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Protein

Lipopolysaccharide export system ATP-binding protein LptB

Gene

lptB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex LptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. Probably responsible for energy coupling to the transport system.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 438ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • lipopolysaccharide-transporting ATPase activity Source: EcoCyc
  • protein complex binding Source: EcoCyc

GO - Biological processi

  • lipopolysaccharide transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YHBG-MONOMER.
ECOL316407:JW3168-MONOMER.
MetaCyc:YHBG-MONOMER.

Protein family/group databases

TCDBi1.B.42.1.2. the outer membrane lipopolysaccharide export porin (lps-ep) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide export system ATP-binding protein LptB (EC:3.6.3.-)
Gene namesi
Name:lptB
Synonyms:yhbG
Ordered Locus Names:b3201, JW3168
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11680. lptB.

Subcellular locationi

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: EcoCyc
  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Results in an earlier growth arrest and onset of cell lethality.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 241240Lipopolysaccharide export system ATP-binding protein LptBPRO_0000093178Add
BLAST

Proteomic databases

EPDiP0A9V1.
PaxDbiP0A9V1.

Expressioni

Inductioni

Transcriptionally regulated by sigma-E factor.1 Publication

Interactioni

Subunit structurei

Component of the lipopolysaccharide transport and assembly complex. The LptBFG transporter is composed of two ATP-binding proteins (LptB) and two transmembrane proteins (LptF and LptG).1 Publication

GO - Molecular functioni

  • protein complex binding Source: EcoCyc

Protein-protein interaction databases

DIPiDIP-31876N.
IntActiP0A9V1. 4 interactions.
MINTiMINT-7137019.
STRINGi511145.b3201.

Structurei

Secondary structure

1
241
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 1311Combined sources
Beta strandi16 – 2712Combined sources
Beta strandi31 – 355Combined sources
Helixi42 – 509Combined sources
Beta strandi56 – 627Combined sources
Helixi72 – 776Combined sources
Beta strandi80 – 834Combined sources
Helixi95 – 1039Combined sources
Helixi111 – 12414Combined sources
Helixi128 – 1303Combined sources
Helixi135 – 1373Combined sources
Helixi140 – 15213Combined sources
Beta strandi157 – 1626Combined sources
Turni164 – 1674Combined sources
Helixi170 – 18516Combined sources
Beta strandi189 – 1957Combined sources
Helixi197 – 2015Combined sources
Beta strandi205 – 2117Combined sources
Beta strandi214 – 2196Combined sources
Helixi221 – 2255Combined sources
Helixi228 – 2314Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P31X-ray2.05A/B2-241[»]
4P32X-ray1.55A/B2-241[»]
4P33X-ray1.65A/B2-241[»]
ProteinModelPortaliP0A9V1.
SMRiP0A9V1. Positions 2-235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 237234ABC transporterPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108IUZ. Bacteria.
COG1137. LUCA.
InParanoidiP0A9V1.
KOiK06861.
OMAiYYLGDNF.
OrthoDBiEOG6VXF80.
PhylomeDBiP0A9V1.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR032823. BCA_ABC_TP_C.
IPR030921. LPS_export_LptB.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF12399. BCA_ABC_TP_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR04406. LPS_export_lptB. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9V1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLTAKNLA KAYKGRRVVE DVSLTVNSGE IVGLLGPNGA GKTTTFYMVV
60 70 80 90 100
GIVPRDAGNI IIDDDDISLL PLHARARRGI GYLPQEASIF RRLSVYDNLM
110 120 130 140 150
AVLQIRDDLS AEQREDRANE LMEEFHIEHL RDSMGQSLSG GERRRVEIAR
160 170 180 190 200
ALAANPKFIL LDEPFAGVDP ISVIDIKRII EHLRDSGLGV LITDHNVRET
210 220 230 240
LAVCERAYIV SQGHLIAHGT PTEILQDEHV KRVYLGEDFR L
Length:241
Mass (Da):26,801
Last modified:January 23, 2007 - v2
Checksum:i6041495BDDCDFA72
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 573RDA → ARC in AAB60162 (PubMed:8444818).Curated
Sequence conflicti55 – 573RDA → ARC in BAA02314 (PubMed:8444818).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12938 Genomic DNA. Translation: BAA02314.1.
U12684 Genomic DNA. Translation: AAB60162.1.
U18997 Genomic DNA. Translation: AAA58003.1.
U00096 Genomic DNA. Translation: AAC76233.1.
AP009048 Genomic DNA. Translation: BAE77245.1.
PIRiC65111.
RefSeqiNP_417668.1. NC_000913.3.
WP_000224099.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76233; AAC76233; b3201.
BAE77245; BAE77245; BAE77245.
GeneIDi947725.
KEGGiecj:JW3168.
eco:b3201.
PATRICi32121822. VBIEscCol129921_3295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12938 Genomic DNA. Translation: BAA02314.1.
U12684 Genomic DNA. Translation: AAB60162.1.
U18997 Genomic DNA. Translation: AAA58003.1.
U00096 Genomic DNA. Translation: AAC76233.1.
AP009048 Genomic DNA. Translation: BAE77245.1.
PIRiC65111.
RefSeqiNP_417668.1. NC_000913.3.
WP_000224099.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P31X-ray2.05A/B2-241[»]
4P32X-ray1.55A/B2-241[»]
4P33X-ray1.65A/B2-241[»]
ProteinModelPortaliP0A9V1.
SMRiP0A9V1. Positions 2-235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-31876N.
IntActiP0A9V1. 4 interactions.
MINTiMINT-7137019.
STRINGi511145.b3201.

Protein family/group databases

TCDBi1.B.42.1.2. the outer membrane lipopolysaccharide export porin (lps-ep) family.

Proteomic databases

EPDiP0A9V1.
PaxDbiP0A9V1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76233; AAC76233; b3201.
BAE77245; BAE77245; BAE77245.
GeneIDi947725.
KEGGiecj:JW3168.
eco:b3201.
PATRICi32121822. VBIEscCol129921_3295.

Organism-specific databases

EchoBASEiEB1631.
EcoGeneiEG11680. lptB.

Phylogenomic databases

eggNOGiENOG4108IUZ. Bacteria.
COG1137. LUCA.
InParanoidiP0A9V1.
KOiK06861.
OMAiYYLGDNF.
OrthoDBiEOG6VXF80.
PhylomeDBiP0A9V1.

Enzyme and pathway databases

BioCyciEcoCyc:YHBG-MONOMER.
ECOL316407:JW3168-MONOMER.
MetaCyc:YHBG-MONOMER.

Miscellaneous databases

PROiP0A9V1.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR032823. BCA_ABC_TP_C.
IPR030921. LPS_export_LptB.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF12399. BCA_ABC_TP_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR04406. LPS_export_lptB. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Physical map location of the rpoN gene of Escherichia coli."
    Imaishi H., Gomada M., Inouye S., Nakazawa A.
    J. Bacteriol. 175:1550-1551(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant."
    Powell B.S., Court D.L., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier M.H. Jr., Reizer J.
    J. Biol. Chem. 270:4822-4839(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-13.
    Strain: K12 / EMG2.
  6. Cited for: SUBCELLULAR LOCATION.
    Strain: BL21-DE3.
  7. "Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus."
    Sperandeo P., Pozzi C., Deho G., Polissi A.
    Res. Microbiol. 157:547-558(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN LPS BIOSYNTHESIS, DISRUPTION PHENOTYPE.
  8. "Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli."
    Sperandeo P., Cescutti R., Villa R., Di Benedetto C., Candia D., Deho G., Polissi A.
    J. Bacteriol. 189:244-253(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN LIPOPOLYSACCHARIDE TRANSPORT, INDUCTION.
  9. "Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli."
    Sperandeo P., Lau F.K., Carpentieri A., De Castro C., Molinaro A., Deho G., Silhavy T.J., Polissi A.
    J. Bacteriol. 190:4460-4469(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN LIPOPOLYSACCHARIDE TRANSPORT.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  10. "Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides."
    Narita S., Tokuda H.
    FEBS Lett. 583:2160-2164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH LPTF AND LPTG.

Entry informationi

Entry nameiLPTB_ECOLI
AccessioniPrimary (citable) accession number: P0A9V1
Secondary accession number(s): P31220, Q2M911
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: March 16, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.