Ribosomal large subunit pseudouridine synthase A

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P0AA37 (RLUA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 56. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ribosomal large subunit pseudouridine synthase A
EC=5.4.99.28
EC=5.4.99.29

Alternative name(s):
23S rRNA pseudouridine(746) synthase
rRNA pseudouridylate synthase A
rRNA-uridine isomerase A
tRNA pseudouridine(32) synthase

Gene names

Name:	rluA
Synonyms:	yabO
Ordered Locus Names:	b0058, JW0057

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs. Ref.5 Ref.7

Catalytic activity

tRNA uridine(32) = tRNA pseudouridine(32). Ref.5

23S rRNA uridine(746) = 23S rRNA pseudouridine(746). Ref.5

Sequence similarities

Belongs to the pseudouridine synthase rluA family.

Ontologies

Keywords
Biological process	rRNA processing tRNA processing
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	enzyme-directed rRNA pseudouridine synthesis Inferred from mutant phenotype Ref.7. Source: EcoCyc tRNA pseudouridine synthesis Inferred from mutant phenotype Ref.7. Source: EcoCyc
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from direct assay Ref.5. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 219

218

Ribosomal large subunit pseudouridine synthase A

PRO_0000162651

Sites

Active site

Experimental info

Mutagenesis

K → M or R: Forms inclusion bodies, indicating precipitation even within the cell. Ref.8

Mutagenesis

P → G or L: Reduced structural stability and decrease in activity. Ref.8

Mutagenesis

D → N or T: Loss of activity. Ref.7

Mutagenesis

117

C → A: No change in activity. Ref.6

Mutagenesis

128

C → A: No change in activity. Ref.6

Secondary structure

219

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0AA37 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 2ADCE6D779D8C4AB
Show »

FASTA

219

24,861

        10         20         30         40         50         60 
MGMENYNPPQ EPWLVILYQD DHIMVVNKPS GLLSVPGRLE EHKDSVMTRI QRDYPQAESV 

        70         80         90        100        110        120 
HRLDMATSGV IVVALTKAAE RELKRQFRER EPKKQYVARV WGHPSPAEGL VDLPLICDWP 

       130        140        150        160        170        180 
NRPKQKVCYE TGKPAQTEYE VVEYAADNTA RVVLKPITGR SHQLRVHMLA LGHPILGDRF 

       190        200        210 
YASPEARAMA PRLLLHAEML TITHPAYGNS MTFKAPADF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"A frequently amplified region in Leishmania contains a gene conserved in prokaryotes and eukaryotes." Myler P.J., Venkataraman G.M., Lodes M.J., Stuart K.D. Gene 148:187-193(1994) [PubMed: 7958944] [Abstract] Cited for: IDENTIFICATION.
[5]	"A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe)." Wrzesinski J., Nurse K., Bakin A., Lane B.G., Ofengand J. RNA 1:437-448(1995) [PubMed: 7493321] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, PARTIAL PROTEIN SEQUENCE.
[6]	"Role of cysteine residues in pseudouridine synthases of different families." Ramamurthy V., Swann S.L., Spedaliere C.J., Mueller E.G. Biochemistry 38:13106-13111(1999) [PubMed: 10529181] [Abstract] Cited for: MUTAGENESIS OF CYS-117 AND CYS-128. Strain: BLR-DE3 and K12 / JM109 / ATCC 53323.
[7]	"Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA." Raychaudhuri S., Niu L., Conrad J., Lane B.G., Ofengand J. J. Biol. Chem. 274:18880-18886(1999) [PubMed: 10383384] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-64. Strain: BL21-DE3 and K12 / MG1655 / ATCC 47076.
[8]	"Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues." Spedaliere C.J., Hamilton C.S., Mueller E.G. Biochemistry 39:9459-9465(2000) [PubMed: 10924141] [Abstract] Cited for: MUTAGENESIS OF LYS-28 AND PRO-29. Strain: BLR-DE3.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC73169.1.
AP009048 Genomic DNA. Translation: BAE76039.1.

PIR

B64727.

RefSeq

NP_414600.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2I82	X-ray	2.05	A/B/C/D	3-218	[»]

ProteinModelPortal

P0AA37.

SMR

P0AA37. Positions 3-219.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47988N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004777; EBESCP00000004777; EBESCG00000003897.
EBESCT00000017147; EBESCP00000016438; EBESCG00000016206.

GeneID

946262.

GenomeReviews

Gene locus JW0057 in contig AP009048_GR.
Gene locus b0058 in contig U00096_GR.

KEGG

ecj:JW0057.
eco:b0058.

PATRIC

32115215. VBIEscCol129921_0059.

Organism-specific databases

EchoBASE

EB2493.

EcoGene

EG12609. rluA.

Phylogenomic databases

eggNOG

COG0564.

GeneTree

EBGT00050000009216.

HOGENOM

HBG750656.

OMA

NHILVIN.

PhylomeDB

P0AA37.

ProtClustDB

PRK10158.

Enzyme and pathway databases

BioCyc

EcoCyc:EG12609-MONOMER.
MetaCyc:EG12609-MONOMER.

Gene expression databases

Genevestigator

P0AA37.

Family and domain databases

InterPro

IPR020103. PsdUridine_synth_cat_dom.
IPR006225. PsdUridine_synth_RluC/D.
IPR006224. PsdUridine_synth_RluC/D_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
[Graphical view]

K06177.

Pfam

PF00849. PseudoU_synth_2. 1 hit.
[Graphical view]

SUPFAM

SSF55120. PsdUridine_synth_cat_dom. 1 hit.

TIGRFAMs

TIGR00005. RluA_subfam. 1 hit.

PROSITE

PS01129. PSI_RLU. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RLUA_ECOLI

Accession

Primary (citable) accession number: P0AA37
Secondary accession number(s): P39219, Q2MCG7, Q83MG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents