P0AA38 (RLUA_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal large subunit pseudouridine synthase A
EC=5.4.99.28
EC=5.4.99.29

Alternative name(s):
23S rRNA pseudouridine(746) synthase
rRNA pseudouridylate synthase A
rRNA-uridine isomerase A
tRNA pseudouridine(32) synthase

Gene names

Name:	rluA
Ordered Locus Names:	SF0053, S0055

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs By similarity.
Catalytic activity	tRNA uridine32 = tRNA pseudouridine32. 23S rRNA uridine(746) = 23S rRNA pseudouridine(746).
Sequence similarities	Belongs to the pseudouridine synthase RluA family.
Sequence caution	The sequence AAN41719.1 differs from that shown. Reason: Frameshift at position 189.

Ontologies

Keywords
Biological process	rRNA processing tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 219	218	Ribosomal large subunit pseudouridine synthase A		PRO_0000162659
Sites
	Active site	64	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AA38 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2ADCE6D779D8C4AB	FASTA	219	24,861
	10 20 30 40 50 60 MGMENYNPPQ EPWLVILYQD DHIMVVNKPS GLLSVPGRLE EHKDSVMTRI QRDYPQAESV 70 80 90 100 110 120 HRLDMATSGV IVVALTKAAE RELKRQFRER EPKKQYVARV WGHPSPAEGL VDLPLICDWP 130 140 150 160 170 180 NRPKQKVCYE TGKPAQTEYE VVEYAADNTA RVVLKPITGR SHQLRVHMLA LGHPILGDRF 190 200 210 YASPEARAMA PRLLLHAEML TITHPAYGNS MTFKAPADF

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN41719.1. Frameshift. AE014073 Genomic DNA. Translation: AAP15599.1.
RefSeq	NP_706012.1. NC_004337.2. NP_835794.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0AA38.
SMR	P0AA38. Positions 3-219.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF0053.
Proteomic databases
PRIDE	P0AA38.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN41719; AAN41719; SF0053. AAP15599; AAP15599; S0055.
GeneID	1024562. 1076488.
KEGG	sfl:SF0053. sfx:S0055.
PATRIC	18701040. VBIShiFle31049_0062.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0564.
HOGENOM	HOG000275919.
KO	K06177.
ProtClustDB	PRK10158.
Family and domain databases
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR006225. PsdUridine_synth_RluC/D. IPR006224. PsdUridine_synth_RluC/D_CS. IPR006145. PsdUridine_synth_RsuA/RluD. [Graphical view]
Pfam	PF00849. PseudoU_synth_2. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00005. rluA_subfam. 1 hit.
PROSITE	PS01129. PSI_RLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLUA_SHIFL

Accession

Primary (citable) accession number: P0AA38
Secondary accession number(s): P39219, Q83MG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program