P0A9X1 (ZNUC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc import ATP-binding protein ZnuC
EC=3.6.3.-

Gene names

Name:	znuC
Synonyms:	yebM
Ordered Locus Names:	b1858, JW1847

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex ZnuABC involved in zinc import. Responsible for energy coupling to the transport system. Ref.5
Enzyme regulation	Inhibited by arsenate. Ref.5
Subunit structure	The complex is composed of two ATP-binding proteins (ZnuC), two transmembrane proteins (ZnuB) and a solute-binding protein (ZnuA) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Induction	Transcriptionally repressed by zur (zinc uptake regulator), in response to high extracellular zinc concentrations. Ref.5 Ref.6
Sequence similarities	Belongs to the ABC transporter superfamily. Zinc importer (TC 3.A.1.15.5) family. [View classification] Contains 1 ABC transporter domain.
Sequence caution	The sequence U38702 differs from that shown. Reason: Frameshift at position 188.

Ontologies

Keywords
Biological process	Ion transport Transport Zinc transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from electronic annotation. Source: InterPro zinc transporting ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Zinc import ATP-binding protein ZnuC		PRO_0000093130
Regions
	Domain	5 – 220	216	ABC transporter
	Nucleotide binding	37 – 44	8	ATP Potential
Experimental info
	Sequence conflict	80 – 83	4	TTLP → STLS in U38702. Ref.1
	Sequence conflict	135	1	L → W in U38702. Ref.1
	Sequence conflict	143	1	V → A in U38702. Ref.1
	Sequence conflict	155	1	G → W in U38702. Ref.1
	Sequence conflict	174 – 176	3	VLM → ALL in U38702. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A9X1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: F4BF845AC3C7904C	FASTA	251	27,867
	10 20 30 40 50 60 MTSLVSLENV SVSFGQRRVL SDVSLELKPG KILTLLGPNG AGKSTLVRVV LGLVTPDEGV 70 80 90 100 110 120 IKRNGKLRIG YVPQKLYLDT TLPLTVNRFL RLRPGTHKED ILPALKRVQA GHLINAPMQK 130 140 150 160 170 180 LSGGETQRVL LARALLNRPQ LLVLDEPTQG VDVNGQVALY DLIDQLRREL DCGVLMVSHD 190 200 210 220 230 240 LHLVMAKTDE VLCLNHHICC SGTPEVVSLH PEFISMFGPR GAEQLGIYRH HHNHRHDLQG 250 RIVLRRGNDR S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Robison K., O'Keeffe T., Church G.M. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / EMG2.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The ZnuABC high-affinity zinc uptake system and its regulator Zur in Escherichia coli." Patzer S.I., Hantke K. Mol. Microbiol. 28:1199-1210(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN ZINC TRANSPORT, INDUCTION, ATPASE ACTIVITY, ENZYME REGULATION. Strain: K12.
[6]	"Transcriptional response of Escherichia coli to external zinc." Yamamoto K., Ishihama A. J. Bacteriol. 187:6333-6340(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38702 Genomic DNA. No translation available. U00096 Genomic DNA. Translation: AAC74928.1. AP009048 Genomic DNA. Translation: BAA15666.1.
PIR	B64948.
RefSeq	NP_416372.1. NC_000913.2. YP_490120.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A9X1.
SMR	P0A9X1. Positions 5-230.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48234N.
IntAct	P0A9X1. 13 interactions.
STRING	511145.b1858.
Protein family/group databases
TCDB	3.A.1.15.5. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PaxDb	P0A9X1.
PRIDE	P0A9X1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74928; AAC74928; b1858. BAA15666; BAA15666; BAA15666.
GeneID	12934230. 946374.
KEGG	ecj:Y75_p1834. eco:b1858.
PATRIC	32119039. VBIEscCol129921_1937.
Organism-specific databases
EchoBASE	EB2931.
EcoGene	EG13132. znuC.
Phylogenomic databases
eggNOG	COG1121.
KO	K09817.
OMA	LCLNQHV.
ProtClustDB	PRK09544.
Enzyme and pathway databases
BioCyc	EcoCyc:ZNUC-MONOMER. ECOL316407:JW1847-MONOMER.
Gene expression databases
Genevestigator	P0A9X1.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017882. ABC_transptr_Zn_ATP-bd_ZnuC. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. False negative. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51298. ZNUC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ZNUC_ECOLI

Accession

Primary (citable) accession number: P0A9X1
Secondary accession number(s): P52648, P76285

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 29, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program