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P0A9X4 (MREB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rod shape-determining protein MreB
Gene names
Name:mreB
Synonyms:envB, rodY
Ordered Locus Names:b3251, JW3220
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in formation of the rod shape of the cell. May act as a negative regulator of FtsI. Polymerization and bundle formation is enhanced by CbeA. Ref.4

Enzyme regulation

Polymerization is inhibited by toxins CbtA and CptA, as well as by A22 ([S-(3,4-dichlorobenzyl)isothiourea)] all of which cause cell rounding and eventual death. Inhibition by toxin CbtA is neutralized by cytoskeleton bundling-enhancing protein CbeA, inhibition by toxin CptA is neutralized by antitoxin CptB, while inhibition by A22 can be neutralized by overexpression of CbeA. Ref.6 Ref.7 Ref.8

Subunit structure

Interacts with FtsZ, toxins CbtA and CptA, and cytoskeleton bundling-enhancing protein CbeA. Ref.6 Ref.7 Ref.8

Sequence similarities

Belongs to the FtsA/MreB family.

Sequence caution

The sequence AAA58054.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

cbeAP763642EBI-371008,EBI-1126877

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 347347Rod shape-determining protein MreB
PRO_0000062759

Experimental info

Sequence conflict60 – 612KQ → NE in AAA83891. Ref.1
Sequence conflict2651T → I in AAA83891. Ref.1
Sequence conflict277 – 2782QC → HT in AAA83891. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0A9X4 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 1393696D8CDAEF93

FASTA34736,952
        10         20         30         40         50         60 
MLKKFRGMFS NDLSIDLGTA NTLIYVKGQG IVLNEPSVVA IRQDRAGSPK SVAAVGHDAK 

        70         80         90        100        110        120 
QMLGRTPGNI AAIRPMKDGV IADFFVTEKM LQHFIKQVHS NSFMRPSPRV LVCVPVGATQ 

       130        140        150        160        170        180 
VERRAIRESA QGAGAREVFL IEEPMAAAIG AGLPVSEATG SMVVDIGGGT TEVAVISLNG 

       190        200        210        220        230        240 
VVYSSSVRIG GDRFDEAIIN YVRRNYGSLI GEATAERIKH EIGSAYPGDE VREIEVRGRN 

       250        260        270        280        290        300 
LAEGVPRGFT LNSNEILEAL QEPLTGIVSA VMVALEQCPP ELASDISERG MVLTGGGALL 

       310        320        330        340 
RNLDRLLMEE TGIPVVVAED PLTCVARGGG KALEMIDMHG GDLFSEE 

« Hide

References

« Hide 'large scale' references
[1]"Determinations of the DNA sequence of the mreB gene and of the gene products of the mre region that function in formation of the rod shape of Escherichia coli cells."
Doi M., Wachi M., Ishino F., Tomioka S., Ito M., Sakagami Y., Suzuki A., Matsuhashi M.
J. Bacteriol. 170:4619-4624(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
Strain: K12.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"New mre genes mreC and mreD, responsible for formation of the rod shape of Escherichia coli cells."
Wachi M., Doi M., Okada Y., Matsuhashi M.
J. Bacteriol. 171:6511-6516(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 338-347, FUNCTION.
Strain: K12.
[5]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-12.
Strain: K12 / EMG2.
[6]"YeeV is an Escherichia coli toxin that inhibits cell division by targeting the cytoskeleton proteins, FtsZ and MreB."
Tan Q., Awano N., Inouye M.
Mol. Microbiol. 79:109-118(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, INTERACTION WITH FTSZ AND CBTA.
Strain: K12 / BW25113.
[7]"A novel membrane-bound toxin for cell division, CptA (YgfX), inhibits polymerization of cytoskeleton proteins, FtsZ and MreB, in Escherichia coli."
Masuda H., Tan Q., Awano N., Yamaguchi Y., Inouye M.
FEMS Microbiol. Lett. 328:174-181(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, INTERACTION WITH CPTA.
Strain: B / BL21-DE3 and K12 / BW25113.
[8]"YeeU enhances the bundling of cytoskeletal polymers of MreB and FtsZ, antagonizing the CbtA (YeeV) toxicity in Escherichia coli."
Masuda H., Tan Q., Awano N., Wu K.P., Inouye M.
Mol. Microbiol. 84:979-989(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, INTERACTION WITH CBEA.
Strain: K12 / BW25113.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22055 Genomic DNA. Translation: AAA83891.1.
U18997 Genomic DNA. Translation: AAA58054.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76283.2.
AP009048 Genomic DNA. Translation: BAE77293.1.
M31792 Genomic DNA. Translation: AAA24154.1.
RefSeqNP_417717.2. NC_000913.3.
YP_491434.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A9X4.
SMRP0A9X4. Positions 8-338.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-31874N.
IntActP0A9X4. 73 interactions.
MINTMINT-1222190.
STRING511145.b3251.

Proteomic databases

PaxDbP0A9X4.
PRIDEP0A9X4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76283; AAC76283; b3251.
BAE77293; BAE77293; BAE77293.
GeneID12932946.
948588.
KEGGecj:Y75_p3170.
eco:b3251.
PATRIC32121928. VBIEscCol129921_3348.

Organism-specific databases

EchoBASEEB0603.
EcoGeneEG10608. mreB.

Phylogenomic databases

eggNOGCOG1077.
HOGENOMHOG000019757.
KOK03569.
OMAINYIRRN.
OrthoDBEOG66QM00.
PhylomeDBP0A9X4.
ProtClustDBPRK13927.

Enzyme and pathway databases

BioCycEcoCyc:EG10608-MONOMER.
ECOL316407:JW3220-MONOMER.

Gene expression databases

GenevestigatorP0A9X4.

Family and domain databases

InterProIPR004753. MreB_Mrl.
[Graphical view]
PfamPF06723. MreB_Mbl. 1 hit.
[Graphical view]
PRINTSPR01652. SHAPEPROTEIN.
TIGRFAMsTIGR00904. mreB. 1 hit.
ProtoNetSearch...

Other

PROP0A9X4.

Entry information

Entry nameMREB_ECOLI
AccessionPrimary (citable) accession number: P0A9X4
Secondary accession number(s): P13519, P76678, Q2M8W3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene