Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Anaerobic ribonucleoside-triphosphate reductase-activating protein

Gene

nrdG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activation of anaerobic ribonucleoside-triphosphate reductase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.3 Publications

Cofactori

[4Fe-4S] cluster4 PublicationsNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi26Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Metal bindingi30Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Metal bindingi33Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Binding sitei74S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • [formate-C-acetyltransferase]-activating enzyme activity Source: InterPro
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • iron-sulfur cluster binding Source: EcoliWiki
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • nucleobase-containing small molecule interconversion Source: EcoliWiki

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:RNTRACTIV-MONOMER
MetaCyc:RNTRACTIV-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic ribonucleoside-triphosphate reductase-activating proteinCurated (EC:1.97.1.-1 Publication)
Alternative name(s):
Class III anaerobic ribonucleotide reductase small componentCurated
Gene namesi
Name:nrdG
Synonyms:yjgE
Ordered Locus Names:b4237, JW4196
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12523 nrdG

Subcellular locationi

GO - Cellular componenti

  • anaerobic ribonucleoside-triphosphate reductase complex Source: EcoliWiki

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19C → A: No change in activity. Does not affect iron and sulfide content. 1 Publication1
Mutagenesisi26C → A: Loss of activity. Decrease in iron and sulfide content. 1 Publication1
Mutagenesisi30C → A: Loss of activity. Decrease in iron and sulfide content. 1 Publication1
Mutagenesisi33C → A: Loss of activity. Decrease in iron and sulfide content. 1 Publication1
Mutagenesisi96C → A: No change in activity. Does not affect iron and sulfide content. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005351 – 154Anaerobic ribonucleoside-triphosphate reductase-activating proteinAdd BLAST154

Proteomic databases

PaxDbiP0A9N8
PRIDEiP0A9N8

Expressioni

Inductioni

Induced 2-fold by hydroxyurea.1 Publication

Interactioni

Subunit structurei

Monomer or homodimer (PubMed:7852304, PubMed:1460049, PubMed:8621608). Forms a tetramer composed of two NrdD and two NrdG subunits (PubMed:8621608).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
nrdDP289033EBI-1135026,EBI-370011

Protein-protein interaction databases

BioGridi4259317, 14 interactors
DIPiDIP-48068N
IntActiP0A9N8, 4 interactors
STRINGi316385.ECDH10B_4432

Structurei

3D structure databases

ProteinModelPortaliP0A9N8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 34S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108RDD Bacteria
COG0602 LUCA
HOGENOMiHOG000222587
InParanoidiP0A9N8
KOiK04068
OMAiNQVIHYL
PhylomeDBiP0A9N8

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR012837 NrdG
IPR034457 Organic_radical-activating
IPR001989 Radical_activat_CS
IPR007197 rSAM
PANTHERiPTHR30352:SF2 PTHR30352:SF2, 1 hit
PIRSFiPIRSF000368 NrdG, 1 hit
SFLDiSFLDF00299 anaerobic_ribonucleoside-triph, 1 hit
SFLDG01066 organic_radical-activating_enz, 1 hit
SFLDS00029 Radical_SAM, 1 hit
TIGRFAMsiTIGR02491 NrdG, 1 hit
PROSITEiView protein in PROSITE
PS01087 RADICAL_ACTIVATING, 1 hit

Sequencei

Sequence statusi: Complete.

P0A9N8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYHQYYPVD IVNGPGTRCT LFVSGCVHEC PGCYNKSTWR VNSGQPFTKA
60 70 80 90 100
MEDQIINDLN DTRIKRQGIS LSGGDPLHPQ NVPDILKLVQ RIRAECPGKD
110 120 130 140 150
IWVWTGYKLD ELNAAQMQVV DLINVLVDGK FVQDLKDPSL IWRGSSNQVV

HHLR
Length:154
Mass (Da):17,446
Last modified:July 19, 2005 - v1
Checksum:i3BB3E9FACC782FD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46865 Genomic DNA Translation: CAA86937.1
U14003 Genomic DNA Translation: AAA97134.1
U00096 Genomic DNA Translation: AAC77194.1
AP009048 Genomic DNA Translation: BAE78236.1
PIRiA55692
RefSeqiNP_418658.1, NC_000913.3
WP_001106233.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77194; AAC77194; b4237
BAE78236; BAE78236; BAE78236
GeneIDi948757
KEGGiecj:JW4196
eco:b4237
PATRICifig|1411691.4.peg.2465

Similar proteinsi

Entry informationi

Entry nameiNRDG_ECOLI
AccessioniPrimary (citable) accession number: P0A9N8
Secondary accession number(s): P39329, Q2M670
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health