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P0A9Z8 (BLA2_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase SHV-2

EC=3.5.2.6
Alternative name(s):
SHV-2A
Gene names
Name:bla
Synonyms:shv2
Encoded onPlasmid pZMP1 Ref.2
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme hydrolyzes cefotaxime, ceftazidime and other broad spectrum cephalosporins.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 286265Beta-lactamase SHV-2
PRO_0000041957

Regions

Region230 – 2323Substrate binding By similarity

Sites

Active site661Acyl-ester intermediate By similarity
Active site1641Proton acceptor By similarity

Amino acid modifications

Disulfide bond73 ↔ 119 By similarity

Experimental info

Sequence conflict311Q → L in CAA44025. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0A9Z8 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 49BF426DBC1F551D

FASTA28631,269
        10         20         30         40         50         60 
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK QSESQLSGRV GMIEMDLASG RTLTAWRADE 

        70         80         90        100        110        120 
RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 

       130        140        150        160        170        180 
AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA 

       190        200        210        220        230        240 
SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASERGARG 

       250        260        270        280 
IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR 

« Hide

References

[1]"Direct involvement of IS26 in an antibiotic resistance operon."
Lee K.Y., Hopkins J.D., Syvanen M.
J. Bacteriol. 172:3229-3236(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Molecular characterization of a new plasmid-encoded SHV-type beta-lactamase (SHV-2 variant) conferring high-level cefotaxime resistance upon Klebsiella pneumoniae."
Podbielski A., Schoenling J., Melzer B., Warnatz K., Leusch H.G.
J. Gen. Microbiol. 137:569-578(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: KPR 14.
[3]"New system based on site-directed mutagenesis for highly accurate comparison of resistance levels conferred by SHV beta-lactamases."
Nuesch-Inderbinen M., Hachler H., Kayser F.H.
Antimicrob. Agents Chemother. 39:1726-1730(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: KPZU-3.
[4]"Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12."
Nuesch-Inderbinen M., Kayser F.H., Hachler H.
Antimicrob. Agents Chemother. 41:943-949(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: KPLA-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62115 Genomic DNA. Translation: CAA44025.1.
X53817 Genomic DNA. Translation: CAA37813.1.
X84314 Genomic DNA. Translation: CAA59058.1.
X98102 Genomic DNA. Translation: CAA66730.1.
RefSeqYP_001966240.1. NC_010886.1.

3D structure databases

ProteinModelPortalP0A9Z8.
SMRP0A9Z8. Positions 22-286.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6384172.

Phylogenomic databases

ProtClustDBPRK15442.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLA2_KLEPN
AccessionPrimary (citable) accession number: P0A9Z8
Secondary accession number(s): P14558
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: October 16, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families