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P0AA16

- OMPR_ECOLI

UniProt

P0AA16 - OMPR_ECOLI

Protein

Transcriptional regulatory protein OmpR

Gene

ompR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    The N-terminus of this protein is required for the transcriptional expression of both major outer membrane protein genes ompF and ompC; its C-terminal moiety mediates the multimerization of the OmpR protein. As a multimer, it turns on the expression of the ompC gene; as a monomer, it turns on the expression of the ompF gene.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. phosphorelay response regulator activity Source: EcoliWiki

    GO - Biological processi

    1. intracellular signal transduction Source: GOC
    2. phosphorelay signal transduction system Source: EcoliWiki
    3. positive regulation of transcription, DNA-templated Source: EcoliWiki
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:OMPR-MONOMER.
    ECOL316407:JW3368-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional regulatory protein OmpR
    Gene namesi
    Name:ompR
    Synonyms:kmt, ompB
    Ordered Locus Names:b3405, JW3368
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10672. ompR.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 239239Transcriptional regulatory protein OmpRPRO_0000081176Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei55 – 5514-aspartylphosphate1 PublicationPROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylated by EnvZ. Asp-55 is the primary phosphate acceptor site, but Asp-11 may also serve as a phosphorylation site, particularly in the absence of Asp-55.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP0AA16.
    PRIDEiP0AA16.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AA16.

    Interactioni

    Subunit structurei

    Monomer and multimer.

    Protein-protein interaction databases

    DIPiDIP-31859N.
    IntActiP0AA16. 10 interactions.
    STRINGi511145.b3405.

    Structurei

    Secondary structure

    1
    239
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi138 – 1403
    Beta strandi143 – 1464
    Turni147 – 1504
    Beta strandi151 – 1544
    Beta strandi157 – 1593
    Helixi163 – 17412
    Helixi182 – 1898
    Beta strandi192 – 1943
    Helixi201 – 21212
    Beta strandi220 – 2256
    Turni226 – 2283
    Beta strandi229 – 2324

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ODDX-ray2.20A122-239[»]
    1OPCX-ray1.95A130-239[»]
    2JPBNMR-A136-239[»]
    ProteinModelPortaliP0AA16.
    SMRiP0AA16. Positions 4-235.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AA16.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 120120Response regulatoryPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0745.
    HOGENOMiHOG000034819.
    KOiK07659.
    OMAiLRNANNM.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiP0AA16.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AA16-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQENYKILVV DDDMRLRALL ERYLTEQGFQ VRSVANAEQM DRLLTRESFH    50
    LMVLDLMLPG EDGLSICRRL RSQSNPMPII MVTAKGEEVD RIVGLEIGAD 100
    DYIPKPFNPR ELLARIRAVL RRQANELPGA PSQEEAVIAF GKFKLNLGTR 150
    EMFREDEPMP LTSGEFAVLK ALVSHPREPL SRDKLMNLAR GREYSAMERS 200
    IDVQISRLRR MVEEDPAHPR YIQTVWGLGY VFVPDGSKA 239
    Length:239
    Mass (Da):27,354
    Last modified:September 13, 2005 - v1
    Checksum:i823CA7720E9A1D2A
    GO

    Sequence cautioni

    The sequence described in 1 Publication differs from that shown. Reason: Frameshift at position 210.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71I → N(PubMed:3010044)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01656 Unassigned RNA. Translation: AAA16241.1.
    U18997 Genomic DNA. Translation: AAA58202.1.
    U00096 Genomic DNA. Translation: AAC76430.1.
    AP009048 Genomic DNA. Translation: BAE77886.1.
    PIRiH65135. RGECOR.
    RefSeqiNP_417864.1. NC_000913.3.
    YP_492027.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76430; AAC76430; b3405.
    BAE77886; BAE77886; BAE77886.
    GeneIDi12932273.
    947913.
    KEGGiecj:Y75_p3771.
    eco:b3405.
    PATRICi32122246. VBIEscCol129921_3500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01656 Unassigned RNA. Translation: AAA16241.1 .
    U18997 Genomic DNA. Translation: AAA58202.1 .
    U00096 Genomic DNA. Translation: AAC76430.1 .
    AP009048 Genomic DNA. Translation: BAE77886.1 .
    PIRi H65135. RGECOR.
    RefSeqi NP_417864.1. NC_000913.3.
    YP_492027.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ODD X-ray 2.20 A 122-239 [» ]
    1OPC X-ray 1.95 A 130-239 [» ]
    2JPB NMR - A 136-239 [» ]
    ProteinModelPortali P0AA16.
    SMRi P0AA16. Positions 4-235.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-31859N.
    IntActi P0AA16. 10 interactions.
    STRINGi 511145.b3405.

    Proteomic databases

    PaxDbi P0AA16.
    PRIDEi P0AA16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76430 ; AAC76430 ; b3405 .
    BAE77886 ; BAE77886 ; BAE77886 .
    GeneIDi 12932273.
    947913.
    KEGGi ecj:Y75_p3771.
    eco:b3405.
    PATRICi 32122246. VBIEscCol129921_3500.

    Organism-specific databases

    EchoBASEi EB0666.
    EcoGenei EG10672. ompR.

    Phylogenomic databases

    eggNOGi COG0745.
    HOGENOMi HOG000034819.
    KOi K07659.
    OMAi LRNANNM.
    OrthoDBi EOG6G4VQG.
    PhylomeDBi P0AA16.

    Enzyme and pathway databases

    BioCyci EcoCyc:OMPR-MONOMER.
    ECOL316407:JW3368-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AA16.
    PROi P0AA16.

    Gene expression databases

    Genevestigatori P0AA16.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Osmoregulation of gene expression. I. DNA sequence of the ompR gene of the ompB operon of Escherichia coli and characterization of its gene product."
      Wurtzel E.T., Chou M.-Y., Inouye M.
      J. Biol. Chem. 257:13685-13691(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Molecular analysis of mutant ompR genes exhibiting different phenotypes as to osmoregulation of the ompF and ompC genes of Escherichia coli."
      Nara F., Matsuyama S., Mizuno T., Mizushima S.
      Mol. Gen. Genet. 202:194-199(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins."
      Comeau D.E., Ikenaka K., Tsung K., Inouye M.
      J. Bacteriol. 164:578-584(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Identification of a phosphorylation site and functional analysis of conserved aspartic acid residues of OmpR, a transcriptional activator for ompF and ompC in Escherichia coli."
      Delgado J., Forst S., Harlocker S., Inouye M.
      Mol. Microbiol. 10:1037-1047(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT ASP-55.
    7. "Escherichia coli positive regulator OmpR has a large loop structure at the putative RNA polymerase interaction site."
      Kondo H., Nakagawa A., Nishihira J., Nishimura Y., Mizuno T., Tanaka I.
      Nat. Struct. Biol. 4:28-31(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 136-235.
    8. "The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factor."
      Martinez-Hackert E., Stock A.M.
      Structure 5:109-124(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 137-235.

    Entry informationi

    Entry nameiOMPR_ECOLI
    AccessioniPrimary (citable) accession number: P0AA16
    Secondary accession number(s): O31133
    , P03025, P08981, P41405, Q2M770
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3