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P0A9N7

- PFLA_SHIFL

UniProt

P0A9N7 - PFLA_SHIFL

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Protein
Pyruvate formate-lyase 1-activating enzyme
Gene
pflA, SF0897, S0961
Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi34 – 341Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi37 – 371Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 1
PFL-activating enzyme 1
Gene namesi
Name:pflA
Ordered Locus Names:SF0897, S0961
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 246245Pyruvate formate-lyase 1-activating enzyme
PRO_0000200526Add
BLAST

Proteomic databases

PaxDbiP0A9N7.

Interactioni

Protein-protein interaction databases

STRINGi198214.SF0897.

Structurei

3D structure databases

ProteinModelPortaliP0A9N7.
SMRiP0A9N7. Positions 2-246.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011458.
KOiK04069.
OrthoDBiEOG64FKHC.

Family and domain databases

InterProiIPR006638. Elp3/MiaB/NifB.
IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9N7-1 [UniParc]FASTAAdd to Basket

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MSVIGRIHSF ESCGTVDGPG IRFITFFQGC LMRCLYCHNR DTWDTHGGKE    50
VTVEDLMKEV VTYRHFMNAS GGGVTASGGE AILQAEFVRD WFRACKKEGI 100
HTCLDTNGFV RRYDPVIDEL LEVTDLVMLD LKQMNDEIHQ NLVGVSNHRT 150
LEFAKYLANK NVKVWIRYVV VPGWSDDDDS AHRLGEFTRD MGNVEKIELL 200
PYHELGKHKW VAMGEEYKLD GVKPPKKETM ERVKGILEQY GHKVMF 246
Length:246
Mass (Da):28,204
Last modified:January 23, 2007 - v2
Checksum:i486E06A9CE97BF37
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN42527.2.
AE014073 Genomic DNA. Translation: AAP16414.1.
RefSeqiNP_706820.2. NC_004337.2.
NP_836608.1. NC_004741.1.

Genome annotation databases

EnsemblBacteriaiAAN42527; AAN42527; SF0897.
AAP16414; AAP16414; S0961.
GeneIDi1023839.
1077365.
KEGGisfl:SF0897.
sfx:S0961.
PATRICi18703067. VBIShiFle31049_1042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN42527.2 .
AE014073 Genomic DNA. Translation: AAP16414.1 .
RefSeqi NP_706820.2. NC_004337.2.
NP_836608.1. NC_004741.1.

3D structure databases

ProteinModelPortali P0A9N7.
SMRi P0A9N7. Positions 2-246.
ModBasei Search...

Protein-protein interaction databases

STRINGi 198214.SF0897.

Proteomic databases

PaxDbi P0A9N7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN42527 ; AAN42527 ; SF0897 .
AAP16414 ; AAP16414 ; S0961 .
GeneIDi 1023839.
1077365.
KEGGi sfl:SF0897.
sfx:S0961.
PATRICi 18703067. VBIShiFle31049_1042.

Phylogenomic databases

eggNOGi COG1180.
HOGENOMi HOG000011458.
KOi K04069.
OrthoDBi EOG64FKHC.

Family and domain databases

InterProi IPR006638. Elp3/MiaB/NifB.
IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02493. PFLA. 1 hit.
PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiPFLA_SHIFL
AccessioniPrimary (citable) accession number: P0A9N7
Secondary accession number(s): P09374
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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