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P0A9N6

- PFLA_ECO57

UniProt

P0A9N6 - PFLA_ECO57

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Protein
Pyruvate formate-lyase 1-activating enzyme
Gene
pflA, Z1246, ECs0985
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi34 – 341Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi37 – 371Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOL386585:GJFA-983-MONOMER.
ECOO157:PFLA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 1
PFL-activating enzyme 1
Gene namesi
Name:pflA
Ordered Locus Names:Z1246, ECs0985
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 246245Pyruvate formate-lyase 1-activating enzyme
PRO_0000200523Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi155864.Z1246.

Structurei

3D structure databases

ProteinModelPortaliP0A9N6.
SMRiP0A9N6. Positions 2-246.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011458.
KOiK04069.
OMAiQNLIGVP.
OrthoDBiEOG64FKHC.

Family and domain databases

InterProiIPR006638. Elp3/MiaB/NifB.
IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9N6-1 [UniParc]FASTAAdd to Basket

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MSVIGRIHSF ESCGTVDGPG IRFITFFQGC LMRCLYCHNR DTWDTHGGKE    50
VTVEDLMKEV VTYRHFMNAS GGGVTASGGE AILQAEFVRD WFRACKKEGI 100
HTCLDTNGFV RRYDPVIDEL LEVTDLVMLD LKQMNDEIHQ NLVGVSNHRT 150
LEFAKYLANK NVKVWIRYVV VPGWSDDDDS AHRLGEFTRD MGNVEKIELL 200
PYHELGKHKW VAMGEEYKLD GVKPPKKETM ERVKGILEQY GHKVMF 246
Length:246
Mass (Da):28,204
Last modified:January 23, 2007 - v2
Checksum:i486E06A9CE97BF37
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG55387.1.
BA000007 Genomic DNA. Translation: BAB34408.1.
PIRiA99752.
G85615.
RefSeqiNP_286777.1. NC_002655.2.
NP_309012.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG55387; AAG55387; Z1246.
BAB34408; BAB34408; BAB34408.
GeneIDi917728.
958863.
KEGGiece:Z1246.
ecs:ECs0985.
PATRICi18351227. VBIEscCol44059_1117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG55387.1 .
BA000007 Genomic DNA. Translation: BAB34408.1 .
PIRi A99752.
G85615.
RefSeqi NP_286777.1. NC_002655.2.
NP_309012.1. NC_002695.1.

3D structure databases

ProteinModelPortali P0A9N6.
SMRi P0A9N6. Positions 2-246.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z1246.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG55387 ; AAG55387 ; Z1246 .
BAB34408 ; BAB34408 ; BAB34408 .
GeneIDi 917728.
958863.
KEGGi ece:Z1246.
ecs:ECs0985.
PATRICi 18351227. VBIEscCol44059_1117.

Phylogenomic databases

eggNOGi COG1180.
HOGENOMi HOG000011458.
KOi K04069.
OMAi QNLIGVP.
OrthoDBi EOG64FKHC.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-983-MONOMER.
ECOO157:PFLA-MONOMER.

Family and domain databases

InterProi IPR006638. Elp3/MiaB/NifB.
IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02493. PFLA. 1 hit.
PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiPFLA_ECO57
AccessioniPrimary (citable) accession number: P0A9N6
Secondary accession number(s): P09374
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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