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P0A9N7

- PFLA_SHIFL

UniProt

P0A9N7 - PFLA_SHIFL

Protein

Pyruvate formate-lyase 1-activating enzyme

Gene

pflA

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi30 – 301Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi34 – 341Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi37 – 371Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

    GO - Molecular functioni

    1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glucose metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
    Alternative name(s):
    Formate-C-acetyltransferase-activating enzyme 1
    PFL-activating enzyme 1
    Gene namesi
    Name:pflA
    Ordered Locus Names:SF0897, S0961
    OrganismiShigella flexneri
    Taxonomic identifieri623 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 246245Pyruvate formate-lyase 1-activating enzymePRO_0000200526Add
    BLAST

    Proteomic databases

    PaxDbiP0A9N7.

    Interactioni

    Protein-protein interaction databases

    STRINGi198214.SF0897.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A9N7.
    SMRiP0A9N7. Positions 2-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1180.
    HOGENOMiHOG000011458.
    KOiK04069.
    OrthoDBiEOG64FKHC.

    Family and domain databases

    InterProiIPR006638. Elp3/MiaB/NifB.
    IPR012838. PFL_activating.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02493. PFLA. 1 hit.
    PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A9N7-1 [UniParc]FASTAAdd to Basket

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    MSVIGRIHSF ESCGTVDGPG IRFITFFQGC LMRCLYCHNR DTWDTHGGKE    50
    VTVEDLMKEV VTYRHFMNAS GGGVTASGGE AILQAEFVRD WFRACKKEGI 100
    HTCLDTNGFV RRYDPVIDEL LEVTDLVMLD LKQMNDEIHQ NLVGVSNHRT 150
    LEFAKYLANK NVKVWIRYVV VPGWSDDDDS AHRLGEFTRD MGNVEKIELL 200
    PYHELGKHKW VAMGEEYKLD GVKPPKKETM ERVKGILEQY GHKVMF 246
    Length:246
    Mass (Da):28,204
    Last modified:January 23, 2007 - v2
    Checksum:i486E06A9CE97BF37
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN42527.2.
    AE014073 Genomic DNA. Translation: AAP16414.1.
    RefSeqiNP_706820.2. NC_004337.2.
    NP_836608.1. NC_004741.1.

    Genome annotation databases

    EnsemblBacteriaiAAN42527; AAN42527; SF0897.
    AAP16414; AAP16414; S0961.
    GeneIDi1023839.
    1077365.
    KEGGisfl:SF0897.
    sfx:S0961.
    PATRICi18703067. VBIShiFle31049_1042.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN42527.2 .
    AE014073 Genomic DNA. Translation: AAP16414.1 .
    RefSeqi NP_706820.2. NC_004337.2.
    NP_836608.1. NC_004741.1.

    3D structure databases

    ProteinModelPortali P0A9N7.
    SMRi P0A9N7. Positions 2-246.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198214.SF0897.

    Proteomic databases

    PaxDbi P0A9N7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN42527 ; AAN42527 ; SF0897 .
    AAP16414 ; AAP16414 ; S0961 .
    GeneIDi 1023839.
    1077365.
    KEGGi sfl:SF0897.
    sfx:S0961.
    PATRICi 18703067. VBIShiFle31049_1042.

    Phylogenomic databases

    eggNOGi COG1180.
    HOGENOMi HOG000011458.
    KOi K04069.
    OrthoDBi EOG64FKHC.

    Family and domain databases

    InterProi IPR006638. Elp3/MiaB/NifB.
    IPR012838. PFL_activating.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02493. PFLA. 1 hit.
    PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
      Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
      , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
      Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 301 / Serotype 2a.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700930 / 2457T / Serotype 2a.

    Entry informationi

    Entry nameiPFLA_SHIFL
    AccessioniPrimary (citable) accession number: P0A9N7
    Secondary accession number(s): P09374
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 60 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3