Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphocarrier protein NPr

Gene

ptsO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the phosphoenolpyruvate-dependent nitrogen-metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein NPr by enzyme I-Ntr. Phospho-NPr then transfers it to EIIA-Ntr. Could function in the transcriptional regulation of sigma-54 dependent operons in conjunction with the NPr (PtsO) and EIIA-Ntr (PtsN) proteins.

Miscellaneous

Enzyme I of the sugar PTS has been shown in PubMed:7876255 to phosphorylate NPr of the nitrogen-metabolic PTS, though much less efficiently than it does HPr. This process may link carbon and nitrogen assimilation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei16Pros-phosphohistidine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
  • protein phosphorylation Source: EcoCyc
  • response to organonitrogen compound Source: EcoCyc

Keywordsi

Biological processPhosphotransferase system

Enzyme and pathway databases

BioCyciEcoCyc:EG12147-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphocarrier protein NPr
Alternative name(s):
Nitrogen-related HPr
Gene namesi
Name:ptsO
Synonyms:npr, rpoR, yhbK
Ordered Locus Names:b3206, JW3173
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12147 ptsO

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001078901 – 90Phosphocarrier protein NPrAdd BLAST90

Proteomic databases

PaxDbiP0A9N0
PRIDEiP0A9N0

Interactioni

Protein-protein interaction databases

BioGridi4261609, 13 interactors
STRINGi316385.ECDH10B_3380

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5T17NMR-A1-85[»]
5T1NNMR-A1-85[»]
5T1ONMR-A1-85[»]
ProteinModelPortaliP0A9N0
SMRiP0A9N0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 90HPrPROSITE-ProRule annotationAdd BLAST89

Sequence similaritiesi

Belongs to the HPr family.Curated

Phylogenomic databases

eggNOGiENOG41080Z8 Bacteria
COG1925 LUCA
HOGENOMiHOG000278398
InParanoidiP0A9N0
KOiK08485
OMAiYIEVEAT
PhylomeDBiP0A9N0

Family and domain databases

CDDicd00367 PTS-HPr_like, 1 hit
Gene3Di3.30.1340.10, 1 hit
InterProiView protein in InterPro
IPR000032 HPr-like
IPR035895 HPr-like_sf
IPR001020 PTS_HPr_His_P_site
IPR002114 PTS_HPr_Ser_P_site
PfamiView protein in Pfam
PF00381 PTS-HPr, 1 hit
PRINTSiPR00107 PHOSPHOCPHPR
SUPFAMiSSF55594 SSF55594, 1 hit
TIGRFAMsiTIGR01003 PTS_HPr_family, 1 hit
PROSITEiView protein in PROSITE
PS51350 PTS_HPR_DOM, 1 hit
PS00369 PTS_HPR_HIS, 1 hit
PS00589 PTS_HPR_SER, 1 hit

Sequencei

Sequence statusi: Complete.

P0A9N0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVKQTVEIT NKLGMHARPA MKLFELMQGF DAEVLLRNDE GTEAEANSVI
60 70 80 90
ALLMLDSAKG RQIEVEATGP QEEEALAAVI ALFNSGFDED
Length:90
Mass (Da):9,810
Last modified:July 19, 2005 - v1
Checksum:i12A8EFEB514F6015
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12684 Genomic DNA Translation: AAB60167.1
Z27094 Genomic DNA Translation: CAA81621.1
U18997 Genomic DNA Translation: AAA58008.1
U00096 Genomic DNA Translation: AAC76238.1
AP009048 Genomic DNA Translation: BAE77250.1
PIRiI76722
RefSeqiNP_417673.1, NC_000913.3
WP_000216791.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76238; AAC76238; b3206
BAE77250; BAE77250; BAE77250
GeneIDi947914
KEGGiecj:JW3173
eco:b3206
PATRICifig|1411691.4.peg.3525

Entry informationi

Entry nameiPTSO_ECOLI
AccessioniPrimary (citable) accession number: P0A9N0
Secondary accession number(s): P33996, Q2M906
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health