P0A9P9 (IDNO_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 68.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Gluconate 5-dehydrogenase EC=1.1.1.69 Alternative name(s): 5-keto-D-gluconate 5-reductase | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Reference proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › ![]() |
Protein attributes
| Sequence length | 254 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Catalyzes a reversible reduction of 5-ketoglutanate to form D-gluconate. Dependent on NADP, almost inactive with NAD. Ref.4 |
| Catalytic activity | D-gluconate + NAD(P)+ = 5-dehydro-D-gluconate + NAD(P)H. |
| Pathway | |
| Subcellular location | Cytoplasm Probable. |
| Sequence similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Gluconate utilization |
| Cellular component | Cytoplasm |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | D-gluconate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW L-idonate catabolic processInferred from expression pattern Ref.4. Source: EcoCyc |
| Cellular_component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | gluconate 5-dehydrogenase activity Inferred from direct assay PubMed 10427017Ref.4. Source: EcoCyc nucleotide bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [2] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [4] | "Sequence analysis of the GntII (subsidiary) system for gluconate metabolism reveals a novel pathway for L-idonic acid catabolism in Escherichia coli." Bausch C., Peekhaus N., Utz C., Blais T., Murray E., Lowary T., Conway T. J. Bacteriol. 180:3704-3710(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U14003 Genomic DNA. Translation: AAA97163.1. U00096 Genomic DNA. Translation: AAC77223.1. AP009048 Genomic DNA. Translation: BAE78263.1. |
| PIR | S56492. |
| RefSeq | NP_418687.1. NC_000913.2. YP_492404.1. NC_007779.1. |
3D structure databases | |
| ProteinModelPortal | P0A9P9. |
| SMR | P0A9P9. Positions 3-254. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P0A9P9. 5 interactions. |
| MINT | MINT-1231433. |
| STRING | 511145.b4266. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | AAC77223; AAC77223; b4266. BAE78263; BAE78263; BAE78263. |
| GeneID | 12933736. 947109. |
| KEGG | ecj:Y75_p4149. eco:b4266. |
| PATRIC | 32124105. VBIEscCol129921_4397. |
Organism-specific databases | |
| EchoBASE | EB2429. |
| EcoGene | EG12540. idnO. |
Phylogenomic databases | |
| eggNOG | COG1028. |
| KO | K00046. |
| OMA | VNICSMQ. |
| ProtClustDB | PRK08085. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:GLUCONREDUCT-MONOMER. ECOL316407:JW4223-MONOMER. MetaCyc:GLUCONREDUCT-MONOMER. |
| UniPathway | UPA00793. |
Gene expression databases | |
| Genevestigator | P0A9P9. |
Family and domain databases | |
| Gene3D | 3.40.50.720. 1 hit. |
| InterPro | IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view] |
| Pfam | PF00106. adh_short. 1 hit. [Graphical view] |
| PRINTS | PR00081. GDHRDH. PR00080. SDRFAMILY. |
| PROSITE | PS00061. ADH_SHORT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | IDNO_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0A9P9 Secondary accession number(s): P39345, Q2M643 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
