Reviewed, UniProtKB/Swiss-Prot P0A9Q1 (ARCA_ECOLI)

Names and origin

Protein names

Recommended name:
    Aerobic respiration control protein arcA
Alternative name(s):
    Dye resistance protein

Gene names

Name:	arcA
Synonyms:	cpxC, dye, fexA, msp, seg, sfrA
Ordered Locus Names:	b4401, JW4364

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Member of the two-component regulatory system arcB/arcA. Represses a wide variety of aerobic enzymes under anaerobic conditions. Controls the resistance of E.coli to dyes; required for expression of the alkaline phosphatase and sex factor F genes; It also may be involved in the osmoregulation of envelope proteins. When activated by arcB, it negatively regulates the expression of genes of aerobic function. Activates the transcription of the plfB operon by binding to its promoter.
Subcellular location	Cytoplasm Probable.
Post-translational modification	Phosphorylated by arcB.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
prs	P0A717	1	EBI-1119939,EBI-906827

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 238

238

Aerobic respiration control protein arcA

PRO_0000081008

Regions

Domain

1 – 118

118

Response regulatory

Amino acid modifications

Modified residue

54

1

4-aspartylphosphate By similarity

Secondary structure

1

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238

Helix Strand Turn

Details...

Beta strand

5 – 9

5

Helix

13 – 24

12

Turn

25 – 27

3

Beta strand

29 – 35

7

Helix

36 – 45

10

Beta strand

49 – 53

5

Beta strand

58 – 60

3

Helix

62 – 72

11

Beta strand

76 – 82

7

Helix

86 – 95

10

Beta strand

98 – 104

7

Helix

107 – 121

15

Sequences

	Sequence		Length	Mass (Da)
	P0A9Q1-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 9316CF4DE8EABDE8	FASTA	238	27,292
	10 20 30 40 50 60 MQTPHILIVE DELVTRNTLK SIFEAEGYDV FEATDGAEMH QILSEYDINL VIMDINLPGK 70 80 90 100 110 120 NGLLLARELR EQANVALMFL TGRDNEVDKI LGLEIGADDY ITKPFNPREL TIRARNLLSR 130 140 150 160 170 180 TMNLGTVSEE RRSVESYKFN GWELDINSRS LIGPDGEQYK LPRSEFRAML HFCENPGKIQ 190 200 210 220 230 SRAELLKKMT GRELKPHDRT VDVTIRRIRK HFESTPDTPE IIATIHGEGY RFCGDLED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of the dye gene of Escherichia coli reveals amino acid homology between the dye and OmpR proteins." Drury L.S., Buxton R.S. J. Biol. Chem. 260:4236-4242(1985) [PubMed: 2984198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	Park S.J., Gunsalus R.P. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23. Strain: K12.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
[7]	"Purification of ArcA and analysis of its specific interaction with the pfl promoter-regulatory region." Drapal N., Sawers G. Mol. Microbiol. 16:597-607(1995) [PubMed: 7565118] [Abstract] Cited for: PROTEIN SEQUENCE OF 40-45 AND 123-128, CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M10044 Genomic DNA. Translation: AAA23718.1.
U14003 Genomic DNA. Translation: AAA97297.1.
U00096 Genomic DNA. Translation: AAC77354.1.
AP009048 Genomic DNA. Translation: BAE78390.1.
L34010 Genomic DNA. Translation: AAA23476.1.

PIR

JYECR. A03561.

RefSeq

AP_004889.1.
NP_418818.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XHE	X-ray	2.50	A/B	1-122	[»]
1XHF	X-ray	2.15	A/B	1-122	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A9Q1. 4 interactions.

2-D gel databases

SWISS-2DPAGE

P0A9Q1.

Genome annotation databases

GeneID

948874.

GenomeReviews

Gene locus JW4364 in contig AP009048_GR.
Gene locus b4401 in contig U00096_GR.

KEGG

ecj:JW4364.
eco:b4401.

Organism-specific databases

EchoBASE

EB0059.

EcoGene

EG10061. arcA.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A9Q1.

OMA

P0A9Q1. ESYRFNG.

Enzyme and pathway databases

BioCyc

EcoCyc:ARCA-MON.

Family and domain databases

InterPro

IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]

Pfam

PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]

ProDom

PD000039. Response_reg. 1 hit.
PD000329. Trans_reg_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00448. REC. 1 hit.
[Graphical view]

PROSITE

PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ARCA_ECOLI

Accession

Primary (citable) accession number: P0A9Q1
Secondary accession number(s): P03026, Q2M5R6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)