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Protein

Xanthine phosphoribosyltransferase

Gene

gpt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts on guanine, xanthine and to a lesser extent hypoxanthine.1 Publication

Catalytic activityi

XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine.

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Enzyme regulationi

Inhibited by thioguanine, GMP and, to a lesser extent, by thioxanthine, azaxanthine and azaguanine.1 Publication

Kineticsi

  1. KM=4.3 µM for guanine2 Publications
  2. KM=30.5 µM for xanthine2 Publications
  3. KM=90.8 µM for hypoxanthine2 Publications

    Pathwayi: XMP biosynthesis via salvage pathway

    This protein is involved in step 1 of the subpathway that synthesizes XMP from xanthine.
    Proteins known to be involved in this subpathway in this organism are:
    1. Xanthine phosphoribosyltransferase (gpt)
    This subpathway is part of the pathway XMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from xanthine, the pathway XMP biosynthesis via salvage pathway and in Purine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei695-phosphoribose 1-diphosphate1
    Metal bindingi89Magnesium1 Publication1
    Binding sitei92Xanthine1
    Binding sitei135Xanthine; via amide nitrogen and carbonyl oxygen1

    GO - Molecular functioni

    • hypoxanthine phosphoribosyltransferase activity Source: EcoCyc
    • magnesium ion binding Source: UniProtKB-HAMAP
    • xanthine phosphoribosyltransferase activity Source: EcoCyc

    GO - Biological processi

    • GMP salvage Source: EcoCyc
    • IMP salvage Source: EcoCyc
    • XMP salvage Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:GPT-MONOMER.
    ECOL316407:JW0228-MONOMER.
    MetaCyc:GPT-MONOMER.
    BRENDAi2.4.2.22. 2026.
    UniPathwayiUPA00602; UER00658.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xanthine phosphoribosyltransferase (EC:2.4.2.22)
    Alternative name(s):
    Xanthine-guanine phosphoribosyltransferase
    Short name:
    XGPRT
    Gene namesi
    Name:gpt
    Synonyms:gpp, gxu
    Ordered Locus Names:b0238, JW0228
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10414. gpt.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: UniProtKB
    • plasma membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi59C → A: No effect on catalytic activity; increased stability. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001396661 – 152Xanthine phosphoribosyltransferaseAdd BLAST152

    Proteomic databases

    EPDiP0A9M5.
    PaxDbiP0A9M5.
    PRIDEiP0A9M5.

    2D gel databases

    SWISS-2DPAGEP0A9M5.

    Interactioni

    Subunit structurei

    Homotetramer.2 Publications

    Protein-protein interaction databases

    BioGridi4260989. 8 interactors.
    IntActiP0A9M5. 1 interactor.
    STRINGi511145.b0238.

    Structurei

    Secondary structure

    1152
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 6Combined sources3
    Helixi9 – 23Combined sources15
    Helixi26 – 28Combined sources3
    Beta strandi30 – 36Combined sources7
    Turni37 – 39Combined sources3
    Helixi40 – 50Combined sources11
    Beta strandi55 – 61Combined sources7
    Beta strandi72 – 75Combined sources4
    Beta strandi78 – 80Combined sources3
    Beta strandi84 – 91Combined sources8
    Helixi97 – 103Combined sources7
    Beta strandi107 – 114Combined sources8
    Helixi116 – 121Combined sources6
    Beta strandi123 – 128Combined sources6
    Beta strandi134 – 136Combined sources3
    Helixi138 – 140Combined sources3
    Beta strandi141 – 145Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1A95X-ray2.00A/B/C/D1-152[»]
    1A96X-ray2.00A/B/C/D1-152[»]
    1A97X-ray2.60A/B/C/D3-150[»]
    1A98X-ray2.25A/B1-152[»]
    1NULX-ray1.80A/B1-152[»]
    ProteinModelPortaliP0A9M5.
    SMRiP0A9M5.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A9M5.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni37 – 385-phosphoribose 1-diphosphate binding2
    Regioni92 – 965-phosphoribose 1-diphosphate binding5

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0503. LUCA.
    HOGENOMiHOG000226805.
    InParanoidiP0A9M5.
    KOiK00769.
    OMAiFHRDCRA.
    PhylomeDBiP0A9M5.

    Family and domain databases

    CDDicd06223. PRTases_typeI. 1 hit.
    Gene3Di3.40.50.2020. 1 hit.
    HAMAPiMF_01903. XGPRT. 1 hit.
    InterProiIPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR023747. Xanthine_Guanine_PRibTrfase.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A9M5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSEKYIVTWD MLQIHARKLA SRLMPSEQWK GIIAVSRGGL VPGALLAREL
    60 70 80 90 100
    GIRHVDTVCI SSYDHDNQRE LKVLKRAEGD GEGFIVIDDL VDTGGTAVAI
    110 120 130 140 150
    REMYPKAHFV TIFAKPAGRP LVDDYVVDIP QDTWIEQPWD MGVVFVPPIS

    GR
    Length:152
    Mass (Da):16,971
    Last modified:July 21, 1986 - v1
    Checksum:iF0AD813127E7200D
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti122V → G in AAA23933 (PubMed:3540961).Curated1

    Mass spectrometryi

    Molecular mass is 16805 Da from positions 1 - 152. Determined by API. The measured mass is that of mutant Ala-59.1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X00221 Genomic DNA. Translation: CAA25040.1.
    X00222 Genomic DNA. Translation: CAA25041.1.
    M13422 Genomic DNA. Translation: AAA23928.1.
    M12907 Genomic DNA. Translation: AAA23932.1.
    M15035 Genomic DNA. Translation: AAA23933.1.
    U70214 Genomic DNA. Translation: AAB08658.1.
    U00096 Genomic DNA. Translation: AAC73342.1.
    AP009048 Genomic DNA. Translation: BAA77907.1.
    M10382 Genomic DNA. Translation: AAA23931.1.
    PIRiA00587. RTECGX.
    RefSeqiNP_414773.1. NC_000913.3.
    WP_001291990.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73342; AAC73342; b0238.
    BAA77907; BAA77907; BAA77907.
    GeneIDi944817.
    KEGGiecj:JW0228.
    eco:b0238.
    PATRICi32115591. VBIEscCol129921_0240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X00221 Genomic DNA. Translation: CAA25040.1.
    X00222 Genomic DNA. Translation: CAA25041.1.
    M13422 Genomic DNA. Translation: AAA23928.1.
    M12907 Genomic DNA. Translation: AAA23932.1.
    M15035 Genomic DNA. Translation: AAA23933.1.
    U70214 Genomic DNA. Translation: AAB08658.1.
    U00096 Genomic DNA. Translation: AAC73342.1.
    AP009048 Genomic DNA. Translation: BAA77907.1.
    M10382 Genomic DNA. Translation: AAA23931.1.
    PIRiA00587. RTECGX.
    RefSeqiNP_414773.1. NC_000913.3.
    WP_001291990.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1A95X-ray2.00A/B/C/D1-152[»]
    1A96X-ray2.00A/B/C/D1-152[»]
    1A97X-ray2.60A/B/C/D3-150[»]
    1A98X-ray2.25A/B1-152[»]
    1NULX-ray1.80A/B1-152[»]
    ProteinModelPortaliP0A9M5.
    SMRiP0A9M5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260989. 8 interactors.
    IntActiP0A9M5. 1 interactor.
    STRINGi511145.b0238.

    2D gel databases

    SWISS-2DPAGEP0A9M5.

    Proteomic databases

    EPDiP0A9M5.
    PaxDbiP0A9M5.
    PRIDEiP0A9M5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73342; AAC73342; b0238.
    BAA77907; BAA77907; BAA77907.
    GeneIDi944817.
    KEGGiecj:JW0228.
    eco:b0238.
    PATRICi32115591. VBIEscCol129921_0240.

    Organism-specific databases

    EchoBASEiEB0409.
    EcoGeneiEG10414. gpt.

    Phylogenomic databases

    eggNOGiCOG0503. LUCA.
    HOGENOMiHOG000226805.
    InParanoidiP0A9M5.
    KOiK00769.
    OMAiFHRDCRA.
    PhylomeDBiP0A9M5.

    Enzyme and pathway databases

    UniPathwayiUPA00602; UER00658.
    BioCyciEcoCyc:GPT-MONOMER.
    ECOL316407:JW0228-MONOMER.
    MetaCyc:GPT-MONOMER.
    BRENDAi2.4.2.22. 2026.

    Miscellaneous databases

    EvolutionaryTraceiP0A9M5.
    PROiP0A9M5.

    Family and domain databases

    CDDicd06223. PRTases_typeI. 1 hit.
    Gene3Di3.40.50.2020. 1 hit.
    HAMAPiMF_01903. XGPRT. 1 hit.
    InterProiIPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR023747. Xanthine_Guanine_PRibTrfase.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiXGPT_ECOLI
    AccessioniPrimary (citable) accession number: P0A9M5
    Secondary accession number(s): P00501
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: November 2, 2016
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.