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Protein

Galactitol 1-phosphate 5-dehydrogenase

Gene

gatD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts galactitol 1-phosphate to D-tagatose 6-phosphate.1 Publication

Catalytic activityi

Galactitol 1-phosphate + NAD+ = D-tagatose 6-phosphate + NADH.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi38Zinc 1; catalyticBy similarity1
Metal bindingi59Zinc 1; catalyticBy similarity1
Metal bindingi89Zinc 2By similarity1
Metal bindingi92Zinc 2By similarity1
Metal bindingi95Zinc 2By similarity1
Metal bindingi103Zinc 2By similarity1
Metal bindingi144Zinc 1; catalyticBy similarity1

GO - Molecular functioni

  • galactitol-1-phosphate 5-dehydrogenase activity Source: EcoCyc
  • identical protein binding Source: IntAct
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • galactitol catabolic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processGalactitol metabolism
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:GALACTITOLPDEHYD-MONOMER
MetaCyc:GALACTITOLPDEHYD-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Galactitol 1-phosphate 5-dehydrogenase (EC:1.1.1.2511 Publication)
Gene namesi
Name:gatD
Ordered Locus Names:b2091, JW2075
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12417 gatD

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001608791 – 346Galactitol 1-phosphate 5-dehydrogenaseAdd BLAST346

Proteomic databases

PaxDbiP0A9S3
PRIDEiP0A9S3

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-1127517,EBI-1127517

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4260429, 17 interactors
DIPiDIP-47890N
IntActiP0A9S3, 17 interactors
MINTiP0A9S3
STRINGi316385.ECDH10B_2244

Structurei

Secondary structure

1346
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi8 – 10Combined sources3
Beta strandi11 – 16Combined sources6
Beta strandi27 – 36Combined sources10
Helixi41 – 46Combined sources6
Beta strandi50 – 55Combined sources6
Beta strandi60 – 68Combined sources9
Beta strandi80 – 83Combined sources4
Helixi93 – 96Combined sources4
Helixi100 – 102Combined sources3
Turni109 – 111Combined sources3
Beta strandi116 – 124Combined sources9
Helixi125 – 127Combined sources3
Beta strandi128 – 130Combined sources3
Helixi137 – 142Combined sources6
Helixi143 – 155Combined sources13
Beta strandi163 – 167Combined sources5
Helixi171 – 182Combined sources12
Beta strandi186 – 195Combined sources10
Helixi196 – 203Combined sources8
Beta strandi207 – 211Combined sources5
Turni212 – 214Combined sources3
Helixi217 – 223Combined sources7
Helixi224 – 227Combined sources4
Beta strandi229 – 235Combined sources7
Helixi240 – 249Combined sources10
Beta strandi255 – 258Combined sources4
Beta strandi266 – 268Combined sources3
Helixi270 – 278Combined sources9
Beta strandi282 – 285Combined sources4
Helixi297 – 307Combined sources11
Helixi314 – 316Combined sources3
Beta strandi317 – 321Combined sources5
Helixi323 – 333Combined sources11
Beta strandi340 – 344Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4A2CX-ray1.87A/B1-346[»]
4UEJX-ray1.74A/B1-346[»]
4UEKX-ray1.90A/B1-346[»]
4UEOX-ray2.00A/B1-346[»]
ProteinModelPortaliP0A9S3
SMRiP0A9S3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107WTH Bacteria
COG1063 LUCA
HOGENOMiHOG000294670
InParanoidiP0A9S3
KOiK00094
OMAiTNCCLKA
PhylomeDBiP0A9S3

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequencei

Sequence statusi: Complete.

P0A9S3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSVVNDTDG IVRVAESVIP EIKHQDEVRV KIASSGLCGS DLPRIFKNGA
60 70 80 90 100
HYYPITLGHE FSGYIDAVGS GVDDLHPGDA VACVPLLPCF TCPECLKGFY
110 120 130 140 150
SQCAKYDFIG SRRDGGFAEY IVVKRKNVFA LPTDMPIEDG AFIEPITVGL
160 170 180 190 200
HAFHLAQGCE NKNVIIIGAG TIGLLAIQCA VALGAKSVTA IDISSEKLAL
210 220 230 240 250
AKSFGAMQTF NSSEMSAPQM QSVLRELRFN QLILETAGVP QTVELAVEIA
260 270 280 290 300
GPHAQLALVG TLHQDLHLTS ATFGKILRKE LTVIGSWMNY SSPWPGQEWE
310 320 330 340
TASRLLTERK LSLEPLIAHR GSFESFAQAV RDIARNAMPG KVLLIP
Length:346
Mass (Da):37,390
Last modified:July 19, 2005 - v1
Checksum:iB35A2B004E5A4032
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213S → L in CAA56231 (PubMed:7772602).Curated1
Sequence conflicti222S → G in CAA56231 (PubMed:7772602).Curated1
Sequence conflicti226 – 227EL → DV in CAA56231 (PubMed:7772602).Curated2
Sequence conflicti271A → T in CAA56231 (PubMed:7772602).Curated1
Sequence conflicti327 – 329AQA → TQV in CAA56231 (PubMed:7772602).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79837 Genomic DNA Translation: CAA56231.1
U00096 Genomic DNA Translation: AAC75152.1
AP009048 Genomic DNA Translation: BAA15954.1
PIRiB64976
RefSeqiNP_416594.1, NC_000913.3
WP_000844219.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75152; AAC75152; b2091
BAA15954; BAA15954; BAA15954
GeneIDi946598
KEGGiecj:JW2075
eco:b2091
PATRICifig|1411691.4.peg.159

Similar proteinsi

Entry informationi

Entry nameiGATD_ECOLI
AccessioniPrimary (citable) accession number: P0A9S3
Secondary accession number(s): P37190, P76410
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health