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Protein

Ribosomal large subunit pseudouridine synthase A

Gene

rluA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs.2 Publications

Catalytic activityi

tRNA uridine(32) = tRNA pseudouridine(32).1 Publication
23S rRNA uridine(746) = 23S rRNA pseudouridine(746).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: InterPro

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
  • pseudouridine synthesis Source: EcoliWiki
  • RNA modification Source: EcoliWiki
  • rRNA processing Source: EcoliWiki
  • rRNA pseudouridine synthesis Source: EcoliWiki
  • tRNA processing Source: EcoliWiki
  • tRNA pseudouridine synthesis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

rRNA processing, tRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG12609-MONOMER.
ECOL316407:JW0057-MONOMER.
MetaCyc:EG12609-MONOMER.
BRENDAi5.4.99.28. 2026.
5.4.99.29. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal large subunit pseudouridine synthase A (EC:5.4.99.28, EC:5.4.99.29)
Alternative name(s):
23S rRNA pseudouridine(746) synthase
rRNA pseudouridylate synthase A
rRNA-uridine isomerase A
tRNA pseudouridine(32) synthase
Gene namesi
Name:rluA
Synonyms:yabO
Ordered Locus Names:b0058, JW0057
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12609. rluA.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi28 – 281K → M or R: Forms inclusion bodies, indicating precipitation even within the cell. 1 Publication
Mutagenesisi29 – 291P → G or L: Reduced structural stability and decrease in activity. 1 Publication
Mutagenesisi64 – 641D → N or T: Loss of activity. 1 Publication
Mutagenesisi117 – 1171C → A: No change in activity. 1 Publication
Mutagenesisi128 – 1281C → A: No change in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 219218Ribosomal large subunit pseudouridine synthase APRO_0000162651Add
BLAST

Proteomic databases

PaxDbiP0AA37.
PRIDEiP0AA37.

Interactioni

Protein-protein interaction databases

BioGridi4262040. 11 interactions.
DIPiDIP-47988N.
IntActiP0AA37. 4 interactions.
STRINGi511145.b0058.

Structurei

Secondary structure

1
219
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 133Combined sources
Beta strandi16 – 194Combined sources
Beta strandi21 – 288Combined sources
Beta strandi32 – 354Combined sources
Helixi40 – 423Combined sources
Helixi46 – 538Combined sources
Beta strandi58 – 614Combined sources
Beta strandi68 – 769Combined sources
Helixi77 – 8812Combined sources
Beta strandi92 – 10211Combined sources
Beta strandi105 – 1128Combined sources
Beta strandi115 – 1173Combined sources
Helixi119 – 1213Combined sources
Beta strandi125 – 1273Combined sources
Turni129 – 1313Combined sources
Beta strandi136 – 14510Combined sources
Turni146 – 1483Combined sources
Beta strandi149 – 15810Combined sources
Helixi163 – 1708Combined sources
Turni179 – 1813Combined sources
Helixi184 – 1885Combined sources
Beta strandi195 – 2039Combined sources
Turni205 – 2073Combined sources
Beta strandi210 – 2145Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I82X-ray2.05A/B/C/D3-219[»]
ProteinModelPortaliP0AA37.
SMRiP0AA37. Positions 3-219.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AA37.

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase RluA family.Curated

Phylogenomic databases

eggNOGiENOG4105D3G. Bacteria.
COG0564. LUCA.
HOGENOMiHOG000275919.
InParanoidiP0AA37.
KOiK06177.
OMAiEKYGFCE.
PhylomeDBiP0AA37.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR006225. PsdUridine_synth_RluC/D.
IPR006224. PsdUridine_synth_RluC/D_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00005. rluA_subfam. 1 hit.
PROSITEiPS01129. PSI_RLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AA37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMENYNPPQ EPWLVILYQD DHIMVVNKPS GLLSVPGRLE EHKDSVMTRI
60 70 80 90 100
QRDYPQAESV HRLDMATSGV IVVALTKAAE RELKRQFRER EPKKQYVARV
110 120 130 140 150
WGHPSPAEGL VDLPLICDWP NRPKQKVCYE TGKPAQTEYE VVEYAADNTA
160 170 180 190 200
RVVLKPITGR SHQLRVHMLA LGHPILGDRF YASPEARAMA PRLLLHAEML
210
TITHPAYGNS MTFKAPADF
Length:219
Mass (Da):24,861
Last modified:January 23, 2007 - v2
Checksum:i2ADCE6D779D8C4AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73169.1.
AP009048 Genomic DNA. Translation: BAE76039.1.
PIRiB64727.
RefSeqiNP_414600.1. NC_000913.3.
WP_000525176.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73169; AAC73169; b0058.
BAE76039; BAE76039; BAE76039.
GeneIDi946262.
KEGGiecj:JW0057.
eco:b0058.
PATRICi32115215. VBIEscCol129921_0059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73169.1.
AP009048 Genomic DNA. Translation: BAE76039.1.
PIRiB64727.
RefSeqiNP_414600.1. NC_000913.3.
WP_000525176.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I82X-ray2.05A/B/C/D3-219[»]
ProteinModelPortaliP0AA37.
SMRiP0AA37. Positions 3-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262040. 11 interactions.
DIPiDIP-47988N.
IntActiP0AA37. 4 interactions.
STRINGi511145.b0058.

Proteomic databases

PaxDbiP0AA37.
PRIDEiP0AA37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73169; AAC73169; b0058.
BAE76039; BAE76039; BAE76039.
GeneIDi946262.
KEGGiecj:JW0057.
eco:b0058.
PATRICi32115215. VBIEscCol129921_0059.

Organism-specific databases

EchoBASEiEB2493.
EcoGeneiEG12609. rluA.

Phylogenomic databases

eggNOGiENOG4105D3G. Bacteria.
COG0564. LUCA.
HOGENOMiHOG000275919.
InParanoidiP0AA37.
KOiK06177.
OMAiEKYGFCE.
PhylomeDBiP0AA37.

Enzyme and pathway databases

BioCyciEcoCyc:EG12609-MONOMER.
ECOL316407:JW0057-MONOMER.
MetaCyc:EG12609-MONOMER.
BRENDAi5.4.99.28. 2026.
5.4.99.29. 2026.

Miscellaneous databases

EvolutionaryTraceiP0AA37.
PROiP0AA37.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR006225. PsdUridine_synth_RluC/D.
IPR006224. PsdUridine_synth_RluC/D_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00005. rluA_subfam. 1 hit.
PROSITEiPS01129. PSI_RLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLUA_ECOLI
AccessioniPrimary (citable) accession number: P0AA37
Secondary accession number(s): P39219, Q2MCG7, Q83MG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.