Transcriptional regulatory protein OmpR - Escherichia coli (strain K12)

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P0AA16 (OMPR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 76. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein OmpR

Gene names

Name:	ompR
Synonyms:	kmt, ompB
Ordered Locus Names:	b3405, JW3368

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The N-terminus of this protein is required for the transcriptional expression of both major outer membrane protein genes ompF and ompC; its C-terminal moiety mediates the multimerization of the OmpR protein. As a multimer, it turns on the expression of the ompC gene; as a monomer, it turns on the expression of the ompF gene.
Subunit structure	Monomer and multimer.
Subcellular location	Cytoplasm.
Post-translational modification	Phosphorylated by EnvZ. Asp-55 is the primary phosphate acceptor site, but Asp-11 may also serve as a phosphorylation site, particularly in the absence of Asp-55. Ref.6
Sequence similarities	Contains 1 response regulatory domain.
Sequence caution	The sequence described in Ref.1 differs from that shown. Reason: Frameshift at position 210.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 2558046. Source: EcoliWiki transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from direct assay PubMed 2558046. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 239

239

Transcriptional regulatory protein OmpR

PRO_0000081176

Regions

Domain

1 – 120

120

Response regulatory

Amino acid modifications

Modified residue

4-aspartylphosphate

Experimental info

Sequence conflict

I → N Ref.2

Secondary structure

239

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0AA16 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 823CA7720E9A1D2A
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FASTA

239

27,354

        10         20         30         40         50         60 
MQENYKILVV DDDMRLRALL ERYLTEQGFQ VRSVANAEQM DRLLTRESFH LMVLDLMLPG 

        70         80         90        100        110        120 
EDGLSICRRL RSQSNPMPII MVTAKGEEVD RIVGLEIGAD DYIPKPFNPR ELLARIRAVL 

       130        140        150        160        170        180 
RRQANELPGA PSQEEAVIAF GKFKLNLGTR EMFREDEPMP LTSGEFAVLK ALVSHPREPL 

       190        200        210        220        230 
SRDKLMNLAR GREYSAMERS IDVQISRLRR MVEEDPAHPR YIQTVWGLGY VFVPDGSKA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Osmoregulation of gene expression. I. DNA sequence of the ompR gene of the ompB operon of Escherichia coli and characterization of its gene product." Wurtzel E.T., Chou M.-Y., Inouye M. J. Biol. Chem. 257:13685-13691(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular analysis of mutant ompR genes exhibiting different phenotypes as to osmoregulation of the ompF and ompC genes of Escherichia coli." Nara F., Matsuyama S., Mizuno T., Mizushima S. Mol. Gen. Genet. 202:194-199(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins." Comeau D.E., Ikenaka K., Tsung K., Inouye M. J. Bacteriol. 164:578-584(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Identification of a phosphorylation site and functional analysis of conserved aspartic acid residues of OmpR, a transcriptional activator for ompF and ompC in Escherichia coli." Delgado J., Forst S., Harlocker S., Inouye M. Mol. Microbiol. 10:1037-1047(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION.
[7]	"Escherichia coli positive regulator OmpR has a large loop structure at the putative RNA polymerase interaction site." Kondo H., Nakagawa A., Nishihira J., Nishimura Y., Mizuno T., Tanaka I. Nat. Struct. Biol. 4:28-31(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 136-235.
[8]	"The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factor." Martinez-Hackert E., Stock A.M. Structure 5:109-124(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 137-235.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01656 Unassigned RNA. Translation: AAA16241.1.
U18997 Genomic DNA. Translation: AAA58202.1.
U00096 Genomic DNA. Translation: AAC76430.1.
AP009048 Genomic DNA. Translation: BAE77886.1.

PIR

RGECOR. H65135.

RefSeq

NP_417864.1. NC_000913.2.
YP_492027.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ODD	X-ray	2.20	A	122-239	[»]
1OPC	X-ray	1.95	A	130-239	[»]
2JPB	NMR	-	A	136-239	[»]

ProteinModelPortal

P0AA16.

SMR

P0AA16. Positions 4-235.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-31859N.

IntAct

P0AA16. 9 interactions.

STRING

511145.b3405.

Proteomic databases

PaxDb

P0AA16.

PRIDE

P0AA16.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76430; AAC76430; b3405.
BAE77886; BAE77886; BAE77886.

GeneID

12932273.
947913.

KEGG

ecj:Y75_p3771.
eco:b3405.

PATRIC

32122246. VBIEscCol129921_3500.

Organism-specific databases

EchoBASE

EB0666.

EcoGene

EG10672. ompR.

Phylogenomic databases

eggNOG

COG0745.

HOGENOM

HOG000034819.

K07659.

OMA

REPMSRD.

ProtClustDB

PRK09468.

Enzyme and pathway databases

BioCyc

EcoCyc:OMPR-MONOMER.
ECOL316407:JW3368-MONOMER.

Gene expression databases

Genevestigator

P0AA16.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]

SMART

SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]

SUPFAM

SSF52172. CheY_like. 1 hit.

PROSITE

PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0AA16.

Entry information

Entry name

OMPR_ECOLI

Accession

Primary (citable) accession number: P0AA16
Secondary accession number(s): O31133

Q2M770

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents