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Protein

5-keto-D-gluconate 5-reductase

Gene

idnO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of 5-keto-D-gluconate to D-gluconate, using either NADH or NADPH. Is likely involved in an L-idonate degradation pathway that allows E.coli to utilize L-idonate as the sole carbon and energy source. Is also able to catalyze the reverse reaction in vitro, but the D-gluconate oxidation by the enzyme can only proceed with NAD.1 Publication

Catalytic activityi

D-gluconate + NAD(P)+ = 5-dehydro-D-gluconate + NAD(P)H.1 Publication

Kineticsi

  1. KM=0.5 mM for 5-keto-D-gluconate1 Publication
  2. KM=2 mM for D-gluconate1 Publication

    Pathwayi: L-idonate degradation

    This protein is involved in the pathway L-idonate degradation, which is part of Carbohydrate acid metabolism.1 Publication
    View all proteins of this organism that are known to be involved in the pathway L-idonate degradation and in Carbohydrate acid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei145SubstrateBy similarity1
    Active sitei158Proton acceptorPROSITE-ProRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi13 – 37NADPBy similarityAdd BLAST25

    GO - Molecular functioni

    • gluconate 5-dehydrogenase activity Source: EcoCyc

    GO - Biological processi

    • L-idonate catabolic process Source: EcoCyc

    Keywordsi

    Molecular functionOxidoreductase
    Biological processCarbohydrate metabolism
    LigandNAD, NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:GLUCONREDUCT-MONOMER.
    MetaCyc:GLUCONREDUCT-MONOMER.
    UniPathwayiUPA00793.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-keto-D-gluconate 5-reductase1 Publication (EC:1.1.1.691 Publication)
    Gene namesi
    Name:idnO1 Publication
    Synonyms:yjgU
    Ordered Locus Names:b4266, JW4223
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12540. idnO.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000547021 – 2545-keto-D-gluconate 5-reductaseAdd BLAST254

    Proteomic databases

    PaxDbiP0A9P9.
    PRIDEiP0A9P9.

    Expressioni

    Inductioni

    Is up-regulated by L-idonate (15-fold) and by 5-ketogluconate (80-fold). Seems to be catabolite repressed. Is probably under the control of the positive regulator IdnR. Is part of the idnDOTR operon.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi4262730. 6 interactors.
    IntActiP0A9P9. 7 interactors.
    MINTiMINT-1231433.
    STRINGi316385.ECDH10B_4459.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A9P9.
    SMRiP0A9P9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105CHR. Bacteria.
    ENOG410XNW1. LUCA.
    InParanoidiP0A9P9.
    KOiK00046.
    PhylomeDBiP0A9P9.

    Family and domain databases

    InterProiView protein in InterPro
    IPR036291. NAD(P)-bd_dom_sf.
    IPR020904. Sc_DH/Rdtase_CS.
    IPR002347. SDR_fam.
    PfamiView protein in Pfam
    PF00106. adh_short. 1 hit.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiView protein in PROSITE
    PS00061. ADH_SHORT. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A9P9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNDLFSLAGK NILITGSAQG IGFLLATGLG KYGAQIIIND ITAERAELAV
    60 70 80 90 100
    EKLHQEGIQA VAAPFNVTHK HEIDAAVEHI EKDIGPIDVL VNNAGIQRRH
    110 120 130 140 150
    PFTEFPEQEW NDVIAVNQTA VFLVSQAVTR HMVERKAGKV INICSMQSEL
    160 170 180 190 200
    GRDTITPYAA SKGAVKMLTR GMCVELARHN IQVNGIAPGY FKTEMTKALV
    210 220 230 240 250
    EDEAFTAWLC KRTPAARWGD PQELIGAAVF LSSKASDFVN GHLLFVDGGM

    LVAV
    Length:254
    Mass (Da):27,563
    Last modified:July 19, 2005 - v1
    Checksum:iC5AA4A044CEC1E6E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U14003 Genomic DNA. Translation: AAA97163.1.
    U00096 Genomic DNA. Translation: AAC77223.1.
    AP009048 Genomic DNA. Translation: BAE78263.1.
    PIRiS56492.
    RefSeqiNP_418687.1. NC_000913.3.
    WP_000998695.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77223; AAC77223; b4266.
    BAE78263; BAE78263; BAE78263.
    GeneIDi947109.
    KEGGiecj:JW4223.
    eco:b4266.
    PATRICifig|1411691.4.peg.2437.

    Similar proteinsi

    Entry informationi

    Entry nameiIDNO_ECOLI
    AccessioniPrimary (citable) accession number: P0A9P9
    Secondary accession number(s): P39345, Q2M643
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: October 25, 2017
    This is version 96 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families