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Protein

Rod shape-determining protein MreB

Gene

mreB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in formation of the rod shape of the cell. May act as a negative regulator of FtsI. Polymerization and bundle formation is enhanced by CbeA.1 Publication

Enzyme regulationi

Polymerization is inhibited by toxins CbtA and CptA, as well as by A22 ([S-(3,4-dichlorobenzyl)isothiourea)] all of which cause cell rounding and eventual death. Inhibition by toxin CbtA is neutralized by cytoskeleton bundling-enhancing protein CbeA, inhibition by toxin CptA is neutralized by antitoxin CptB, while inhibition by A22 can be neutralized by overexpression of CbeA.3 Publications

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

GO - Biological processi

  • cell morphogenesis Source: InterPro
  • FtsZ-dependent cytokinesis Source: EcoCyc
  • negative regulation of cell division Source: CACAO
  • regulation of cell shape Source: EcoCyc
  • regulation of chromosome segregation Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Cell shape

Enzyme and pathway databases

BioCyciEcoCyc:EG10608-MONOMER.
ECOL316407:JW3220-MONOMER.

Protein family/group databases

TCDBi9.B.157.1.3. the cell shape-determining mrebcd (mrebcd) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Rod shape-determining protein MreB
Gene namesi
Name:mreB
Synonyms:envB, rodY
Ordered Locus Names:b3251, JW3220
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10608. mreB.

Subcellular locationi

GO - Cellular componenti

  • cytoskeleton Source: EcoCyc
  • intrinsic component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000627591 – 347Rod shape-determining protein MreBAdd BLAST347

Proteomic databases

EPDiP0A9X4.
PaxDbiP0A9X4.
PRIDEiP0A9X4.

Interactioni

Subunit structurei

Interacts with FtsZ, toxins CbtA and CptA, and cytoskeleton bundling-enhancing protein CbeA.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-371008,EBI-371008
cbeAP763642EBI-371008,EBI-1126877
ftsZP0A9A66EBI-371008,EBI-370963

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261940. 252 interactors.
DIPiDIP-31874N.
IntActiP0A9X4. 79 interactors.
MINTiMINT-1222190.
STRINGi511145.b3251.

Structurei

3D structure databases

ProteinModelPortaliP0A9X4.
SMRiP0A9X4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FtsA/MreB family.Curated

Phylogenomic databases

eggNOGiCOG1077. LUCA.
HOGENOMiHOG000019757.
InParanoidiP0A9X4.
KOiK03569.
OMAiYIAEDPL.
PhylomeDBiP0A9X4.

Family and domain databases

CDDicd10225. MreB_like. 1 hit.
InterProiIPR004753. MreB.
[Graphical view]
PfamiPF06723. MreB_Mbl. 1 hit.
[Graphical view]
PRINTSiPR01652. SHAPEPROTEIN.
TIGRFAMsiTIGR00904. mreB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A9X4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKKFRGMFS NDLSIDLGTA NTLIYVKGQG IVLNEPSVVA IRQDRAGSPK
60 70 80 90 100
SVAAVGHDAK QMLGRTPGNI AAIRPMKDGV IADFFVTEKM LQHFIKQVHS
110 120 130 140 150
NSFMRPSPRV LVCVPVGATQ VERRAIRESA QGAGAREVFL IEEPMAAAIG
160 170 180 190 200
AGLPVSEATG SMVVDIGGGT TEVAVISLNG VVYSSSVRIG GDRFDEAIIN
210 220 230 240 250
YVRRNYGSLI GEATAERIKH EIGSAYPGDE VREIEVRGRN LAEGVPRGFT
260 270 280 290 300
LNSNEILEAL QEPLTGIVSA VMVALEQCPP ELASDISERG MVLTGGGALL
310 320 330 340
RNLDRLLMEE TGIPVVVAED PLTCVARGGG KALEMIDMHG GDLFSEE
Length:347
Mass (Da):36,952
Last modified:September 13, 2005 - v1
Checksum:i1393696D8CDAEF93
GO

Sequence cautioni

The sequence AAA58054 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60 – 61KQ → NE in AAA83891 (PubMed:3049542).Curated2
Sequence conflicti265T → I in AAA83891 (PubMed:3049542).Curated1
Sequence conflicti277 – 278QC → HT in AAA83891 (PubMed:3049542).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22055 Genomic DNA. Translation: AAA83891.1.
U18997 Genomic DNA. Translation: AAA58054.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76283.2.
AP009048 Genomic DNA. Translation: BAE77293.1.
M31792 Genomic DNA. Translation: AAA24154.1.
RefSeqiNP_417717.2. NC_000913.3.
WP_000913396.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76283; AAC76283; b3251.
BAE77293; BAE77293; BAE77293.
GeneIDi24909945.
948588.
KEGGiecj:JW3220.
eco:b3251.
PATRICi32121928. VBIEscCol129921_3348.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22055 Genomic DNA. Translation: AAA83891.1.
U18997 Genomic DNA. Translation: AAA58054.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76283.2.
AP009048 Genomic DNA. Translation: BAE77293.1.
M31792 Genomic DNA. Translation: AAA24154.1.
RefSeqiNP_417717.2. NC_000913.3.
WP_000913396.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9X4.
SMRiP0A9X4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261940. 252 interactors.
DIPiDIP-31874N.
IntActiP0A9X4. 79 interactors.
MINTiMINT-1222190.
STRINGi511145.b3251.

Protein family/group databases

TCDBi9.B.157.1.3. the cell shape-determining mrebcd (mrebcd) family.

Proteomic databases

EPDiP0A9X4.
PaxDbiP0A9X4.
PRIDEiP0A9X4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76283; AAC76283; b3251.
BAE77293; BAE77293; BAE77293.
GeneIDi24909945.
948588.
KEGGiecj:JW3220.
eco:b3251.
PATRICi32121928. VBIEscCol129921_3348.

Organism-specific databases

EchoBASEiEB0603.
EcoGeneiEG10608. mreB.

Phylogenomic databases

eggNOGiCOG1077. LUCA.
HOGENOMiHOG000019757.
InParanoidiP0A9X4.
KOiK03569.
OMAiYIAEDPL.
PhylomeDBiP0A9X4.

Enzyme and pathway databases

BioCyciEcoCyc:EG10608-MONOMER.
ECOL316407:JW3220-MONOMER.

Miscellaneous databases

PROiP0A9X4.

Family and domain databases

CDDicd10225. MreB_like. 1 hit.
InterProiIPR004753. MreB.
[Graphical view]
PfamiPF06723. MreB_Mbl. 1 hit.
[Graphical view]
PRINTSiPR01652. SHAPEPROTEIN.
TIGRFAMsiTIGR00904. mreB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMREB_ECOLI
AccessioniPrimary (citable) accession number: P0A9X4
Secondary accession number(s): P13519, P76678, Q2M8W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.