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Protein

Aldehyde-alcohol dehydrogenase

Gene

adhE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme has three activities: ADH, ACDH, and PFL-deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent reaction.

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.
Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei246By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi422 – 427NADSequence analysis6

GO - Molecular functioni

  • acetaldehyde dehydrogenase (acetylating) activity Source: EcoliWiki
  • alcohol dehydrogenase (NAD) activity Source: EcoliWiki
  • metal ion binding Source: InterPro

GO - Biological processi

  • carbon utilization Source: InterPro
  • ethanol biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Iron, NAD

Enzyme and pathway databases

BioCyciEcoCyc:ADHE-MONOMER.
ECOL316407:JW1228-MONOMER.
MetaCyc:ADHE-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde-alcohol dehydrogenase
Including the following 3 domains:
Alcohol dehydrogenase (EC:1.1.1.1)
Short name:
ADH
Acetaldehyde dehydrogenase [acetylating] (EC:1.2.1.10)
Short name:
ACDH
Pyruvate-formate-lyase deactivase
Short name:
PFL deactivase
Gene namesi
Name:adhE
Synonyms:ana
Ordered Locus Names:b1241, JW1228
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10031. adhE.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved3 Publications
ChainiPRO_00000878372 – 891Aldehyde-alcohol dehydrogenaseAdd BLAST890

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei358N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0A9Q7.
PaxDbiP0A9Q7.
PRIDEiP0A9Q7.

Expressioni

Inductioni

Under anaerobic conditions in the absence of nitrate.

Interactioni

Subunit structurei

Seems to form a rod shaped polymer composed of about 40 identical subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-543417,EBI-543417

Protein-protein interaction databases

BioGridi4259605. 5 interactors.
850204. 1 interactor.
DIPiDIP-35790N.
IntActiP0A9Q7. 18 interactors.
MINTiMINT-1288364.
STRINGi511145.b1241.

Structurei

3D structure databases

ProteinModelPortaliP0A9Q7.
SMRiP0A9Q7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the aldehyde dehydrogenase family.Curated
In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105C0A. Bacteria.
COG1012. LUCA.
COG1454. LUCA.
HOGENOMiHOG000025256.
InParanoidiP0A9Q7.
KOiK04072.
OMAiFYEVEAD.
PhylomeDBiP0A9Q7.

Family and domain databases

Gene3Di3.40.309.10. 2 hits.
3.40.605.10. 1 hit.
InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012079. Bifunc_Ald-ADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
PF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFiPIRSF000111. ALDH_ADH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9Q7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVTNVAELN ALVERVKKAQ REYASFTQEQ VDKIFRAAAL AAADARIPLA
60 70 80 90 100
KMAVAESGMG IVEDKVIKNH FASEYIYNAY KDEKTCGVLS EDDTFGTITI
110 120 130 140 150
AEPIGIICGI VPTTNPTSTA IFKSLISLKT RNAIIFSPHP RAKDATNKAA
160 170 180 190 200
DIVLQAAIAA GAPKDLIGWI DQPSVELSNA LMHHPDINLI LATGGPGMVK
210 220 230 240 250
AAYSSGKPAI GVGAGNTPVV IDETADIKRA VASVLMSKTF DNGVICASEQ
260 270 280 290 300
SVVVVDSVYD AVRERFATHG GYLLQGKELK AVQDVILKNG ALNAAIVGQP
310 320 330 340 350
AYKIAELAGF SVPENTKILI GEVTVVDESE PFAHEKLSPT LAMYRAKDFE
360 370 380 390 400
DAVEKAEKLV AMGGIGHTSC LYTDQDNQPA RVSYFGQKMK TARILINTPA
410 420 430 440 450
SQGGIGDLYN FKLAPSLTLG CGSWGGNSIS ENVGPKHLIN KKTVAKRAEN
460 470 480 490 500
MLWHKLPKSI YFRRGSLPIA LDEVITDGHK RALIVTDRFL FNNGYADQIT
510 520 530 540 550
SVLKAAGVET EVFFEVEADP TLSIVRKGAE LANSFKPDVI IALGGGSPMD
560 570 580 590 600
AAKIMWVMYE HPETHFEELA LRFMDIRKRI YKFPKMGVKA KMIAVTTTSG
610 620 630 640 650
TGSEVTPFAV VTDDATGQKY PLADYALTPD MAIVDANLVM DMPKSLCAFG
660 670 680 690 700
GLDAVTHAME AYVSVLASEF SDGQALQALK LLKEYLPASY HEGSKNPVAR
710 720 730 740 750
ERVHSAATIA GIAFANAFLG VCHSMAHKLG SQFHIPHGLA NALLICNVIR
760 770 780 790 800
YNANDNPTKQ TAFSQYDRPQ ARRRYAEIAD HLGLSAPGDR TAAKIEKLLA
810 820 830 840 850
WLETLKAELG IPKSIREAGV QEADFLANVD KLSEDAFDDQ CTGANPRYPL
860 870 880 890
ISELKQILLD TYYGRDYVEG ETAAKKEAAP AKAEKKAKKS A
Length:891
Mass (Da):96,127
Last modified:January 23, 2007 - v2
Checksum:i75469F57419C8C79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59263 Genomic DNA. Translation: CAA41955.1.
M33504 Genomic DNA. Translation: AAA23420.1.
U00096 Genomic DNA. Translation: AAC74323.1.
AP009048 Genomic DNA. Translation: BAA36121.1.
X67326 Genomic DNA. Translation: CAA47743.1.
PIRiJS0406. DEEC.
RefSeqiNP_415757.1. NC_000913.3.
WP_000301651.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74323; AAC74323; b1241.
BAA36121; BAA36121; BAA36121.
GeneIDi945837.
KEGGiecj:JW1228.
eco:b1241.
PATRICi32117740. VBIEscCol129921_1290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59263 Genomic DNA. Translation: CAA41955.1.
M33504 Genomic DNA. Translation: AAA23420.1.
U00096 Genomic DNA. Translation: AAC74323.1.
AP009048 Genomic DNA. Translation: BAA36121.1.
X67326 Genomic DNA. Translation: CAA47743.1.
PIRiJS0406. DEEC.
RefSeqiNP_415757.1. NC_000913.3.
WP_000301651.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9Q7.
SMRiP0A9Q7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259605. 5 interactors.
850204. 1 interactor.
DIPiDIP-35790N.
IntActiP0A9Q7. 18 interactors.
MINTiMINT-1288364.
STRINGi511145.b1241.

Proteomic databases

EPDiP0A9Q7.
PaxDbiP0A9Q7.
PRIDEiP0A9Q7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74323; AAC74323; b1241.
BAA36121; BAA36121; BAA36121.
GeneIDi945837.
KEGGiecj:JW1228.
eco:b1241.
PATRICi32117740. VBIEscCol129921_1290.

Organism-specific databases

EchoBASEiEB0030.
EcoGeneiEG10031. adhE.

Phylogenomic databases

eggNOGiENOG4105C0A. Bacteria.
COG1012. LUCA.
COG1454. LUCA.
HOGENOMiHOG000025256.
InParanoidiP0A9Q7.
KOiK04072.
OMAiFYEVEAD.
PhylomeDBiP0A9Q7.

Enzyme and pathway databases

BioCyciEcoCyc:ADHE-MONOMER.
ECOL316407:JW1228-MONOMER.
MetaCyc:ADHE-MONOMER.

Miscellaneous databases

PROiP0A9Q7.

Family and domain databases

Gene3Di3.40.309.10. 2 hits.
3.40.605.10. 1 hit.
InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012079. Bifunc_Ald-ADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
PF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFiPIRSF000111. ALDH_ADH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADHE_ECOLI
AccessioniPrimary (citable) accession number: P0A9Q7
Secondary accession number(s): P17547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.