Reviewed, UniProtKB/Swiss-Prot P0AA10 (RL13_ECOLI)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L13

Gene names

Name:	rplM
Ordered Locus Names:	b3231, JW3200

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. HAMAP MF_01366
Subunit structure	Part of the 50S ribosomal subunit. Ref.6
Sequence similarities	Belongs to the ribosomal protein L13P family.
Mass spectrometry	Molecular mass is 16018.0 Da from positions 1 - 142. Determined by MALDI. Ref.7

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 142

142

50S ribosomal protein L13 HAMAP MF_01366

PRO_0000133734

Secondary structure

1

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142

Helix Strand Turn

Details...

Helix

25 – 27

3

Helix

28 – 33

6

Turn

34 – 37

4

Beta strand

38 – 40

3

Beta strand

42 – 44

3

Beta strand

46 – 49

4

Helix

67 – 69

3

Helix

90 – 93

4

Helix

101 – 108

8

Turn

113 – 122

10

Beta strand

126 – 130

5

Sequences

	Sequence		Length	Mass (Da)
	P0AA10-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 58B45EF9EB75FDCD	FASTA	142	16,019
	10 20 30 40 50 60 MKTFTAKPET VKRDWYVVDA TGKTLGRLAT ELARRLRGKH KAEYTPHVDT GDYIIVLNAD 70 80 90 100 110 120 KVAVTGNKRT DKVYYHHTGH IGGIKQATFE EMIARRPERV IEIAVKGMLP KGPLGRAMFR 130 140 KLKVYAGNEH NHAAQQPQVL DI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of protein L13 from the large subunit of Escherichia coli ribosomes." Mende L. FEBS Lett. 96:313-316(1978) [PubMed: 365580] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: K.
[2]	"Cloning and nucleotide sequencing of the genes for ribosomal proteins S9 (rpsI) and L13 (rplM) of Escherichia coli." Isono S., Thamm S., Kitakawa M., Isono K. Mol. Gen. Genet. 198:279-282(1985) [PubMed: 3884974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 34-46. Strain: K12 / EMG2.
[6]	"Incorporation of six additional proteins to complete the assembly map of the 50 S subunit from Escherichia coli ribosomes." Herold M., Nierhaus K.H. J. Biol. Chem. 262:8826-8833(1987) [PubMed: 3298242] [Abstract] Cited for: ASSEMBLY MAP OF THE 50S SUBUNIT. Strain: K12.
[7]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[8]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[9]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X02130 Genomic DNA. Translation: CAA26041.1.
U18997 Genomic DNA. Translation: AAA58033.1.
U00096 Genomic DNA. Translation: AAC76263.1.
AP009048 Genomic DNA. Translation: BAE77274.1.

PIR

R5EC13. A02787.

RefSeq

AP_003773.1.
NP_417698.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P85	electron microscopy	12.30	H	1-142	[»]
1P86	electron microscopy	11.50	H	1-142	[»]
1VS6	X-ray	3.46	J	1-142	[»]
1VS8	X-ray	3.46	J	1-142	[»]
2AW4	X-ray	3.46	J	1-142	[»]
2AWB	X-ray	3.46	J	1-142	[»]
2GYA	electron microscopy	15.00	H	1-142	[»]
2GYC	electron microscopy	15.00	H	1-142	[»]
2I2T	X-ray	3.22	J	1-142	[»]
2I2V	X-ray	3.22	J	1-142	[»]
2J28	electron microscopy	8.00	J	1-140	[»]
2QAM	X-ray	3.21	J	1-142	[»]
2QAO	X-ray	3.21	J	1-142	[»]
2QBA	X-ray	3.54	J	1-142	[»]
2QBC	X-ray	3.54	J	1-142	[»]
2QBE	X-ray	3.30	J	1-142	[»]
2QBG	X-ray	3.30	J	1-142	[»]
2QBI	X-ray	4.00	J	1-142	[»]
2QBK	X-ray	4.00	J	1-142	[»]
2QOV	X-ray	3.93	J	1-142	[»]
2QOX	X-ray	3.93	J	1-142	[»]
2QOZ	X-ray	3.50	J	1-142	[»]
2QP1	X-ray	3.50	J	1-142	[»]
2RDO	electron microscopy	9.10	J	1-142	[»]
2Z4L	X-ray	4.45	J	1-142	[»]
2Z4N	X-ray	4.45	J	1-142	[»]
3BBX	electron microscopy	10.00	J	1-142	[»]
3E1B	electron microscopy	-	6	1-140	[»]
3E1D	electron microscopy	-	6	1-140	[»]
3FIK	electron microscopy	6.70	J	1-142	[»]
3I1N	X-ray	3.19	J	1-142	[»]
3I1P	X-ray	3.19	J	1-142	[»]
3I1R	X-ray	3.81	J	1-142	[»]
3I1T	X-ray	3.81	J	1-142	[»]
3I20	X-ray	3.71	J	1-142	[»]
3I22	X-ray	3.71	J	1-142	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0AA10.

Proteomic databases

PRIDE

P0AA10.

Genome annotation databases

GeneID

947828.

GenomeReviews

Gene locus JW3200 in contig AP009048_GR.
Gene locus b3231 in contig U00096_GR.

KEGG

ecj:JW3200.
eco:b3231.

Organism-specific databases

EchoBASE

EB0867.

EcoGene

EG10874. rplM.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AA10.

OMA

YTPHMDC.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10874-MON.

Gene expression databases

Genevestigator

P0AA10.

Family and domain databases

HAMAP

MF_01366.
[Tree]

InterPro

IPR005822. Ribosomal_L13.
IPR005823. Ribosomal_L13_bac-type.
[Graphical view]

Gene3D

G3DSA:3.90.1180.10. Ribosomal_L13. 1 hit.

PANTHER

PTHR11545:SF2. Ribosom_L13_bac. 1 hit.
PTHR11545. Ribosomal_L13. 1 hit.

Pfam

PF00572. Ribosomal_L13. 1 hit.
[Graphical view]

PIRSF

PIRSF002181. Ribosomal_L13. 1 hit.

ProDom

PD001791. Ribosomal_L13. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01066. rplM_bact. 1 hit.

PROSITE

PS00783. RIBOSOMAL_L13. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL13_ECOLI

Accession

Primary (citable) accession number: P0AA10
Secondary accession number(s): P02410, Q2M8Y2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)