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Protein

Phosphocarrier protein NPr

Gene

ptsO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the phosphoenolpyruvate-dependent nitrogen-metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein NPr by enzyme I-Ntr. Phospho-NPr then transfers it to EIIA-Ntr. Could function in the transcriptional regulation of sigma-54 dependent operons in conjunction with the NPr (PtsO) and EIIA-Ntr (PtsN) proteins.

Catalytic activityi

Protein NPr N(pi)-phospho-L-histidine + protein EIIA-Ntr = protein NPr + protein EIIA-Ntr N(tau)-phospho-L-histidine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei16Pros-phosphohistidine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
  • protein phosphorylation Source: EcoCyc
  • response to organonitrogen compound Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Phosphotransferase system

Enzyme and pathway databases

BioCyciEcoCyc:EG12147-MONOMER.
ECOL316407:JW3173-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphocarrier protein NPr (EC:2.7.11.-)
Alternative name(s):
Nitrogen-related HPr
Gene namesi
Name:ptsO
Synonyms:npr, rpoR, yhbK
Ordered Locus Names:b3206, JW3173
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12147. ptsO.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001078901 – 90Phosphocarrier protein NPrAdd BLAST90

Proteomic databases

PaxDbiP0A9N0.
PRIDEiP0A9N0.

Interactioni

Protein-protein interaction databases

BioGridi4261609. 9 interactors.
STRINGi511145.b3206.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5T17NMR-A1-85[»]
ProteinModelPortaliP0A9N0.
SMRiP0A9N0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 90HPrPROSITE-ProRule annotationAdd BLAST89

Sequence similaritiesi

Belongs to the HPr family.Curated
Contains 1 HPr domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41080Z8. Bacteria.
COG1925. LUCA.
HOGENOMiHOG000278398.
InParanoidiP0A9N0.
KOiK08485.
OMAiFIEVETD.
PhylomeDBiP0A9N0.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di3.30.1340.10. 1 hit.
InterProiIPR000032. HPr-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9N0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVKQTVEIT NKLGMHARPA MKLFELMQGF DAEVLLRNDE GTEAEANSVI
60 70 80 90
ALLMLDSAKG RQIEVEATGP QEEEALAAVI ALFNSGFDED
Length:90
Mass (Da):9,810
Last modified:July 19, 2005 - v1
Checksum:i12A8EFEB514F6015
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12684 Genomic DNA. Translation: AAB60167.1.
Z27094 Genomic DNA. Translation: CAA81621.1.
U18997 Genomic DNA. Translation: AAA58008.1.
U00096 Genomic DNA. Translation: AAC76238.1.
AP009048 Genomic DNA. Translation: BAE77250.1.
PIRiI76722.
RefSeqiNP_417673.1. NC_000913.3.
WP_000216791.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76238; AAC76238; b3206.
BAE77250; BAE77250; BAE77250.
GeneIDi947914.
KEGGiecj:JW3173.
eco:b3206.
PATRICi32121832. VBIEscCol129921_3300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12684 Genomic DNA. Translation: AAB60167.1.
Z27094 Genomic DNA. Translation: CAA81621.1.
U18997 Genomic DNA. Translation: AAA58008.1.
U00096 Genomic DNA. Translation: AAC76238.1.
AP009048 Genomic DNA. Translation: BAE77250.1.
PIRiI76722.
RefSeqiNP_417673.1. NC_000913.3.
WP_000216791.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5T17NMR-A1-85[»]
ProteinModelPortaliP0A9N0.
SMRiP0A9N0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261609. 9 interactors.
STRINGi511145.b3206.

Proteomic databases

PaxDbiP0A9N0.
PRIDEiP0A9N0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76238; AAC76238; b3206.
BAE77250; BAE77250; BAE77250.
GeneIDi947914.
KEGGiecj:JW3173.
eco:b3206.
PATRICi32121832. VBIEscCol129921_3300.

Organism-specific databases

EchoBASEiEB2067.
EcoGeneiEG12147. ptsO.

Phylogenomic databases

eggNOGiENOG41080Z8. Bacteria.
COG1925. LUCA.
HOGENOMiHOG000278398.
InParanoidiP0A9N0.
KOiK08485.
OMAiFIEVETD.
PhylomeDBiP0A9N0.

Enzyme and pathway databases

BioCyciEcoCyc:EG12147-MONOMER.
ECOL316407:JW3173-MONOMER.

Miscellaneous databases

PROiP0A9N0.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di3.30.1340.10. 1 hit.
InterProiIPR000032. HPr-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTSO_ECOLI
AccessioniPrimary (citable) accession number: P0A9N0
Secondary accession number(s): P33996, Q2M906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Enzyme I of the sugar PTS has been shown in PubMed:7876255 to phosphorylate NPr of the nitrogen-metabolic PTS, though much less efficiently than it does HPr. This process may link carbon and nitrogen assimilation.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.