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Protein

Nitrogen regulatory protein P-II 1

Gene

glnB

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

P-II indirectly controls the transcription of the glutamine synthetase gene (GlnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GlnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by GlnE, so activating the enzyme (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3383-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein P-II 1
Gene namesi
Name:glnB
Ordered Locus Names:Z3829, ECs3419
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 112112Nitrogen regulatory protein P-II 1PRO_0000139773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511O-UMP-tyrosinePROSITE-ProRule annotation

Post-translational modificationi

Uridylylated/deuridylylated by GlnD.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP0A9Z3.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

MINTiMINT-1235648.
STRINGi155864.Z3829.

Structurei

3D structure databases

ProteinModelPortaliP0A9Z3.
SMRiP0A9Z3. Positions 1-112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108YZA. Bacteria.
COG0347. LUCA.
HOGENOMiHOG000017847.
KOiK04751.
OMAiAIEVKGF.
OrthoDBiEOG69GZGV.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
IPR002332. N-reg_PII_urydylation_site.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PIRSFiPIRSF039144. GlnB. 1 hit.
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
PS00496. PII_GLNB_UMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9Z3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIDAIIKP FKLDDVREAL AEVGITGMTV TEVKGFGRQK GHTELYRGAE
60 70 80 90 100
YMVDFLPKVK IEIVVPDDIV DTCVDTIIRT AQTGKIGDGK IFVFDVARVI
110
RIRTGEEDDA AI
Length:112
Mass (Da):12,425
Last modified:September 13, 2005 - v1
Checksum:iD1A4158A2F225042
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57667.1.
BA000007 Genomic DNA. Translation: BAB36842.1.
PIRiC91056.
RefSeqiNP_311446.1. NC_002695.1.
WP_000717694.1. NZ_LPWC01000059.1.

Genome annotation databases

EnsemblBacteriaiAAG57667; AAG57667; Z3829.
BAB36842; BAB36842; BAB36842.
GeneIDi23339395.
914897.
KEGGiece:Z3829.
ecs:ECs3419.
PATRICi18356274. VBIEscCol44059_3332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57667.1.
BA000007 Genomic DNA. Translation: BAB36842.1.
PIRiC91056.
RefSeqiNP_311446.1. NC_002695.1.
WP_000717694.1. NZ_LPWC01000059.1.

3D structure databases

ProteinModelPortaliP0A9Z3.
SMRiP0A9Z3. Positions 1-112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1235648.
STRINGi155864.Z3829.

Proteomic databases

PRIDEiP0A9Z3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG57667; AAG57667; Z3829.
BAB36842; BAB36842; BAB36842.
GeneIDi23339395.
914897.
KEGGiece:Z3829.
ecs:ECs3419.
PATRICi18356274. VBIEscCol44059_3332.

Phylogenomic databases

eggNOGiENOG4108YZA. Bacteria.
COG0347. LUCA.
HOGENOMiHOG000017847.
KOiK04751.
OMAiAIEVKGF.
OrthoDBiEOG69GZGV.

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3383-MONOMER.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
IPR002332. N-reg_PII_urydylation_site.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PIRSFiPIRSF039144. GlnB. 1 hit.
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
PS00496. PII_GLNB_UMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiGLNB_ECO57
AccessioniPrimary (citable) accession number: P0A9Z3
Secondary accession number(s): P05826
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: May 11, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.