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Protein

Gluconate 5-dehydrogenase

Gene

idnO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes a reversible reduction of 5-ketoglutanate to form D-gluconate. Dependent on NADP, almost inactive with NAD.1 Publication

Catalytic activityi

D-gluconate + NAD(P)+ = 5-dehydro-D-gluconate + NAD(P)H.

Pathwayi: L-idonate degradation

This protein is involved in the pathway L-idonate degradation, which is part of Carbohydrate acid metabolism.
View all proteins of this organism that are known to be involved in the pathway L-idonate degradation and in Carbohydrate acid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei145 – 1451SubstrateBy similarity
Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 3725NADPBy similarityAdd
BLAST

GO - Molecular functioni

  • gluconate 5-dehydrogenase activity Source: EcoCyc

GO - Biological processi

  • D-gluconate metabolic process Source: UniProtKB-KW
  • L-idonate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Gluconate utilization

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:GLUCONREDUCT-MONOMER.
ECOL316407:JW4223-MONOMER.
MetaCyc:GLUCONREDUCT-MONOMER.
RETL1328306-WGS:GSTH-5128-MONOMER.
RETL1328306-WGS:GSTH-6754-MONOMER.
UniPathwayiUPA00793.

Names & Taxonomyi

Protein namesi
Recommended name:
Gluconate 5-dehydrogenase (EC:1.1.1.69)
Alternative name(s):
5-keto-D-gluconate 5-reductase
Gene namesi
Name:idnO
Synonyms:yjgU
Ordered Locus Names:b4266, JW4223
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12540. idnO.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Gluconate 5-dehydrogenasePRO_0000054702Add
BLAST

Proteomic databases

PaxDbiP0A9P9.

Interactioni

Protein-protein interaction databases

BioGridi4262730. 6 interactions.
IntActiP0A9P9. 7 interactions.
MINTiMINT-1231433.
STRINGi511145.b4266.

Structurei

3D structure databases

ProteinModelPortaliP0A9P9.
SMRiP0A9P9. Positions 3-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
InParanoidiP0A9P9.
KOiK00046.
OMAiRYMIKRQ.
OrthoDBiEOG6N3CR8.
PhylomeDBiP0A9P9.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9P9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDLFSLAGK NILITGSAQG IGFLLATGLG KYGAQIIIND ITAERAELAV
60 70 80 90 100
EKLHQEGIQA VAAPFNVTHK HEIDAAVEHI EKDIGPIDVL VNNAGIQRRH
110 120 130 140 150
PFTEFPEQEW NDVIAVNQTA VFLVSQAVTR HMVERKAGKV INICSMQSEL
160 170 180 190 200
GRDTITPYAA SKGAVKMLTR GMCVELARHN IQVNGIAPGY FKTEMTKALV
210 220 230 240 250
EDEAFTAWLC KRTPAARWGD PQELIGAAVF LSSKASDFVN GHLLFVDGGM

LVAV
Length:254
Mass (Da):27,563
Last modified:July 19, 2005 - v1
Checksum:iC5AA4A044CEC1E6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97163.1.
U00096 Genomic DNA. Translation: AAC77223.1.
AP009048 Genomic DNA. Translation: BAE78263.1.
PIRiS56492.
RefSeqiNP_418687.1. NC_000913.3.
WP_000998695.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77223; AAC77223; b4266.
BAE78263; BAE78263; BAE78263.
GeneIDi947109.
KEGGiecj:JW4223.
eco:b4266.
PATRICi32124105. VBIEscCol129921_4397.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97163.1.
U00096 Genomic DNA. Translation: AAC77223.1.
AP009048 Genomic DNA. Translation: BAE78263.1.
PIRiS56492.
RefSeqiNP_418687.1. NC_000913.3.
WP_000998695.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9P9.
SMRiP0A9P9. Positions 3-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262730. 6 interactions.
IntActiP0A9P9. 7 interactions.
MINTiMINT-1231433.
STRINGi511145.b4266.

Proteomic databases

PaxDbiP0A9P9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77223; AAC77223; b4266.
BAE78263; BAE78263; BAE78263.
GeneIDi947109.
KEGGiecj:JW4223.
eco:b4266.
PATRICi32124105. VBIEscCol129921_4397.

Organism-specific databases

EchoBASEiEB2429.
EcoGeneiEG12540. idnO.

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
InParanoidiP0A9P9.
KOiK00046.
OMAiRYMIKRQ.
OrthoDBiEOG6N3CR8.
PhylomeDBiP0A9P9.

Enzyme and pathway databases

UniPathwayiUPA00793.
BioCyciEcoCyc:GLUCONREDUCT-MONOMER.
ECOL316407:JW4223-MONOMER.
MetaCyc:GLUCONREDUCT-MONOMER.
RETL1328306-WGS:GSTH-5128-MONOMER.
RETL1328306-WGS:GSTH-6754-MONOMER.

Miscellaneous databases

PROiP0A9P9.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Sequence analysis of the GntII (subsidiary) system for gluconate metabolism reveals a novel pathway for L-idonic acid catabolism in Escherichia coli."
    Bausch C., Peekhaus N., Utz C., Blais T., Murray E., Lowary T., Conway T.
    J. Bacteriol. 180:3704-3710(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiIDNO_ECOLI
AccessioniPrimary (citable) accession number: P0A9P9
Secondary accession number(s): P39345, Q2M643
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: April 13, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.