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P0A9S3 (GATD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Galactitol-1-phosphate 5-dehydrogenase
EC=1.1.1.251
Gene names
Name:gatD
Ordered Locus Names:b2091, JW2075
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts galactitol 1-phosphate to tagatose 6-phosphate.

Catalytic activity

Galactitol-1-phosphate + NAD+ = L-tagatose 6-phosphate + NADH.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Galactitol-1-phosphate 5-dehydrogenase
PRO_0000160879

Sites

Metal binding381Zinc 1; catalytic By similarity
Metal binding591Zinc 1; catalytic By similarity
Metal binding891Zinc 2 By similarity
Metal binding921Zinc 2 By similarity
Metal binding951Zinc 2 By similarity
Metal binding1031Zinc 2 By similarity
Metal binding1441Zinc 1; catalytic By similarity

Experimental info

Sequence conflict2131S → L in CAA56231. Ref.1
Sequence conflict2221S → G in CAA56231. Ref.1
Sequence conflict226 – 2272EL → DV in CAA56231. Ref.1
Sequence conflict2711A → T in CAA56231. Ref.1
Sequence conflict327 – 3293AQA → TQV in CAA56231. Ref.1

Secondary structure

1 ................................................................. 346
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A9S3 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: B35A2B004E5A4032

FASTA34637,390
        10         20         30         40         50         60 
MKSVVNDTDG IVRVAESVIP EIKHQDEVRV KIASSGLCGS DLPRIFKNGA HYYPITLGHE 

        70         80         90        100        110        120 
FSGYIDAVGS GVDDLHPGDA VACVPLLPCF TCPECLKGFY SQCAKYDFIG SRRDGGFAEY 

       130        140        150        160        170        180 
IVVKRKNVFA LPTDMPIEDG AFIEPITVGL HAFHLAQGCE NKNVIIIGAG TIGLLAIQCA 

       190        200        210        220        230        240 
VALGAKSVTA IDISSEKLAL AKSFGAMQTF NSSEMSAPQM QSVLRELRFN QLILETAGVP 

       250        260        270        280        290        300 
QTVELAVEIA GPHAQLALVG TLHQDLHLTS ATFGKILRKE LTVIGSWMNY SSPWPGQEWE 

       310        320        330        340 
TASRLLTERK LSLEPLIAHR GSFESFAQAV RDIARNAMPG KVLLIP

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the gat operon for galactitol utilization from a wild-type strain EC3132 of Escherichia coli."
Nobelmann B., Lengeler J.W.
Biochim. Biophys. Acta 1262:69-72(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: EC3132.
[2]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X79837 Genomic DNA. Translation: CAA56231.1.
U00096 Genomic DNA. Translation: AAC75152.1.
AP009048 Genomic DNA. Translation: BAA15954.1.
PIRB64976.
RefSeqNP_416594.1. NC_000913.2.
YP_490329.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4A2CX-ray1.87A/B1-346[»]
ProteinModelPortalP0A9S3.
SMRP0A9S3. Positions 1-346.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-47890N.
IntActP0A9S3. 16 interactions.
MINTMINT-8389742.
STRING511145.b2091.

Proteomic databases

PaxDbP0A9S3.
PRIDEP0A9S3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75152; AAC75152; b2091.
BAA15954; BAA15954; BAA15954.
GeneID12931435.
946598.
KEGGecj:Y75_p2052.
eco:b2091.
PATRIC32119515. VBIEscCol129921_2168.

Organism-specific databases

EchoBASEEB2316.
EcoGeneEG12417. gatD.

Phylogenomic databases

eggNOGCOG1063.
HOGENOMHOG000294670.
KOK00094.
OMAKGKVGFL.
ProtClustDBPRK10309.

Enzyme and pathway databases

BioCycEcoCyc:GALACTITOLPDEHYD-MONOMER.
ECOL316407:JW2075-MONOMER.
MetaCyc:GALACTITOLPDEHYD-MONOMER.

Gene expression databases

GenevestigatorP0A9S3.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProIPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11695. PTHR11695. 1 hit.
PfamPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMSSF50129. GroES_like. 1 hit.
PROSITEPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATD_ECOLI
AccessionPrimary (citable) accession number: P0A9S3
Secondary accession number(s): P37190, P76410
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents