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P0A9Q1

- ARCA_ECOLI

UniProt

P0A9Q1 - ARCA_ECOLI

Protein

Aerobic respiration control protein ArcA

Gene

arcA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Member of the two-component regulatory system ArcB/ArcA. Represses a wide variety of aerobic enzymes under anaerobic conditions. Controls the resistance of E.coli to dyes; required for expression of the alkaline phosphatase and sex factor F genes; It also may be involved in the osmoregulation of envelope proteins. When activated by ArcB, it negatively regulates the expression of genes of aerobic function. Activates the transcription of the plfB operon by binding to its promoter.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. identical protein binding Source: IntAct
    3. phosphorelay response regulator activity Source: EcoCyc
    4. protein binding Source: IntAct
    5. sequence-specific DNA binding transcription factor activity Source: EcoCyc

    GO - Biological processi

    1. intracellular signal transduction Source: GOC
    2. negative regulation of transcription, DNA-templated Source: EcoCyc
    3. phosphorelay signal transduction system Source: EcoCyc
    4. positive regulation of transcription, DNA-templated Source: EcoCyc
    5. regulation of transcription, DNA-templated Source: EcoCyc
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:ARCA-MONOMER.
    ECOL316407:JW4364-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aerobic respiration control protein ArcA
    Alternative name(s):
    Dye resistance protein
    Gene namesi
    Name:arcA
    Synonyms:cpxC, dye, fexA, msp, seg, sfrA
    Ordered Locus Names:b4401, JW4364
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10061. arcA.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238Aerobic respiration control protein ArcAPRO_0000081008Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei54 – 5414-aspartylphosphatePROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylated by ArcB.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP0A9Q1.
    PRIDEiP0A9Q1.

    2D gel databases

    SWISS-2DPAGEP0A9Q1.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A9Q1.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-1119939,EBI-1119939
    mexBP520022EBI-1119939,EBI-6400435From a different organism.

    Protein-protein interaction databases

    DIPiDIP-36035N.
    IntActiP0A9Q1. 7 interactions.
    STRINGi511145.b4401.

    Structurei

    Secondary structure

    1
    238
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 95
    Helixi13 – 2412
    Turni25 – 273
    Beta strandi29 – 357
    Helixi36 – 4510
    Beta strandi49 – 535
    Beta strandi58 – 603
    Helixi62 – 7211
    Beta strandi76 – 827
    Helixi86 – 9510
    Beta strandi98 – 1047
    Helixi107 – 12115

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1XHEX-ray2.50A/B1-123[»]
    1XHFX-ray2.15A/B1-123[»]
    ProteinModelPortaliP0A9Q1.
    SMRiP0A9Q1. Positions 1-234.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A9Q1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 118118Response regulatoryPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0745.
    HOGENOMiHOG000034819.
    KOiK07773.
    OMAiDAYRFNG.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiP0A9Q1.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A9Q1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQTPHILIVE DELVTRNTLK SIFEAEGYDV FEATDGAEMH QILSEYDINL    50
    VIMDINLPGK NGLLLARELR EQANVALMFL TGRDNEVDKI LGLEIGADDY 100
    ITKPFNPREL TIRARNLLSR TMNLGTVSEE RRSVESYKFN GWELDINSRS 150
    LIGPDGEQYK LPRSEFRAML HFCENPGKIQ SRAELLKKMT GRELKPHDRT 200
    VDVTIRRIRK HFESTPDTPE IIATIHGEGY RFCGDLED 238
    Length:238
    Mass (Da):27,292
    Last modified:July 21, 1986 - v1
    Checksum:i9316CF4DE8EABDE8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10044 Genomic DNA. Translation: AAA23718.1.
    U14003 Genomic DNA. Translation: AAA97297.1.
    U00096 Genomic DNA. Translation: AAC77354.1.
    AP009048 Genomic DNA. Translation: BAE78390.1.
    L34010 Genomic DNA. Translation: AAA23476.1.
    PIRiA03561. JYECR.
    RefSeqiNP_418818.1. NC_000913.3.
    YP_492531.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77354; AAC77354; b4401.
    BAE78390; BAE78390; BAE78390.
    GeneIDi12930392.
    948874.
    KEGGiecj:Y75_p4285.
    eco:b4401.
    PATRICi32124420. VBIEscCol129921_4550.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10044 Genomic DNA. Translation: AAA23718.1 .
    U14003 Genomic DNA. Translation: AAA97297.1 .
    U00096 Genomic DNA. Translation: AAC77354.1 .
    AP009048 Genomic DNA. Translation: BAE78390.1 .
    L34010 Genomic DNA. Translation: AAA23476.1 .
    PIRi A03561. JYECR.
    RefSeqi NP_418818.1. NC_000913.3.
    YP_492531.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1XHE X-ray 2.50 A/B 1-123 [» ]
    1XHF X-ray 2.15 A/B 1-123 [» ]
    ProteinModelPortali P0A9Q1.
    SMRi P0A9Q1. Positions 1-234.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-36035N.
    IntActi P0A9Q1. 7 interactions.
    STRINGi 511145.b4401.

    2D gel databases

    SWISS-2DPAGE P0A9Q1.

    Proteomic databases

    PaxDbi P0A9Q1.
    PRIDEi P0A9Q1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77354 ; AAC77354 ; b4401 .
    BAE78390 ; BAE78390 ; BAE78390 .
    GeneIDi 12930392.
    948874.
    KEGGi ecj:Y75_p4285.
    eco:b4401.
    PATRICi 32124420. VBIEscCol129921_4550.

    Organism-specific databases

    EchoBASEi EB0059.
    EcoGenei EG10061. arcA.

    Phylogenomic databases

    eggNOGi COG0745.
    HOGENOMi HOG000034819.
    KOi K07773.
    OMAi DAYRFNG.
    OrthoDBi EOG6G4VQG.
    PhylomeDBi P0A9Q1.

    Enzyme and pathway databases

    BioCyci EcoCyc:ARCA-MONOMER.
    ECOL316407:JW4364-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A9Q1.
    PROi P0A9Q1.

    Gene expression databases

    Genevestigatori P0A9Q1.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequence analysis of the dye gene of Escherichia coli reveals amino acid homology between the dye and OmpR proteins."
      Drury L.S., Buxton R.S.
      J. Biol. Chem. 260:4236-4242(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. Park S.J., Gunsalus R.P.
      Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
      Strain: K12.
    6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-12.
      Strain: K12 / EMG2.
    7. "Purification of ArcA and analysis of its specific interaction with the pfl promoter-regulatory region."
      Drapal N., Sawers G.
      Mol. Microbiol. 16:597-607(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 40-45 AND 123-128, CHARACTERIZATION.

    Entry informationi

    Entry nameiARCA_ECOLI
    AccessioniPrimary (citable) accession number: P0A9Q1
    Secondary accession number(s): P03026, Q2M5R6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3