P0A9S2 (FUCO_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lactaldehyde reductase
EC=1.1.1.77

Alternative name(s):
Propanediol oxidoreductase

Gene names

Name:	fucO
Ordered Locus Names:	Z4116, ECs3659

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-propane-1,2-diol + NAD+ = (R)-lactaldehyde + NADH. (S)-propane-1,2-diol + NAD+ = (S)-lactaldehyde + NADH.
Cofactor	Iron By similarity.
Pathway	Carbohydrate degradation; L-fucose degradation.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.
Sequence caution	The sequence AAG57913.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAB37082.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Fucose metabolism
Ligand	Iron Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fucose catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	lactaldehyde reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Lactaldehyde reductase		PRO_0000087825
Regions
	Nucleotide binding	97 – 98	2	NAD By similarity
	Nucleotide binding	139 – 143	5	NAD By similarity
	Nucleotide binding	180 – 184	5	NAD By similarity
Sites
	Metal binding	195	1	Iron By similarity
	Metal binding	199	1	Iron; via tele nitrogen By similarity
	Metal binding	262	1	Iron; via tele nitrogen By similarity
	Metal binding	276	1	Iron; via tele nitrogen By similarity
	Binding site	38	1	NAD By similarity
	Binding site	70	1	NAD By similarity
	Binding site	150	1	NAD By similarity
	Binding site	161	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A9S2 [UniParc]. Last modified November 16, 2011. Version 2. Checksum: E4D927AC8142098B	FASTA	382	40,513
	10 20 30 40 50 60 MANRMILNET AWFGRGAVGA LTDEVKRRGY QKALIVTDKT LVQCGVVAKV TDKMDAAGLA 70 80 90 100 110 120 WAIYDGVVPN PTITVVKEGL GVFQNSGADY LIAIGGGSPQ DTCKAIGIIS NNPEFADVRS 130 140 150 160 170 180 LEGLSPTNKP SVPILAIPTT AGTAAEVTIN YVITDEEKRR KFVCVDPHDI PQVAFIDADM 190 200 210 220 230 240 MDGMPPALKA ATGVDALTHA IEGYITRGAW ALTDALHIKA IEIIAGALRG SVAGDKDAGE 250 260 270 280 290 300 EMALGQYVAG MGFSNVGLGL VHGMAHPLGA FYNTPHGVAN AILLPHVMRY NADFTGEKYR 310 320 330 340 350 360 DIARVMGVKV EGMSLEEARN AAVEAVFALN RDVGIPPHLR DVGVRKEDIP ALAQAALDDV 370 380 CTGGNPREAT LEDIVELYHT AW

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG57913.1. Different initiation. BA000007 Genomic DNA. Translation: BAB37082.1. Different initiation.
PIR	C91086. E85931.
RefSeq	NP_289354.1. NC_002655.2. NP_311686.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0A9S2.
SMR	P0A9S2. Positions 1-382.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z4116.
Protocols and materials databases
DNASU	958299.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG57913; AAG57913; Z4116. BAB37082; BAB37082; BAB37082.
GeneID	916534. 958299.
KEGG	ece:Z4116. ecs:ECs3659.
PATRIC	18356797. VBIEscCol44059_3581.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1454.
HOGENOM	HOG000243333.
KO	K00048.
OMA	TTKAAWE.
ProtClustDB	PRK10624.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-3623-MONOMER.
UniPathway	UPA00563.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. IPR013460. Lactal_redase. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
TIGRFAMs	TIGR02638. lactal_redase. 1 hit.
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUCO_ECO57

Accession

Primary (citable) accession number: P0A9S2
Secondary accession number(s): P11549

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	November 16, 2011
Last modified:	May 1, 2013
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program