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P0A9T0 (SERA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-3-phosphoglycerate dehydrogenase

Short name=PGDH
EC=1.1.1.95
Gene names
Name:serA
Ordered Locus Names:b2913, JW2880
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH.

2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.

Enzyme regulation

In bacteria displays feedback inhibition by L-serine.

Pathway

Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Contains 1 ACT domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 410409D-3-phosphoglycerate dehydrogenase
PRO_0000075999

Regions

Domain339 – 41072ACT
Nucleotide binding161 – 1622NAD By similarity
Nucleotide binding238 – 2403NAD By similarity
Nucleotide binding292 – 2954NAD By similarity

Sites

Active site2401
Active site2691
Active site2921Proton donor
Binding site1811NAD By similarity
Binding site2641NAD By similarity

Secondary structure

....................................................................... 410
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A9T0 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 61EF5EFC304DF6F0

FASTA41044,176
        10         20         30         40         50         60 
MAKVSLEKDK IKFLLVEGVH QKALESLRAA GYTNIEFHKG ALDDEQLKES IRDAHFIGLR 

        70         80         90        100        110        120 
SRTHLTEDVI NAAEKLVAIG CFCIGTNQVD LDAAAKRGIP VFNAPFSNTR SVAELVIGEL 

       130        140        150        160        170        180 
LLLLRGVPEA NAKAHRGVWN KLAAGSFEAR GKKLGIIGYG HIGTQLGILA ESLGMYVYFY 

       190        200        210        220        230        240 
DIENKLPLGN ATQVQHLSDL LNMSDVVSLH VPENPSTKNM MGAKEISLMK PGSLLINASR 

       250        260        270        280        290        300 
GTVVDIPALC DALASKHLAG AAIDVFPTEP ATNSDPFTSP LCEFDNVLLT PHIGGSTQEA 

       310        320        330        340        350        360 
QENIGLEVAG KLIKYSDNGS TLSAVNFPEV SLPLHGGRRL MHIHENRPGV LTALNKIFAE 

       370        380        390        400        410 
QGVNIAAQYL QTSAQMGYVV IDIEADEDVA EKALQAMKAI PGTIRARLLY 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of the serA gene of Escherichia coli and the amino acid sequence of the encoded protein, D-3-phosphoglycerate dehydrogenase."
Tobey K.L., Grant G.A.
J. Biol. Chem. 261:12179-12183(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]Roy I., Leadlay P.F.
Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-99.
Strain: K12.
[5]"The tdh and serA operons of Escherichia coli: mutational analysis of the regulatory elements of leucine-responsive genes."
Rex J.H., Aronson B.D., Somerville R.L.
J. Bacteriol. 173:5944-5953(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
Strain: K12.
[6]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-13.
Strain: K12 / EMG2.
[7]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[8]"The allosteric ligand site in the Vmax-type cooperative enzyme phosphoglycerate dehydrogenase."
Schuller D.J., Grant G.A., Banaszak L.J.
Nat. Struct. Biol. 2:69-76(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L29397 Genomic DNA. Translation: AAA24625.1.
U28377 Genomic DNA. Translation: AAA69080.1.
U00096 Genomic DNA. Translation: AAC75950.1.
AP009048 Genomic DNA. Translation: BAE76977.1.
X66836 Genomic DNA. Translation: CAA47308.1.
M64630 Genomic DNA. Translation: AAA73016.1.
PIRDEECPG. A25200.
RefSeqNP_417388.1. NC_000913.3.
YP_491113.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PSDX-ray2.75A/B2-410[»]
1SC6X-ray2.09A/B/C/D7-409[»]
1YBAX-ray2.24A/B/C/D1-410[»]
2P9CX-ray2.46A/B1-410[»]
2P9EX-ray2.60A/B/C/D1-410[»]
2P9GX-ray2.80A/B1-410[»]
2PA3X-ray2.74A1-410[»]
ProteinModelPortalP0A9T0.
SMRP0A9T0. Positions 5-410.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10851N.
IntActP0A9T0. 3 interactions.
STRING511145.b2913.

PTM databases

PhosSiteP0810437.

Proteomic databases

PaxDbP0A9T0.
PRIDEP0A9T0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75950; AAC75950; b2913.
BAE76977; BAE76977; BAE76977.
GeneID12932179.
945258.
KEGGecj:Y75_p2844.
eco:b2913.
PATRIC32121238. VBIEscCol129921_3007.

Organism-specific databases

EchoBASEEB0937.
EcoGeneEG10944. serA.

Phylogenomic databases

eggNOGCOG0111.
HOGENOMHOG000136696.
KOK00058.
OMATWNKVSA.
OrthoDBEOG6VXFC3.
PhylomeDBP0A9T0.
ProtClustDBPRK11790.

Enzyme and pathway databases

BioCycEcoCyc:PGLYCDEHYDROG-MONOMER.
ECOL316407:JW2880-MONOMER.
MetaCyc:PGLYCDEHYDROG-MONOMER.
SABIO-RKP0A9T0.
UniPathwayUPA00135; UER00196.

Gene expression databases

GenevestigatorP0A9T0.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR002912. ACT_dom.
IPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
PF01842. ACT. 1 hit.
[Graphical view]
PROSITEPS51671. ACT. 1 hit.
PS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0A9T0.
PROP0A9T0.

Entry information

Entry nameSERA_ECOLI
AccessionPrimary (citable) accession number: P0A9T0
Secondary accession number(s): P08328, Q2M9S9, Q47633
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene