048 Genomic DNA. Translation: BAA35894.1.
M62747 Genomic DNA. Translation: AAA23442.1.
M96791 Genomic DNA. Translation: AAA23828.1.
PIRiC64852.
RefSeqiNP_415604.1. NC_000913.3.
WP_000846343.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74170; AAC74170; b1086.
BAA35894; BAA35894; BAA35894.
GeneIDi945637.
KEGGiecj:JW1072.
eco:b1086.
PATRICi32117413. VBIEscCol129921_1129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74170.1.
AP009048 Genomic DNA. Translation: BAA35894.1.
M62747 Genomic DNA. Translation: AAA23442.1.
M96791 Genomic DNA. Translation: AAA23828.1.
PIRiC64852.
RefSeqiNP_415604.1. NC_000913.3.
WP_000846343.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V9KX-ray2.00A/B92-319[»]
1XPIX-ray2.20A/B89-319[»]
ProteinModelPortaliP0AA39.
SMRiP0AA39. Positions 13-317.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261025. 9 interactions.
850009. 1 interaction.
DIPiDIP-47832N.
IntActiP0AA39. 70 interactions.
STRINGi511145.b1086.

Proteomic databases

PaxDbiP0AA39.
PRIDEiP0AA39.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74170; AAC74170; b1086.
BAA35894; BAA35894; BAA35894.
GeneIDi945637.
KEGGiecj:JW1072.
eco:b1086.
PATRICi32117413. VBIEscCol129921_1129.

Organism-specific databases

EchoBASEiEB1108.
EcoGeneiEG11118. rluC.

Phylogenomic databases

eggNOGiENOG4105EI2. Bacteria.
COG0564. LUCA.
HOGENOMiHOG000275914.
InParanoidiP0AA39.
KOiK06179.
OMAiEAPMDEQ.
OrthoDBiEOG6P070X.
PhylomeDBiP0AA39.

Enzyme and pathway databases

BioCyciEcoCyc:EG11118-MONOMER.
ECOL316407:JW1072-MONOMER.
MetaCyc:EG11118-MONOMER.
BRENDAi5.4.99.24. 2026.

Miscellaneous databases

EvolutionaryTraceiP0AA39.
PROiP0AA39.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR006225. PsdUridine_synth_RluC/D.
IPR006224. PsdUridine_synth_RluC/D_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00005. rluA_subfam. 1 hit.
PROSITEiPS01129. PSI_RLU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Analysis of the altered mRNA stability (ams) gene from Escherichia coli. Nucleotide sequence, transcriptional analysis, and homology of its product to MRP3, a mitochondrial ribosomal protein from Neurospora crassa."
    Claverie-Martin F., Diaz-Torres M., Yancey S.D., Kushner S.R.
    J. Biol. Chem. 266:2843-2851(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-285.
    Strain: K12.
  5. "Physical locations of genes in the rne (ams)-rpmF-plsX-fab region of the Escherichia coli K-12 chromosome."
    Oh W., Larson T.J.
    J. Bacteriol. 174:7873-7874(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 260-319.
    Strain: K12.
  6. "The rluC gene of Escherichia coli codes for a pseudouridine synthase that is solely responsible for synthesis of pseudouridine at positions 955, 2504, and 2580 in 23 S ribosomal RNA."
    Conrad J., Sun D., Englund N., Ofengand J.
    J. Biol. Chem. 273:18562-18566(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
  7. "Crystallization and characterization of a fragment of pseudouridine synthase RluC from Escherichia coli."
    Corollo D., Blair-Johnson M., Conrad J., Fiedler T., Sun D., Wang L., Ofengand J., Fenna R.
    Acta Crystallogr. D 55:302-304(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION OF 89-319, PROTEIN SEQUENCE OF 89-98.
    Strain: BL21-DE3.

Entry informationi

Entry nameiRLUC_ECOLI
AccessioniPrimary (citable) accession number: P0AA39
Secondary accession number(s): P23851
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: January 20, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.