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Protein

Cold shock protein CspA

Gene

cspA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to and stimulates the transcription of the CCAAT-containing, cold-shock-inducible promoters of the H-NS and GyrA proteins. Binds also to the inverted repeat 5'-ATTGG-3'.1 Publication

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • RNA binding Source: EcoliWiki
  • single-stranded DNA binding Source: EcoliWiki
  • single-stranded RNA binding Source: EcoliWiki
  • transcription antitermination factor activity, RNA binding Source: EcoliWiki

GO - Biological processi

  • negative regulation of DNA-templated transcription, termination Source: EcoliWiki
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • response to cold Source: EcoliWiki
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD03695.
ECOL316407:JW3525-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cold shock protein CspA
Short name:
CSP-A
Alternative name(s):
7.4 kDa cold shock protein
CS7.4
Gene namesi
Name:cspA
Synonyms:cspS
Ordered Locus Names:b3556, JW3525
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10166. cspA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 7069Cold shock protein CspAPRO_0000100233Add
BLAST

Proteomic databases

EPDiP0A9X9.
PaxDbiP0A9X9.
PRIDEiP0A9X9.

Expressioni

Inductioni

In response to low temperature.

Interactioni

Protein-protein interaction databases

BioGridi4262533. 8 interactions.
DIPiDIP-31862N.
IntActiP0A9X9. 42 interactions.
MINTiMINT-1220153.
STRINGi511145.b3556.

Structurei

Secondary structure

1
70
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 139Combined sources
Helixi14 – 163Combined sources
Beta strandi18 – 2710Combined sources
Beta strandi29 – 335Combined sources
Helixi34 – 363Combined sources
Turni40 – 423Combined sources
Beta strandi50 – 567Combined sources
Beta strandi59 – 613Combined sources
Beta strandi63 – 697Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MJCX-ray2.00A2-70[»]
2BH8X-ray1.90A/B2-36[»]
2L15NMR-A1-70[»]
3MEFNMR-A2-70[»]
ProteinModelPortaliP0A9X9.
SMRiP0A9X9. Positions 2-70.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9X9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 6761CSDAdd
BLAST

Sequence similaritiesi

Contains 1 CSD (cold-shock) domain.Curated

Phylogenomic databases

eggNOGiCOG1278. LUCA.
HOGENOMiHOG000070674.
InParanoidiP0A9X9.
KOiK03704.
OMAiAIQNEGY.
PhylomeDBiP0A9X9.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR019844. Cold-shock_CS.
IPR012156. Cold_shock_CspA.
IPR011129. Cold_shock_prot.
IPR002059. CSP_DNA-bd.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF00313. CSD. 1 hit.
[Graphical view]
PIRSFiPIRSF002599. Cold_shock_A. 1 hit.
PRINTSiPR00050. COLDSHOCK.
SMARTiSM00357. CSP. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS00352. COLD_SHOCK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGKMTGIVK WFNADKGFGF ITPDDGSKDV FVHFSAIQND GYKSLDEGQK
60 70
VSFTIESGAK GPAAGNVTSL
Length:70
Mass (Da):7,403
Last modified:January 23, 2007 - v2
Checksum:iCED47F00BF18A49B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30139 Genomic DNA. Translation: AAA23617.1.
U00039 Genomic DNA. Translation: AAB18533.1.
U00096 Genomic DNA. Translation: AAC76580.1.
AP009048 Genomic DNA. Translation: BAE77739.1.
U60035 Genomic DNA. Translation: AAC80239.1.
PIRiJH0201. OCECJ.
RefSeqiNP_418012.1. NC_000913.3.
WP_000014594.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76580; AAC76580; b3556.
BAE77739; BAE77739; BAE77739.
GeneIDi23335706.
948070.
KEGGiecj:JW3525.
eco:b3556.
PATRICi32122584. VBIEscCol129921_3669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30139 Genomic DNA. Translation: AAA23617.1.
U00039 Genomic DNA. Translation: AAB18533.1.
U00096 Genomic DNA. Translation: AAC76580.1.
AP009048 Genomic DNA. Translation: BAE77739.1.
U60035 Genomic DNA. Translation: AAC80239.1.
PIRiJH0201. OCECJ.
RefSeqiNP_418012.1. NC_000913.3.
WP_000014594.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MJCX-ray2.00A2-70[»]
2BH8X-ray1.90A/B2-36[»]
2L15NMR-A1-70[»]
3MEFNMR-A2-70[»]
ProteinModelPortaliP0A9X9.
SMRiP0A9X9. Positions 2-70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262533. 8 interactions.
DIPiDIP-31862N.
IntActiP0A9X9. 42 interactions.
MINTiMINT-1220153.
STRINGi511145.b3556.

Proteomic databases

EPDiP0A9X9.
PaxDbiP0A9X9.
PRIDEiP0A9X9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76580; AAC76580; b3556.
BAE77739; BAE77739; BAE77739.
GeneIDi23335706.
948070.
KEGGiecj:JW3525.
eco:b3556.
PATRICi32122584. VBIEscCol129921_3669.

Organism-specific databases

EchoBASEiEB0164.
EcoGeneiEG10166. cspA.

Phylogenomic databases

eggNOGiCOG1278. LUCA.
HOGENOMiHOG000070674.
InParanoidiP0A9X9.
KOiK03704.
OMAiAIQNEGY.
PhylomeDBiP0A9X9.

Enzyme and pathway databases

BioCyciEcoCyc:PD03695.
ECOL316407:JW3525-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A9X9.
PROiP0A9X9.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR019844. Cold-shock_CS.
IPR012156. Cold_shock_CspA.
IPR011129. Cold_shock_prot.
IPR002059. CSP_DNA-bd.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF00313. CSD. 1 hit.
[Graphical view]
PIRSFiPIRSF002599. Cold_shock_A. 1 hit.
PRINTSiPR00050. COLDSHOCK.
SMARTiSM00357. CSP. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS00352. COLD_SHOCK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSPA_ECOLI
AccessioniPrimary (citable) accession number: P0A9X9
Secondary accession number(s): P15277
, P37410, Q2M7L7, Q54170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.