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Protein

Nitrogen regulatory protein P-II 1

Gene

glnB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GlnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by GlnE, so activating the enzyme.

GO - Molecular functioni

  • ATP binding Source: EcoCyc
  • enzyme regulator activity Source: EcoCyc
  • identical protein binding Source: IntAct
  • small molecule binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-PII.
ECOL316407:JW2537-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein P-II 1
Gene namesi
Name:glnB
Ordered Locus Names:b2553, JW2537
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10384. glnB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 112112Nitrogen regulatory protein P-II 1PRO_0000139771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511O-UMP-tyrosinePROSITE-ProRule annotation1 Publication

Post-translational modificationi

Uridylylated/deuridylylated by GlnD.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP0A9Z1.
PaxDbiP0A9Z1.
PRIDEiP0A9Z1.

Interactioni

Subunit structurei

Homotrimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-551053,EBI-551053
glnDP272493EBI-551053,EBI-552032
glnKP0AC553EBI-551053,EBI-559503
glnLP0AFB55EBI-551053,EBI-701156
rraBP0AF902EBI-551053,EBI-544031

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259201. 28 interactions.
DIPiDIP-35005N.
IntActiP0A9Z1. 18 interactions.
MINTiMINT-1235669.
STRINGi511145.b2553.

Structurei

Secondary structure

1
112
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi10 – 123Combined sources
Helixi13 – 2311Combined sources
Beta strandi29 – 357Combined sources
Beta strandi50 – 523Combined sources
Beta strandi56 – 6510Combined sources
Helixi67 – 693Combined sources
Helixi70 – 8112Combined sources
Beta strandi91 – 955Combined sources
Turni102 – 1043Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PILX-ray2.70A1-112[»]
2PIIX-ray1.90A1-112[»]
ProteinModelPortaliP0A9Z1.
SMRiP0A9Z1. Positions 1-112.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9Z1.

Family & Domainsi

Sequence similaritiesi

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0347. LUCA.
HOGENOMiHOG000017847.
InParanoidiP0A9Z1.
KOiK04751.
OMAiKIMLEIV.
PhylomeDBiP0A9Z1.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
IPR002332. N-reg_PII_urydylation_site.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PIRSFiPIRSF039144. GlnB. 1 hit.
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
PS00496. PII_GLNB_UMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9Z1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIDAIIKP FKLDDVREAL AEVGITGMTV TEVKGFGRQK GHTELYRGAE
60 70 80 90 100
YMVDFLPKVK IEIVVPDDIV DTCVDTIIRT AQTGKIGDGK IFVFDVARVI
110
RIRTGEEDDA AI
Length:112
Mass (Da):12,425
Last modified:September 13, 2005 - v1
Checksum:iD1A4158A2F225042
GO

Sequence cautioni

The sequence AAA23883 differs from that shown. Reason: Frameshift at positions 103 and 105. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191A → R in AAA23883 (PubMed:2885322).Curated
Sequence conflicti81 – 822AQ → E in AAA23883 (PubMed:2885322).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16778 Genomic DNA. Translation: AAA23883.1. Frameshift.
X58872 Genomic DNA. Translation: CAA41683.1.
S67014 Genomic DNA. Translation: AAB28779.1.
U00096 Genomic DNA. Translation: AAC75606.1.
AP009048 Genomic DNA. Translation: BAA16461.1.
Z21843 Genomic DNA. Translation: CAA79890.1.
PIRiC49940. RGECP2.
RefSeqiNP_417048.1. NC_000913.3.
WP_000717694.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75606; AAC75606; b2553.
BAA16461; BAA16461; BAA16461.
GeneIDi23339395.
947016.
KEGGiecj:JW2537.
eco:b2553.
PATRICi32120505. VBIEscCol129921_2655.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16778 Genomic DNA. Translation: AAA23883.1. Frameshift.
X58872 Genomic DNA. Translation: CAA41683.1.
S67014 Genomic DNA. Translation: AAB28779.1.
U00096 Genomic DNA. Translation: AAC75606.1.
AP009048 Genomic DNA. Translation: BAA16461.1.
Z21843 Genomic DNA. Translation: CAA79890.1.
PIRiC49940. RGECP2.
RefSeqiNP_417048.1. NC_000913.3.
WP_000717694.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PILX-ray2.70A1-112[»]
2PIIX-ray1.90A1-112[»]
ProteinModelPortaliP0A9Z1.
SMRiP0A9Z1. Positions 1-112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259201. 28 interactions.
DIPiDIP-35005N.
IntActiP0A9Z1. 18 interactions.
MINTiMINT-1235669.
STRINGi511145.b2553.

Proteomic databases

EPDiP0A9Z1.
PaxDbiP0A9Z1.
PRIDEiP0A9Z1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75606; AAC75606; b2553.
BAA16461; BAA16461; BAA16461.
GeneIDi23339395.
947016.
KEGGiecj:JW2537.
eco:b2553.
PATRICi32120505. VBIEscCol129921_2655.

Organism-specific databases

EchoBASEiEB0379.
EcoGeneiEG10384. glnB.

Phylogenomic databases

eggNOGiCOG0347. LUCA.
HOGENOMiHOG000017847.
InParanoidiP0A9Z1.
KOiK04751.
OMAiKIMLEIV.
PhylomeDBiP0A9Z1.

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-PII.
ECOL316407:JW2537-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A9Z1.
PROiP0A9Z1.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
IPR002332. N-reg_PII_urydylation_site.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PIRSFiPIRSF039144. GlnB. 1 hit.
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
PS00496. PII_GLNB_UMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLNB_ECOLI
AccessioniPrimary (citable) accession number: P0A9Z1
Secondary accession number(s): P05826
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.