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Protein

D-3-phosphoglycerate dehydrogenase

Gene

serA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH.
2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.

Enzyme regulationi

In bacteria displays feedback inhibition by L-serine.

Pathwayi: L-serine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. D-3-phosphoglycerate dehydrogenase (serA)
  2. Phosphoserine aminotransferase (serC)
  3. Phosphoserine phosphatase (serB)
This subpathway is part of the pathway L-serine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei181 – 1811NAD1 Publication
Active sitei240 – 2401
Binding sitei264 – 2641NAD1 Publication
Active sitei269 – 2691
Active sitei292 – 2921Proton donor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi161 – 1622NAD1 Publication
Nucleotide bindingi238 – 2403NAD1 Publication
Nucleotide bindingi292 – 2954NAD1 Publication

GO - Molecular functioni

  • 2-hydroxyglutarate dehydrogenase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • NAD binding Source: InterPro
  • phosphoglycerate dehydrogenase activity Source: EcoCyc
  • serine binding Source: EcoCyc

GO - Biological processi

  • L-serine biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Serine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:PGLYCDEHYDROG-MONOMER.
ECOL316407:JW2880-MONOMER.
MetaCyc:PGLYCDEHYDROG-MONOMER.
BRENDAi1.1.1.95. 2026.
SABIO-RKP0A9T0.
UniPathwayiUPA00135; UER00196.

Names & Taxonomyi

Protein namesi
Recommended name:
D-3-phosphoglycerate dehydrogenase (EC:1.1.1.95)
Short name:
PGDH
Gene namesi
Name:serA
Ordered Locus Names:b2913, JW2880
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10944. serA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 410409D-3-phosphoglycerate dehydrogenasePRO_0000075999Add
BLAST

Proteomic databases

EPDiP0A9T0.
PaxDbiP0A9T0.
PRIDEiP0A9T0.

Interactioni

Subunit structurei

Homotetramer.

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261173. 13 interactions.
DIPiDIP-10851N.
IntActiP0A9T0. 3 interactions.
STRINGi511145.b2913.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni6 – 83Combined sources
Beta strandi9 – 113Combined sources
Beta strandi13 – 153Combined sources
Helixi21 – 299Combined sources
Beta strandi35 – 373Combined sources
Helixi44 – 507Combined sources
Beta strandi55 – 595Combined sources
Beta strandi61 – 633Combined sources
Helixi67 – 726Combined sources
Beta strandi78 – 814Combined sources
Helixi91 – 966Combined sources
Turni105 – 1084Combined sources
Helixi109 – 12517Combined sources
Helixi127 – 13610Combined sources
Helixi143 – 1453Combined sources
Beta strandi153 – 1575Combined sources
Helixi161 – 17212Combined sources
Beta strandi176 – 1805Combined sources
Helixi197 – 2037Combined sources
Beta strandi205 – 2095Combined sources
Turni215 – 2195Combined sources
Helixi223 – 2286Combined sources
Beta strandi233 – 2375Combined sources
Beta strandi241 – 2444Combined sources
Helixi246 – 2549Combined sources
Beta strandi257 – 2648Combined sources
Turni276 – 2783Combined sources
Helixi279 – 2813Combined sources
Beta strandi287 – 2904Combined sources
Helixi298 – 31821Combined sources
Beta strandi324 – 3274Combined sources
Beta strandi336 – 34611Combined sources
Helixi350 – 36011Combined sources
Beta strandi364 – 3729Combined sources
Beta strandi374 – 38411Combined sources
Helixi387 – 39812Combined sources
Beta strandi403 – 4108Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PSDX-ray2.75A/B2-410[»]
1SC6X-ray2.09A/B/C/D7-410[»]
1YBAX-ray2.24A/B/C/D1-410[»]
2P9CX-ray2.46A/B1-410[»]
2P9EX-ray2.60A/B/C/D1-410[»]
2P9GX-ray2.80A/B1-410[»]
2PA3X-ray2.74A1-410[»]
ProteinModelPortaliP0A9T0.
SMRiP0A9T0. Positions 5-410.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9T0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini339 – 41072ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108JQ1. Bacteria.
COG0111. LUCA.
HOGENOMiHOG000136696.
InParanoidiP0A9T0.
KOiK00058.
OMAiKFVKYSD.
PhylomeDBiP0A9T0.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002912. ACT_dom.
IPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR029015. PGDH_2.
[Graphical view]
PANTHERiPTHR10996:SF18. PTHR10996:SF18. 1 hit.
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
PF01842. ACT. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9T0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVSLEKDK IKFLLVEGVH QKALESLRAA GYTNIEFHKG ALDDEQLKES
60 70 80 90 100
IRDAHFIGLR SRTHLTEDVI NAAEKLVAIG CFCIGTNQVD LDAAAKRGIP
110 120 130 140 150
VFNAPFSNTR SVAELVIGEL LLLLRGVPEA NAKAHRGVWN KLAAGSFEAR
160 170 180 190 200
GKKLGIIGYG HIGTQLGILA ESLGMYVYFY DIENKLPLGN ATQVQHLSDL
210 220 230 240 250
LNMSDVVSLH VPENPSTKNM MGAKEISLMK PGSLLINASR GTVVDIPALC
260 270 280 290 300
DALASKHLAG AAIDVFPTEP ATNSDPFTSP LCEFDNVLLT PHIGGSTQEA
310 320 330 340 350
QENIGLEVAG KLIKYSDNGS TLSAVNFPEV SLPLHGGRRL MHIHENRPGV
360 370 380 390 400
LTALNKIFAE QGVNIAAQYL QTSAQMGYVV IDIEADEDVA EKALQAMKAI
410
PGTIRARLLY
Length:410
Mass (Da):44,176
Last modified:January 23, 2007 - v2
Checksum:i61EF5EFC304DF6F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29397 Genomic DNA. Translation: AAA24625.1.
U28377 Genomic DNA. Translation: AAA69080.1.
U00096 Genomic DNA. Translation: AAC75950.1.
AP009048 Genomic DNA. Translation: BAE76977.1.
X66836 Genomic DNA. Translation: CAA47308.1.
M64630 Genomic DNA. Translation: AAA73016.1.
PIRiA25200. DEECPG.
RefSeqiNP_417388.1. NC_000913.3.
WP_001151604.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75950; AAC75950; b2913.
BAE76977; BAE76977; BAE76977.
GeneIDi945258.
KEGGiecj:JW2880.
eco:b2913.
PATRICi32121238. VBIEscCol129921_3007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29397 Genomic DNA. Translation: AAA24625.1.
U28377 Genomic DNA. Translation: AAA69080.1.
U00096 Genomic DNA. Translation: AAC75950.1.
AP009048 Genomic DNA. Translation: BAE76977.1.
X66836 Genomic DNA. Translation: CAA47308.1.
M64630 Genomic DNA. Translation: AAA73016.1.
PIRiA25200. DEECPG.
RefSeqiNP_417388.1. NC_000913.3.
WP_001151604.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PSDX-ray2.75A/B2-410[»]
1SC6X-ray2.09A/B/C/D7-410[»]
1YBAX-ray2.24A/B/C/D1-410[»]
2P9CX-ray2.46A/B1-410[»]
2P9EX-ray2.60A/B/C/D1-410[»]
2P9GX-ray2.80A/B1-410[»]
2PA3X-ray2.74A1-410[»]
ProteinModelPortaliP0A9T0.
SMRiP0A9T0. Positions 5-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261173. 13 interactions.
DIPiDIP-10851N.
IntActiP0A9T0. 3 interactions.
STRINGi511145.b2913.

Proteomic databases

EPDiP0A9T0.
PaxDbiP0A9T0.
PRIDEiP0A9T0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75950; AAC75950; b2913.
BAE76977; BAE76977; BAE76977.
GeneIDi945258.
KEGGiecj:JW2880.
eco:b2913.
PATRICi32121238. VBIEscCol129921_3007.

Organism-specific databases

EchoBASEiEB0937.
EcoGeneiEG10944. serA.

Phylogenomic databases

eggNOGiENOG4108JQ1. Bacteria.
COG0111. LUCA.
HOGENOMiHOG000136696.
InParanoidiP0A9T0.
KOiK00058.
OMAiKFVKYSD.
PhylomeDBiP0A9T0.

Enzyme and pathway databases

UniPathwayiUPA00135; UER00196.
BioCyciEcoCyc:PGLYCDEHYDROG-MONOMER.
ECOL316407:JW2880-MONOMER.
MetaCyc:PGLYCDEHYDROG-MONOMER.
BRENDAi1.1.1.95. 2026.
SABIO-RKP0A9T0.

Miscellaneous databases

EvolutionaryTraceiP0A9T0.
PROiP0A9T0.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002912. ACT_dom.
IPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR029015. PGDH_2.
[Graphical view]
PANTHERiPTHR10996:SF18. PTHR10996:SF18. 1 hit.
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
PF01842. ACT. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSERA_ECOLI
AccessioniPrimary (citable) accession number: P0A9T0
Secondary accession number(s): P08328, Q2M9S9, Q47633
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.