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Protein

Phosphocarrier protein NPr

Gene

ptsO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the phosphoenolpyruvate-dependent nitrogen-metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein NPr by enzyme I-Ntr. Phospho-NPr then transfers it to EIIA-Ntr. Could function in the transcriptional regulation of sigma-54 dependent operons in conjunction with the NPr (PtsO) and EIIA-Ntr (PtsN) proteins.

Catalytic activityi

Protein NPr N(pi)-phospho-L-histidine + protein EIIA-Ntr = protein NPr + protein EIIA-Ntr N(tau)-phospho-L-histidine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei16 – 161Pros-phosphohistidine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
  • protein phosphorylation Source: EcoCyc
  • response to organonitrogen compound Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Phosphotransferase system

Enzyme and pathway databases

BioCyciEcoCyc:EG12147-MONOMER.
ECOL316407:JW3173-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphocarrier protein NPr (EC:2.7.11.-)
Alternative name(s):
Nitrogen-related HPr
Gene namesi
Name:ptsO
Synonyms:npr, rpoR, yhbK
Ordered Locus Names:b3206, JW3173
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12147. ptsO.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9090Phosphocarrier protein NPrPRO_0000107890Add
BLAST

Proteomic databases

PaxDbiP0A9N0.

Interactioni

Protein-protein interaction databases

BioGridi4261609. 9 interactions.
STRINGi511145.b3206.

Structurei

3D structure databases

ProteinModelPortaliP0A9N0.
SMRiP0A9N0. Positions 6-89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 9089HPrPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the HPr family.Curated
Contains 1 HPr domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41080Z8. Bacteria.
COG1925. LUCA.
HOGENOMiHOG000278398.
InParanoidiP0A9N0.
KOiK08485.
OMAiMLLESSQ.
OrthoDBiEOG6XDGX2.
PhylomeDBiP0A9N0.

Family and domain databases

Gene3Di3.30.1340.10. 1 hit.
InterProiIPR000032. HPr_prot-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9N0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVKQTVEIT NKLGMHARPA MKLFELMQGF DAEVLLRNDE GTEAEANSVI
60 70 80 90
ALLMLDSAKG RQIEVEATGP QEEEALAAVI ALFNSGFDED
Length:90
Mass (Da):9,810
Last modified:July 19, 2005 - v1
Checksum:i12A8EFEB514F6015
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12684 Genomic DNA. Translation: AAB60167.1.
Z27094 Genomic DNA. Translation: CAA81621.1.
U18997 Genomic DNA. Translation: AAA58008.1.
U00096 Genomic DNA. Translation: AAC76238.1.
AP009048 Genomic DNA. Translation: BAE77250.1.
PIRiI76722.
RefSeqiNP_417673.1. NC_000913.3.
WP_000216791.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76238; AAC76238; b3206.
BAE77250; BAE77250; BAE77250.
GeneIDi947914.
KEGGiecj:JW3173.
eco:b3206.
PATRICi32121832. VBIEscCol129921_3300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12684 Genomic DNA. Translation: AAB60167.1.
Z27094 Genomic DNA. Translation: CAA81621.1.
U18997 Genomic DNA. Translation: AAA58008.1.
U00096 Genomic DNA. Translation: AAC76238.1.
AP009048 Genomic DNA. Translation: BAE77250.1.
PIRiI76722.
RefSeqiNP_417673.1. NC_000913.3.
WP_000216791.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9N0.
SMRiP0A9N0. Positions 6-89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261609. 9 interactions.
STRINGi511145.b3206.

Proteomic databases

PaxDbiP0A9N0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76238; AAC76238; b3206.
BAE77250; BAE77250; BAE77250.
GeneIDi947914.
KEGGiecj:JW3173.
eco:b3206.
PATRICi32121832. VBIEscCol129921_3300.

Organism-specific databases

EchoBASEiEB2067.
EcoGeneiEG12147. ptsO.

Phylogenomic databases

eggNOGiENOG41080Z8. Bacteria.
COG1925. LUCA.
HOGENOMiHOG000278398.
InParanoidiP0A9N0.
KOiK08485.
OMAiMLLESSQ.
OrthoDBiEOG6XDGX2.
PhylomeDBiP0A9N0.

Enzyme and pathway databases

BioCyciEcoCyc:EG12147-MONOMER.
ECOL316407:JW3173-MONOMER.

Miscellaneous databases

PROiP0A9N0.

Family and domain databases

Gene3Di3.30.1340.10. 1 hit.
InterProiIPR000032. HPr_prot-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant."
    Powell B.S., Court D.L., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier M.H. Jr., Reizer J.
    J. Biol. Chem. 270:4822-4839(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Molecular analysis of the operon which encodes the RNA polymerase sigma factor sigma 54 of Escherichia coli."
    Jones D.H.A., Franklin C.F.H., Thomas C.M.
    Microbiology 140:1035-1043(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Plunkett G. III
    Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPTSO_ECOLI
AccessioniPrimary (citable) accession number: P0A9N0
Secondary accession number(s): P33996, Q2M906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: January 20, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Enzyme I of the sugar PTS has been shown in PubMed:7876255 to phosphorylate NPr of the nitrogen-metabolic PTS, though much less efficiently than it does HPr. This process may link carbon and nitrogen assimilation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.