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Protein

Cold shock protein CspA

Gene

cspA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to and stimulates the transcription of the CCAAT-containing, cold-shock-inducible promoters of the H-NS and GyrA proteins. Binds also to the inverted repeat 5'-ATTGG-3'.1 Publication

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • RNA binding Source: EcoliWiki
  • single-stranded DNA binding Source: EcoliWiki
  • single-stranded RNA binding Source: EcoliWiki
  • transcription antitermination factor activity, RNA binding Source: EcoliWiki

GO - Biological processi

  • negative regulation of DNA-templated transcription, termination Source: EcoliWiki
  • response to cold Source: EcoliWiki
  • transcription, DNA-templated Source: EcoCyc

Keywordsi

Molecular functionActivator, DNA-binding
Biological processStress response, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD03695

Names & Taxonomyi

Protein namesi
Recommended name:
Cold shock protein CspA
Short name:
CSP-A
Alternative name(s):
7.4 kDa cold shock protein
CS7.41 Publication
Gene namesi
Name:cspA
Synonyms:cspS
Ordered Locus Names:b3556, JW3525
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10166 cspA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001002332 – 70Cold shock protein CspAAdd BLAST69

Proteomic databases

EPDiP0A9X9
PaxDbiP0A9X9
PRIDEiP0A9X9

Expressioni

Inductioni

In response to low temperature (at protein level) (PubMed:2404279, PubMed:8898389).2 Publications

Interactioni

Protein-protein interaction databases

BioGridi426253315 interactors.
DIPiDIP-31862N
IntActiP0A9X9 42 interactors.
STRINGi316385.ECDH10B_3735

Structurei

Secondary structure

170
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 13Combined sources9
Helixi14 – 16Combined sources3
Beta strandi18 – 27Combined sources10
Beta strandi29 – 33Combined sources5
Helixi34 – 36Combined sources3
Turni40 – 42Combined sources3
Beta strandi50 – 56Combined sources7
Beta strandi59 – 61Combined sources3
Beta strandi63 – 69Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MJCX-ray2.00A2-70[»]
2BH8X-ray1.90A/B2-36[»]
2L15NMR-A1-70[»]
3MEFNMR-A2-70[»]
ProteinModelPortaliP0A9X9
SMRiP0A9X9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9X9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 67CSDAdd BLAST61

Phylogenomic databases

eggNOGiCOG1278 LUCA
HOGENOMiHOG000070674
InParanoidiP0A9X9
KOiK03704
OMAiHHSAINS
PhylomeDBiP0A9X9

Family and domain databases

CDDicd04458 CSP_CDS, 1 hit
InterProiView protein in InterPro
IPR019844 Cold-shock_CS
IPR012156 Cold_shock_CspA
IPR011129 CSD
IPR002059 CSP_DNA-bd
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF00313 CSD, 1 hit
PIRSFiPIRSF002599 Cold_shock_A, 1 hit
PRINTSiPR00050 COLDSHOCK
SMARTiView protein in SMART
SM00357 CSP, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
PROSITEiView protein in PROSITE
PS00352 CSD_1, 1 hit
PS51857 CSD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGKMTGIVK WFNADKGFGF ITPDDGSKDV FVHFSAIQND GYKSLDEGQK
60 70
VSFTIESGAK GPAAGNVTSL
Length:70
Mass (Da):7,403
Last modified:January 23, 2007 - v2
Checksum:iCED47F00BF18A49B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30139 Genomic DNA Translation: AAA23617.1
U00039 Genomic DNA Translation: AAB18533.1
U00096 Genomic DNA Translation: AAC76580.1
AP009048 Genomic DNA Translation: BAE77739.1
U60035 Genomic DNA Translation: AAC80239.1
PIRiJH0201 OCECJ
RefSeqiNP_418012.1, NC_000913.3
WP_000014594.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76580; AAC76580; b3556
BAE77739; BAE77739; BAE77739
GeneIDi32213729
948070
KEGGiecj:JW3525
eco:b3556
PATRICifig|1411691.4.peg.3158

Similar proteinsi

Entry informationi

Entry nameiCSPA_ECOLI
AccessioniPrimary (citable) accession number: P0A9X9
Secondary accession number(s): P15277
, P37410, Q2M7L7, Q54170
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome