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Protein

Anaerobic ribonucleoside-triphosphate reductase-activating protein

Gene

nrdG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Activation of anaerobic ribonucleoside-triphosphate reductase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.

Cofactori

[4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi26Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi30Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi33Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Binding sitei74S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • [formate-C-acetyltransferase]-activating enzyme activity Source: InterPro
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • iron-sulfur cluster binding Source: EcoliWiki
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • nucleobase-containing small molecule interconversion Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:RNTRACTIV-MONOMER.
ECOL316407:JW4196-MONOMER.
MetaCyc:RNTRACTIV-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic ribonucleoside-triphosphate reductase-activating protein (EC:1.97.1.-)
Alternative name(s):
Class III anaerobic ribonucleotide reductase small component
Gene namesi
Name:nrdG
Synonyms:yjgE
Ordered Locus Names:b4237, JW4196
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12523. nrdG.

Subcellular locationi

GO - Cellular componenti

  • anaerobic ribonucleoside-triphosphate reductase complex Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005351 – 154Anaerobic ribonucleoside-triphosphate reductase-activating proteinAdd BLAST154

Proteomic databases

PaxDbiP0A9N8.
PRIDEiP0A9N8.

Expressioni

Inductioni

Induced 2-fold by hydroxyurea.1 Publication

Interactioni

Subunit structurei

Tetramer consisting of 2 alpha (NrdD) and 2 beta (NrdG) subunits.

Protein-protein interaction databases

BioGridi4259317. 9 interactors.
DIPiDIP-48068N.
IntActiP0A9N8. 4 interactors.
STRINGi511145.b4237.

Structurei

3D structure databases

ProteinModelPortaliP0A9N8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 34S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108RDD. Bacteria.
COG0602. LUCA.
HOGENOMiHOG000222587.
InParanoidiP0A9N8.
KOiK04068.
OMAiQRVRAEC.
PhylomeDBiP0A9N8.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012837. NrdG.
IPR001989. Radical_activat_CS.
[Graphical view]
PIRSFiPIRSF000368. NrdG. 1 hit.
TIGRFAMsiTIGR02491. NrdG. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9N8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYHQYYPVD IVNGPGTRCT LFVSGCVHEC PGCYNKSTWR VNSGQPFTKA
60 70 80 90 100
MEDQIINDLN DTRIKRQGIS LSGGDPLHPQ NVPDILKLVQ RIRAECPGKD
110 120 130 140 150
IWVWTGYKLD ELNAAQMQVV DLINVLVDGK FVQDLKDPSL IWRGSSNQVV

HHLR
Length:154
Mass (Da):17,446
Last modified:July 19, 2005 - v1
Checksum:i3BB3E9FACC782FD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46865 Genomic DNA. Translation: CAA86937.1.
U14003 Genomic DNA. Translation: AAA97134.1.
U00096 Genomic DNA. Translation: AAC77194.1.
AP009048 Genomic DNA. Translation: BAE78236.1.
PIRiA55692.
RefSeqiNP_418658.1. NC_000913.3.
WP_001106233.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77194; AAC77194; b4237.
BAE78236; BAE78236; BAE78236.
GeneIDi948757.
KEGGiecj:JW4196.
eco:b4237.
PATRICi32124047. VBIEscCol129921_4368.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46865 Genomic DNA. Translation: CAA86937.1.
U14003 Genomic DNA. Translation: AAA97134.1.
U00096 Genomic DNA. Translation: AAC77194.1.
AP009048 Genomic DNA. Translation: BAE78236.1.
PIRiA55692.
RefSeqiNP_418658.1. NC_000913.3.
WP_001106233.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9N8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259317. 9 interactors.
DIPiDIP-48068N.
IntActiP0A9N8. 4 interactors.
STRINGi511145.b4237.

Proteomic databases

PaxDbiP0A9N8.
PRIDEiP0A9N8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77194; AAC77194; b4237.
BAE78236; BAE78236; BAE78236.
GeneIDi948757.
KEGGiecj:JW4196.
eco:b4237.
PATRICi32124047. VBIEscCol129921_4368.

Organism-specific databases

EchoBASEiEB2414.
EcoGeneiEG12523. nrdG.

Phylogenomic databases

eggNOGiENOG4108RDD. Bacteria.
COG0602. LUCA.
HOGENOMiHOG000222587.
InParanoidiP0A9N8.
KOiK04068.
OMAiQRVRAEC.
PhylomeDBiP0A9N8.

Enzyme and pathway databases

BioCyciEcoCyc:RNTRACTIV-MONOMER.
ECOL316407:JW4196-MONOMER.
MetaCyc:RNTRACTIV-MONOMER.

Miscellaneous databases

PROiP0A9N8.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012837. NrdG.
IPR001989. Radical_activat_CS.
[Graphical view]
PIRSFiPIRSF000368. NrdG. 1 hit.
TIGRFAMsiTIGR02491. NrdG. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNRDG_ECOLI
AccessioniPrimary (citable) accession number: P0A9N8
Secondary accession number(s): P39329, Q2M670
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.