P0A9N0 (PTSO_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphocarrier protein NPr
EC=2.7.11.-

Alternative name(s):
Nitrogen-related HPr

Gene names

Name:	ptsO
Synonyms:	npr, rpoR, yhbK
Ordered Locus Names:	b3206, JW3173

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	90 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the phosphoenolpyruvate-dependent nitrogen-metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein NPr by enzyme I-Ntr. Phospho-NPr then transfers it to EIIA-Ntr. Could function in the transcriptional regulation of sigma-54 dependent operons in conjunction with the NPr (PtsO) and EIIA-Ntr (PtsN) proteins.
Catalytic activity	Protein NPr N(pi)-phospho-L-histidine + protein EIIA-Ntr = protein NPr + protein EIIA-Ntr N(tau)-phospho-L-histidine.
Subcellular location	Cytoplasm Probable.
Miscellaneous	Enzyme I of the sugar PTS has been shown in Ref.1 to phosphorylate NPr of the nitrogen-metabolic PTS, though much less efficiently than it does HPr. This process may link carbon and nitrogen assimilation.
Sequence similarities	Belongs to the HPr family. Contains 1 HPr domain.

Ontologies

Keywords
Biological process	Phosphotransferase system
Cellular component	Cytoplasm
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 90	90	Phosphocarrier protein NPr		PRO_0000107890
Regions
	Domain	2 – 90	89	HPr
Sites
	Active site	16	1	Pros-phosphohistidine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A9N0 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 12A8EFEB514F6015	FASTA	90	9,810
	10 20 30 40 50 60 MTVKQTVEIT NKLGMHARPA MKLFELMQGF DAEVLLRNDE GTEAEANSVI ALLMLDSAKG 70 80 90 RQIEVEATGP QEEEALAAVI ALFNSGFDED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant." Powell B.S., Court D.L., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier M.H. Jr., Reizer J. J. Biol. Chem. 270:4822-4839(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Molecular analysis of the operon which encodes the RNA polymerase sigma factor sigma 54 of Escherichia coli." Jones D.H.A., Franklin C.F.H., Thomas C.M. Microbiology 140:1035-1043(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	Plunkett G. III Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12684 Genomic DNA. Translation: AAB60167.1. Z27094 Genomic DNA. Translation: CAA81621.1. U18997 Genomic DNA. Translation: AAA58008.1. U00096 Genomic DNA. Translation: AAC76238.1. AP009048 Genomic DNA. Translation: BAE77250.1.
PIR	I76722.
RefSeq	NP_417673.1. NC_000913.2. YP_491391.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A9N0.
SMR	P0A9N0. Positions 6-89.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b3206.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76238; AAC76238; b3206. BAE77250; BAE77250; BAE77250.
GeneID	12932615. 947914.
KEGG	ecj:Y75_p3126. eco:b3206.
PATRIC	32121832. VBIEscCol129921_3300.
Organism-specific databases
EchoBASE	EB2067.
EcoGene	EG12147. ptsO.
Phylogenomic databases
eggNOG	COG1925.
HOGENOM	HOG000278398.
KO	K08485.
OMA	DRRINAK.
ProtClustDB	PRK10897.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12147-MONOMER. ECOL316407:JW3173-MONOMER.
Gene expression databases
Genevestigator	P0A9N0.
Family and domain databases
Gene3D	3.30.1340.10. 1 hit.
InterPro	IPR001020. PTS_HPr_His_P_site. IPR005698. PTS_HPr_prot. IPR000032. PTS_HPr_prot-like. IPR002114. PTS_HPr_Ser_P_site. [Graphical view]
Pfam	PF00381. PTS-HPr. 1 hit. [Graphical view]
PRINTS	PR00107. PHOSPHOCPHPR.
SUPFAM	SSF55594. HPr_protein. 1 hit.
TIGRFAMs	TIGR01003. PTS_HPr_family. 1 hit.
PROSITE	PS51350. PTS_HPR_DOM. 1 hit. PS00369. PTS_HPR_HIS. 1 hit. PS00589. PTS_HPR_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTSO_ECOLI

Accession

Primary (citable) accession number: P0A9N0
Secondary accession number(s): P33996, Q2M906

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program