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P0AA10 (RL13_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L13
Gene names
Name:rplM
Ordered Locus Names:b3231, JW3200
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length142 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. HAMAP-Rule MF_01366

Subunit structure

Part of the 50S ribosomal subunit. Ref.6

Sequence similarities

Belongs to the ribosomal protein L13P family.

Mass spectrometry

Molecular mass is 16018.0 Da from positions 1 - 142. Determined by MALDI. Ref.7

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14214250S ribosomal protein L13 HAMAP-Rule MF_01366
PRO_0000133734

Secondary structure

1 ...................................... 142
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AA10 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 58B45EF9EB75FDCD

FASTA14216,019
        10         20         30         40         50         60 
MKTFTAKPET VKRDWYVVDA TGKTLGRLAT ELARRLRGKH KAEYTPHVDT GDYIIVLNAD 

        70         80         90        100        110        120 
KVAVTGNKRT DKVYYHHTGH IGGIKQATFE EMIARRPERV IEIAVKGMLP KGPLGRAMFR 

       130        140 
KLKVYAGNEH NHAAQQPQVL DI

« Hide

References

« Hide 'large scale' references
[1]"The primary structure of protein L13 from the large subunit of Escherichia coli ribosomes."
Mende L.
FEBS Lett. 96:313-316(1978) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Strain: K.
[2]"Cloning and nucleotide sequencing of the genes for ribosomal proteins S9 (rpsI) and L13 (rplM) of Escherichia coli."
Isono S., Thamm S., Kitakawa M., Isono K.
Mol. Gen. Genet. 198:279-282(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 34-46.
Strain: K12 / EMG2.
[6]"Incorporation of six additional proteins to complete the assembly map of the 50 S subunit from Escherichia coli ribosomes."
Herold M., Nierhaus K.H.
J. Biol. Chem. 262:8826-8833(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSEMBLY MAP OF THE 50S SUBUNIT.
Strain: K12.
[7]"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry."
Arnold R.J., Reilly J.P.
Anal. Biochem. 269:105-112(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[8]"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement."
Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.
Cell 113:789-801(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS).
Strain: MRE-600.
[9]"Structures of the bacterial ribosome at 3.5 A resolution."
Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.
Science 310:827-834(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES.
Strain: MRE-600.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X02130 Genomic DNA. Translation: CAA26041.1.
U18997 Genomic DNA. Translation: AAA58033.1.
U00096 Genomic DNA. Translation: AAC76263.1.
AP009048 Genomic DNA. Translation: BAE77274.1.
PIRR5EC13. A02787.
RefSeqNP_417698.1. NC_000913.2.
YP_491415.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ML5electron microscopy14.00m1-142[»]
1P85electron microscopy12.30H1-142[»]
1P86electron microscopy11.50H1-142[»]
1VS6X-ray3.46J1-142[»]
1VS8X-ray3.46J1-142[»]
1VT2X-ray3.30J1-142[»]
2AW4X-ray3.46J1-142[»]
2AWBX-ray3.46J1-142[»]
2GYAelectron microscopy15.00H1-142[»]
2GYCelectron microscopy15.00H1-142[»]
2I2TX-ray3.22J1-142[»]
2I2VX-ray3.22J1-142[»]
2J28electron microscopy8.00J1-140[»]
2QAMX-ray3.21J1-142[»]
2QAOX-ray3.21J1-142[»]
2QBAX-ray3.54J1-142[»]
2QBCX-ray3.54J1-142[»]
2QBEX-ray3.30J1-142[»]
2QBGX-ray3.30J1-142[»]
2QBIX-ray4.00J1-142[»]
2QBKX-ray4.00J1-142[»]
2QOVX-ray3.93J1-142[»]
2QOXX-ray3.93J1-142[»]
2QOZX-ray3.50J1-142[»]
2QP1X-ray3.50J1-142[»]
2RDOelectron microscopy9.10J1-142[»]
2WWQelectron microscopy5.80J1-142[»]
2Z4LX-ray4.45J1-142[»]
2Z4NX-ray4.45J1-142[»]
3BBXelectron microscopy10.00J1-142[»]
3E1Belectron microscopy-61-140[»]
3E1Delectron microscopy-61-140[»]
3FIKelectron microscopy6.70J1-142[»]
3I1NX-ray3.19J1-142[»]
3I1PX-ray3.19J1-142[»]
3I1RX-ray3.81J1-142[»]
3I1TX-ray3.81J1-142[»]
3I20X-ray3.71J1-142[»]
3I22X-ray3.71J1-142[»]
3IZTelectron microscopy-K1-142[»]
3IZUelectron microscopy-K1-142[»]
3J01electron microscopy-J1-142[»]
3J0Telectron microscopy12.10L1-142[»]
3J0Welectron microscopy14.70L1-142[»]
3J0Yelectron microscopy13.50L1-142[»]
3J11electron microscopy13.10L1-142[»]
3J12electron microscopy11.50L1-142[»]
3J14electron microscopy11.50L1-142[»]
3J19electron microscopy8.30J1-142[»]
3KCRelectron microscopy-J1-142[»]
3OASX-ray3.25J1-142[»]
3OATX-ray3.25J1-142[»]
3OFCX-ray3.19J1-142[»]
3OFDX-ray3.19J1-142[»]
3OFQX-ray3.10J1-142[»]
3OFRX-ray3.10J1-142[»]
3OFZX-ray3.29J1-142[»]
3OG0X-ray3.29J1-142[»]
3ORBX-ray3.30J1-142[»]
3R8SX-ray3.00J1-142[»]
3R8TX-ray3.00J1-142[»]
3SGFX-ray3.20N1-142[»]
3UOSX-ray3.70N1-142[»]
4GARX-ray3.30J1-142[»]
4GAUX-ray3.30J1-142[»]
ProteinModelPortalP0AA10.
SMRP0AA10. Positions 1-142.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-47837N.
IntActP0AA10. 111 interactions.
MINTMINT-1269598.
STRING511145.b3231.

Proteomic databases

PaxDbP0AA10.
PRIDEP0AA10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76263; AAC76263; b3231.
BAE77274; BAE77274; BAE77274.
GeneID12932920.
947828.
KEGGecj:Y75_p3151.
eco:b3231.
PATRIC32121888. VBIEscCol129921_3328.

Organism-specific databases

EchoBASEEB0867.
EcoGeneEG10874. rplM.

Phylogenomic databases

eggNOGCOG0102.
HOGENOMHOG000225286.
KOK02871.
OMAYTPHMDC.
ProtClustDBPRK09216.

Enzyme and pathway databases

BioCycEcoCyc:EG10874-MONOMER.
ECOL316407:JW3200-MONOMER.

Gene expression databases

GenevestigatorP0AA10.

Family and domain databases

Gene3D3.90.1180.10. 1 hit.
HAMAPMF_01366_B. Ribosomal_L13_B.
InterProIPR005822. Ribosomal_L13.
IPR005823. Ribosomal_L13_bac-type.
IPR023563. Ribosomal_L13_CS.
IPR023564. Ribosomal_L13_dom.
[Graphical view]
PANTHERPTHR11545. PTHR11545. 1 hit.
PfamPF00572. Ribosomal_L13. 1 hit.
[Graphical view]
PIRSFPIRSF002181. Ribosomal_L13. 1 hit.
SUPFAMSSF52161. Ribosomal_L13. 1 hit.
TIGRFAMsTIGR01066. rplM_bact. 1 hit.
PROSITEPS00783. RIBOSOMAL_L13. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0AA10.

Entry information

Entry nameRL13_ECOLI
AccessionPrimary (citable) accession number: P0AA10
Secondary accession number(s): P02410, Q2M8Y2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Ribosomal proteins

Ribosomal proteins families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents