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Protein

Pyruvate formate-lyase 1-activating enzyme

Gene

pflA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi34Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi37Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Binding sitei79S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei203S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 1
PFL-activating enzyme 1
Gene namesi
Name:pflA
Ordered Locus Names:Z1246, ECs0985
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002005232 – 246Pyruvate formate-lyase 1-activating enzymeAdd BLAST245

Interactioni

Protein-protein interaction databases

STRINGi155864.Z1246

Structurei

3D structure databases

ProteinModelPortaliP0A9N6
SMRiP0A9N6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 38S-adenosyl-L-methionine bindingBy similarity3
Regioni130 – 132S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F1A Bacteria
COG1180 LUCA
HOGENOMiHOG000011458
KOiK04069
OMAiIGVPNKR

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR034457 Organic_radical-activating
IPR012839 Organic_radical_activase
IPR012838 PFL1_activating
IPR034465 Pyruvate_for-lyase_activase
IPR001989 Radical_activat_CS
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF000371 PFL_act_enz, 1 hit
SFLDiSFLDF00278 pyruvate_formate-lyase_activas, 1 hit
SFLDG01066 organic_radical-activating_enz, 1 hit
SFLDS00029 Radical_SAM, 1 hit
TIGRFAMsiTIGR02493 PFLA, 1 hit
PROSITEiView protein in PROSITE
PS01087 RADICAL_ACTIVATING, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9N6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVIGRIHSF ESCGTVDGPG IRFITFFQGC LMRCLYCHNR DTWDTHGGKE
60 70 80 90 100
VTVEDLMKEV VTYRHFMNAS GGGVTASGGE AILQAEFVRD WFRACKKEGI
110 120 130 140 150
HTCLDTNGFV RRYDPVIDEL LEVTDLVMLD LKQMNDEIHQ NLVGVSNHRT
160 170 180 190 200
LEFAKYLANK NVKVWIRYVV VPGWSDDDDS AHRLGEFTRD MGNVEKIELL
210 220 230 240
PYHELGKHKW VAMGEEYKLD GVKPPKKETM ERVKGILEQY GHKVMF
Length:246
Mass (Da):28,204
Last modified:January 23, 2007 - v2
Checksum:i486E06A9CE97BF37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG55387.1
BA000007 Genomic DNA Translation: BAB34408.1
PIRiA99752
G85615
RefSeqiNP_309012.1, NC_002695.1
WP_000111043.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG55387; AAG55387; Z1246
BAB34408; BAB34408; BAB34408
GeneIDi917728
KEGGiece:Z1246
ecs:ECs0985
PATRICifig|386585.9.peg.1104

Similar proteinsi

Entry informationi

Entry nameiPFLA_ECO57
AccessioniPrimary (citable) accession number: P0A9N6
Secondary accession number(s): P09374
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 86 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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