Reviewed, UniProtKB/Swiss-Prot P0AA39 (RLUC_ECOLI)

Names and origin

Protein names

Recommended name:
    Ribosomal large subunit pseudouridine synthase C
    EC=5.4.99.-
Alternative name(s):
    rRNA-uridine isomerase C
    rRNA pseudouridylate synthase C

Gene names

Name:	rluC
Synonyms:	yceC
Ordered Locus Names:	b1086, JW1072

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil at positions 955, 2504 and 2580 in 23S ribosomal RNA.
Catalytic activity	rRNA uridine = rRNA pseudouridine.
Sequence similarities	Belongs to the pseudouridine synthase rluA family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	RNA-binding
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 319

319

Ribosomal large subunit pseudouridine synthase C

PRO_0000162667

Regions

Domain

20 – 83

64

S4 RNA-binding

Sites

Active site

144

1

By similarity

Secondary structure

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319

Helix Strand Turn

Details...

Helix

91 – 94

4

Beta strand

95 – 98

4

Beta strand

100 – 107

8

Beta strand

117 – 120

4

Helix

123 – 130

8

Beta strand

138 – 141

4

Beta strand

148 – 156

9

Helix

157 – 168

12

Beta strand

172 – 182

11

Beta strand

195 – 199

5

Beta strand

205 – 209

5

Beta strand

218 – 225

8

Beta strand

227 – 238

12

Helix

243 – 250

8

Turn

259 – 261

3

Helix

264 – 272

9

Beta strand

282 – 290

9

Turn

292 – 294

3

Beta strand

297 – 301

5

Helix

306 – 317

12

Sequences

	Sequence		Length	Mass (Da)
	P0AA39-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 19C8D05E426F0344	FASTA	319	36,027
	10 20 30 40 50 60 MKTETPSVKI VAITADEAGQ RIDNFLRTQL KGVPKSMIYR ILRKGEVRVN KKRIKPEYKL 70 80 90 100 110 120 EAGDEVRIPP VRVAEREEEA VSPHLQKVAA LADVILYEDD HILVLNKPSG TAVHGGSGLS 130 140 150 160 170 180 FGVIEGLRAL RPEARFLELV HRLDRDTSGV LLVAKKRSAL RSLHEQLREK GMQKDYLALV 190 200 210 220 230 240 RGQWQSHVKS VQAPLLKNIL QSGERIVRVS QEGKPSETRF KVEERYAFAT LVRCSPVTGR 250 260 270 280 290 300 THQIRVHTQY AGHPIAFDDR YGDREFDRQL TEAGTGLNRL FLHAAALKFT HPGTGEVMRI 310 EAPMDEGLKR CLQKLRNAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Analysis of the altered mRNA stability (ams) gene from Escherichia coli. Nucleotide sequence, transcriptional analysis, and homology of its product to MRP3, a mitochondrial ribosomal protein from Neurospora crassa." Claverie-Martin F., Diaz-Torres M., Yancey S.D., Kushner S.R. J. Biol. Chem. 266:2843-2851(1991) [PubMed: 1704367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-285. Strain: K12.
[5]	"Physical locations of genes in the rne (ams)-rpmF-plsX-fab region of the Escherichia coli K-12 chromosome." Oh W., Larson T.J. J. Bacteriol. 174:7873-7874(1992) [PubMed: 1447160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 260-319. Strain: K12.
[6]	"The rluC gene of Escherichia coli codes for a pseudouridine synthase that is solely responsible for synthesis of pseudouridine at positions 955, 2504, and 2580 in 23 S ribosomal RNA." Conrad J., Sun D., Englund N., Ofengand J. J. Biol. Chem. 273:18562-18566(1998) [PubMed: 9660827] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Crystallization and characterization of a fragment of pseudouridine synthase RluC from Escherichia coli." Corollo D., Blair-Johnson M., Conrad J., Fiedler T., Sun D., Wang L., Ofengand J., Fenna R. Acta Crystallogr. D 55:302-304(1999) [PubMed: 10089432] [Abstract] Cited for: CRYSTALLIZATION OF 89-319, PROTEIN SEQUENCE OF 89-98. Strain: BL21-DE3.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U00096 Genomic DNA. Translation: AAC74170.1.
AP009048 Genomic DNA. Translation: BAA35894.1.
M62747 Genomic DNA. Translation: AAA23442.1.
M96791 Genomic DNA. Translation: AAA23828.1.

PIR

C64852.

RefSeq

AP_001712.1.
NP_415604.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V9K	X-ray	2.00	A/B	92-319	[»]
1XPI	X-ray	2.20	A/B	89-319	[»]

ModBase

Search...

Genome annotation databases

GeneID

945637.

GenomeReviews

Gene locus JW1072 in contig AP009048_GR.
Gene locus b1086 in contig U00096_GR.

KEGG

ecj:JW1072.
eco:b1086.

Organism-specific databases

EchoBASE

EB1108.

EcoGene

EG11118. rluC.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AA39.

OMA

P0AA39. QASPITG.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11118-MON.

Family and domain databases

InterPro

IPR006145. Pseudouridine_synth.
IPR006224. Pseudouridine_synth_CS.
IPR006225. Pseudouridine_synth_RluD.
IPR002942. S4_RNA_bd.
[Graphical view]

Pfam

PF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]

ProDom

PD001819. PseudoU_synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00363. S4. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00005. rluA_subfam. 1 hit.

PROSITE

PS01129. PSI_RLU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RLUC_ECOLI

Accession

Primary (citable) accession number: P0AA39
Secondary accession number(s): P23851

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)