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Protein

Anaerobic ribonucleoside-triphosphate reductase-activating protein

Gene

nrdG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activation of anaerobic ribonucleoside-triphosphate reductase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.3 Publications

Cofactori

[4Fe-4S] cluster4 PublicationsNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi26Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Metal bindingi30Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Metal bindingi33Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Binding sitei74S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • [formate-C-acetyltransferase]-activating enzyme activity Source: InterPro
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • iron-sulfur cluster binding Source: EcoliWiki
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • nucleobase-containing small molecule interconversion Source: EcoliWiki

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:RNTRACTIV-MONOMER.
MetaCyc:RNTRACTIV-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic ribonucleoside-triphosphate reductase-activating proteinCurated (EC:1.97.1.-1 Publication)
Alternative name(s):
Class III anaerobic ribonucleotide reductase small componentCurated
Gene namesi
Name:nrdG
Synonyms:yjgE
Ordered Locus Names:b4237, JW4196
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12523. nrdG.

Subcellular locationi

GO - Cellular componenti

  • anaerobic ribonucleoside-triphosphate reductase complex Source: EcoliWiki

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19C → A: No change in activity. Does not affect iron and sulfide content. 1 Publication1
Mutagenesisi26C → A: Loss of activity. Decrease in iron and sulfide content. 1 Publication1
Mutagenesisi30C → A: Loss of activity. Decrease in iron and sulfide content. 1 Publication1
Mutagenesisi33C → A: Loss of activity. Decrease in iron and sulfide content. 1 Publication1
Mutagenesisi96C → A: No change in activity. Does not affect iron and sulfide content. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005351 – 154Anaerobic ribonucleoside-triphosphate reductase-activating proteinAdd BLAST154

Proteomic databases

PaxDbiP0A9N8.
PRIDEiP0A9N8.

Expressioni

Inductioni

Induced 2-fold by hydroxyurea.1 Publication

Interactioni

Subunit structurei

Monomer or homodimer (PubMed:7852304, PubMed:1460049, PubMed:8621608). Forms a tetramer composed of two NrdD and two NrdG subunits (PubMed:8621608).3 Publications

Protein-protein interaction databases

BioGridi4259317. 9 interactors.
DIPiDIP-48068N.
IntActiP0A9N8. 4 interactors.
STRINGi316385.ECDH10B_4432.

Structurei

3D structure databases

ProteinModelPortaliP0A9N8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 34S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108RDD. Bacteria.
COG0602. LUCA.
HOGENOMiHOG000222587.
InParanoidiP0A9N8.
KOiK04068.
PhylomeDBiP0A9N8.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR012837. NrdG.
IPR034457. Organic_radical-activating.
IPR001989. Radical_activat_CS.
PANTHERiPTHR30352:SF17. PTHR30352:SF17. 1 hit.
PIRSFiPIRSF000368. NrdG. 1 hit.
SFLDiSFLDF00299. anaerobic_ribonucleoside-triph. 1 hit.
SFLDG01066. organic_radical-activating_enz. 1 hit.
TIGRFAMsiTIGR02491. NrdG. 1 hit.
PROSITEiView protein in PROSITE
PS01087. RADICAL_ACTIVATING. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A9N8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYHQYYPVD IVNGPGTRCT LFVSGCVHEC PGCYNKSTWR VNSGQPFTKA
60 70 80 90 100
MEDQIINDLN DTRIKRQGIS LSGGDPLHPQ NVPDILKLVQ RIRAECPGKD
110 120 130 140 150
IWVWTGYKLD ELNAAQMQVV DLINVLVDGK FVQDLKDPSL IWRGSSNQVV

HHLR
Length:154
Mass (Da):17,446
Last modified:July 19, 2005 - v1
Checksum:i3BB3E9FACC782FD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46865 Genomic DNA. Translation: CAA86937.1.
U14003 Genomic DNA. Translation: AAA97134.1.
U00096 Genomic DNA. Translation: AAC77194.1.
AP009048 Genomic DNA. Translation: BAE78236.1.
PIRiA55692.
RefSeqiNP_418658.1. NC_000913.3.
WP_001106233.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77194; AAC77194; b4237.
BAE78236; BAE78236; BAE78236.
GeneIDi948757.
KEGGiecj:JW4196.
eco:b4237.
PATRICifig|1411691.4.peg.2465.

Similar proteinsi

Entry informationi

Entry nameiNRDG_ECOLI
AccessioniPrimary (citable) accession number: P0A9N8
Secondary accession number(s): P39329, Q2M670
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: August 30, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families