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Protein

Phosphocarrier protein HPr

Gene

ptsH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the permease (enzymes II/III).

Catalytic activityi

Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei15Pros-phosphohistidine intermediatePROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

  • enzyme activator activity Source: EcoliWiki
  • kinase activity Source: EcoCyc
  • phosphoenolpyruvate-protein phosphotransferase activity Source: EcoCyc
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
  • positive regulation of glycogen catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PTSH-MONOMER.
ECOL316407:JW2408-MONOMER.
MetaCyc:PTSH-MONOMER.

Protein family/group databases

TCDBi8.A.8.1.1. the phosphotransferase system hpr (hpr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphocarrier protein HPr (EC:2.7.11.-)
Alternative name(s):
Histidine-containing protein
Gene namesi
Name:ptsH
Synonyms:hpr
Ordered Locus Names:b2415, JW2408
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10788. ptsH.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001078501 – 85Phosphocarrier protein HPrAdd BLAST85

Proteomic databases

EPDiP0AA04.
PaxDbiP0AA04.
PRIDEiP0AA04.

2D gel databases

SWISS-2DPAGEP0AA04.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
bglGP119895EBI-902853,EBI-545674
ptsIP088392EBI-902853,EBI-551533

Protein-protein interaction databases

BioGridi4259700. 557 interactors.
DIPiDIP-35731N.
IntActiP0AA04. 7 interactors.
MINTiMINT-8085981.
STRINGi511145.b2415.

Structurei

Secondary structure

185
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Beta strandi10 – 12Combined sources3
Helixi16 – 26Combined sources11
Beta strandi31 – 37Combined sources7
Beta strandi40 – 43Combined sources4
Helixi47 – 50Combined sources4
Beta strandi60 – 67Combined sources8
Helixi70 – 81Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CM2X-ray1.80A1-85[»]
1CM3X-ray1.60A1-85[»]
1GGRNMR-B1-85[»]
1HDNNMR-A1-85[»]
1J6TNMR-B1-85[»]
1OPDX-ray1.50A1-85[»]
1PFHNMR-A1-85[»]
1POHX-ray2.00A1-85[»]
1VRCNMR-C/D1-85[»]
2JELX-ray2.50P1-85[»]
2LRKNMR-D1-85[»]
2LRLNMR-D1-85[»]
2XDFNMR-C/D1-85[»]
3CCDX-ray1.00A/B1-85[»]
3EZANMR-B1-85[»]
3EZBNMR-B1-85[»]
3EZENMR-B1-85[»]
4XWJX-ray2.10B1-85[»]
ProteinModelPortaliP0AA04.
SMRiP0AA04.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AA04.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 85HPrPROSITE-ProRule annotationAdd BLAST85

Sequence similaritiesi

Belongs to the HPr family.Curated
Contains 1 HPr domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1925. LUCA.
HOGENOMiHOG000278399.
InParanoidiP0AA04.
KOiK02784.
OMAiEKQAVEH.
PhylomeDBiP0AA04.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di3.30.1340.10. 1 hit.
InterProiIPR000032. HPr-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AA04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL
60 70 80
QTLGLTQGTV VTISAEGEDE QKAVEHLVKL MAELE
Length:85
Mass (Da):9,119
Last modified:April 1, 1988 - v1
Checksum:iE5C8879C0A95AD41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10425 Genomic DNA. Translation: AAA24438.1.
J02796 Genomic DNA. Translation: AAA24440.1.
M21994 Genomic DNA. Translation: AAA24384.1.
M21451 Genomic DNA. Translation: AAA23655.1.
U00096 Genomic DNA. Translation: AAC75468.1.
AP009048 Genomic DNA. Translation: BAA16289.1.
PIRiA29785. WQECPH.
RefSeqiNP_416910.1. NC_000913.3.
WP_000487600.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75468; AAC75468; b2415.
BAA16289; BAA16289; BAA16289.
GeneIDi5550362.
946886.
KEGGiecj:JW2408.
eco:b2415.
PATRICi32120213. VBIEscCol129921_2509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10425 Genomic DNA. Translation: AAA24438.1.
J02796 Genomic DNA. Translation: AAA24440.1.
M21994 Genomic DNA. Translation: AAA24384.1.
M21451 Genomic DNA. Translation: AAA23655.1.
U00096 Genomic DNA. Translation: AAC75468.1.
AP009048 Genomic DNA. Translation: BAA16289.1.
PIRiA29785. WQECPH.
RefSeqiNP_416910.1. NC_000913.3.
WP_000487600.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CM2X-ray1.80A1-85[»]
1CM3X-ray1.60A1-85[»]
1GGRNMR-B1-85[»]
1HDNNMR-A1-85[»]
1J6TNMR-B1-85[»]
1OPDX-ray1.50A1-85[»]
1PFHNMR-A1-85[»]
1POHX-ray2.00A1-85[»]
1VRCNMR-C/D1-85[»]
2JELX-ray2.50P1-85[»]
2LRKNMR-D1-85[»]
2LRLNMR-D1-85[»]
2XDFNMR-C/D1-85[»]
3CCDX-ray1.00A/B1-85[»]
3EZANMR-B1-85[»]
3EZBNMR-B1-85[»]
3EZENMR-B1-85[»]
4XWJX-ray2.10B1-85[»]
ProteinModelPortaliP0AA04.
SMRiP0AA04.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259700. 557 interactors.
DIPiDIP-35731N.
IntActiP0AA04. 7 interactors.
MINTiMINT-8085981.
STRINGi511145.b2415.

Protein family/group databases

TCDBi8.A.8.1.1. the phosphotransferase system hpr (hpr) family.

2D gel databases

SWISS-2DPAGEP0AA04.

Proteomic databases

EPDiP0AA04.
PaxDbiP0AA04.
PRIDEiP0AA04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75468; AAC75468; b2415.
BAA16289; BAA16289; BAA16289.
GeneIDi5550362.
946886.
KEGGiecj:JW2408.
eco:b2415.
PATRICi32120213. VBIEscCol129921_2509.

Organism-specific databases

EchoBASEiEB0781.
EcoGeneiEG10788. ptsH.

Phylogenomic databases

eggNOGiCOG1925. LUCA.
HOGENOMiHOG000278399.
InParanoidiP0AA04.
KOiK02784.
OMAiEKQAVEH.
PhylomeDBiP0AA04.

Enzyme and pathway databases

BioCyciEcoCyc:PTSH-MONOMER.
ECOL316407:JW2408-MONOMER.
MetaCyc:PTSH-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AA04.
PROiP0AA04.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di3.30.1340.10. 1 hit.
InterProiIPR000032. HPr-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTHP_ECOLI
AccessioniPrimary (citable) accession number: P0AA04
Secondary accession number(s): P05525, P07006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.