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Protein

Lactaldehyde reductase

Gene

fucO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(R)-propane-1,2-diol + NAD+ = (R)-lactaldehyde + NADH.
(S)-propane-1,2-diol + NAD+ = (S)-lactaldehyde + NADH.

Cofactori

Fe cation1 Publication

Enzyme regulationi

Inhibited by Zn2+.1 Publication

Pathwayi: L-fucose degradation

This protein is involved in the pathway L-fucose degradation, which is part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the pathway L-fucose degradation and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38NAD1
Binding sitei70NAD1
Binding sitei150NAD1
Binding sitei161NAD1
Metal bindingi195Iron1
Metal bindingi199Iron; via tele nitrogen1
Metal bindingi262Iron; via tele nitrogen1
Metal bindingi276Iron; via tele nitrogen1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi97 – 98NAD2
Nucleotide bindingi139 – 143NAD5
Nucleotide bindingi180 – 184NAD5

GO - Molecular functioni

  • ferrous iron binding Source: EcoCyc
  • lactaldehyde reductase activity Source: EcoCyc

GO - Biological processi

  • glycol catabolic process Source: EcoCyc
  • L-fucose catabolic process Source: EcoCyc
  • propanediol metabolic process Source: EcoCyc
  • rhamnose catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Fucose metabolism

Keywords - Ligandi

Iron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciEcoCyc:LACTALDREDUCT-MONOMER.
ECOL316407:JW2770-MONOMER.
MetaCyc:LACTALDREDUCT-MONOMER.
UniPathwayiUPA00563.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactaldehyde reductase (EC:1.1.1.77)
Alternative name(s):
Propanediol oxidoreductase
Gene namesi
Name:fucO
Ordered Locus Names:b2799, JW2770
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10351. fucO.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 9MANRMILNE → M: Loss of enzyme activity, loss of dimerization. 1 Publication9
Mutagenesisi16G → D: No effect on enzyme activity. 1 Publication1
Mutagenesisi38D → G: Enzyme can now use NADP. 1 Publication1
Mutagenesisi96G → E: Loss of NAD binding and enzyme activity. 1 Publication1
Mutagenesisi195D → L: Complete loss of iron-binding. 1 Publication1
Mutagenesisi199H → A or F: Complete loss of iron-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878241 – 382Lactaldehyde reductaseAdd BLAST382

Proteomic databases

PaxDbiP0A9S1.
PRIDEiP0A9S1.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4259223. 91 interactors.
DIPiDIP-48076N.
IntActiP0A9S1. 5 interactors.
MINTiMINT-1223334.
STRINGi511145.b2799.

Structurei

Secondary structure

1382
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Beta strandi9 – 14Combined sources6
Helixi17 – 20Combined sources4
Helixi21 – 28Combined sources8
Beta strandi32 – 38Combined sources7
Helixi39 – 43Combined sources5
Helixi46 – 56Combined sources11
Beta strandi60 – 67Combined sources8
Helixi73 – 85Combined sources13
Beta strandi89 – 96Combined sources8
Helixi97 – 111Combined sources15
Helixi113 – 115Combined sources3
Helixi119 – 121Combined sources3
Beta strandi122 – 124Combined sources3
Beta strandi134 – 138Combined sources5
Beta strandi140 – 142Combined sources3
Turni145 – 147Combined sources3
Beta strandi149 – 155Combined sources7
Turni156 – 159Combined sources4
Beta strandi160 – 165Combined sources6
Helixi167 – 169Combined sources3
Beta strandi172 – 176Combined sources5
Helixi178 – 180Combined sources3
Turni181 – 183Combined sources3
Helixi186 – 205Combined sources20
Helixi211 – 232Combined sources22
Helixi236 – 256Combined sources21
Helixi260 – 272Combined sources13
Helixi276 – 291Combined sources16
Helixi292 – 294Combined sources3
Helixi298 – 305Combined sources8
Helixi315 – 333Combined sources19
Helixi339 – 342Combined sources4
Helixi346 – 348Combined sources3
Helixi349 – 358Combined sources10
Helixi360 – 364Combined sources5
Beta strandi365 – 367Combined sources3
Helixi371 – 382Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RRMX-ray1.60A/B1-382[»]
2BI4X-ray2.85A/B1-382[»]
2BL4X-ray2.85A/B1-382[»]
5BR4X-ray0.91A/B1-382[»]
ProteinModelPortaliP0A9S1.
SMRiP0A9S1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9S1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C0A. Bacteria.
COG1454. LUCA.
HOGENOMiHOG000243333.
InParanoidiP0A9S1.
KOiK00048.
OMAiDIVGLYQ.

Family and domain databases

InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR013460. Lactal_redase.
[Graphical view]
PfamiPF00465. Fe-ADH. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02638. lactal_redase. 1 hit.
PROSITEiPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANRMILNET AWFGRGAVGA LTDEVKRRGY QKALIVTDKT LVQCGVVAKV
60 70 80 90 100
TDKMDAAGLA WAIYDGVVPN PTITVVKEGL GVFQNSGADY LIAIGGGSPQ
110 120 130 140 150
DTCKAIGIIS NNPEFADVRS LEGLSPTNKP SVPILAIPTT AGTAAEVTIN
160 170 180 190 200
YVITDEEKRR KFVCVDPHDI PQVAFIDADM MDGMPPALKA ATGVDALTHA
210 220 230 240 250
IEGYITRGAW ALTDALHIKA IEIIAGALRG SVAGDKDAGE EMALGQYVAG
260 270 280 290 300
MGFSNVGLGL VHGMAHPLGA FYNTPHGVAN AILLPHVMRY NADFTGEKYR
310 320 330 340 350
DIARVMGVKV EGMSLEEARN AAVEAVFALN RDVGIPPHLR DVGVRKEDIP
360 370 380
ALAQAALDDV CTGGNPREAT LEDIVELYHT AW
Length:382
Mass (Da):40,513
Last modified:November 16, 2011 - v2
Checksum:iE4D927AC8142098B
GO

Sequence cautioni

The sequence AAA23824 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA23825 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAB40449 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE76871 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA33124 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti273N → T in CAA33124 (PubMed:2664711).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31059 Genomic DNA. Translation: AAA23824.1. Different initiation.
X15025 Genomic DNA. Translation: CAA33124.1. Different initiation.
M27177 Genomic DNA. Translation: AAA23825.1. Different initiation.
U29581 Genomic DNA. Translation: AAB40449.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75841.2.
AP009048 Genomic DNA. Translation: BAE76871.1. Different initiation.
L07763 Genomic DNA. No translation available.
PIRiA32883. RDECLA.
RefSeqiNP_417279.2. NC_000913.3.
WP_000013588.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75841; AAC75841; b2799.
BAE76871; BAE76871; BAE76871.
GeneIDi947273.
KEGGiecj:JW2770.
eco:b2799.
PATRICi32121014. VBIEscCol129921_2899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31059 Genomic DNA. Translation: AAA23824.1. Different initiation.
X15025 Genomic DNA. Translation: CAA33124.1. Different initiation.
M27177 Genomic DNA. Translation: AAA23825.1. Different initiation.
U29581 Genomic DNA. Translation: AAB40449.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75841.2.
AP009048 Genomic DNA. Translation: BAE76871.1. Different initiation.
L07763 Genomic DNA. No translation available.
PIRiA32883. RDECLA.
RefSeqiNP_417279.2. NC_000913.3.
WP_000013588.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RRMX-ray1.60A/B1-382[»]
2BI4X-ray2.85A/B1-382[»]
2BL4X-ray2.85A/B1-382[»]
5BR4X-ray0.91A/B1-382[»]
ProteinModelPortaliP0A9S1.
SMRiP0A9S1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259223. 91 interactors.
DIPiDIP-48076N.
IntActiP0A9S1. 5 interactors.
MINTiMINT-1223334.
STRINGi511145.b2799.

Proteomic databases

PaxDbiP0A9S1.
PRIDEiP0A9S1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75841; AAC75841; b2799.
BAE76871; BAE76871; BAE76871.
GeneIDi947273.
KEGGiecj:JW2770.
eco:b2799.
PATRICi32121014. VBIEscCol129921_2899.

Organism-specific databases

EchoBASEiEB0347.
EcoGeneiEG10351. fucO.

Phylogenomic databases

eggNOGiENOG4105C0A. Bacteria.
COG1454. LUCA.
HOGENOMiHOG000243333.
InParanoidiP0A9S1.
KOiK00048.
OMAiDIVGLYQ.

Enzyme and pathway databases

UniPathwayiUPA00563.
BioCyciEcoCyc:LACTALDREDUCT-MONOMER.
ECOL316407:JW2770-MONOMER.
MetaCyc:LACTALDREDUCT-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A9S1.
PROiP0A9S1.

Family and domain databases

InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR013460. Lactal_redase.
[Graphical view]
PfamiPF00465. Fe-ADH. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02638. lactal_redase. 1 hit.
PROSITEiPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUCO_ECOLI
AccessioniPrimary (citable) accession number: P0A9S1
Secondary accession number(s): P11549, Q2MA35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 16, 2011
Last modified: November 2, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.