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Protein

Ribosomal large subunit pseudouridine synthase C

Gene

rluC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil at positions 955, 2504 and 2580 in 23S ribosomal RNA.

Catalytic activityi

23S rRNA uridine(955)/uridine(2504)/uridine(2580) = 23S rRNA pseudouridine(955)/pseudouridine(2504)/pseudouridine(2580).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei144By similarity1

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoliWiki

Keywordsi

Molecular functionIsomerase, RNA-binding
Biological processrRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG11118-MONOMER
MetaCyc:EG11118-MONOMER
BRENDAi5.4.99.24 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal large subunit pseudouridine synthase C (EC:5.4.99.24)
Alternative name(s):
23S rRNA pseudouridine(955/2504/2580) synthase
rRNA pseudouridylate synthase C
rRNA-uridine isomerase C
Gene namesi
Name:rluC
Synonyms:yceC
Ordered Locus Names:b1086, JW1072
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11118 rluC

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001626671 – 319Ribosomal large subunit pseudouridine synthase CAdd BLAST319

Proteomic databases

PaxDbiP0AA39
PRIDEiP0AA39

Interactioni

Protein-protein interaction databases

BioGridi4261025, 63 interactors
850009, 1 interactor
DIPiDIP-47832N
IntActiP0AA39, 70 interactors
STRINGi316385.ECDH10B_1157

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi91 – 94Combined sources4
Beta strandi95 – 98Combined sources4
Beta strandi100 – 107Combined sources8
Beta strandi117 – 120Combined sources4
Helixi123 – 130Combined sources8
Beta strandi138 – 141Combined sources4
Beta strandi148 – 156Combined sources9
Helixi157 – 168Combined sources12
Beta strandi172 – 182Combined sources11
Beta strandi195 – 199Combined sources5
Beta strandi205 – 209Combined sources5
Beta strandi218 – 225Combined sources8
Beta strandi227 – 238Combined sources12
Helixi243 – 250Combined sources8
Turni259 – 261Combined sources3
Helixi264 – 272Combined sources9
Beta strandi282 – 290Combined sources9
Turni292 – 294Combined sources3
Beta strandi297 – 301Combined sources5
Helixi306 – 317Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V9KX-ray2.00A/B92-319[»]
1XPIX-ray2.20A/B89-319[»]
ProteinModelPortaliP0AA39
SMRiP0AA39
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AA39

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 83S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the pseudouridine synthase RluA family.Curated

Phylogenomic databases

eggNOGiENOG4105EI2 Bacteria
COG0564 LUCA
HOGENOMiHOG000275914
InParanoidiP0AA39
KOiK06179
OMAiPKTHVYR
PhylomeDBiP0AA39

Family and domain databases

CDDicd02869 PseudoU_synth_RluCD_like, 1 hit
cd00165 S4, 1 hit
Gene3Di3.10.290.10, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR006225 PsdUridine_synth_RluC/D
IPR006224 PsdUridine_synth_RluC/D_CS
IPR006145 PsdUridine_synth_RsuA/RluD
IPR002942 S4_RNA-bd
IPR036986 S4_RNA-bd_sf
PfamiView protein in Pfam
PF00849 PseudoU_synth_2, 1 hit
PF01479 S4, 1 hit
SMARTiView protein in SMART
SM00363 S4, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
TIGRFAMsiTIGR00005 rluA_subfam, 1 hit
PROSITEiView protein in PROSITE
PS01129 PSI_RLU, 1 hit
PS50889 S4, 1 hit

Sequencei

Sequence statusi: Complete.

P0AA39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTETPSVKI VAITADEAGQ RIDNFLRTQL KGVPKSMIYR ILRKGEVRVN
60 70 80 90 100
KKRIKPEYKL EAGDEVRIPP VRVAEREEEA VSPHLQKVAA LADVILYEDD
110 120 130 140 150
HILVLNKPSG TAVHGGSGLS FGVIEGLRAL RPEARFLELV HRLDRDTSGV
160 170 180 190 200
LLVAKKRSAL RSLHEQLREK GMQKDYLALV RGQWQSHVKS VQAPLLKNIL
210 220 230 240 250
QSGERIVRVS QEGKPSETRF KVEERYAFAT LVRCSPVTGR THQIRVHTQY
260 270 280 290 300
AGHPIAFDDR YGDREFDRQL TEAGTGLNRL FLHAAALKFT HPGTGEVMRI
310
EAPMDEGLKR CLQKLRNAR
Length:319
Mass (Da):36,027
Last modified:September 13, 2005 - v1
Checksum:i19C8D05E426F0344
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74170.1
AP009048 Genomic DNA Translation: BAA35894.1
M62747 Genomic DNA Translation: AAA23442.1
M96791 Genomic DNA Translation: AAA23828.1
PIRiC64852
RefSeqiNP_415604.1, NC_000913.3
WP_000846343.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74170; AAC74170; b1086
BAA35894; BAA35894; BAA35894
GeneIDi945637
KEGGiecj:JW1072
eco:b1086
PATRICifig|1411691.4.peg.1182

Similar proteinsi

Entry informationi

Entry nameiRLUC_ECOLI
AccessioniPrimary (citable) accession number: P0AA39
Secondary accession number(s): P23851
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health