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Protein

Beta-lactamase SHV-2

Gene

bla

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme hydrolyzes cefotaxime, ceftazidime and other broad spectrum cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei164 – 1641Proton acceptorBy similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase SHV-2 (EC:3.5.2.6)
Alternative name(s):
SHV-2A
Gene namesi
Name:bla
Synonyms:shv2
Encoded oniPlasmid pZMP11 Publication
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121By similarityAdd
BLAST
Chaini22 – 286265Beta-lactamase SHV-2PRO_0000041957Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi73 ↔ 119By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP0A9Z8.
SMRiP0A9Z8. Positions 22-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni230 – 2323Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK18699.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9Z8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK QSESQLSGRV GMIEMDLASG
60 70 80 90 100
RTLTAWRADE RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD
110 120 130 140 150
YSPVSEKHLA DGMTVGELCA AAITMSDNSA ANLLLATVGG PAGLTAFLRQ
160 170 180 190 200
IGDNVTRLDR WETELNEALP GDARDTTTPA SMAATLRKLL TSQRLSARSQ
210 220 230 240 250
RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASERGARG IVALLGPNNK
260 270 280
AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR
Length:286
Mass (Da):31,269
Last modified:September 13, 2005 - v1
Checksum:i49BF426DBC1F551D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311Q → L in CAA44025 (PubMed:2160941).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62115 Genomic DNA. Translation: CAA44025.1.
X53817 Genomic DNA. Translation: CAA37813.1.
X84314 Genomic DNA. Translation: CAA59058.1.
X98102 Genomic DNA. Translation: CAA66730.1.
RefSeqiYP_001966240.1. NC_010886.1.
YP_009073958.1. NG_034407.1.
YP_009074111.1. NG_034478.1.
YP_009078751.1. NG_035915.1.

Genome annotation databases

GeneIDi6384172.
KEGGipg:6384172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62115 Genomic DNA. Translation: CAA44025.1.
X53817 Genomic DNA. Translation: CAA37813.1.
X84314 Genomic DNA. Translation: CAA59058.1.
X98102 Genomic DNA. Translation: CAA66730.1.
RefSeqiYP_001966240.1. NC_010886.1.
YP_009073958.1. NG_034407.1.
YP_009074111.1. NG_034478.1.
YP_009078751.1. NG_035915.1.

3D structure databases

ProteinModelPortaliP0A9Z8.
SMRiP0A9Z8. Positions 22-286.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi6384172.
KEGGipg:6384172.

Phylogenomic databases

KOiK18699.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Direct involvement of IS26 in an antibiotic resistance operon."
    Lee K.Y., Hopkins J.D., Syvanen M.
    J. Bacteriol. 172:3229-3236(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular characterization of a new plasmid-encoded SHV-type beta-lactamase (SHV-2 variant) conferring high-level cefotaxime resistance upon Klebsiella pneumoniae."
    Podbielski A., Schoenling J., Melzer B., Warnatz K., Leusch H.G.
    J. Gen. Microbiol. 137:569-578(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KPR 14.
    Plasmid: pZMP1
  3. "New system based on site-directed mutagenesis for highly accurate comparison of resistance levels conferred by SHV beta-lactamases."
    Nuesch-Inderbinen M., Hachler H., Kayser F.H.
    Antimicrob. Agents Chemother. 39:1726-1730(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KPZU-3.
  4. "Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12."
    Nuesch-Inderbinen M., Kayser F.H., Hachler H.
    Antimicrob. Agents Chemother. 41:943-949(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KPLA-10.

Entry informationi

Entry nameiBLA2_KLEPN
AccessioniPrimary (citable) accession number: P0A9Z8
Secondary accession number(s): P14558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: April 1, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.