ID   IDNO_ECOLI              Reviewed;         254 AA.
AC   P0A9P9; P39345; Q2M643;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 36.
DE   RecName: Full=Gluconate 5-dehydrogenase;
DE            EC=1.1.1.69;
DE   AltName: Full=5-keto-D-gluconate 5-reductase;
GN   Name=idnO; Synonyms=yjgU; OrderedLocusNames=b4266, JW4223;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=95334362; PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA   Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.,
RA   Blattner F.R.;
RT   "Analysis of the Escherichia coli genome VI: DNA sequence of the
RT   region from 92.8 through 100 minutes.";
RL   Nucleic Acids Res. 23:2105-2119(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION.
RX   MEDLINE=98324983; PubMed=9658018;
RA   Bausch C., Peekhaus N., Utz C., Blais T., Murray E., Lowary T.,
RA   Conway T.;
RT   "Sequence analysis of the GntII (subsidiary) system for gluconate
RT   metabolism reveals a novel pathway for L-idonic acid catabolism in
RT   Escherichia coli.";
RL   J. Bacteriol. 180:3704-3710(1998).
CC   -!- FUNCTION: Catalyzes a reversible reduction of 5-ketoglutanate to
CC       form D-gluconate. Dependent on NADP, almost inactive with NAD.
CC   -!- CATALYTIC ACTIVITY: D-gluconate + NAD(P)(+) = 5-dehydro-D-
CC       gluconate + NAD(P)H.
CC   -!- PATHWAY: Carbohydrate acid metabolism; L-idonic acid degradation.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; U14003; AAA97163.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC77223.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE78263.1; -; Genomic_DNA.
DR   PIR; S56492; S56492.
DR   RefSeq; AP_004762.1; -.
DR   RefSeq; NP_418687.1; -.
DR   HSSP; P25529; 1AHH.
DR   GeneID; 947109; -.
DR   GenomeReviews; AP009048_GR; JW4223.
DR   GenomeReviews; U00096_GR; b4266.
DR   KEGG; ecj:JW4223; -.
DR   KEGG; eco:b4266; -.
DR   EchoBASE; EB2429; -.
DR   EcoGene; EG12540; idnO.
DR   HOGENOM; P0A9P9; -.
DR   OMA; P0A9P9; RYMIKRQ.
DR   BioCyc; EcoCyc:GLUCONREDUCT-MON; -.
DR   BioCyc; MetaCyc:GLUCONREDUCT-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0008874; F:gluconate 5-dehydrogenase activity; IEA:EC.
DR   GO; GO:0019521; P:D-gluconate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Gluconate utilization; NADP;
KW   Oxidoreductase.
FT   CHAIN         1    254       Gluconate 5-dehydrogenase.
FT                                /FTId=PRO_0000054702.
FT   NP_BIND      13     37       NADP (By similarity).
FT   ACT_SITE    158    158       Proton acceptor (By similarity).
FT   BINDING     145    145       Substrate (By similarity).
SQ   SEQUENCE   254 AA;  27563 MW;  C5AA4A044CEC1E6E CRC64;
     MNDLFSLAGK NILITGSAQG IGFLLATGLG KYGAQIIIND ITAERAELAV EKLHQEGIQA
     VAAPFNVTHK HEIDAAVEHI EKDIGPIDVL VNNAGIQRRH PFTEFPEQEW NDVIAVNQTA
     VFLVSQAVTR HMVERKAGKV INICSMQSEL GRDTITPYAA SKGAVKMLTR GMCVELARHN
     IQVNGIAPGY FKTEMTKALV EDEAFTAWLC KRTPAARWGD PQELIGAAVF LSSKASDFVN
     GHLLFVDGGM LVAV
//