P0A9P7 (DEAD_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cold-shock DEAD box protein A
EC=3.6.4.13

Alternative name(s):
ATP-dependent RNA helicase DeaD

Gene names

Name:	deaD
Ordered Locus Names:	c3916

Organism

Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

199310 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has a helix-destabilizing activity. Plays a key role in optimal cell growth at low temperature and is required for normal cell division. Suppressor of a mutant defective in rpsB gene for ribosomal protein S2 By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DEAD box helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence AAN82357.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 629	628	Cold-shock DEAD box protein A		PRO_0000055103
Regions
	Domain	37 – 208	172	Helicase ATP-binding
	Domain	232 – 379	148	Helicase C-terminal
	Nucleotide binding	50 – 57	8	ATP By similarity
	Motif	6 – 34	29	Q motif
	Motif	156 – 159	4	DEAD box
	Compositional bias	568 – 629	62	Arg/Glu/Gly-rich

Sequences

	Sequence		Length	Mass (Da)
	P0A9P7 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9DB692D41C38D17C	FASTA	629	70,546
	10 20 30 40 50 60 MAEFETTFAD LGLKAPILEA LNDLGYEKPS PIQAECIPHL LNGRDVLGMA QTGSGKTAAF 70 80 90 100 110 120 SLPLLQNLDP ELKAPQILVL APTRELAVQV AEAMTDFSKH MRGVNVVALY GGQRYDVQLR 130 140 150 160 170 180 ALRQGPQIVV GTPGRLLDHL KRGTLDLSKL SGLVLDEADE MLRMGFIEDV ETIMAQIPEG 190 200 210 220 230 240 HQTALFSATM PEAIRRITRR FMKEPQEVRI QSSVTTRPDI SQSYWTVWGM RKNEALVRFL 250 260 270 280 290 300 EAEDFDAAII FVRTKNATLE VAEALERNGY NSAALNGDMN QALREQTLER LKDGRLDILI 310 320 330 340 350 360 ATDVAARGLD VERISLVVNY DIPMDSESYV HRIGRTGRAG RAGRALLFVE NRERRLLRNI 370 380 390 400 410 420 ERTMKLTIPE VELPNAELLG KRRLEKFAAK VQQQLESSDL DQYRALLSKI QPTAEGEELD 430 440 450 460 470 480 LETLAAALLK MAQGERTLIV PPDAPMRPKR EFRDRDDRGP RDRNDRGPRG DREDRPRRER 490 500 510 520 530 540 RDVGDMQLYR IEVGRDDGVE VRHIVGAIAN EGDISSRYIG NIKLFASHST IELPKGMPGE 550 560 570 580 590 600 VLQHFTRTRI LNKPMNMQLL GDAQPHTGGE RRGGGRGFGG ERREGGRNFS GERREGGRGD 610 620 GRRFSGERRE GRAPRRDDST GRRRFGGDA

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFT073 / ATCC 700928 / UPEC.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN82357.1. Different initiation.
RefSeq	NP_755783.2. NC_004431.1.
3D structure databases
ProteinModelPortal	P0A9P7.
SMR	P0A9P7. Positions 8-372.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1219382.
STRING	199310.c3916.
Proteomic databases
PRIDE	P0A9P7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN82357; AAN82357; c3916.
GeneID	1040245.
KEGG	ecc:c3916.
PATRIC	18285616. VBIEscCol75197_3690.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000268810.
KO	K05592.
OMA	ILFMTPR.
ProtClustDB	PRK11634.
Family and domain databases
InterPro	IPR021046. Cold-shock_DEAD_Abox_C. IPR005580. DbpA_RNA-bd. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF03880. DbpA. 1 hit. PF00270. DEAD. 1 hit. PF12343. DEADboxA. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DEAD_ECOL6

Accession

Primary (citable) accession number: P0A9P7
Secondary accession number(s): P23304, Q8FD90

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program