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Protein

Galactitol-1-phosphate 5-dehydrogenase

Gene

gatD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts galactitol 1-phosphate to tagatose 6-phosphate.

Catalytic activityi

Galactitol-1-phosphate + NAD+ = L-tagatose 6-phosphate + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi38 – 381Zinc 1; catalyticBy similarity
Metal bindingi59 – 591Zinc 1; catalyticBy similarity
Metal bindingi89 – 891Zinc 2By similarity
Metal bindingi92 – 921Zinc 2By similarity
Metal bindingi95 – 951Zinc 2By similarity
Metal bindingi103 – 1031Zinc 2By similarity
Metal bindingi144 – 1441Zinc 1; catalyticBy similarity

GO - Molecular functioni

  • galactitol-1-phosphate 5-dehydrogenase activity Source: EcoCyc
  • identical protein binding Source: IntAct
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • galactitol catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Galactitol metabolism

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:GALACTITOLPDEHYD-MONOMER.
ECOL316407:JW2075-MONOMER.
MetaCyc:GALACTITOLPDEHYD-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactitol-1-phosphate 5-dehydrogenase (EC:1.1.1.251)
Gene namesi
Name:gatD
Ordered Locus Names:b2091, JW2075
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12417. gatD.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Galactitol-1-phosphate 5-dehydrogenasePRO_0000160879Add
BLAST

Proteomic databases

PaxDbiP0A9S3.
PRIDEiP0A9S3.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-1127517,EBI-1127517

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4260429. 16 interactions.
DIPiDIP-47890N.
IntActiP0A9S3. 16 interactions.
MINTiMINT-8389742.
STRINGi511145.b2091.

Structurei

Secondary structure

1
346
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi8 – 103Combined sources
Beta strandi11 – 166Combined sources
Beta strandi27 – 3610Combined sources
Helixi41 – 466Combined sources
Beta strandi50 – 556Combined sources
Beta strandi60 – 689Combined sources
Beta strandi80 – 834Combined sources
Helixi93 – 964Combined sources
Helixi100 – 1023Combined sources
Turni109 – 1113Combined sources
Beta strandi116 – 1249Combined sources
Helixi125 – 1273Combined sources
Beta strandi128 – 1303Combined sources
Helixi137 – 1426Combined sources
Helixi143 – 15513Combined sources
Beta strandi163 – 1675Combined sources
Helixi171 – 18212Combined sources
Beta strandi186 – 19510Combined sources
Helixi196 – 2038Combined sources
Beta strandi207 – 2115Combined sources
Turni212 – 2143Combined sources
Helixi217 – 2237Combined sources
Helixi224 – 2274Combined sources
Beta strandi229 – 2357Combined sources
Helixi240 – 24910Combined sources
Beta strandi255 – 2584Combined sources
Beta strandi266 – 2683Combined sources
Helixi270 – 2789Combined sources
Beta strandi282 – 2854Combined sources
Helixi297 – 30711Combined sources
Helixi314 – 3163Combined sources
Beta strandi317 – 3215Combined sources
Helixi323 – 33311Combined sources
Beta strandi340 – 3445Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4A2CX-ray1.87A/B1-346[»]
4UEJX-ray1.74A/B1-346[»]
4UEKX-ray1.90A/B1-346[»]
4UEOX-ray2.00A/B1-346[»]
ProteinModelPortaliP0A9S3.
SMRiP0A9S3. Positions 1-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107WTH. Bacteria.
COG1063. LUCA.
HOGENOMiHOG000294670.
InParanoidiP0A9S3.
KOiK00094.
OMAiTSINFAG.
PhylomeDBiP0A9S3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9S3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSVVNDTDG IVRVAESVIP EIKHQDEVRV KIASSGLCGS DLPRIFKNGA
60 70 80 90 100
HYYPITLGHE FSGYIDAVGS GVDDLHPGDA VACVPLLPCF TCPECLKGFY
110 120 130 140 150
SQCAKYDFIG SRRDGGFAEY IVVKRKNVFA LPTDMPIEDG AFIEPITVGL
160 170 180 190 200
HAFHLAQGCE NKNVIIIGAG TIGLLAIQCA VALGAKSVTA IDISSEKLAL
210 220 230 240 250
AKSFGAMQTF NSSEMSAPQM QSVLRELRFN QLILETAGVP QTVELAVEIA
260 270 280 290 300
GPHAQLALVG TLHQDLHLTS ATFGKILRKE LTVIGSWMNY SSPWPGQEWE
310 320 330 340
TASRLLTERK LSLEPLIAHR GSFESFAQAV RDIARNAMPG KVLLIP
Length:346
Mass (Da):37,390
Last modified:July 19, 2005 - v1
Checksum:iB35A2B004E5A4032
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti213 – 2131S → L in CAA56231 (PubMed:7772602).Curated
Sequence conflicti222 – 2221S → G in CAA56231 (PubMed:7772602).Curated
Sequence conflicti226 – 2272EL → DV in CAA56231 (PubMed:7772602).Curated
Sequence conflicti271 – 2711A → T in CAA56231 (PubMed:7772602).Curated
Sequence conflicti327 – 3293AQA → TQV in CAA56231 (PubMed:7772602).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79837 Genomic DNA. Translation: CAA56231.1.
U00096 Genomic DNA. Translation: AAC75152.1.
AP009048 Genomic DNA. Translation: BAA15954.1.
PIRiB64976.
RefSeqiNP_416594.1. NC_000913.3.
WP_000844219.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75152; AAC75152; b2091.
BAA15954; BAA15954; BAA15954.
GeneIDi946598.
KEGGiecj:JW2075.
eco:b2091.
PATRICi32119515. VBIEscCol129921_2168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79837 Genomic DNA. Translation: CAA56231.1.
U00096 Genomic DNA. Translation: AAC75152.1.
AP009048 Genomic DNA. Translation: BAA15954.1.
PIRiB64976.
RefSeqiNP_416594.1. NC_000913.3.
WP_000844219.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4A2CX-ray1.87A/B1-346[»]
4UEJX-ray1.74A/B1-346[»]
4UEKX-ray1.90A/B1-346[»]
4UEOX-ray2.00A/B1-346[»]
ProteinModelPortaliP0A9S3.
SMRiP0A9S3. Positions 1-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260429. 16 interactions.
DIPiDIP-47890N.
IntActiP0A9S3. 16 interactions.
MINTiMINT-8389742.
STRINGi511145.b2091.

Proteomic databases

PaxDbiP0A9S3.
PRIDEiP0A9S3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75152; AAC75152; b2091.
BAA15954; BAA15954; BAA15954.
GeneIDi946598.
KEGGiecj:JW2075.
eco:b2091.
PATRICi32119515. VBIEscCol129921_2168.

Organism-specific databases

EchoBASEiEB2316.
EcoGeneiEG12417. gatD.

Phylogenomic databases

eggNOGiENOG4107WTH. Bacteria.
COG1063. LUCA.
HOGENOMiHOG000294670.
InParanoidiP0A9S3.
KOiK00094.
OMAiTSINFAG.
PhylomeDBiP0A9S3.

Enzyme and pathway databases

BioCyciEcoCyc:GALACTITOLPDEHYD-MONOMER.
ECOL316407:JW2075-MONOMER.
MetaCyc:GALACTITOLPDEHYD-MONOMER.

Miscellaneous databases

PROiP0A9S3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATD_ECOLI
AccessioniPrimary (citable) accession number: P0A9S3
Secondary accession number(s): P37190, P76410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.