Aerobic respiration control protein ArcA - Escherichia coli (strain K12)

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P0A9Q1 (ARCA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Aerobic respiration control protein ArcA

Alternative name(s):
Dye resistance protein

Gene names

Name:	arcA
Synonyms:	cpxC, dye, fexA, msp, seg, sfrA
Ordered Locus Names:	b4401, JW4364

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system ArcB/ArcA. Represses a wide variety of aerobic enzymes under anaerobic conditions. Controls the resistance of E.coli to dyes; required for expression of the alkaline phosphatase and sex factor F genes; It also may be involved in the osmoregulation of envelope proteins. When activated by ArcB, it negatively regulates the expression of genes of aerobic function. Activates the transcription of the plfB operon by binding to its promoter.
Subcellular location	Cytoplasm Probable.
Post-translational modification	Phosphorylated by ArcB.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from direct assay PubMed 14711822 PubMed 2964639. Source: EcoCyc positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 14711822 PubMed 2964639. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from direct assay PubMed 1512197. Source: EcoCyc sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 14711822 PubMed 2964639. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-1119939,EBI-1119939
mexB	P52002	2	EBI-1119939,EBI-6400435	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 238

238

Aerobic respiration control protein ArcA

PRO_0000081008

Regions

Domain

1 – 118

118

Response regulatory

Amino acid modifications

Modified residue

4-aspartylphosphate By similarity

Secondary structure

238

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A9Q1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 9316CF4DE8EABDE8
Show »

FASTA

238

27,292

        10         20         30         40         50         60 
MQTPHILIVE DELVTRNTLK SIFEAEGYDV FEATDGAEMH QILSEYDINL VIMDINLPGK 

        70         80         90        100        110        120 
NGLLLARELR EQANVALMFL TGRDNEVDKI LGLEIGADDY ITKPFNPREL TIRARNLLSR 

       130        140        150        160        170        180 
TMNLGTVSEE RRSVESYKFN GWELDINSRS LIGPDGEQYK LPRSEFRAML HFCENPGKIQ 

       190        200        210        220        230 
SRAELLKKMT GRELKPHDRT VDVTIRRIRK HFESTPDTPE IIATIHGEGY RFCGDLED

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of the dye gene of Escherichia coli reveals amino acid homology between the dye and OmpR proteins." Drury L.S., Buxton R.S. J. Biol. Chem. 260:4236-4242(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	Park S.J., Gunsalus R.P. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23. Strain: K12.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
[7]	"Purification of ArcA and analysis of its specific interaction with the pfl promoter-regulatory region." Drapal N., Sawers G. Mol. Microbiol. 16:597-607(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 40-45 AND 123-128, CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M10044 Genomic DNA. Translation: AAA23718.1.
U14003 Genomic DNA. Translation: AAA97297.1.
U00096 Genomic DNA. Translation: AAC77354.1.
AP009048 Genomic DNA. Translation: BAE78390.1.
L34010 Genomic DNA. Translation: AAA23476.1.

PIR

JYECR. A03561.

RefSeq

NP_418818.1. NC_000913.2.
YP_492531.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XHE	X-ray	2.50	A/B	1-122	[»]
1XHF	X-ray	2.15	A/B	1-122	[»]

ProteinModelPortal

P0A9Q1.

SMR

P0A9Q1. Positions 1-234.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-36035N.

IntAct

P0A9Q1. 7 interactions.

STRING

511145.b4401.

2D gel databases

SWISS-2DPAGE

P0A9Q1.

Proteomic databases

PaxDb

P0A9Q1.

PRIDE

P0A9Q1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC77354; AAC77354; b4401.
BAE78390; BAE78390; BAE78390.

GeneID

12930392.
948874.

KEGG

ecj:Y75_p4285.
eco:b4401.

PATRIC

32124420. VBIEscCol129921_4550.

Organism-specific databases

EchoBASE

EB0059.

EcoGene

EG10061. arcA.

Phylogenomic databases

eggNOG

COG0745.

HOGENOM

HOG000034819.

K07773.

OMA

QRTMQEN.

ProtClustDB

PRK11173.

Enzyme and pathway databases

BioCyc

EcoCyc:ARCA-MONOMER.
ECOL316407:JW4364-MONOMER.

Gene expression databases

Genevestigator

P0A9Q1.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]

SMART

SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]

SUPFAM

SSF46894. Bipartite_resp_reg_C-effector. 1 hit.
SSF52172. CheY_like. 1 hit.

PROSITE

PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A9Q1.

Entry information

Entry name

ARCA_ECOLI

Accession

Primary (citable) accession number: P0A9Q1
Secondary accession number(s): P03026, Q2M5R6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents