P0A9M9 (PTA_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphate acetyltransferase
EC=2.3.1.8

Alternative name(s):
Phosphotransacetylase

Gene names

Name:	pta
Ordered Locus Names:	SF2373, S2508

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in acetate metabolism By similarity.
Catalytic activity	Acetyl-CoA + phosphate = CoA + acetyl phosphate.
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 2/2.
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm Potential.
Domain	The N-terminal region seems to be important for proper quaternary structure. The C-terminal region contains the substrate-binding site By similarity.
Sequence similarities	In the N-terminal section; belongs to the CobB/CobQ family. In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphate acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 714	713	Phosphate acetyltransferase		PRO_0000179139
Regions
	Region	391 – 714	324	Phosphate acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P0A9M9 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: B45AF0C93494A04C	FASTA	714	77,172
	10 20 30 40 50 60 MSRIIMLIPT GTSVGLTSVS LGVIRAMERK GVRLSVFKPI AQPRTGGDAP DQTTTIVRAN 70 80 90 100 110 120 SSTTTAAEPL KMSYVEGLLS SNQKDVLMEE IVANYHANTK DAEVVLVEGL VPTRKHQFAQ 130 140 150 160 170 180 SLNYEIAKTL NAEIVFVMSQ GTDTPEQLKE RIELTRNSFG GAKNTNITGV IVNKLNAPVD 190 200 210 220 230 240 EQGRTRPDLS EIFDDSSKAK VNNVDPAKLQ ESSPLPVLGA VPWSFDLIAT RAIDMARHLN 250 260 270 280 290 300 ATIINEGDIN TRRVKSVTFC ARSIPHMLEH FRAGSLLVTS ADRPDVLVAA CLAAMNGVEI 310 320 330 340 350 360 GALLLTGGYE MDARISKLCE RAFATGLPVF MVNTNTWQTS LSLQSFNLEV PVDDHERIEK 370 380 390 400 410 420 VQEYVANYIN ADWIESLTAT SERSRRLSPP AFRYQLTELA RKAGKRIVLP EGDEPRTVKA 430 440 450 460 470 480 AAICAERGIA TCVLLGNPAE INRVAASQGV ELGAGIEIVD PEVVRESYVG RLVELRKNKG 490 500 510 520 530 540 MTETVAREQL EDNVVLGTLM LEQDEVDGLV SGAVHTTANT IRPPLQLIKT APGSSLVSSV 550 560 570 580 590 600 FFMLLPEQVY VYGDCAINPD PTAEQLAEIA IQSADSAAAF GIEPRVAMLS YSTGTSGAGS 610 620 630 640 650 660 DVEKVREATR LAQEKRPDLM IDGPLQYDAA VMADVAKSKA PNSPVAGRAT VFIFPDLNTG 670 680 690 700 710 NTTYKAVQRS ADLISIGPML QGMRKPVNDL SRGALVDDIV YTIALTAIQS AQQQ

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN43886.1. AE014073 Genomic DNA. Translation: AAP17704.1.
RefSeq	NP_708179.1. NC_004337.2. NP_837894.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0A9M9.
SMR	P0A9M9. Positions 3-37, 230-353, 390-714.
ModBase	Search...
Proteomic databases
PRIDE	P0A9M9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000088644; EBESCP00000085466; EBESCG00000087688. EBESCT00000091374; EBESCP00000088010; EBESCG00000090418.
GeneID	1027244. 1078796.
GenomeReviews	Gene locus SF2373 in contig AE005674_GR. Gene locus S2508 in contig AE014073_GR.
KEGG	sfl:SF2373. sfx:S2508.
PATRIC	18706611. VBIShiFle31049_2781.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008963.
HOGENOM	HBG576804.
OMA	KPIAQPH.
ProtClustDB	PRK05632.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN43886.1-MONOMER.
Family and domain databases
InterPro	IPR010766. DRTGG. IPR016475. P-Actrans_bac. IPR004614. P_AcTrfase. IPR002505. PTA_PTB. [Graphical view]
KO	K13788.
Pfam	PF07085. DRTGG. 1 hit. PF01515. PTA_PTB. 1 hit. [Graphical view]
PIRSF	PIRSF006107. PhpActrans_proteobac. 1 hit.
TIGRFAMs	TIGR00651. Pta. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PTA_SHIFL

Accession

Primary (citable) accession number: P0A9M9
Secondary accession number(s): P39184 , P78091, P78189, P78190, Q9EUP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program