Reviewed, UniProtKB/Swiss-Prot P0A9M8 (PTA_ECOLI)

Names and origin

Protein names

Recommended name:
    Phosphate acetyltransferase
    EC=2.3.1.8
Alternative name(s):
    Phosphotransacetylase

Gene names

Name:	pta
Ordered Locus Names:	b2297, JW2294

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + phosphate = CoA + acetyl phosphate.
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 2/2.
Subcellular location	Cytoplasm Potential.
Sequence similarities	In the N-terminal section; belongs to the cobB/cobQ family. In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphate acetyltransferase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
chpS	P08365	1	EBI-555015,EBI-556499
napD	P0A9I5	1	EBI-555015,EBI-554985
yjgH	P39332	1	EBI-555015,EBI-561380

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.2
	Chain	2 – 714	713	Phosphate acetyltransferase		PRO_0000179129
Regions
	Region	391 – 714	324	Phosphate acetyltransferase
Experimental info
	Sequence conflict	20 – 50	31	SLGVI…GGDAP → AWRDPCNGTQRRSSERFQTY RSAAYRWRCA in BAA04663. Ref.2
	Sequence conflict	208 – 209	2	KL → NV Ref.1
	Sequence conflict	208 – 209	2	KL → NV Ref.2
	Sequence conflict	263 – 271	9	SIPHMLEHF → QHSAHAGAL in BAA04663. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A9M8-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: B45AF0C93494A04C	FASTA	714	77,172
	10 20 30 40 50 60 MSRIIMLIPT GTSVGLTSVS LGVIRAMERK GVRLSVFKPI AQPRTGGDAP DQTTTIVRAN 70 80 90 100 110 120 SSTTTAAEPL KMSYVEGLLS SNQKDVLMEE IVANYHANTK DAEVVLVEGL VPTRKHQFAQ 130 140 150 160 170 180 SLNYEIAKTL NAEIVFVMSQ GTDTPEQLKE RIELTRNSFG GAKNTNITGV IVNKLNAPVD 190 200 210 220 230 240 EQGRTRPDLS EIFDDSSKAK VNNVDPAKLQ ESSPLPVLGA VPWSFDLIAT RAIDMARHLN 250 260 270 280 290 300 ATIINEGDIN TRRVKSVTFC ARSIPHMLEH FRAGSLLVTS ADRPDVLVAA CLAAMNGVEI 310 320 330 340 350 360 GALLLTGGYE MDARISKLCE RAFATGLPVF MVNTNTWQTS LSLQSFNLEV PVDDHERIEK 370 380 390 400 410 420 VQEYVANYIN ADWIESLTAT SERSRRLSPP AFRYQLTELA RKAGKRIVLP EGDEPRTVKA 430 440 450 460 470 480 AAICAERGIA TCVLLGNPAE INRVAASQGV ELGAGIEIVD PEVVRESYVG RLVELRKNKG 490 500 510 520 530 540 MTETVAREQL EDNVVLGTLM LEQDEVDGLV SGAVHTTANT IRPPLQLIKT APGSSLVSSV 550 560 570 580 590 600 FFMLLPEQVY VYGDCAINPD PTAEQLAEIA IQSADSAAAF GIEPRVAMLS YSTGTSGAGS 610 620 630 640 650 660 DVEKVREATR LAQEKRPDLM IDGPLQYDAA VMADVAKSKA PNSPVAGRAT VFIFPDLNTG 670 680 690 700 710 NTTYKAVQRS ADLISIGPML QGMRKPVNDL SRGALVDDIV YTIALTAIQS AQQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli." Kakuda H., Hosono K., Shiroishi K., Ichihara S. J. Biochem. 116:916-922(1994) [PubMed: 7883769] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-12. Strain: K12 / KH131.
[2]	"Nucleotide sequence of the phosphotransacetylase gene of Escherichia coli strain K12." Matsuyama A., Yamamoto-Otake H., Hewitt J., McGillivray R.T.A., Nakano E. Biochim. Biophys. Acta 1219:559-562(1994) [PubMed: 7918659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9. Strain: K12 / 1100.
[3]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene." Matsuyama A., Yamamoto H., Nakano E. J. Bacteriol. 171:577-580(1989) [PubMed: 2536666] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5. Strain: K12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17576 Genomic DNA. Translation: BAA04502.1. D21123 Genomic DNA. Translation: BAA04663.1. U00096 Genomic DNA. Translation: AAC75357.1. AP009048 Genomic DNA. Translation: BAA16136.1.
PIR	G65001. JX0357. S50130.
RefSeq	AP_002897.1. NP_416800.1.
3D structure databases
SMR	P0A9M8. Positions 2-243, 233-345, 392-713.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A9M8. 26 interactions.
STRING	P0A9M8.
PTM databases
PhosSite	P0A9M8.
2-D gel databases
SWISS-2DPAGE	P0A9M8.
Proteomic databases
PRIDE	P0A9M8.
Genome annotation databases
GeneID	946778.
GenomeReviews	Gene locus JW2294 in contig AP009048_GR. Gene locus b2297 in contig U00096_GR.
KEGG	ecj:JW2294. eco:b2297.
Organism-specific databases
EchoBASE	EB4147.
EcoGene	EG20173. pta.
CMR	Search...
Phylogenomic databases
eggNOG	COG0857.
HOGENOM	HBG576804.
OMA	KPIAQPH.
Enzyme and pathway databases
BioCyc	EcoCyc:PHOSACETYLTRANS-MONOMER. ECOL168927:B2297-MONOMER. MetaCyc:PHOSACETYLTRANS-MONOMER.
Gene expression databases
Genevestigator	P0A9M8.
Family and domain databases
InterPro	IPR010766. DRTGG. IPR016475. P-Actrans_bac. IPR004614. P_AcTrfase. IPR002505. PTA_PTB. [Graphical view]
Pfam	PF07085. DRTGG. 1 hit. PF01515. PTA_PTB. 1 hit. [Graphical view]
PIRSF	PIRSF006107. PhpActrans_proteobac. 1 hit.
TIGRFAMs	TIGR00651. pta. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PTA_ECOLI

Accession

Primary (citable) accession number: P0A9M8
Secondary accession number(s): P39184 , P78091, P78189, P78190, Q9EUP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)