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P0A9N6

- PFLA_ECO57

UniProt

P0A9N6 - PFLA_ECO57

Protein

Pyruvate formate-lyase 1-activating enzyme

Gene

pflA

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi30 – 301Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi34 – 341Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi37 – 371Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

    GO - Molecular functioni

    1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glucose metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-983-MONOMER.
    ECOO157:PFLA-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
    Alternative name(s):
    Formate-C-acetyltransferase-activating enzyme 1
    PFL-activating enzyme 1
    Gene namesi
    Name:pflA
    Ordered Locus Names:Z1246, ECs0985
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 246245Pyruvate formate-lyase 1-activating enzymePRO_0000200523Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi155864.Z1246.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A9N6.
    SMRiP0A9N6. Positions 2-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1180.
    HOGENOMiHOG000011458.
    KOiK04069.
    OMAiQNLIGVP.
    OrthoDBiEOG64FKHC.

    Family and domain databases

    InterProiIPR006638. Elp3/MiaB/NifB.
    IPR012838. PFL_activating.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02493. PFLA. 1 hit.
    PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A9N6-1 [UniParc]FASTAAdd to Basket

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    MSVIGRIHSF ESCGTVDGPG IRFITFFQGC LMRCLYCHNR DTWDTHGGKE    50
    VTVEDLMKEV VTYRHFMNAS GGGVTASGGE AILQAEFVRD WFRACKKEGI 100
    HTCLDTNGFV RRYDPVIDEL LEVTDLVMLD LKQMNDEIHQ NLVGVSNHRT 150
    LEFAKYLANK NVKVWIRYVV VPGWSDDDDS AHRLGEFTRD MGNVEKIELL 200
    PYHELGKHKW VAMGEEYKLD GVKPPKKETM ERVKGILEQY GHKVMF 246
    Length:246
    Mass (Da):28,204
    Last modified:January 23, 2007 - v2
    Checksum:i486E06A9CE97BF37
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG55387.1.
    BA000007 Genomic DNA. Translation: BAB34408.1.
    PIRiA99752.
    G85615.
    RefSeqiNP_286777.1. NC_002655.2.
    NP_309012.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG55387; AAG55387; Z1246.
    BAB34408; BAB34408; BAB34408.
    GeneIDi917728.
    958863.
    KEGGiece:Z1246.
    ecs:ECs0985.
    PATRICi18351227. VBIEscCol44059_1117.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG55387.1 .
    BA000007 Genomic DNA. Translation: BAB34408.1 .
    PIRi A99752.
    G85615.
    RefSeqi NP_286777.1. NC_002655.2.
    NP_309012.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali P0A9N6.
    SMRi P0A9N6. Positions 2-246.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z1246.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG55387 ; AAG55387 ; Z1246 .
    BAB34408 ; BAB34408 ; BAB34408 .
    GeneIDi 917728.
    958863.
    KEGGi ece:Z1246.
    ecs:ECs0985.
    PATRICi 18351227. VBIEscCol44059_1117.

    Phylogenomic databases

    eggNOGi COG1180.
    HOGENOMi HOG000011458.
    KOi K04069.
    OMAi QNLIGVP.
    OrthoDBi EOG64FKHC.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-983-MONOMER.
    ECOO157:PFLA-MONOMER.

    Family and domain databases

    InterProi IPR006638. Elp3/MiaB/NifB.
    IPR012838. PFL_activating.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02493. PFLA. 1 hit.
    PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiPFLA_ECO57
    AccessioniPrimary (citable) accession number: P0A9N6
    Secondary accession number(s): P09374
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 62 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3