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P0A9Z8

- BLA2_KLEPN

UniProt

P0A9Z8 - BLA2_KLEPN

Protein

Beta-lactamase SHV-2

Gene

bla

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    This enzyme hydrolyzes cefotaxime, ceftazidime and other broad spectrum cephalosporins.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei66 – 661Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei164 – 1641Proton acceptorBy similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase SHV-2 (EC:3.5.2.6)
    Alternative name(s):
    SHV-2A
    Gene namesi
    Name:bla
    Synonyms:shv2
    Encoded oniPlasmid pZMP11 Publication
    OrganismiKlebsiella pneumoniae
    Taxonomic identifieri573 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121By similarityAdd
    BLAST
    Chaini22 – 286265Beta-lactamase SHV-2PRO_0000041957Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi73 ↔ 119By similarity

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP0A9Z8.
    SMRiP0A9Z8. Positions 22-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni230 – 2323Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A9Z8-1 [UniParc]FASTAAdd to Basket

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    MRYIRLCIIS LLATLPLAVH ASPQPLEQIK QSESQLSGRV GMIEMDLASG    50
    RTLTAWRADE RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD 100
    YSPVSEKHLA DGMTVGELCA AAITMSDNSA ANLLLATVGG PAGLTAFLRQ 150
    IGDNVTRLDR WETELNEALP GDARDTTTPA SMAATLRKLL TSQRLSARSQ 200
    RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASERGARG IVALLGPNNK 250
    AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR 286
    Length:286
    Mass (Da):31,269
    Last modified:September 13, 2005 - v1
    Checksum:i49BF426DBC1F551D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti31 – 311Q → L in CAA44025. (PubMed:2160941)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62115 Genomic DNA. Translation: CAA44025.1.
    X53817 Genomic DNA. Translation: CAA37813.1.
    X84314 Genomic DNA. Translation: CAA59058.1.
    X98102 Genomic DNA. Translation: CAA66730.1.
    RefSeqiWP_012477587.1. NC_010886.1.
    YP_001966240.1. NC_010886.1.

    Genome annotation databases

    GeneIDi6384172.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62115 Genomic DNA. Translation: CAA44025.1 .
    X53817 Genomic DNA. Translation: CAA37813.1 .
    X84314 Genomic DNA. Translation: CAA59058.1 .
    X98102 Genomic DNA. Translation: CAA66730.1 .
    RefSeqi WP_012477587.1. NC_010886.1.
    YP_001966240.1. NC_010886.1.

    3D structure databases

    ProteinModelPortali P0A9Z8.
    SMRi P0A9Z8. Positions 22-286.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6384172.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Direct involvement of IS26 in an antibiotic resistance operon."
      Lee K.Y., Hopkins J.D., Syvanen M.
      J. Bacteriol. 172:3229-3236(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Molecular characterization of a new plasmid-encoded SHV-type beta-lactamase (SHV-2 variant) conferring high-level cefotaxime resistance upon Klebsiella pneumoniae."
      Podbielski A., Schoenling J., Melzer B., Warnatz K., Leusch H.G.
      J. Gen. Microbiol. 137:569-578(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: KPR 14.
      Plasmid: pZMP1
    3. "New system based on site-directed mutagenesis for highly accurate comparison of resistance levels conferred by SHV beta-lactamases."
      Nuesch-Inderbinen M., Hachler H., Kayser F.H.
      Antimicrob. Agents Chemother. 39:1726-1730(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: KPZU-3.
    4. "Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12."
      Nuesch-Inderbinen M., Kayser F.H., Hachler H.
      Antimicrob. Agents Chemother. 41:943-949(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: KPLA-10.

    Entry informationi

    Entry nameiBLA2_KLEPN
    AccessioniPrimary (citable) accession number: P0A9Z8
    Secondary accession number(s): P14558
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3