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P0A9N4

- PFLA_ECOLI

UniProt

P0A9N4 - PFLA_ECOLI

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Protein

Pyruvate formate-lyase 1-activating enzyme

Gene

pflA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi34 – 341Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi37 – 371Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

  1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. oxidoreductase activity Source: EcoliWiki

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: EcoliWiki
  2. glucose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:PFLACTENZ-MONOMER.
ECOL316407:JW0885-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 1
PFL-activating enzyme 1
Gene namesi
Name:pflA
Synonyms:act
Ordered Locus Names:b0902, JW0885
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10028. pflA.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 246245Pyruvate formate-lyase 1-activating enzymePRO_0000200522Add
BLAST

Proteomic databases

PaxDbiP0A9N4.
PRIDEiP0A9N4.

Expressioni

Gene expression databases

GenevestigatoriP0A9N4.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ydcYP644554EBI-1114060,EBI-9129853

Protein-protein interaction databases

DIPiDIP-35915N.
IntActiP0A9N4. 19 interactions.
STRINGi511145.b0902.

Structurei

Secondary structure

1
246
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 1410
Beta strandi18 – 2811
Helixi40 – 423
Beta strandi48 – 514
Helixi53 – 608
Helixi61 – 633
Helixi64 – 674
Beta strandi73 – 797
Helixi81 – 844
Helixi85 – 9612
Turni97 – 993
Beta strandi102 – 1065
Helixi115 – 1228
Beta strandi125 – 1306
Helixi136 – 1438
Helixi148 – 16013
Beta strandi164 – 1707
Turni172 – 1743
Helixi178 – 19114
Beta strandi194 – 2018
Helixi207 – 2126
Turni218 – 2214
Helixi227 – 23812
Turni239 – 2413

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C8FX-ray2.25A2-246[»]
3CB8X-ray2.77A2-246[»]
ProteinModelPortaliP0A9N4.
SMRiP0A9N4. Positions 2-246.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9N4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011458.
InParanoidiP0A9N4.
KOiK04069.
OMAiQNLIGVP.
OrthoDBiEOG64FKHC.
PhylomeDBiP0A9N4.

Family and domain databases

InterProiIPR006638. Elp3/MiaB/NifB.
IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9N4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVIGRIHSF ESCGTVDGPG IRFITFFQGC LMRCLYCHNR DTWDTHGGKE
60 70 80 90 100
VTVEDLMKEV VTYRHFMNAS GGGVTASGGE AILQAEFVRD WFRACKKEGI
110 120 130 140 150
HTCLDTNGFV RRYDPVIDEL LEVTDLVMLD LKQMNDEIHQ NLVGVSNHRT
160 170 180 190 200
LEFAKYLANK NVKVWIRYVV VPGWSDDDDS AHRLGEFTRD MGNVEKIELL
210 220 230 240
PYHELGKHKW VAMGEEYKLD GVKPPKKETM ERVKGILEQY GHKVMF
Length:246
Mass (Da):28,204
Last modified:January 23, 2007 - v2
Checksum:i486E06A9CE97BF37
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X08035 Genomic DNA. Translation: CAA30829.1.
U00096 Genomic DNA. Translation: AAC73988.1.
AP009048 Genomic DNA. Translation: BAA35637.1.
PIRiS01789.
RefSeqiNP_415422.1. NC_000913.3.
YP_489174.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73988; AAC73988; b0902.
BAA35637; BAA35637; BAA35637.
GeneIDi12930359.
945517.
KEGGiecj:Y75_p0874.
eco:b0902.
PATRICi32117017. VBIEscCol129921_0933.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X08035 Genomic DNA. Translation: CAA30829.1 .
U00096 Genomic DNA. Translation: AAC73988.1 .
AP009048 Genomic DNA. Translation: BAA35637.1 .
PIRi S01789.
RefSeqi NP_415422.1. NC_000913.3.
YP_489174.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3C8F X-ray 2.25 A 2-246 [» ]
3CB8 X-ray 2.77 A 2-246 [» ]
ProteinModelPortali P0A9N4.
SMRi P0A9N4. Positions 2-246.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-35915N.
IntActi P0A9N4. 19 interactions.
STRINGi 511145.b0902.

Proteomic databases

PaxDbi P0A9N4.
PRIDEi P0A9N4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73988 ; AAC73988 ; b0902 .
BAA35637 ; BAA35637 ; BAA35637 .
GeneIDi 12930359.
945517.
KEGGi ecj:Y75_p0874.
eco:b0902.
PATRICi 32117017. VBIEscCol129921_0933.

Organism-specific databases

EchoBASEi EB0027.
EcoGenei EG10028. pflA.

Phylogenomic databases

eggNOGi COG1180.
HOGENOMi HOG000011458.
InParanoidi P0A9N4.
KOi K04069.
OMAi QNLIGVP.
OrthoDBi EOG64FKHC.
PhylomeDBi P0A9N4.

Enzyme and pathway databases

BioCyci EcoCyc:PFLACTENZ-MONOMER.
ECOL316407:JW0885-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A9N4.
PROi P0A9N4.

Gene expression databases

Genevestigatori P0A9N4.

Family and domain databases

InterProi IPR006638. Elp3/MiaB/NifB.
IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02493. PFLA. 1 hit.
PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structures of Escherichia coli pyruvate formate-lyase and pyruvate-formate-lyase-activating enzyme deduced from the DNA nucleotide sequences."
    Roedel W., Plaga W., Frank R., Knappe J.
    Eur. J. Biochem. 177:153-158(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPFLA_ECOLI
AccessioniPrimary (citable) accession number: P0A9N4
Secondary accession number(s): P09374
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3