P0A9N8 (NRDG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anaerobic ribonucleoside-triphosphate reductase-activating protein
EC=1.97.1.-

Alternative name(s):
Class III anaerobic ribonucleotide reductase small component

Gene names

Name:	nrdG
Synonyms:	yjgE
Ordered Locus Names:	b4237, JW4196

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activation of anaerobic ribonucleoside-triphosphate reductase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Subunit structure	Tetramer consisting of 2 alpha (NrdD) and 2 beta (NrdG) subunits.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the organic radical-activating enzymes family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleobase-containing small molecule interconversion Inferred from mutant phenotype PubMed 8954104. Source: EcoliWiki
Cellular_component	anaerobic ribonucleoside-triphosphate reductase complex Inferred from mutant phenotype PubMed 8954104. Source: EcoliWiki
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from direct assay PubMed 10531327. Source: EcoCyc [formate-C-acetyltransferase]-activating enzyme activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	Anaerobic ribonucleoside-triphosphate reductase-activating protein		PRO_0000200535
Sites
	Metal binding	26	1	Iron-sulfur (4Fe-4S-S-AdoMet) Probable
	Metal binding	30	1	Iron-sulfur (4Fe-4S-S-AdoMet) Probable
	Metal binding	33	1	Iron-sulfur (4Fe-4S-S-AdoMet) Probable

Sequences

	Sequence		Length	Mass (Da)
	P0A9N8 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 3BB3E9FACC782FD5	FASTA	154	17,446
	10 20 30 40 50 60 MNYHQYYPVD IVNGPGTRCT LFVSGCVHEC PGCYNKSTWR VNSGQPFTKA MEDQIINDLN 70 80 90 100 110 120 DTRIKRQGIS LSGGDPLHPQ NVPDILKLVQ RIRAECPGKD IWVWTGYKLD ELNAAQMQVV 130 140 150 DLINVLVDGK FVQDLKDPSL IWRGSSNQVV HHLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Generation of the glycyl radical of the anaerobic Escherichia coli ribonucleotide reductase requires a specific activating enzyme." Sun X., Eliasson R., Pontis E., Andersson J., Buist G., Sjoeberg B.-M., Reichard P. J. Biol. Chem. 270:2443-2446(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The activating component of the anaerobic ribonucleotide reductase from Escherichia coli. An iron-sulfur center with only three cysteines." Tamarit J., Gerez C., Meier C., Mulliez E., Trautwein A., Fontecave M. J. Biol. Chem. 275:15669-15675(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IRON-SULFUR LIGANDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46865 Genomic DNA. Translation: CAA86937.1. U14003 Genomic DNA. Translation: AAA97134.1. U00096 Genomic DNA. Translation: AAC77194.1. AP009048 Genomic DNA. Translation: BAE78236.1.
PIR	A55692.
RefSeq	NP_418658.1. NC_000913.2. YP_492377.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A9N8.
SMR	P0A9N8. Positions 4-77.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48068N.
IntAct	P0A9N8. 2 interactions.
STRING	511145.b4237.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77194; AAC77194; b4237. BAE78236; BAE78236; BAE78236.
GeneID	12930323. 948757.
KEGG	ecj:Y75_p4122. eco:b4237.
PATRIC	32124047. VBIEscCol129921_4368.
Organism-specific databases
EchoBASE	EB2414.
EcoGene	EG12523. nrdG.
Phylogenomic databases
eggNOG	COG0602.
HOGENOM	HOG000222587.
KO	K04068.
OMA	QRVRAEC.
ProtClustDB	PRK11121.
Enzyme and pathway databases
BioCyc	EcoCyc:RNTRACTIV-MONOMER. ECOL316407:JW4196-MONOMER. MetaCyc:RNTRACTIV-MONOMER.
Gene expression databases
Genevestigator	P0A9N8.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR012837. NrdG. IPR001989. Radical_activat_CS. [Graphical view]
PIRSF	PIRSF000368. NrdG. 1 hit.
TIGRFAMs	TIGR02491. NrdG. 1 hit.
PROSITE	PS01087. RADICAL_ACTIVATING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NRDG_ECOLI

Accession

Primary (citable) accession number: P0A9N8
Secondary accession number(s): P39329, Q2M670

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program