Reviewed, UniProtKB/Swiss-Prot P0A9Z7 (BLA2_ECOLX)

Names and origin

Protein names

Recommended name:
    Beta-lactamase SHV-2
    EC=3.5.2.6
Alternative name(s):
    SHV-2A

Gene names

Name:	bla
Synonyms:	shv2

Encoded on

Plasmid pBWH77 Ref.2

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme hydrolyzes cefotaxime, ceftazidime and other broad spectrum cephalosporins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.2
	Chain	22 – 286	265	Beta-lactamase SHV-2		PRO_0000016981
Regions
	Region	230 – 232	3	Substrate binding By similarity
Sites
	Active site	66	1	Acyl-ester intermediate By similarity
	Active site	164	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 119		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A9Z7-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 738F4266651F551A	FASTA	286	31,254
	10 20 30 40 50 60 MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE 70 80 90 100 110 120 RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 130 140 150 160 170 180 AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA 190 200 210 220 230 240 SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASERGARG 250 260 270 280 IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR

References

[1]	"Automated thermal cycling is superior to traditional methods for nucleotide sequencing of bla(SHV) genes." Bradford P.A. Antimicrob. Agents Chemother. 43:2960-2963(1999) [PubMed: 10582889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-204 / JC2926 pBP60-1.
[2]	"Single amino acid substitution between SHV-1 beta-lactamase and cefotaxime-hydrolyzing SHV-2 enzyme." Barthelemy M., Peduzzi J., Yaghlane H.B., Labia R. FEBS Lett. 231:217-220(1988) [PubMed: 3129309] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-286. Strain: A2302.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF148851 Genomic DNA. Translation: AAD37413.1.
PIR	S02434.
3D structure databases
HSSP	HSSP built from PDB template 1N9B based on UniProtKB P30896.
SMR	P0A9Z7. Positions 22-286.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 246.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA2_ECOLX

Accession

Primary (citable) accession number: P0A9Z7
Secondary accession number(s): P14558

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)