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P0A9Z7

- BLA2_ECOLX

UniProt

P0A9Z7 - BLA2_ECOLX

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Protein
Beta-lactamase SHV-2
Gene
bla, shv2
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

This enzyme hydrolyzes cefotaxime, ceftazidime and other broad spectrum cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Acyl-ester intermediate By similarity
Active sitei164 – 1641Proton acceptor By similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase SHV-2 (EC:3.5.2.6)
Alternative name(s):
SHV-2A
Gene namesi
Name:bla
Synonyms:shv2
Encoded oniPlasmid pBWH771 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 Publication
Add
BLAST
Chaini22 – 286265Beta-lactamase SHV-2
PRO_0000016981Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi73 ↔ 119 By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP0A9Z7.
SMRiP0A9Z7. Positions 22-286.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni230 – 2323Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9Z7-1 [UniParc]FASTAAdd to Basket

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MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG    50
RTLTAWRADE RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD 100
YSPVSEKHLA DGMTVGELCA AAITMSDNSA ANLLLATVGG PAGLTAFLRQ 150
IGDNVTRLDR WETELNEALP GDARDTTTPA SMAATLRKLL TSQRLSARSQ 200
RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASERGARG IVALLGPNNK 250
AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR 286
Length:286
Mass (Da):31,254
Last modified:September 13, 2005 - v1
Checksum:i738F4266651F551A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148851 Genomic DNA. Translation: AAD37413.1.
PIRiS02434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148851 Genomic DNA. Translation: AAD37413.1 .
PIRi S02434.

3D structure databases

ProteinModelPortali P0A9Z7.
SMRi P0A9Z7. Positions 22-286.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Automated thermal cycling is superior to traditional methods for nucleotide sequencing of bla(SHV) genes."
    Bradford P.A.
    Antimicrob. Agents Chemother. 43:2960-2963(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-204 / JC2926 pBP60-1.
  2. "Single amino acid substitution between SHV-1 beta-lactamase and cefotaxime-hydrolyzing SHV-2 enzyme."
    Barthelemy M., Peduzzi J., Yaghlane H.B., Labia R.
    FEBS Lett. 231:217-220(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-286.
    Strain: A2302.
    Plasmid: pBWH77

Entry informationi

Entry nameiBLA2_ECOLX
AccessioniPrimary (citable) accession number: P0A9Z7
Secondary accession number(s): P14558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: October 16, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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