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Protein

Transcriptional regulatory protein OmpR

Gene

ompR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The N-terminus of this protein is required for the transcriptional expression of both major outer membrane protein genes ompF and ompC; its C-terminal moiety mediates the multimerization of the OmpR protein. As a multimer, it turns on the expression of the ompC gene; as a monomer, it turns on the expression of the ompF gene.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi135 – 234OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST100

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • phosphorelay response regulator activity Source: EcoliWiki

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoliWiki
  • positive regulation of transcription, DNA-templated Source: EcoliWiki
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:OMPR-MONOMER.
ECOL316407:JW3368-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein OmpR
Gene namesi
Name:ompR
Synonyms:kmt, ompB
Ordered Locus Names:b3405, JW3368
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10672. ompR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000811761 – 239Transcriptional regulatory protein OmpRAdd BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei554-aspartylphosphatePROSITE-ProRule annotation1 Publication1

Post-translational modificationi

Phosphorylated by EnvZ. Asp-55 is the primary phosphate acceptor site, but Asp-11 may also serve as a phosphorylation site, particularly in the absence of Asp-55.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP0AA16.
PaxDbiP0AA16.
PRIDEiP0AA16.

Expressioni

Gene expression databases

CollecTFiEXPREG_00000810.

Interactioni

Subunit structurei

Monomer and multimer.

Protein-protein interaction databases

BioGridi4261267. 6 interactors.
DIPiDIP-31859N.
IntActiP0AA16. 10 interactors.
STRINGi511145.b3405.

Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi138 – 140Combined sources3
Beta strandi143 – 146Combined sources4
Turni147 – 150Combined sources4
Beta strandi151 – 154Combined sources4
Beta strandi157 – 159Combined sources3
Helixi163 – 174Combined sources12
Helixi182 – 189Combined sources8
Beta strandi192 – 194Combined sources3
Helixi201 – 212Combined sources12
Beta strandi220 – 225Combined sources6
Turni226 – 228Combined sources3
Beta strandi229 – 232Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ODDX-ray2.20A122-239[»]
1OPCX-ray1.95A130-239[»]
2JPBNMR-A136-239[»]
ProteinModelPortaliP0AA16.
SMRiP0AA16.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AA16.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 120Response regulatoryPROSITE-ProRule annotationAdd BLAST115

Sequence similaritiesi

Contains 1 OmpR/PhoB-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000034819.
InParanoidiP0AA16.
KOiK07659.
OMAiMDVERHI.
PhylomeDBiP0AA16.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AA16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQENYKILVV DDDMRLRALL ERYLTEQGFQ VRSVANAEQM DRLLTRESFH
60 70 80 90 100
LMVLDLMLPG EDGLSICRRL RSQSNPMPII MVTAKGEEVD RIVGLEIGAD
110 120 130 140 150
DYIPKPFNPR ELLARIRAVL RRQANELPGA PSQEEAVIAF GKFKLNLGTR
160 170 180 190 200
EMFREDEPMP LTSGEFAVLK ALVSHPREPL SRDKLMNLAR GREYSAMERS
210 220 230
IDVQISRLRR MVEEDPAHPR YIQTVWGLGY VFVPDGSKA
Length:239
Mass (Da):27,354
Last modified:September 13, 2005 - v1
Checksum:i823CA7720E9A1D2A
GO

Sequence cautioni

The sequence described in PubMed:6292199 differs from that shown. Reason: Frameshift at position 210.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7I → N (PubMed:3010044).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01656 Unassigned RNA. Translation: AAA16241.1.
U18997 Genomic DNA. Translation: AAA58202.1.
U00096 Genomic DNA. Translation: AAC76430.1.
AP009048 Genomic DNA. Translation: BAE77886.1.
PIRiH65135. RGECOR.
RefSeqiNP_417864.1. NC_000913.3.
WP_001157751.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76430; AAC76430; b3405.
BAE77886; BAE77886; BAE77886.
GeneIDi8912757.
947913.
KEGGiecj:JW3368.
eco:b3405.
PATRICi32122246. VBIEscCol129921_3500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01656 Unassigned RNA. Translation: AAA16241.1.
U18997 Genomic DNA. Translation: AAA58202.1.
U00096 Genomic DNA. Translation: AAC76430.1.
AP009048 Genomic DNA. Translation: BAE77886.1.
PIRiH65135. RGECOR.
RefSeqiNP_417864.1. NC_000913.3.
WP_001157751.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ODDX-ray2.20A122-239[»]
1OPCX-ray1.95A130-239[»]
2JPBNMR-A136-239[»]
ProteinModelPortaliP0AA16.
SMRiP0AA16.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261267. 6 interactors.
DIPiDIP-31859N.
IntActiP0AA16. 10 interactors.
STRINGi511145.b3405.

Proteomic databases

EPDiP0AA16.
PaxDbiP0AA16.
PRIDEiP0AA16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76430; AAC76430; b3405.
BAE77886; BAE77886; BAE77886.
GeneIDi8912757.
947913.
KEGGiecj:JW3368.
eco:b3405.
PATRICi32122246. VBIEscCol129921_3500.

Organism-specific databases

EchoBASEiEB0666.
EcoGeneiEG10672. ompR.

Phylogenomic databases

HOGENOMiHOG000034819.
InParanoidiP0AA16.
KOiK07659.
OMAiMDVERHI.
PhylomeDBiP0AA16.

Enzyme and pathway databases

BioCyciEcoCyc:OMPR-MONOMER.
ECOL316407:JW3368-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AA16.
PROiP0AA16.

Gene expression databases

CollecTFiEXPREG_00000810.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOMPR_ECOLI
AccessioniPrimary (citable) accession number: P0AA16
Secondary accession number(s): O31133
, P03025, P08981, P41405, Q2M770
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.