Reviewed, UniProtKB/Swiss-Prot P0A9Z8 (BLA2_KLEPN)

Names and origin

Protein names

Recommended name:
    Beta-lactamase SHV-2
    EC=3.5.2.6
Alternative name(s):
    SHV-2A

Gene names

Name:	bla
Synonyms:	shv2

Encoded on

Plasmid pZMP1 Ref.2

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme hydrolyzes cefotaxime, ceftazidime and other broad spectrum cephalosporins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Plasmid
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	By similarity
	Chain	22 – 286	265	Beta-lactamase SHV-2		PRO_0000041957
Regions
	Region	230 – 232	3	Substrate binding By similarity
Sites
	Active site	66	1	Acyl-ester intermediate By similarity
	Active site	164	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 119		By similarity
Experimental info
	Sequence conflict	31	1	Q → L in CAA44025. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A9Z8-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 49BF426DBC1F551D	FASTA	286	31,269
	10 20 30 40 50 60 MRYIRLCIIS LLATLPLAVH ASPQPLEQIK QSESQLSGRV GMIEMDLASG RTLTAWRADE 70 80 90 100 110 120 RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 130 140 150 160 170 180 AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA 190 200 210 220 230 240 SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASERGARG 250 260 270 280 IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR

References

[1]	"Direct involvement of IS26 in an antibiotic resistance operon." Lee K.Y., Hopkins J.D., Syvanen M. J. Bacteriol. 172:3229-3236(1990) [PubMed: 2160941] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular characterization of a new plasmid-encoded SHV-type beta-lactamase (SHV-2 variant) conferring high-level cefotaxime resistance upon Klebsiella pneumoniae." Podbielski A., Schoenling J., Melzer B., Warnatz K., Leusch H.G. J. Gen. Microbiol. 137:569-578(1991) [PubMed: 2033379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KPR 14.
[3]	"New system based on site-directed mutagenesis for highly accurate comparison of resistance levels conferred by SHV beta-lactamases." Nuesch-Inderbinen M., Hachler H., Kayser F.H. Antimicrob. Agents Chemother. 39:1726-1730(1995) [PubMed: 7486909] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KPZU-3.
[4]	"Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12." Nuesch-Inderbinen M., Kayser F.H., Hachler H. Antimicrob. Agents Chemother. 41:943-949(1997) [PubMed: 9145849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KPLA-10.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X62115 Genomic DNA. Translation: CAA44025.1. X53817 Genomic DNA. Translation: CAA37813.1. X84314 Genomic DNA. Translation: CAA59058.1. X98102 Genomic DNA. Translation: CAA66730.1.
RefSeq	YP_001966240.1.
3D structure databases
HSSP	HSSP built from PDB template 1N9B based on UniProtKB P30896.
SMR	P0A9Z8. Positions 22-286.
ModBase	Search...
Genome annotation databases
GeneID	6384172.
Enzyme and pathway databases
BRENDA	3.5.2.6. 758.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA2_KLEPN

Accession

Primary (citable) accession number: P0A9Z8
Secondary accession number(s): P14558

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	September 1, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)